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تأثیر تنش خشکی بر برخی خصوصیات مورفوفیزیولوژیکی و فیتوشیمیایی مرزنجوش بخارایی (Origanum vulgare L. ssp. gracile) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 35، شماره 2 - شماره پیاپی 94، خرداد و تیر 1398، صفحه 252-265 اصل مقاله (608.64 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.123365.2400 | ||
| نویسندگان | ||
| آزاد مینائی1؛ عباس حسنی* 2؛ حسین ناظمیه3؛ سینا بشارت4 | ||
| 1دانشجوی دکتری، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3استاد، مرکز تحقیقات ریزفناوری دارویی، دانشکده داروسازی، دانشگاه علوم پزشکی تبریز، تبریز، ایران | ||
| 4دانشیار، گروه مهندسی آب، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.252.94.2.1578.41 به‌منظور بررسی اثر سطوح مختلف تنش خشکی بر رشد، عملکرد و برخی خصوصیات فیزیولوژیکی و فیتوشیمیایی گیاه مرزنجوش بخارایی (Origanum vulgare L. ssp. gracile)، آزمایشی گلدانی در قالب طرح کاملاً تصادفی با چهار تیمار و پنج تکرار انجام گردید. تیمارهای تنش خشکی شامل چهار سطح 100% (بدون تنش)، 80%، 60% و 40% ظرفیت زراعی بودند. نتایج نشان داد که تنش خشکی تأثیر معنی‌داری بر مؤلفه‌های رشدی، عملکرد ماده خشک، محتوی نسبی آب برگ (RWC)، فنل کل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی، کلروفیل a، b و کلروفیل کل و درصد و عملکرد اسانس دارد. از نظر مقدار کاروتنوئید اختلاف معنی داری بین تیمارهای مختلف وجود نداشت. با کاهش مقدار آب خاک، ارتفاع بوته، قطر ساقه، تعداد و طول شاخه‌های جانبی، عملکرد ماده خشک، RWC، کلروفیل a، b و کلروفیل کل و عملکرد اسانس کاهش و در مقابل میزان فنل و فلاونوئید کل، فعالیت آنتی‌اکسیدانی و درصد اسانس افزایش یافت. علیرغم کاهش عملکرد ماده خشک، اختلاف بین تیمارهای 80%، 60% و 40% ظرفیت زراعی معنی‌دار نبود. بیشترین درصد اسانس (54/1 میلی‌لیتر در صد گرم ماده خشک) در تیمار 60% ظرفیت زراعی و بیشترین مقدار فنل کل (21/5 میلی‌گرم گالیک اسید در گرم ماده تر)، فلاونوئید کل (5/0 میلی‌گرم کوئرستین در گرم ماده تر) و فعالیت آنتی‌اکسیدانی (36/77%) در تیمار 40% ظرفیت زراعی مشاهده شد. | ||
| کلیدواژه‌ها | ||
| تنش کم‌آبی؛ ترکیب‌های آنتی‌اکسیدانی؛ عملکرد؛ محتوای نسبی آب برگ؛ مرزنجوش (Origanum) | ||
| عنوان مقاله [English] | ||
| Effect of drought stress on some morphophysiological and phytochemical characteristics of oregano (Origanum vulgare L. ssp. gracile) | ||
| نویسندگان [English] | ||
| A. Minaei1؛ A. Hassani2؛ H. Nazemiyeh3؛ S. Besharat4 | ||
| 1Ph.D. student, Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 2Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Department of Water Engineering, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده [English] | ||
| To study the effect of different levels of drought stress on growth, yield and some physiological and phytochemical characteristics of oregano (Origanum vulgare L. ssp. gracile), a pot experiment, in a randomized complete design with four treatments and five replications, was conducted. Drought stress treatments were 100% (non-stress), 80%, 60% and 40% of field capacity. The results showed that drought stress had significant effect on growth parameters, dry herb yield, leaf relative water content (RWC), total phenol and flavonoid content, antioxidant activity, a, b and total chlorophyll content, essential oil content and yield. There was no significant difference between drought stress treatments for carotenoids. As the soil water content decreased, plant height, stem diameter, number and length of axillary shoots, dry herb yield, RWC, a, b and total chlorophyll content and essential oil yield decreased, but total phenol and flavonoid content, antioxidant activity and essential oil content increased. Despite the decrease in dry herb yield, the difference between 80%, 60% and 40% treatments was not significant. The highest essential oil content (1.54 ml/100 g DW) was observed in 60% of field capacity and the highest amounts of total phenol (5.21 mg gallic acid/g FW), total flavonoid (0.5 mg quercetin/g FW) and antioxidant activity (77.36%) were obtained in 40% of field capacity. The highest percentage of essential oil (54.1 mg / g dry matter) was observed in 60% of treatment capacity and the highest amount of total phenol (0.51 mg gallic acid per gram of fresh material), total flavonoid (0.5 mg Gram quercetin in grams of fresh milk) and antioxidant activity (37.36%) in 40% of treatment capacity. | ||
| کلیدواژه‌ها [English] | ||
| Water deficit stress, antioxidant compounds, yield, leaf relative water content, Oregano | ||
| مراجع | ||
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