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اثر عوامل محیطی دما و pH بر روی میزان ترکیب‌های بیوشیمیایی ریزجلبک Monoraphidium sp. | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 37، شماره 2 - شماره پیاپی 106، خرداد و تیر 1400، صفحه 290-299 اصل مقاله (657.93 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.343297.2808 | ||
| نویسندگان | ||
| پریچهر حناچی* 1؛ آسیه آقابابائی2؛ مصطفی نوروزی1 | ||
| 1دانشیار، گروه بیوتکنولوژی، بخش بیوشیمی، دانشکده علوم زیستی، دانشگاه الزهرا، تهران، ایران | ||
| 2کارشناسی ارشد، گروه بیوتکنولوژی، دانشکده علوم زیستی، دانشگاه الزهرا، تهران، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1400.37.290.106.2.1575.46 تولید متابولیت‌های ثانویه تحت شرایط محیطی مختلف تغییر می‌کند. هدف این مطالعه بررسی اثرات pH و دما بر روی محتوی ترکیب‌های آنتی‌اکسیدانی در گونه‌ای از جلبک‌های میکروسکوپی بود. نخست سویه ریز جلبکی Monoraphidium sp. تحت تیمارهای مختلف دمایی و pH کشت داده شد. سپس سنجش‌های بیوشیمیایی بر روی ترکیب‌هایی مانند میزان رنگریزه‌های فتوسنتزی، ترکیب‌های فنلی و فلاونوئید کل انجام شدند. فعالیت آنتی‌اکسیدانی کل به روش FRAP و DPPH مورد بررسی قرار گرفت. نتایج این تحقیق نشان داد که ریز جلبک Monoraphidium sp. در pH اسیدی و دمای 28 درجه سانتی‌گراد دارای حداکثر رشد و محتوای بالایی از ترکیب‌های آنتی‌اکسیدانی بود. نتایج حاصل بیانگر اینست که با تغییر عوامل محیطی می‌توان به شرایط اپتیمم برای تولید بالای ترکیب‌های آنتی‌اکسیدانی در ریز جلبک‌ها دست یافت که در صنایع دارویی، پزشکی و تولید مکمل‌های غذایی می‌توان از آن بهره گرفت. | ||
| کلیدواژه‌ها | ||
| ریزجلبک؛ Monoraphidium؛ فعالیت آنتی‌اکسیدانی؛ فنل؛ فلاونوئید | ||
| عنوان مقاله [English] | ||
| Effects of temperature and pH on biochemicals content of microalgae Monoraphidium sp. | ||
| نویسندگان [English] | ||
| P. Hanachi1؛ A. Aghababaie2؛ M. Noroozi1 | ||
| 1Biotechnology Department, Faculty of Biological Science, Alzahra University, Tehran, Iran | ||
| 2Biotechnology Department, Faculty of Biological Science, Alzahra University, Tehran, Iran | ||
| چکیده [English] | ||
| The production of secondary metabolites varies under the different environmental conditions. The aim of this study was to investigate the effects of pH and temperature on the content of antioxidant compounds in a species of microscopic algae. First, the microalgal strain Monoraphidium sp. was cultured under the different temperature and pH treatments. The biochemical assays were then performed on the compounds such as photosynthetic pigments, phenolic compounds, and total flavonoids. The total antioxidant activity was evaluated by FRAP and DPPH methods. The results of this study showed that the microalgae Monoraphidium sp. had the maximum growth and high content of antioxidant compounds at the acidic pH and temperature 28°C. The results indicated that the optimal conditions can be achieved for the high production of antioxidant compounds in microalgae by changing the environmental factors, which can be used in the pharmaceutical, medical, and production of nutritional supplements industries. | ||
| کلیدواژه‌ها [English] | ||
| Microalgae, Monoraphidium, antioxidant activity, phenol, flavonoid | ||
| مراجع | ||
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