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امکانسنجی نگهداری بذرهای شبخسب (Albizia julibrissin Durazz) در شرایط فراسرد | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 7، دوره 27، شماره 1 - شماره پیاپی 53، شهریور 1398، صفحه 58-71 اصل مقاله (718.05 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.120259 | ||
| نویسندگان | ||
| صالح سخاوت1؛ امید اسماعیل زاده* 2؛ مریم جبلی3 | ||
| 1دانشآموخته کارشناسی ارشد جنگلداری، دانشکده منابع طبیعی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2عضو هیات علمی گروه جنگلداری ، دانشگاه تربیت مدرس | ||
| 3کارشناس ارشد پژوهشی، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1398.27.73.53.1.1575.1583 شب خسب یکی از گونه های درختی نیامدار بومی جنگلهای جلگه ای و پایین بند ناحیه هیرکانی است که بهدلیل ابتلا به بیماری قارچی دچار صدمات زیادی شده است. در این تحقیق، امکان استفاده از فناوری فراسرد برای ذخیره سازی بذرهای شب خسب مورد بررسی قرار گرفت. جمعآوری بذر از تعداد 10 پایه درختی از جنگلهای پایینبند حوزه غرب هراز واقع در استان مازندران انجام شد. بهمنظور امکان سنجی قابلیت نگهداری بذرهای شب خسب در شرایط فراسرد از نتایج خصوصیات جوانه زنی بذرهای شب خسب در دو حالت نگهداری در شرایط فراسرد (196- درجه سانتیگراد) با چهار پیش تیمار آماده سازی شامل: ویتریفیکاسیون، گلیسرول 30 درصد، کاهش رطوبت و بدون پیش تیمار به همراه تیمار شاهد (نگهداری در شرایط خشک و دمای 15 درجه سانتیگراد) استفاده شد. بذرها پس از نگهداری یکهفته و یکماه در شرایط فراسرد خارج و در آب 42 درجه سانتیگراد (شوک حرارتی) قرار داده شدند. سپس برای جوانه زنی درون پتریدیش قرار گرفته و به ژرمیناتور با دمای 22+ درجه سانتیگراد منتقل گردیدند. ثبت و شمارش بذرهای جوانهزده بصورت روزانه تا زمانی که دیگر هیچ بذری جوانه نزد انجام شد. نتایج تحلیل واریانس دوطرفه و آزمون مقایسه میانگین دانکن نشان داد که صفات مختلف جوانهزنی بذرهای شبخسب در 4 پیشتیمار مربوط به فراسرد با تیمار شاهد تفاوت فاحشی نداشت. ازاینرو نتیجه گیری شد که نگهداری بذرهای شبخسب در شرایط فراسرد امکانپذیر است. نتایج این تحقیق همچنین تصریح می کند که بذرهای گونه شبخسب به دلیل داشتن پوسته سخت بذر و محتوای رطوبتی بسیار پایین می توانند بدون استفاده از مواد ضدانجماد (بدون پیش تیمار) در دمای 196- درجه سانتیگراد نگهداری شوند. ازاینرو میتوان بدون انجام پیش تیمار نسبت به ذخیره سازی بذرهای شبخسب در شرایط فراسرد اقدام نمود و بانک بذر فراسرد را بهعنوان راهبردی مؤثر برای حفاظت از ظرفیت ژنتیکی این گونه ارزشمند در سطح جنگلهای هیرکانی پیشنهاد کرد | ||
| کلیدواژهها | ||
| بانک بذر فراسرد؛ جوانهزنی بذر؛ شبخسب؛ کاهش رطوبت؛ گلیسرول؛ ویتریفیکاسیون | ||
| عنوان مقاله [English] | ||
| Feasibility of cryopreservation of Persian silk tree (Albizia julibrissin Durazz.) seeds | ||
| نویسندگان [English] | ||
| S. Sakhavat1؛ Omid Esmael zadeh2؛ M. Jebelli3 | ||
| 1M. Sc. Of forestry, Faculty of Natural Resources, tarbiat Modares University, Tehran, I.R.Iran. | ||
| 2, Assistant Professor of Forest Science and Engineering Department, Faculty of Natural Resources, tarbiat Modares University, Tehran, I.R. Iran | ||
| 3M. Sc. Biotechnology research group, Research Institute of Forests and Rangelands, Agricultural Research Education and Extention Organization (AREEO), Tehran, I.R. Iran. | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1398.27.73.53.1.1575.1583 Persian Silk tree (Albizia julibrissin Durazz.) is one of the native leguminous species in plain and lowland hyrcanian forests, which has been severely damaged by fungal disease. In this research, feasibility of preservation of A. julibrissin seeds in cryo condition was studied. Seeds of A. julibrissin were collected from 10 trees in lowland forests of western part of Haraz watershed, Mazandaran province, Iran. Seed germination characteristic of this species were analyzed in cryopreservation with four pretreatments including: vitrification solutions, 30% glycerol, desiccation and without cryoprotectants as well as control treatment, storage in dry condition at 15°C temperature. Seeds after one week and one month storage in liquid nitrogen condition were removed from the liquid nitrogen and the seeds were soaked into water at +42°C (heat shock). Then, seed were placed in petri dishes and so on were transferred into germinator at +22 ºC. Seed germination was daily checked as long as no seed has been germinated. Results of two way ANOVA followed by Duncan as a post hoc multiple comparisons showed that there were no significant differences between germination traits of A. julibrissin seeds in four pretreatments of cryopreservation with control treatment. So we concluded that cryopreservation of A. julibrissin seeds is feasible. Our result indicated that having hard seed coat and as well as very low moisture content enable A. julibrissin seeds to be preserved in cryo condition without any cryoprotectants. Therefore, cryopreservation of A. julibrissin seeds could be done without any pre-treatment and it could be proposed as an effective strategy to protect the genetic recourse of this valuable species of the Hyrcanian forests in Iran. | ||
| کلیدواژهها [English] | ||
| Germination, Seed, Albizia julibrissin, cryo seed bank, Glycerol, Vitrification solution, Desiccation | ||
| مراجع | ||
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