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اثر استفاده از سطوح مختلف ساپونین بر قابلیت هضم مواد مغذی، فراسنجه‌های تخمیری، جمعیت پروتوزوآی شکمبه‌ای و فراسنجه‌های خونی در گوسفندان نر بلوچی | ||
| علوم دامی | ||
| مقاله 20، دوره 30، شماره 117، اسفند 1396، صفحه 241-256 اصل مقاله (1.53 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2018.116064 | ||
| نویسندگان | ||
| محمد مهدی محقی1؛ عبدالمنصور طهماسبی2؛ رضا ولی زاده2؛ عباسعلی ناصریان2؛ محسن کاظمی* 3؛ آمنه اسکندری تربقان4 | ||
| 1استادیار گروه علوم دامی دانشکده کشاورزی دانشگاه هرات افغانستان | ||
| 2استاد گروه علوم دامی دانشکده کشاورزی دانشگاه فردوسی مشهد | ||
| 3استادیار گروه علوم دامی مجتمع آموزش عالی تربت جام | ||
| 4مربی گروه مهندسی بهداشت محیط دانشکده علوم پزشکی تربت جام | ||
| چکیده | ||
| این آزمایش با هدف مطالعه اثر استفاده از سطوح مختلف ساپونین خالص(Loba Chemie Ltd.) بر قابلیت هضم، فراسنجه‌های تخمیری، جمعیت پروتوزوآی شکمبه‌ای و فراسنجه‌های خونی در گوسفندان نر بلوچی انجام شد. از 4 رأس گوسفند نر بلوچی با میانگین وزن زنده 2/2±3/40 کیلوگرم و دارای فیستولای شکمبه‌ای در قالب طرح چرخشی استفاده شد. تیمارهای آزمایشی شامل 1-جیره پایه (شاهد)، 2-جیره پایه + 75 میلی‌گرم ساپونین در هرکیلوگرم ماده خشک جیره، 3-جیره پایه + 150 میلی‌گرم ساپونین و 4-جیره پایه + 300 میلی‌گرم ساپونین بودند. ساپونین، تأثیری بر قابلیت هضم ظاهری ماده خشک، ماده آلی، پروتئین خام، چربی خام، الیاف نامحلول در شوینده اسیدی و خنثی (ADF وNDF) جیره نداشت (05/0P>). غلظت استات، پروپیونات، بوتیرات، والرات و نیز pH مایع شکمبه تحت تأثیر تیمارها قرار نگرفت (05/0P>)، اما سطح 300 میلی‌گرم ساپونین باعث افزایش کل اسیدهای چرب فرار در مقایسه با تیمار شاهد شد (05/0>P). با افزایش ساپونین در جیره، میانگین غلظت نیتروژن آمونیاکی و جمعیت پروتوزوآ کاهش یافت (05/0>P). همچنین میانگین سطح تری‌گلیسیرید خون با افزایش ساپونین در جیره، کاهش یافت (05/0>p) ولی سطح کلسترول، لیپوپروتئین‌های با چگالی کم یا زیاد (LDL و HDL) تحت تأثیر تیمارها قرار نگرفت (05/0P>). هرچند استفاده از ساپونین تا سطح 300 میلی‌گرم در جیره گوسفندان در کوتاه مدت، اثر سوئی بر سلامت دام نداشت، اما در میان فراسنجه‌های مورد مطالعه، غلظت نیتروژن آمونیاکی، کل اسیدهای چرب فرار، تری‌گلیسرید، و شمار پروتوزوآی شکمبه‌ای تحت تأثیر مصرف ساپونین قرار گرفت. با این وجود برای اثبات اثرات مفید ساپونین بر عملکرد دام، نیاز به مطالعات بیشتر می‌باشد. | ||
| کلیدواژه‌ها | ||
| ساپونین؛ گوسفند؛ تخمیر؛ پروتوزوآ؛ متابولیت‌های خونی | ||
| عنوان مقاله [English] | ||
| The effect of different levels of saponin on nutrient digestibility, fermentation parameters, ruminal protozoa population and blood metabolites in Baluchi male sheep | ||
| نویسندگان [English] | ||
| Mohammad Mehdi Moheghi1؛ Abdoul Mansour Tahmasbi2؛ Reza Valizadeh2؛ Abasali Naserian2؛ mohsen kazemi3؛ Ameneh Eskandary Torbaghan4 | ||
| 11Assistant professor, Department of Animal Science, College of Agriculture, University of Herat, Afghanistan | ||
| 23Professor, Department of Animal science, College of Agriculture, Ferdowsi University of Mashhad | ||
| 3Higher Education Complex of Torbat-e Jam | ||
| 44Professional engineering, Department of Environmental Health Engineering, Torbat-e Jam Faculty of Medical Sciences | ||
| چکیده [English] | ||
| This experiment was conducted to study the effect of different levels of pure saponin (Loba Chemie Ltd.) on digestibility, fermentation parameters, ruminal protozoa population and blood metabolites in Baluchi male sheep. Four rumen fistulated Baluchi male sheep (LW=40.3±2.2kg) were used in a change-over design. Experimental treatments were 1) basal diet (control), 2) basal diet + 75 mg saponin per kg of diet (DM basis), 3) basal diet + 150 mg saponin, and 4) basal diet + 300 mg saponin. Saponin did not affect apparent digestibility of dry matter, organic matter, crude protein, crude fat, NDF, and ADF of the diet (P>0.05). Concentration of acetate, propionate, butyrate, valerate and also pH of the rumen fluid were not affected by the treatments (P>0.05), but the level of 300 mg saponin increased total volatile fatty acids compared to the control group (P<0.05). The mean of ammonia nitrogen concentration and total number of protozoa were decreased with the increasing of saponin in the diet (P<0.05). Also by increasing saponin in the diet, the mean of blood triglyceride concentration decreased (P<0.05) but cholesterol, HDL, and LDL level were not affected by the treatments (P>0.05). Although use of saponin up to 300 mg in the diet, had no negative effect on the health of animal, but among the studied parameters, ammonia nitrogen concentration, total volatile fatty acids, blood triglyceride and numbers of ruminal protozoa were affected by saponin. However, more studies are needed to prove the beneficial effects of saponins on animal performance. | ||
| کلیدواژه‌ها [English] | ||
| saponin, Sheep, fermentation, protozoa, Blood metabolites | ||
| مراجع | ||
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