| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,873 |
| تعداد مقالات | 32,555 |
| تعداد مشاهده مقاله | 42,417,623 |
| تعداد دریافت فایل اصل مقاله | 19,193,648 |
بررسی خاصیت ضد‌باکتریایی نانوذرات نقره بیوسنتز شده با استفاده از عصاره برگ زوفا (Hyssopus officinalis L.) تحت تأثیر تنش شوری | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 13، دوره 36، شماره 4 - شماره پیاپی 102، مهر و آبان 1399، صفحه 691-708 اصل مقاله (1.31 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128037.2651 | ||
| نویسندگان | ||
| ایمان رسایی1؛ مریم قنادنیا* 2؛ سعید باغشاهی3 | ||
| 1کارشناسی ارشد بیوتکنولوژی کشاورزی، گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| 2استادیار، گروه مهندسی علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| 3استاد، گروه مهندسی مواد، دانشکده فنی و مهندسی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1399.36.691.102.4.1576.1610 پیشرفت‌های اخیر در علوم زیست‌شناسی به‌دلیل پایه‌ای بودن آنها برای تعدادی از علوم مرتبط مانند کشاورزی، پزشکی، داروسازی، بیوتکنولوژی و حتی بیونانوتکنولوژی اهمیت ویژه‌ای یافته است. در این تحقیق تأثیر تیمارهای مختلف شوری (0، 50، 100 و 150 میکرومولار) بر گیاه زوفا (Hyssopus officinalis L.) و ویژگی‌های نانوذرات نقره (Ag NPs) بیوسنتز شده توسط عصاره این گیاه بررسی شد. تغییر رنگ محلول‌ها، وجود پیک در ناحیه nm 450 اسپکتروفتومتری و نتایج حاصل از تفرق اشعه ایکس (XRD)، بیوسنتز نانوذرات نقره را تأیید کرد. تصاویر مربوط به میکروسکوپ الکترونی روبشی نشر میدانی (FESEM) بیشتر ذرات را کروی شکل نشان داد که اشکال زاویه‌دار نیز در تیمارهای 50 و 100 میکرومولار قابل مشاهده بودند. نتایج حاصل از دستگاه تبدیل طیف‌سنجی مادون‌قرمز (FTIR) عوامل شیمیایی مختلف مانند OH، CO، =CH و C=C را که در فرایند بیوسنتز شرکت کرده بودند نشان داد. غلظت 50 میکرومولار از نمک بیشترین تأثیر را بر افزایش متابولیت‌های گیاهان داشت. کوچکترین نانوذرات (nm25.3) مربوط به تیمار شاهد (کروی شکل) بود. برخی نانوذرات بیوسنتز شده توسط غلظت 150 میکرومولار با اندازه nm 34.2، زاویه‌دار بوده و بیشترین خاصیت ضد‌باکتریایی را نشان دادند. نانوذرات نقره بیوسنتز شده، تأثیر منفی بیشتری بر باکتری‌های گرم منفی نسبت به گرم مثبت داشتند. بیشترین خاصیت ضد‌باکتریایی را نانوذرات بیوسنتز شده توسط غلظت 150 میکرومولار نشان دادند. این نتایج در ادامه تحقیقات قبلی ما برای اولین بار تأثیر تیمارهای شوری را بر ویژگی‌های نانوذرات بیوسنتز شده توسط عصاره گیاه زوفا نشان داد. این نتایج می‌توانند زمینه جالب توجهی برای بیوسنتز نانوذرات نقره ایجاد کنند که بتوانند جایگزین مناسبی برای آنتی‌بیوتیک‌ها باشند. | ||
| کلیدواژه‌ها | ||
| زوفا (Hyssopus officinalis L.)؛ زیست‌شناسی؛ نانو ذرات نقره؛ NaCl | ||
| عنوان مقاله [English] | ||
| Assesment of antibacterial properties of silver nanoparticles biosynthesized using leaf extract of Hyssopus officinalis L. grown under salinity stress | ||
| نویسندگان [English] | ||
| I. Rasaee1؛ M. Ghannadnia2؛ S. Baghshahi3 | ||
| 1Department of Biotechnology, Imam Khomeini International University, Qazvin, Iran | ||
| 2Department of Horticultural Sciences Engineering, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran | ||
| 3Department of Materials Engineering, Imam Khomeini International University, Qazvin, Iran | ||
| چکیده [English] | ||
| Recent advances in the biological sciences have become particularly important because they are the basis for some related sciences such as agriculture, medicine, pharmacology, biotechnology, and even bionanotechnology. In this study, the effect of different salinity treatments (0, 50, 100, and 150 µM) on Hyssopus officinalis L. and the properties of silver nanoparticles) Ag NPs) biosynthesized using these under-salinity stress plants leaves extract were investigated. The color change of the solutions, surface plasmon resonance at 450 nm and X-ray diffraction pattern confirmed the biosynthesis of Ag NPs. Field emission scanning electron microscopy (FESEM) images showed that most of the nanoparticles were spherical, with few angular shapes visible in 50 and 100 µM treatments. Fourier-transform infrared spectroscopy (FTIR) results revealed the participant functional groups of the plant extract in the biosynthesis process such as OH, CO, =CH and C=C. The 50 µM salinity treatment had the highest effect on increasing plant metabolites. The smallest nanoparticles (25.3 nm and spherical) were related to the control treatment. Some nanoparticles biosynthesized using the extract obtained from 150 µM salinity treatment were angular in shape with 34.2 nm in size and showed the highest antibacterial properties. Gram-negative bacteria were more sensitive to Ag NPs than the gram-positive ones. These results, following our previous research, revealed for the first time the effect of salinity treatments on the properties of Ag NPs biosynthesized using hyssop extract. The present results can provide an interesting background for Ag NPs biosynthesis that can be a good alternative to antibiotics. | ||
| کلیدواژه‌ها [English] | ||
| Hyssopus officinalis L, biology, silver nanoparticles, NaCl | ||
| مراجع | ||
|
- Abdel-Aziz, M.S., Shaheen, M.S., El-Nekeety, A.A. and Abdel-Wahhab, M.A., 2014. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. Journal of Saudi Chemical Society, 18: 356-363. - Afshar, P. and Sedaghat, S., 2016. Bio-Synthesis of silver nanoparticles using water extract of Satureja hortensis L. and evaluation of the antibacterial properties. Current Nanoscience, 12: 90-93. - Ajitha, B., Reddy, Y.A.K. and Reddy, P.S., 2014. Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their inborn antimicrobial activity. Spectrochimica Acta, 121: 164-172. - Arumugam, N., Thulasinathan, B., Pasubathi, R., Thangavel, K., Muthuramalingam, J.B. and Arunachalam., A., 2017. Biogenesis of silver nanoparticles using selected plant leaf extract; characterization and comparative analysis of their antimicrobial activity. Nanomedicine Journal, 4: 208-217. - Asharani, P., Handi, M.P. and Valiyaveettil, S., 2009. Anti-proliliferative activity of silver nanoparticles. BMC Molecular and Cell Biology, 10: 1-14. - Azza, A., El-Din, E., Eman, E., Azziz, S.F., Hendawy, A. and Omer, EA., 2009. Response of Thymus vulgaris L. to salt stress and alar (B9) in newly reclaimed soil. Journal of Applied Sciences Research, 5: 2165-2170. - Biener, J., Wittstock, A., Baumann, T.F., Welssmuller, J., Baumer, M. and Hamza, A.V., 2009. Surface chemistry in nanoscale materials. Materials, 2: 2404-2428. - Boisselier, E. and Astruc, D., 2009. Gold nanoparticles in nanomedicine: Preparations, imaging, diagnostics, therapies and toxicity. Chemical Society Reviews, 38: 1759-1782. - Behera, S., Ojha, A., Rout, J. and Nayak, P., 2012. Plant mediated synthesis of silver nanoparticles: Opportunity and challenge. International Journal of Biology Pharmacy and Allied Sciences, 1: - Boogar, R., 2014. Antibacterial effects of silver nanoparticles produced by Satureja hortensis extract on isolated Bacillus cereus from soil of sistan plain. International Journal of Infections Diseases, 1: 1-1. - Chang, T.Y., Chen, C.C., Cheng, K.M., Chin, C.Y., Chen, Y.H., Chen, X.A., Sun, J.R., Young, J.J. and Chiueh, T.T., 2017. Trimethyl chitosan-capped silver nanoparticles with positive surface charge: Their catalytic activity and antibacterial spectrum including multidrug-resistant strains of Acinetobacter baumannii. Colloid Surface, 155: 61-70. - Das, M. and Smita, S.S., 2018. Biosynthesis of silver nanoparticles using bark extracts of Butea monosperma (Lam.) Taub. and study of their antimicrobial activity. Applied Nanoscience, 8: 1059-1067. - Fathiazad, F. and Hamedeyazdan S., 2011. A review on Hyssopus officinalis L.: Composition and biological activities. African Journal of Pharmacy and Pharmacology, 5: 1959-1966. - Gilaki, M., 2010. Biosynthesis of silver nanoparticles using plant extracts. International Journal of Biological Sciences, 10: 465-467. - Gnanadesigan, M., Anand, M., Ravikumar, S., Maruthupandy, M., Vijayakumar, V., Selvam, S., Dhineshkumar, M. and Kumaraguru, A., 2011. Biosynthesis of silver nanoparticles by using mangrove plant extract and their potential mosquito larvicidal property. Asian Pacific Journal of Tropical Medicine, 4: 799-803. - Govindaraju, K., Tamilselvan, S., Kiruthing, V. and Singaravelu, G., 2010. Biogenic silver nanoparticles by Solanum torvum and their promising antimicrobial activity. Journal of Biopesticides, 3: 394-399. - Hajipour, M.J., Fromm, K.M., Ashkarran, A.A., Aberasturi, D.J., Larramendi, I.R., Rojo. T., Serpooshan, V., Parak, W.J. and Mahmoudi, M., 2012. Antibacterial properties of nanoparticles. Trends in Biotechnology, 30: 499-511. - Hoang, T.M., Moghaddam, L., Williams, B., Khanna, H., Dale, J. and Mundree, S.G., 2015. Development of salinity tolerance in rice by constitutive overexpression of genes involved in the regulation of programmed cell death. Frontiers in Plant Science, 6: 1-14. - Karray-Bouraoui, N., Rabhi, M., Neffati, M., Baldan, B., Ranieri, A., Marzouk, B., Lachaâl, M. and Smaoui, A., 2009. Salt effect on yield and composition of shoot essential oil and trichome morphology and density on leaves of Mentha pulegium. Industrial Crops and Products, 30: 338-343. - Kazazi, H., Rezaei, K., Ghotb-Sharif, S.J., Emam-Djomeh, Z. and Yamini, Y., 2007. Supercritical fluid extraction of flavors and fragrances from Hyssopous officinalis L. cultivated in Iran. Food Chemistry, 105: 805-811. - Kedziora, A., Speruda, M., Krzyzewska, E., Rybka, J., Lukowiak, A. and Bugla-Ptoskonska, G., 2018. Similarities and differences between silver ions and silver in nanoforms as antibacterial agents. International Journal of Molecular Sciences, 19: 1-17. - Khandekar, S.V., Kulkharni, M. and Devarajan, P.V., 2014. Polyaspartic acid functionalized gold nanoparticles for tumor targeted doxorubicin delivery. Journal of Biomedical Nanotechnology, 10: 143-153. - Kizil, S., Hasimi, N., Tolan, V. and Karatas, H., 2010. Chemical composition, antimicrobial and antioxidant activities of hyssop (Hyssopus officinalis L.) essential oil. Notulae Botanicae Hori Agrobotanici Cluj-Napoca, 38: 99-103. - Lidon, F.C. and Teixerira, M.G., 2000. Rice tolerance to excess Mn: implication in the chloroplast lamella synthesis of a novel Mn protein. Plant Physiology and Biochemistry, 38: 969-978. - Malinsky, M.D., Kelly, K.L., Schatz, G.C. and Van Duyne, R.P., 2001. Chain length dependence and sensing capabilities of the localized surface plasmon resonance of silver nanoparticles chemically modified with alkanethiol self-assembled monolayers. Journal of the American Chemical Society, 123: 1471-1482. - Maliszewska, I. and Sadowski, Z., 2009. Synthesis and antibacterial activity of silver nanoparticles, Journal of Physics: Conference Series, 146: 1-6. - Nahar, K., Aziz, S., Bashar, M.S., Haque, M.D.A. and Al-Reza, S.M.D., 2020. Synthesis and characterization of Silver nanoparticles from Cinnamomum tamala leaf extract and its antibacterial potential. International Journal of Nano Dimension, 11: 88-98. - Negrao, S., Schmockel, S.M. and Tester, M., 2017. Evaluating physiological responses of plants to salinity stress. Annals of Botany, 119: 1-11. - Oliveira, H., Barros, A.S., Delgadillo, I., Coimbra, M.A. and Santos, C., 2009. Effects of fungus inoculation and salt stress on physiology and biochemistry of in vitro grapevines: emphasis on sugar composition changes by FT-IR analysis. Environmental and Experimental Botany, 65: 1-10. - Oves, M., Aslam, M., Rauf, M.A., Qayyum, S., Qari, H.A., Khan, M.S., Alam, M.Z., Tabrez, S., Pugazhendhi, A. and Ismail, I.M., 2018. Antimicrobial and anticancer activities of silver nanoparticles synthesized from the root hair extract of Phoenix dactylifera. Materials Science and Engineering: C, 89: 429-443. - Pavia, D.L., Lampman, G.M., Kriz, G.S. and Vyvyan, J.A., 2008. Introduction to Spectroscopy. Cengage Learning, 752p. - Pirtarighat, S., Ghannadnia, M. and Baghshahi, S., 2017. Antimicrobial effects of green synthesized silver nanoparticles using Melissa officinalis grown under in vitro condition. Nanomedicine, 4: 184-190. - Pirtarighat, S., Ghannadnia, M. and Baghshahi, S., 2019. Biosynthesis of silver nanoparticles using Ocimum basilicum cultured under controlled condition for bactericidal application. Materials Science and Engineering C, 98: 250-255. - Pugazhendhi, A., Edison, T.N.J.I., Karuppusamy, I. and Kathirvel, B., 2018. Inorganic nanoparticles: a potential cancer therapy for human welfare. International Journal of Pharmaceutics, 539: 104-111. - Ramakrishna, A. and Ravishankar, G.A., 2011. Influence of abiotic stress signals on secondary metabolites in plants. Journal of Plant Signaling and Behavior, 6: 1720-1731. - Rai, M.K., Deshmukh, S.D., Ingle A.P. and Gade, A.K., 2012. Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. Applied Microbiology, 112: 841-852. - Rajesh, W.R., Jaya, R.L., Niranjan, S.K., Vijay, D.M. and Sahebrao, B.K., 2009. Phytosynthesis of silver nanoparticle using Gliricidia sepium (Jacq.). Current Nanoscience, 5: 117-122. - Rakholiya, K. and Chanda, S., 2012. In vitro interaction of certain antimicrobial agents in combination with plant extracts against some pathogenic bacterial strains. Asian Pacific Journal of Tropical Biomedicine, 2: 876-880. - Rasaee, I., Ghannadnia, M. and Baghshahi, S., 2018. Biosynthesis of silver nanoparticles using leaf extract of Satureja hortensis treated with NaCl and its antibacterial properties. Microporous and Mesoporous Materials, 264: 240-247. - Ravikumar, S., Gnanadesigan, M., Suganthi, P. and Ramalakshmi, A., 2010. Antibacterial potential of chosen mangrove plants against isolated urinary tract infectious bacterial pathogens. International Journal of Medical Sciences, 2: 94-99. - Roopan, S.M., Madhumitha, G., Rahuman, A.A., Kamaraj, C., Bharathi, A. and Surendra, T., 2013. Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Industrial Crops and Products, 43: 631-635. - Sanchez-Lopez, L., Gomes, D., Esteruelas, G., Bonilla, L., Lopez-Machado, A.L., Galindo, R., Cano, A., Espina, M., Ettecheto, M., Camins, A., Silva, A.M., Durazzo, A., Santini, A., Garcia, M.L. and Souto, E.B., 2020. Metal-based nanoparticles as antimicrobial agents: an overview. Nanomaterials, 10: 1-39. - Smith, A., Johnson, H. and Hall, M., 2003. Metabolic fingerprinting of salt-stressed tomatoes. Bulgarian Journal of Plant Physiology, 1: 153-163. - Sre, P.R., Reka, M., Poovazhagi, R., Kumar, M.A. and Murugesan, K., 2015. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica Lam. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 135: 1137-1144. - Stehfest, K., Boese, M., Kerns, G., Piry, A. and Wilhelm, C., 2004. Fourier transform infrared spectroscopy as a new tool to determine rosmarinic acid in situ. Journal of Plant Physiology, 161: 151-165. - Stehfest, K., Toepel, J. and Wilhelm, C., 2005. The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae. Plant Physiology and Biochemistry, 43: 717-726. - Taarit, M.B., Msaada, K., Hosni, K. and Marzouk, B., 2010. Changes in fatty acid and essential oil composition of sage (Salvia officinalis L.) leaves under NaCl stress. Food Chemistry, 119: 951-956. - Tahir, M., Khushtar, M., Fahad, M. and Rahman, M.D.A., 2018. Phytochemistry and pharmacological profile of traditionally used medicinal plant Hyssop (Hyssopus officinalis L.). Journal of Applied Pharmaceutical Science, 8: 132-140. - Umashankari, J., Inbakandan, D., Ajithkumar, T.T. and Balasubramanian, T., 2012. Mangrove plant, Rhizophora mucronata L. mediated one pot green synthesis of silver nanoparticles and its antibacterial activity against aquatic pthogens. Aquatic Biosynthesis, 8: 1-8. - Vanaja, M., Gnanajobitha, G., Paulkumar, K., Rajeshkumar, S., Malarkodi, C. and Annadurai, G., 2013. Phytosynthesis of silver nanoparticles by Cissus quadrangularis: Influence of physicochemical factors. Nanostructure in Chemistry, 3: 1-8. - Zargar, M., Hamid, A.A., Bakar, F.A., Shamsudin, M.N., Shameli, K., Jahanshiri, F. and Farahani F., 2011. Green synthesis and antibacterial effect of silver nanoparticles using Vitex negundo L. Molecules, 16: 6667-6676. - Zheljazkov, V.D., Astatkie, T. and Histov, A.N., 2012. Lavender and hyssop productivity, oil content, and bioactivity as a function of harvest time and drying. Industrial Crops and Products, 36: 222-228. | ||
|
آمار تعداد مشاهده مقاله: 525 تعداد دریافت فایل اصل مقاله: 401 |
||