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رویانزایی بدنی گیاه دارویی سرخارگل (Echinacea purpurea (L.) Moench) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 8، دوره 28، شماره 1 - شماره پیاپی 55، شهریور 1399، صفحه 91-101 اصل مقاله (847.56 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2020.342034.1360 | ||
| نویسندگان | ||
| محدثه حجازی1؛ مریم دهستانی اردکانی* 2؛ کاظم کمالی3؛ محسن رشیدی4 | ||
| 1دانش آموخته کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان | ||
| 2استادیار گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه اردکان، یزد، ایران | ||
| 3دانشیار، گروه علوم خاک‌شناسی، دانشکده کشاورزی و منابع طبیعی، دانشگاه یزد | ||
| 4دانش آموخته کارشناسی ارشد، گروه علوم جانوری، دانشکده پیرادامپزشکی، دانشگاه اردکان | ||
| چکیده | ||
| سرخارگل (Echinacea purpurea (L.) Moench) یک گونه گیاهی با ارزش دارویی بالا میباشد. بهمنظور بهینهسازی پروتکل رویانزایی بدنی ریزنمونههای برگی گیاه سرخارگل از 18 تیمار ترکیبی شامل تنظیم کنندههای رشد شامل بنزیل آدنین (BA) در سه سطح (3، 4، 5 میلیگرم در لیتر)، نفتالین استیک اسید (NAA) در سه سطح (1/0، 2/0 و 5/0 میلیگرم در لیتر)، همراه و یا بدون یک گرم در لیتر زغال فعال، 50 میلیلیتر در لیتر شیره نارگیل و 50 میلیگرم در لیتر کازئینهیدرولیزات در محیط کشت MS استفاده شد. داده‌های بدست‌آمده در قالب طرح کاملا تصادفی در سه تکرار مورد تجزیه واریانس قرار گرفتند و مقایسه میانگین تیمارها به روش توکی انجام شد. نتایج نشان داد که رویانزایی با تولید کالوس بهصورت غیرمستقیم اتفاق افتاد. کالوسهای رویانزا به رنگ سبز تیره، منسجم و با رشد سریعتر بودند. بیشترین رویانزایی کالوسها (100 درصد) و نیز باززایی رویان در تیمارهای ترکیب BA و NAA همراه با زغال فعال، شیره نارگیل و کازئین هیدرولیزات حاصل شد. در حالیکه تیمارهای تنظیم‌کننده رشد فاقد شیره نارگیل و کازئین هیدرولیزات موفق به تولید رویان بدنی نشدند. بیشترین میزان تولید شاخساره (3/43 عدد) در تیمار ترکیبی 3 میلیگرم در لیتر BA+1/0 میلیگرم در لیتر NAA+1 میلیگرم در لیتر زغال فعال+50 میلیلیتر در لیتر شیره نارگیل+50 میلیگرم در لیتر کازئینهیدرولیزات مشاهده شد. بهطورکلی برای رویانزایی بدنی سرخارگل، استفاده از سطوح بالای BA و مقادیر اندک NAA به همراه شیرهنارگیل و کازئینهیدرولیزات توصیه میگردد. | ||
| کلیدواژه‌ها | ||
| بنزیل آدنین؛ شیره نارگیل؛ کازئین هیدرولیزات؛ کالوس؛ نفتالین استیک اسید | ||
| عنوان مقاله [English] | ||
| Somatic embryogenesis of medicinal plant of purple cone flower (Echinacea purpurea (L.) Moench) | ||
| نویسندگان [English] | ||
| Mohadeseh Hejazi1؛ Maryam Dehestani-Ardakani2؛ Kazem Kamali aliabad3؛ Mohsen Rashidi4 | ||
| 1M.Sc. Graduated, Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, I.R. Iran | ||
| 2Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran | ||
| 3Assoc. Prof., Department of Soil Science, Faculty of Agriculture and Natural Resources, Yazd University, Yazd, I.R. Iran. | ||
| 4M.Sc. Graduated, Department of Animal Sciences, Faculty of Pediatrics, Ardakan University, Ardakan, I.R. Iran. | ||
| چکیده [English] | ||
| Purple coneflower (Echinacea purpurea (L.) Moench) is a plant species of high medical value. Somatic embryogenesis is considered to be the most efficient micropropagation technique. Considering the importance of this plant, the current study has been performed with the aim of optimizing somatic embryogenic of that. Embryogenic callus induction and embryogenesis were investigated using different concentrations of plant growth regulations including Benzyl adenine (BA) in three levels (3, 4 and 5 mg/l), Naphtalene Acetic Acid (NAA) at three levels (0.1, 0.2 and 0.5 mg/l) with or without 1 g/l active charchoal, 50 ml/l coconut milkand 50 mg/l casein hydrolysate in leaf explants in MS culture medium in a completely randomized design with three replications. Induction of embryogenesis occurred indirectly with callus formation. Embryogenic calli were dark green, coherent with faster growth. The highest percentage of embryogenic callus (100 %) and also regeneration of callus (plantlents) were obtained in combination of BA and NAA with active charcoal, coconut milk and casein hydrolysate treatmens. Whereas the combination treatments of plant growth regulators without coconut milk and casein hydrolysate failed to produce somatic embryos. The maximum number of shoots (43.3 shoots) was observed in 3 mg/l BA + 0.1 mg/l NAA + 1 mg/l activate charcoal + 50 ml/l coconut milk + 50 mg/l casein hydrolysate treatment. | ||
| کلیدواژه‌ها [English] | ||
| Benzyl adenine, Coconut milk, Casein hydrolysate, Naphtalene Acetic Acid | ||
| مراجع | ||
|
- Abbasi, B.H., Saxena, P.K., Murch, S.J. and Liu, C.-Z. 2007. Echinacea biotechnology: challenges and opportunities. In Vitro Cell Development Biology Plant, 43: 481–492. - Ahmad, K.M., Zafar, Z. and Mirza, J.I. 2017. Effect of plant growth regulators on shoot organogenesis and somatic embryogenesis of Echinacea purpurea L. Pakistan journal of life and social Sciences, 15(2): 107-113. - Annadana, S., Radamaker, W., Rammana, M., Udayakumar, M. and de Jong, J. 2000. Response of stem expiants to screening and expiant source as a basis for methodical advancing regener? ation protocols for for chrysanthemum. Plant Cell Tissue and Organ Culture, 62: 47-55. - Arvin, M.J. 2002. Tissue culture of wood trees. Shahid Bahonar univ. of Kerman. univ. Press. 279 pp. (In Persian). - Bagheri, A., Ziaratnia, M. and Hossaini, M. 2004. Tissue culture of trees. univ. of Ferdowsii, univ. Press. Mashhad, Iran. 205 pp. (In Persian). - Chae, W.B., Choi, G.W. and Chung, I.S. 2004. Plant regeneration depending on expiant type in Chrysanthmum coronarium L. Journal of Plant Biotechnology, 6: 253-258. - Choffe, K.L., Victor, J.M., Murch, S.J. and Saxena, P.K. 2000. In vitro regeneration of Echinacea purpurea L.: direct somatic embryogenesis and indirect shoot organogenesis in petiole culture. In Vitro Cellular & Developmental Biology-Plant, 36(1): 30-36. - Ghasemian, Kh, Nazeri, S., Chehregani Rad, A., Mirzaie Asl, A. 2012. The Stages of Somatic Embryogenesis Derived from Zygotic Embryo of Dorema ammoniacum D.. Journal of Cell & Tissue, 3(1): 21-27. (In Persian) - Gruenwald, J., Brendler, T. and Jaenicke, C. 1999. DDR for herbal medicines. Medical Economics Co., New Jersy, USA.Li, Th.S.C. 1998. Echinacea: Cultivation and medicinal value. Hort. Technology, 8: 122-129. - Hunault, G., Desmarest, P., Manoir, J.D. 1989. Foeniculum vulgare Miller: cell culture, regeneration and the production of anethole In: Bajaj YPS Ed. Biotechnology in Agriculture and Forestry, Medicinal and Aromatic Plants. Berlin, Germany: Springer-Verlag, 185-212. - Jain, S.M. and Gupta, P.K. 2005. Protocol for Somatic Embryogenesis in Woody Plants, 497–504. Springer. Printed in the Netherlands. - Jimenez, V. M. and Bangerth, F. 2001. In vitro culture and endogenous hormone levels in immature zygotic embryos, endosperm and callus culture of normal and high-lysine barley genotypes. Journal of Applied Botany, 75: 1-7. - Joshi, R. and Kumar, P. 2013. Regulation of somatic embryogenesis in crops: a review. Agricultural Revolution, 34 (1): 1–20. - Khosravi, S., Azghandi, A.V., Hadad, R. and Mojtahedi, N. 2007. In vitro micrpropagation of Lilium longiflorum. Journal of Agricultural Research: Seed and Plant. 23: 159- 168. - Karkonen, A. 2001. Plant tissue culture as models for tree: somatic embryogenesis of Tilia cordata and lignin biosynthesis in Picea abies suspension cultures as case studies, division of plant physiology university of Helsinki. 75: 22-23. - Koroch, A.R., Kapteyn, J., Juliani, H.R. and Simon, J.E. 2002. In vitro regeneration and Agrobacterium transformation of Echinacea purpurea leaf explants. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. ASHS Press, Alexandria, pp 522–526 - Lakshmanan, P., Danesh, M. and Taji, A. 2002. Production of four commercially cultivated Echinacea species by different methods of in vitro regeneration. Journal of Horticultural Science Biotechnology, 77:158–163. - Larkin, P.J. and Scowcroft, W.R. 1981. Somaclonal variation a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics, 60: 197–214. - Li, N., Parson, B. L., Liu, D. R. and Mattoo, A. K. 1992. Accumulation of wound-inducible ACC syntheses transcript in tomato fruit is inhibited by salicylic acid and polyamines. Plant Molecular Biology, 18: 477-487. - Lindfors, A., Kuusela, H., Hohtola, A. and Kupila-Ahvenniemi, S. 1990. Molecular correlates of tissue browning and deterioration in scots pine calli. Biology Plant, 32:171-180. - Mahmood, A.J., Ebadi, A., Omidi, M. and Masoodi, M. 2013. "Effect of different concentrations of sucrose, casein hydrolysate and amino acids on somatic embryogenesis in some grapevine (Vitis vinifera)cultivars". Journal of Plant Production Research, 20 (1): 157-170. - Maria, O., Thomsen, M.O., Fretté, X.C., Christensen, K.B., Christensen, L.P, Grevsen, K. 2012. Seasonal variations in the concentrations of lipophilic compounds and phenolic acids in the roots of Echinacea purpurea and Echinacea pallid. Journal of Agriculture and Food Chemistry, 60:12131–12141. - Mauro, M. Cl., Nef, C. and Fallot, J. 1986. Simulation of somatic embryogenesis and plant regeneration from anther culture of Vitis vinifera cv. Cabernet-Sauvignon. Plant Cell Reports, 5: 377-380. - Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physio. 15: 473–497. - Murch, S. J., Peiris, S.E., Shi, W.L., Zobayed, S.M.A. and Saxena, P.K. 2006. Genetic diversity in seed populations of Echinacea purpurea controls the capacity for regeneration, route of morphogenesis and phytochemical composition. Plant Cell Reports, 25: 522-532. - Nutironchi, V., Caligo, M.A., Nozzolini, M. and Luccarini, G. 1984. Stimulation of carrot somatic embryogenesis by proline and serine. Plant Cell Reports, 3: 210-214. - OmidBeigi, R. 2010. Production and processing of medicinal plants, Volume 4, Astan Quds Razavi Printing and Publishing Institute, Mashhad. (In Persian) - Pareek, L. K. 2005. Trends in plant tissue culture and biotechnology. Agro Botanical Publishers, Nagar Bikaner. - Perry, N.B., Burgess, E.J. and Glennie, V.L. 2001. Echinacea standardization: analytical methods for phenolic compounds and typical levels in medicinal species. Journal of Agriculture and Food Chemistry, 49 (4): 1702–1706. - Peyvandi, M., Farahzadi, H., Arbabiyan, S., Hoseynimazinani, M. 2010. Effects of medium on somatic embryogenesis of Olea europea L. (cv. Kroneiki). Iranian Journal of Rangeland and plant breeding and plant research, 18(1), 93-101. (In Persian). - Pola, S.R. and Sarada, M.N. 2006. Somatic embryogenesis and plantlet regeneration in Sorghum bicolor (L.) Moench, from leaf segments. Journal of Cell and Molecular Biology, 5: 99-107. - Rezaii Gangeh, S., Dehestani-Ardakani, M., Kamali aliabad, K. 2019. The effect of different factors on somatic embryogenesis of stevia plant (Stevia rebaudiana Bertoni.). Iranian Journal of Rangeland and plant breeding and plant research, 27(1): 108-119. (In Persian). - Sadeghi, H., Khavari-Nejad, R., Fahimi, H. and Falahian, F. 2006. Effect of growth regulators on calli production and callus growth in various pieces of stem tissue culture of Tehran pine (Pinus eldarica M.). Journal of Agricultural Sciences & Natural Resources. 13 (4): 128-141. - Sanjabi, M. 2016. Induction of direct somatic embryogenesis in leaf explants of Ulmus glabra. Journal of Plant Researches, 28(4): 885-894. - Sharp, W.R., Sondhal, M.R. Caldar, R.S, Maraffa, S.B. 1986. The physiology of in vitro asexual embryogenesis. Hor. Rev., 2: 268–310. - Skoog, F.; Miller, C. O. 1957. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposia of the Society for Experimental Biology, 11:118–140. - Stanisavijevic, I., Stojicevic, S., Velickovic, D., Veljkovic, V. and Lazic, M. 2009. Antioxidant and antimicrobial activities of Echinacea (Echinacea purpurea L.) extracts obtained by classical and ultrasound extraction. Biotechnology and Bioengineering, Bioeng, 17: 478-483. - Steward, F.C., Mapes, M.O., Kent, A.E. and Holsten, R.D. 1964. Growth and development of cultured plant cells. Biochemical and morphogenic studies with cells yield new evidence on their metabolism and totipotency. Science, 143:20–27. - Thom, M., Maretzki, A., Komor, E. and Sakai, W.S. 1981. Nutrient uptake and accumulation by sugarcane cell cultures in relation to the growth cycle, Plant Cell Tissue and Organ Culture, 1: 3-14. - Wang, H.M., To, K.Y. 2004. Agrobacterium-mediated transformation in the high-value medicinal plant Echinacea purpurea. Plant Science, 166: 1087–1096. - White, A.R., Elmore, H.W., Watson, M.B. and Gill, J.P. 1989. Purification and partial characterization of polysaccharides from coconut milk. Annals of Botany, 64: 205-209. - Zamani, E., Dehestani-Ardakani, M., Kamali, K. (2020). Determination of the best culture medium for micropropagation of old Abarkooh cypress plant (Cupressus semprvirens var. horizontalis), Iranian Journal of Rangeland and plant breeding and plant research, 27(2): 240-251. (In Persian). - Zobayed, S.M.A. and Saxena, P.K. 2003. In vitro regeneration of Echinacea purpurea L.: enhancement of somatic embryogenesis by indolebutyric acid and dark pre-incubation. In Vitro Cellular & Developmental Biology-Plant, 39(6): 605-612. | ||
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