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رویانزایی بدنی گیاه دارویی سرخارگل (Echinacea purpurea (L.) Moench) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 8، دوره 28، شماره 1 - شماره پیاپی 55، شهریور 1399، صفحه 91-101 اصل مقاله (847.56 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2020.342034.1360 | ||
| نویسندگان | ||
| محدثه حجازی1؛ مریم دهستانی اردکانی* 2؛ کاظم کمالی3؛ محسن رشیدی4 | ||
| 1دانش آموخته کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان | ||
| 2استادیار گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه اردکان، یزد، ایران | ||
| 3دانشیار، گروه علوم خاک‌شناسی، دانشکده کشاورزی و منابع طبیعی، دانشگاه یزد | ||
| 4دانش آموخته کارشناسی ارشد، گروه علوم جانوری، دانشکده پیرادامپزشکی، دانشگاه اردکان | ||
| چکیده | ||
| سرخارگل (Echinacea purpurea (L.) Moench) یک گونه گیاهی با ارزش دارویی بالا میباشد. بهمنظور بهینهسازی پروتکل رویانزایی بدنی ریزنمونههای برگی گیاه سرخارگل از 18 تیمار ترکیبی شامل تنظیم کنندههای رشد شامل بنزیل آدنین (BA) در سه سطح (3، 4، 5 میلیگرم در لیتر)، نفتالین استیک اسید (NAA) در سه سطح (1/0، 2/0 و 5/0 میلیگرم در لیتر)، همراه و یا بدون یک گرم در لیتر زغال فعال، 50 میلیلیتر در لیتر شیره نارگیل و 50 میلیگرم در لیتر کازئینهیدرولیزات در محیط کشت MS استفاده شد. داده‌های بدست‌آمده در قالب طرح کاملا تصادفی در سه تکرار مورد تجزیه واریانس قرار گرفتند و مقایسه میانگین تیمارها به روش توکی انجام شد. نتایج نشان داد که رویانزایی با تولید کالوس بهصورت غیرمستقیم اتفاق افتاد. کالوسهای رویانزا به رنگ سبز تیره، منسجم و با رشد سریعتر بودند. بیشترین رویانزایی کالوسها (100 درصد) و نیز باززایی رویان در تیمارهای ترکیب BA و NAA همراه با زغال فعال، شیره نارگیل و کازئین هیدرولیزات حاصل شد. در حالیکه تیمارهای تنظیم‌کننده رشد فاقد شیره نارگیل و کازئین هیدرولیزات موفق به تولید رویان بدنی نشدند. بیشترین میزان تولید شاخساره (3/43 عدد) در تیمار ترکیبی 3 میلیگرم در لیتر BA+1/0 میلیگرم در لیتر NAA+1 میلیگرم در لیتر زغال فعال+50 میلیلیتر در لیتر شیره نارگیل+50 میلیگرم در لیتر کازئینهیدرولیزات مشاهده شد. بهطورکلی برای رویانزایی بدنی سرخارگل، استفاده از سطوح بالای BA و مقادیر اندک NAA به همراه شیرهنارگیل و کازئینهیدرولیزات توصیه میگردد. | ||
| کلیدواژه‌ها | ||
| بنزیل آدنین؛ شیره نارگیل؛ کازئین هیدرولیزات؛ کالوس؛ نفتالین استیک اسید | ||
| عنوان مقاله [English] | ||
| Somatic embryogenesis of medicinal plant of purple cone flower (Echinacea purpurea (L.) Moench) | ||
| نویسندگان [English] | ||
| Mohadeseh Hejazi1؛ Maryam Dehestani-Ardakani2؛ Kazem Kamali aliabad3؛ Mohsen Rashidi4 | ||
| 1M.Sc. Graduated, Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, I.R. Iran | ||
| 2Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran | ||
| 3Assoc. Prof., Department of Soil Science, Faculty of Agriculture and Natural Resources, Yazd University, Yazd, I.R. Iran. | ||
| 4M.Sc. Graduated, Department of Animal Sciences, Faculty of Pediatrics, Ardakan University, Ardakan, I.R. Iran. | ||
| چکیده [English] | ||
| Purple coneflower (Echinacea purpurea (L.) Moench) is a plant species of high medical value. Somatic embryogenesis is considered to be the most efficient micropropagation technique. Considering the importance of this plant, the current study has been performed with the aim of optimizing somatic embryogenic of that. Embryogenic callus induction and embryogenesis were investigated using different concentrations of plant growth regulations including Benzyl adenine (BA) in three levels (3, 4 and 5 mg/l), Naphtalene Acetic Acid (NAA) at three levels (0.1, 0.2 and 0.5 mg/l) with or without 1 g/l active charchoal, 50 ml/l coconut milkand 50 mg/l casein hydrolysate in leaf explants in MS culture medium in a completely randomized design with three replications. Induction of embryogenesis occurred indirectly with callus formation. Embryogenic calli were dark green, coherent with faster growth. The highest percentage of embryogenic callus (100 %) and also regeneration of callus (plantlents) were obtained in combination of BA and NAA with active charcoal, coconut milk and casein hydrolysate treatmens. Whereas the combination treatments of plant growth regulators without coconut milk and casein hydrolysate failed to produce somatic embryos. The maximum number of shoots (43.3 shoots) was observed in 3 mg/l BA + 0.1 mg/l NAA + 1 mg/l activate charcoal + 50 ml/l coconut milk + 50 mg/l casein hydrolysate treatment. | ||
| کلیدواژه‌ها [English] | ||
| Benzyl adenine, Coconut milk, Casein hydrolysate, Naphtalene Acetic Acid | ||
| مراجع | ||
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