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تأثیر تنش خشکی بر برخی خصوصیات مورفوفیزیولوژیکی و فیتوشیمیایی مرزنجوش بخارایی (Origanum vulgare L. ssp. gracile) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 35، شماره 2 - شماره پیاپی 94، خرداد و تیر 1398، صفحه 252-265 اصل مقاله (608.64 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.123365.2400 | ||
| نویسندگان | ||
| آزاد مینائی1؛ عباس حسنی* 2؛ حسین ناظمیه3؛ سینا بشارت4 | ||
| 1دانشجوی دکتری، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3استاد، مرکز تحقیقات ریزفناوری دارویی، دانشکده داروسازی، دانشگاه علوم پزشکی تبریز، تبریز، ایران | ||
| 4دانشیار، گروه مهندسی آب، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.252.94.2.1578.41 به‌منظور بررسی اثر سطوح مختلف تنش خشکی بر رشد، عملکرد و برخی خصوصیات فیزیولوژیکی و فیتوشیمیایی گیاه مرزنجوش بخارایی (Origanum vulgare L. ssp. gracile)، آزمایشی گلدانی در قالب طرح کاملاً تصادفی با چهار تیمار و پنج تکرار انجام گردید. تیمارهای تنش خشکی شامل چهار سطح 100% (بدون تنش)، 80%، 60% و 40% ظرفیت زراعی بودند. نتایج نشان داد که تنش خشکی تأثیر معنی‌داری بر مؤلفه‌های رشدی، عملکرد ماده خشک، محتوی نسبی آب برگ (RWC)، فنل کل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی، کلروفیل a، b و کلروفیل کل و درصد و عملکرد اسانس دارد. از نظر مقدار کاروتنوئید اختلاف معنی داری بین تیمارهای مختلف وجود نداشت. با کاهش مقدار آب خاک، ارتفاع بوته، قطر ساقه، تعداد و طول شاخه‌های جانبی، عملکرد ماده خشک، RWC، کلروفیل a، b و کلروفیل کل و عملکرد اسانس کاهش و در مقابل میزان فنل و فلاونوئید کل، فعالیت آنتی‌اکسیدانی و درصد اسانس افزایش یافت. علیرغم کاهش عملکرد ماده خشک، اختلاف بین تیمارهای 80%، 60% و 40% ظرفیت زراعی معنی‌دار نبود. بیشترین درصد اسانس (54/1 میلی‌لیتر در صد گرم ماده خشک) در تیمار 60% ظرفیت زراعی و بیشترین مقدار فنل کل (21/5 میلی‌گرم گالیک اسید در گرم ماده تر)، فلاونوئید کل (5/0 میلی‌گرم کوئرستین در گرم ماده تر) و فعالیت آنتی‌اکسیدانی (36/77%) در تیمار 40% ظرفیت زراعی مشاهده شد. | ||
| کلیدواژه‌ها | ||
| تنش کم‌آبی؛ ترکیب‌های آنتی‌اکسیدانی؛ عملکرد؛ محتوای نسبی آب برگ؛ مرزنجوش (Origanum) | ||
| عنوان مقاله [English] | ||
| Effect of drought stress on some morphophysiological and phytochemical characteristics of oregano (Origanum vulgare L. ssp. gracile) | ||
| نویسندگان [English] | ||
| A. Minaei1؛ A. Hassani2؛ H. Nazemiyeh3؛ S. Besharat4 | ||
| 1Ph.D. student, Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 2Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Department of Water Engineering, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده [English] | ||
| To study the effect of different levels of drought stress on growth, yield and some physiological and phytochemical characteristics of oregano (Origanum vulgare L. ssp. gracile), a pot experiment, in a randomized complete design with four treatments and five replications, was conducted. Drought stress treatments were 100% (non-stress), 80%, 60% and 40% of field capacity. The results showed that drought stress had significant effect on growth parameters, dry herb yield, leaf relative water content (RWC), total phenol and flavonoid content, antioxidant activity, a, b and total chlorophyll content, essential oil content and yield. There was no significant difference between drought stress treatments for carotenoids. As the soil water content decreased, plant height, stem diameter, number and length of axillary shoots, dry herb yield, RWC, a, b and total chlorophyll content and essential oil yield decreased, but total phenol and flavonoid content, antioxidant activity and essential oil content increased. Despite the decrease in dry herb yield, the difference between 80%, 60% and 40% treatments was not significant. The highest essential oil content (1.54 ml/100 g DW) was observed in 60% of field capacity and the highest amounts of total phenol (5.21 mg gallic acid/g FW), total flavonoid (0.5 mg quercetin/g FW) and antioxidant activity (77.36%) were obtained in 40% of field capacity. The highest percentage of essential oil (54.1 mg / g dry matter) was observed in 60% of treatment capacity and the highest amount of total phenol (0.51 mg gallic acid per gram of fresh material), total flavonoid (0.5 mg Gram quercetin in grams of fresh milk) and antioxidant activity (37.36%) in 40% of treatment capacity. | ||
| کلیدواژه‌ها [English] | ||
| Water deficit stress, antioxidant compounds, yield, leaf relative water content, Oregano | ||
| مراجع | ||
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- Agbo, M.O., Uzor. P.F., Nneji, U.N.A., Odurukwe, C.U.E., Ogbatue, U.B. and Mbaoji, E.C., 2015. Antioxidant, total phenolic and flavonoid content of selected Nigerian medicinal plants. Dhaka University Journal of Pharmaceutical Sciences, 14(1): 35-41. - Akhzari, D. and Pessarakli, M., 2016. Effect of drought stress on total protein, essential oil content and physiological traits of Levisticum officinale Koch. Journal of Plant Nutrition, 39(10): 1365-1371. - Albouchi, A., Bejaoui, Z. and El Aouni M.H., 2003. Influence of moderate or severe water stress on the growth of Casuarina glauca Sieb. seedlings. Sécheresse, 14(3): 137-142. - Andre, C.M., Schafleitner, R., Legay, S., Lefèvre, I., Aliaga, C.A., Nomberto, G., Hoffmann, L., Hausman, J.F., Larondelle, Y. and Evers, D., 2009. Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry, 70(9): 1107-1116. - Anjum, S.A., Xie, X.Y., Wang, L.C., Saleem, M.F., Man, C. and Lei, W., 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research, 6(9): 2026-2032. - Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23: 112-121. - Ashraf, M. and Foolad, M.R., 2007. Roles of glycine, betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59: 206-216. - Askari, E. and Ehsanzadeh, P., 2015. Osmoregulation-mediated differential responses of field-grown fennel genotypes to drought. Industrial Crops and Products, 76: 494-508. - Babaee, K., Amini Dehaghi, M., Modares Sanavi, S.A.M. and Jabbari, R., 2010. Water deficit effect on morphology, proline content and thymol percentage of Thyme (Thymus vulgaris L.). Iranian Journal of Medicinal and Aromatic Plants, 26(2): 239-251. - Bettaieb, I., Zakhama, N., Wannes, W.A., Kchouk, M.E. and Marzouk, B., 2009. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Scientica Horticulturae, 120(2): 271-275. - Bryant, J.P., Chapin, F.S.I. and Klein, D.R., 1983. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos, 40: 357-368. - Burits, M. and Bucar, F., 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14(5): 323-328. - Chalker-Scott, L., 2002. Do anthocyanins function as osmoregulator in leaf tissues?. Advances in Botanical Research, 37: 103-127. - Dashti, M., Kafi, M., Tavakkoli, H. and Mirza, M., 2015. Investigation on some morphophysiological indices in Salvia leriifolia Benth. under water deficit stress. Iranian Journal of Field Crops Research, 13(2): 298-307. - De Abreu, I.N. and Mazzafera, P., 2005. Effect of water and temperature stress on the content of constituents of Hypericum brasiliense choisy. Plant Physiology and Biochemistry, 43: 241-248. - Dixion, R.A. and Paiva, N.L., 1995. Stress-induced phenylpropanoid metabolism. Plant Cell, 7: 1085-1097. - Ebrahimzadeh, M.A., Hosseinimehr, S.J., Hamidian, A. and Jafari, M., 2008. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacologonline, 1: 7-14. - Foyer, C.H., Valadier, M.H., Migge, A. and Becker, T.W., 1998. Drought induced effects on reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leaves. Plant Physiology, 117: 283-292. - Gharibi, S., Tabatabaei, B.E., Saeidi, G. and Goli, S.A., 2016. Effect of drought stress on total phenolic, lipid peroxidation, and antioxidant activity of Achillea species. Applied Biochemistry and Biotechnology, 178(4): 796-809. - Hassani, A., 2006. Effect of water deficit stress on growth, yield and essential oil content ofDracocephalum moldavica. Iranian Journal of Medicinal and Aromatic Plants, 22(3): 256-261. - Hazzoumi, Z., Moustakime, Y., Elharchli, H. and Joutei, K.M., 2015. Effect of arbuscular mycorrhizal fungi (AMF) and water stress on growth, phenolic compounds, glandular hairs, and yield of essential oil in basil (Ocimum gratissimum L.). Chemical and Biological Technologies in Agriculture, 2: 1-11. - Irigoyen, J.J., Einerich, D.W. and Sánchez‐Díaz, M., 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84: 55-60. - Inze, D. and Montagu, M.V., 2000. Oxidative Stress in Plant. Tj International Ltd, Padstow, Cornawall, Great Britain, 321p. - Jaafar, H.Z.E., Ibrahim, M.H. and Mohamad Fakri, N.F., 2012. Impact of soil field water capacity on secondary metabolites, phenylalanine ammonia-lyase (PAL), malondialdehyde (MDA) and photosynthetic responses of Malaysian Kacip Fatimah (Labisia pumila Benth). Molecules, 17(6): 7305-7322. - Jaleel, C.A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H.J., Somasundaram, R. and Panneerselvam, R., 2009. Drought stress in plants: A review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology, 11: 100-105. - Jordan, M.J., Martinez, R.M., Cases, M.A. and Sotomayor, J.A., 2003. Watering level effect on Thymus hyemalis Lange essential oil yield and composition. Journal of Agricultural and Food Chemistry, 51(18): 5420-5427. - Kafi, M., Borzoee, A., Salehi, M., Kamandi, A., Masoumi, A. and Nabati, J., 2009. Physiology of Environmental Stresses in Plants. Jahad Daneshgahi Press, 502p. - Kokkini, S., 1997. Taxonomy, diversity and distribution of Origanum species. Proceeding of the IPGRI International Workshop on Oregano, CIHEAM, Valenzano, Bari, Italy, 8-12 May. - Lawlor, D.W. and Cornic, G., 2002. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environment, 25(2): 275-294. - Manukyan, A., 2011. Effect of growing factors on productivity and quality of lemon catmint, lemon balm and sage under soilless greenhouse production. I: Drought stress. Medicinal and Aromatic Plant Science and Biotechnology, 5: 119-125. - Maroofi, H., 2005. Final report of collection and identification of the flora of Kurdistan province and establishment of the provincial herbarium (phase 2), Research Institute of Forests and Rangelands, 249p. - Mohammadi, H., Esmailpour, M., Ghorbi, S. and Hatami, M., 2018. Physiological and biochemical changes in Matricaria chamomilla induced by Pseudomonas fluorescens and water deficit stress. Acta Agriculturae Slovenica, 111(1): 63-72. - Moradi, M., Hassani, A., Sefidkon, F. and Maroofi, H., 2015. Chemical composition of leaves and flowers essential oil of Origanum vulgare ssp. gracile growing wild in Iran. Journal of Essential Oil Bearing Plants, 18(1): 242-247. - Nasibi, F. and Kalantari, K.H. 2005. The effects of UV-a, UV-b and UV-c on protein and ascorbate content, lipid peroxidation and biosynthesis of screening compounds in Brassica napus. Iranian Journal of Science and Technology, 29(1): 39-48. - Oh, M.M., Trick, H.N. and Rajashekar, C.B., 2009. Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce. Journal of Plant Physiology, 166: 180-191. - Omidbaigi, R., 1997. Approaches to Production and Processing of Medicinal Plants (Vol 2). Tarrahane Nashr, Mashhad, Iran, 424p. - Omidbaigi, R., Hassani, A. and Sefidkon, F., 2003. Essential oil content and composition of sweet basil (Ocimum basilicum) at different irrigation regimes. Journal of Essential Oil Bearing Plants, 6(2): 104-108. - Radacsi, P., Inotai, K., Sarosi, S., Czovek, P., Bernath, J. and Nemeth, E., 2010. Effect of water supply on the physiological characteristics and production of basil (Ocimum basilicum L.). European Journal of Horticultural Science, 75: 193-197. - Ramakrishna, A. and Ravishankar, G.A., 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior, 6(11): 1720-1731. - Said-Al Ahl, H.A.H., Omer, E.A. and Naguib, N.Y., 2009. Effect of water stress and nitrogen fertilizer on herb and essential oil of oregano. International Agrophysics, 23(3): 269-275. - Selmar, D. and Kleinwächter, M., 2013. Influencing the product quality by deliberately applying drought stress during the cultivation of medicinal plants. Industrial Crops and Products, 42: 558-566. - Silva, E.N., Ribeiro, R.V., Ferreira-Silva, S.L., Viegas, R.A. and Silveira, J.A.G., 2010. Comparative effects of salinity and water stress on photosynthesis, water relations and growth of Jatropha curcas plants. Journal of Arid Environments, 74(10): 1130-1137. - Simon, J.E., Bubenheim, R.D., Joly, R.J. and Charles, D.J., 1992. Water stress-induced alterations in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4(1): 71-75. - Singh, A.K. and Dubey, R.S., 1995. Changes in chlorophyll a and b contents and activities of photosystems I and II in rice seedlings induced by NaCl. Photosynthetica, 31: 489-499. - Singh, M., Ganesha-Rao, R.S. and Ramesh, S., 1997. Irrigation and nitrogen requirement of lemongrass (Cymbopogon flexuosus (Sleud) Wats) on a red sandy loam soil under semiarid tropical conditions. Journal of Essential Oil Research, 9(5): 569-574. - Spada, P. and Perrino, P., 1996. Conservation of oregano species in national and international collections: an assessment. Proceedings of the IPGRI International Workshop on Oregano, CIHEAM, Valenzano, Bari, Italy, 8-12 May: 14-23. - Sotomayor, J.A., Martinez, R.M., Garcia, A.J. and Jordan, M.J., 2004. Thymus zygis subsp. gracilis: watering level effect on phytomass production and essential oil quality. Journal of Agricultural and Food Chemistry, 52(17): 5418-5424. - Tátrai, Z.A., Sanoubar, R., Pluhár, Z., Mancarella, S., Orsini, F. and Gianquinto, G., 2016. Morphological and physiological plant responses to drought stress in Thymus citriodorus. International Journal of Agronomy, 20(16): 1-8. - Turner, N.C., 1981. Techniques and experimental approaches for the measurement of plant water status. Plant and Soil, 58: 339-366. - Turtola, S., Manninen, A.M., Rikala, R. and Kainulainen, P., 2003. Drought stress alters the concentration of wood terpenoids in Scots pine and Norway spruce seedlings. Journal of Chemical Ecology, 29: 1981-1985. - Yildiz-Aktas, L., Dagnon, S., Gurel, A., Gesheva, E. and Edreva, A., 2009. Drought tolerance in cotton: Involvement of non-enzymatic ROS-scavenging compounds. Journal of Agronomy and Crop Science, 195(4): 247-253. | ||
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