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بررسی تنوع ژنتیکی در برخی گونه‌های یولاف (.Avena spp) بومی ایران | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 4، دوره 27، شماره 1 - شماره پیاپی 53، شهریور 1398، صفحه 28-44 اصل مقاله (850.49 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.120253 | ||
| نویسندگان | ||
| شکیبا شاهمرادی* 1؛ مهدی زهراوی2؛ فرنگیس قنواتی3 | ||
| 1هیات علمی موسسه تحقیقات اصلاح و تهیه نهال و بذر کرج، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| 2استادیار،مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 3دانشیار،مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1398.27.28.53.1.1576.1606 در این تحقیق تعداد 49 نمونه ژنتیکی موجود در کلکسیون یولاف بانک ژن گیاهی ملی ایران، متعلق به چهار گونه Avena barbata، A. wiestii، A. fatua و A. sativa مورد بررسی قرار گرفتند. آزمایش به صورت مشاهده‌ای، در مزرعه پژوهشی مؤسسه تحقیقات اصلاح وتهیه نهال و بذر در کرج انجام شد. صفات آگرومورفولوژیکی مطابق با دستورالعمل مؤسسه ذخایر توارثی گیاهی بین‌المللی ارزیابی گردید. براساس مقادیر شاخص شانون، صفت رنگ لما دارای بالاترین مقدار تنوع (42/1) بود. در تجزیه به مؤلفه‌های اصلی، 43/68 درصد تغییرات موجود در داده‌ها، توسط هفت مؤلفه اصلی اول توجیه شد.دو نمونه ژنتیکی 439 (A. barbata) و 449 (A. barbata) دارای نزدیکترین و دو نمونه ژنتیکی 447 (A. wiestii) و 115059 (A.wiestii) دارای دورترین فاصله ژنتیکی بودند. نمونه‌های ژنتیکی مورد مطالعه با استفاده از تجزیه خوشه‌ای به روش K meansدر پنج گروه قرار داده شدند. براساس نتایج تجزیه تابع تشخیص، در مجموع سه تابع تشخیص حاصل گردید که به‌میزان 100 درصد از واریانس کل را شامل می‌شد. تابع اول بیشترین تمایز را بین دو گونه A. barbata و A.sativa، و تابع دوم بیشترین تمایز را بین گونه‌های A. fatuaو A. wiestii ایجاد نمود. مجموع نتایج این تحقیق بیانگر وجود تنوع مناسب در جمعیت‌های مورد بررسی از لحاظ صفات اندازه‌گیری شده بود که در امر به‌نژادی یولاف می‌تواند مورد استفاده قرار گیرد. همچنین از توابع تشخیص ایجاد شده در این تحقیق، می‌توان برای تمایز بین گونه‌های مربوطه استفاده کرد. | ||
| کلیدواژه‌ها | ||
| واژه‌های کلیدی:تابع تشخیص؛ ژرم‌پلاسم؛ شاخص شانون؛ نمونه ژنتیکی؛ یولاف | ||
| عنوان مقاله [English] | ||
| Evaluation of Genetic Diversity in some Oat species (Avena spp.)of Iran | ||
| نویسندگان [English] | ||
| shakiba shahmoradi1؛ M. Zahravi2؛ F. Ghanavati3 | ||
| 1Faculty member, Seed and Plant Improvement Institute/ Agriculture Research, Education and Extension Organization (AREEO) | ||
| 2Assist. Prof., Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R.Iran | ||
| 3Assoc. Prof., Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R.Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1398.27.28.53.1.1576.1606 In this research a total of 49 accessions in oat collection of National Plant Gene Bank of Iran belonging to four species of Avena barbata, A. wiestii, A. fatua and A. sativa were studied. The experiment was performed in an observatory designin research field of Seed and Plant Improvement Institute located in Karaj, Iran. The agromorphological traits were measured according to IBPGR descriptor. Based on Shannon index, lemma color had the largest variety (1.42). In principal component analysis, the first seven components comprised 68.43% of total variation. Two accessions of 439 (A. barbata) and 449 (A. barbata) were the nearest and two accessions of 447 (A. wiestii) and 115059 (A. wiestii) and were the farthest based on genetic distance. The studied accessions were placed in five groups by K means clustering method. Three discriminant functions were developed justifying total variance in data. The first and second discriminant functionsmaintained the most distinction between two species of A. barbata and A.sativa and between two species of A. fatua and A. wiestii, respectively. Total results indicated presence of suitable diversity in the studied population based on the measured traits which could be exploited in oat breeding in future. In addition, the discriminant functions developed in this research could be used in distinguishing the relating oat species | ||
| کلیدواژه‌ها [English] | ||
| Discriminant function, Germplasm, Accession, Shannon index, Oat | ||
| مراجع | ||
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