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ویژگیهای بیوشیمیایی پروتئازهای گوارشی کفشدوزک خربزه Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) | ||
| نامه انجمن حشره شناسی ایران | ||
| مقاله 1، دوره 37، شماره 3 - شماره پیاپی 70، آذر 1396، صفحه 293-304 اصل مقاله (285.38 K) | ||
| نوع مقاله: مقاله کامل، فارسی | ||
| شناسه دیجیتال (DOI): 10.22117/jesi.2017.113584 | ||
| نویسندگان | ||
| مولود غلام‌زاده چیتگر* 1؛ روح انگیز قنبری نژاد2؛ محمد قدمیاری3 | ||
| 1هیات علمی / مرکز تحقیقات کشاورزی مازندران- تحقیقات گیاهپزشکی تنکابن | ||
| 2دانشجوی سابق کارشناسی ارشد گروه گیاهپزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| 3گروه گیاهپزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| چکیده | ||
| کفشدوزک خربزه، (Col.:Coccinellidae)Epilachna chrysomelina (Fabricius) به عنوان یکی از مهمترین آفات گیاهان خانواده کدوئیان قادر است با تغذیه از برگ‌های گیاه در سنین لاروی و حشره کامل خسارت جدی به این محصولات وارد سازد. در تحقیق حاضر ویژگیهای بیوشیمیایی پروتئازهای گوارشی در کفشدوزک خربزه مورد بررسی قرار گرفت. اندازه‌گیری فعالیت پروتئازی در لوله گوارش سنین 2، 3 و 4 لاروی و حشرات کامل نر و ماده نشان داد که بیشترین فعالیت آنزیم در لاروهای سن 3 بوده و فعالیت آن در حشرات نر و ماده اختلاف معنیداری با هم ندارند. مقدارpH و دمای بهینه فعالیت پروتئازی در لوله گوارش بهترتیب 5 و 30 درجه سلسیوس بدست آمد. بازدارنده‌های EDTA،TLCK،TPCK،PMSF و یدواستات روی فعالیت پروتئازهای گوارشیE. chrysomelina به ترتیب 93/18، 04/19، 27/22، 05/26 و 32/44 درصد مهارکنندگی نشان دادند. با توجه به بیشترین میزان بازدارندگی ایجاد شده توسط یدواستات روی فعالیت پروتئازی، سیستئین پروتئازها به عنوان پروتئازهای غالب در لوله گوارش این حشره معرفی می‌شوند. نتایج زایموگرام روی ژل نشان داد که آنزیم پروتئاز در لوله گوارشE. chrysomelina ، یک ایزوفرم دارد. | ||
| کلیدواژه‌ها | ||
| کفشدوزک خربزه؛ مهارکننده؛ پروتئاز | ||
| عنوان مقاله [English] | ||
| Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae) | ||
| نویسندگان [English] | ||
| Moloud Gholamzadeh-Chitgar1؛ roohangiz Ghanbarinezhad2؛ Mohammad Ghadamyari3 | ||
| 1Plant Protection Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran | ||
| 2Former student, department of plant protection, faculty of agricultural science, university of guilan | ||
| 3Department of Plant Protection, faculty of agricultural science, university of guilan | ||
| چکیده [English] | ||
| Melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)is a major pest of cucurbitaceous plants by feeding on leaves at larval and adult stages. In this study, biochemical properties of digestive proteases of the melon ladybird were studied in its alimentary canal. Enzyme activity in the digestive systems of 2nd, 3rd and 4th larval instars and adults showed that the highest specific enzyme activities occurred in 3rd instar larvae while no significant differences observed in adults. The optimal pH and temperature for protease activity in alimentary canal were found 5 and 30°C, respectively. The inhibitory effect of EDTA, TLCK, TPCK, PMSF and Iodoacetate were determined 44.32, 26.05, 22.27, 19.04 and 18.93% in the gut of E. chrysomelina, respectively. With respect to the highest inhibition rate in proteolytic activity caused by Iodoacetate, cysteine proteinases are considered as the main proteases in the midgut of the melon ladybird. Zymogram pattern in the native gel indicated that the protease enzyme had one isoform in the alimentary canal of the pest. | ||
| کلیدواژه‌ها [English] | ||
| melon ladybird, inhibitor, protease | ||
| مراجع | ||
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