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اثر تنش خشکی و آسکوربات خارجی بر روی رنگیزه‌های فتوسنتزی، فلاونوئیدها، ترکیبهای فنلی و میزان پراکسیداسیون لیپیدی در گیاه انیسون (Pimpinella anisum L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 3، دوره 25، شماره 4 - شماره پیاپی 46، بهمن 1388، صفحه 456-469 اصل مقاله (271.05 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2010.6999 | ||
| نویسندگان | ||
| ژیکا اسدی کاوان1؛ مه‌لقا قربانلی* 2؛ آرین ساطعی3 | ||
| 1کارشناس ارشد، عضو باشگاه پژوهشگران جوان، گروه زیست‌شناسی، دانشگاه آزاد اسلامی، گرگان | ||
| 2استاد، گروه زیست‌شناسی، دانشگاه آزاد اسلامی، گرگان | ||
| 3استادیار، گروه زیست‌شناسی، دانشگاه آزاد اسلامی، گرگان | ||
| چکیده | ||
| استرس خشکی باعث تحریک ساخته شدن گونه‌های اکسیژن فعال در کلروپلاستهای گیاهی می‌شود و گونه‌های اکسیژن فعال نیز سبب پراکسیداسیون لیپیدهای غشایی و تخریب غشای سلولی می‌شود. انیسون (Pimpinella anisum L.) یکی از گیاهان دارویی بسیار معطر است که ارزش صادراتی فراوانی دارد. در این پژوهش، آسکوربات با هدف اهمیت کنترل استرس اکسیداتیو در تحمل به کمبود آب، بکار گرفته شد و تغییرات محتوای رنگیزه‌ای برگها، ترکیبهای فنلی کل و محتوای مالون دی‌آلدئید بر روی گیاه بررسی شد. طی یک مطالعه گلدانی، تنش خشکی براساس نسبتهای مختلفی از ظرفیت زراعی در سه سطح شاهد، متوسط و شدید (به‌ترتیب 100، 60 و 25 درصد ظرفیت زراعی) و آسکوربات با یک غلظت mM 4/1 به صورت مه‌پاشی اعمال گردید. با پیشرفت تنش، محتوای کلروفیل و نسبت کلروفیل a به کلروفیل b کم شد، در حالی که مقدار فلاونوئید و آنتوسیانین افزایش یافت. همچنین در اثر دهیدراتاسیون، افزایش کاروتن و گزانتوفیل تنها در تنش متوسط گزارش شد. آسکوربات، میزان کلروفیلها و کاروتنوئیدها را زیاد نمود اما میزان فلاونوئید و آنتوسیانین را کاهش داد و تأثیر چشمگیری در افزایش ترکیبهای فنلی کل اندامها در تمام سطوح داشت. همچنین مقدار مالون دی‌آلدئید در شرایط تنش متوسط نسبتاً ثابت ماند ولی در شرایط تنش شدید به‌طور معنی‌دار افزایش یافت که حضور آسکوربات منجر به کاهش مؤثر این متابولیت شد. بنابر نتایج بدست آمده در این پژوهش، آسکوربات خارجی توانست با مکانیسمهای مختلفی توانایی گیاه انیسون را در پاسخ به تنش خشکی افزایش داده و اثر محافظتی در برابر اکسیداسیون لیپیدها ( که ناشی از خشکی می‌باشد) داشته باشد. | ||
| کلیدواژه‌ها | ||
| تنش خشکی؛ تنش اکسیداتیو؛ آسکوربات خارجی؛ فلاونوئیدها؛ پراکسیداسیون لیپیدی؛ انیسون (Pimpinella anisum L.) | ||
| عنوان مقاله [English] | ||
| The effect of drought stress and exogenous ascorbate on photosynthetic pigments, flavonoids, phenol compounds and lipid peroxidation in Pimpinella anisum L. | ||
| نویسندگان [English] | ||
| Zh. Asadi Kavan1؛ M. Ghorbanli2؛ A. Sateei3 | ||
| 1M.Sc. in plant physiology, Member of Young Researchers Club, Islamic Azad University, Gorgan, Iran | ||
| 2Department of Plant Biology, Islamic Azad University, Gorgan, Iran, | ||
| 3Department of Plant Biology, Islamic Azad University, Gorgan, Iran | ||
| چکیده [English] | ||
| Drought stress provokes ROS production in plant cell chloroplasts and subsequently causes lipid membrane peroxidation and damage. Pimpinella anisum L. is one of the aromatic herbal plants which has great export value. The aim of this study was applying exogenous ascorbate in order to control oxidative stress during drought tolerance. Changes of pigment content of leaves, total phenol compounds, malonedialdehyde (MDA) content were measured. In a pot study, drought stress introduced to treatments with 3 replicates based on 3 levels of field capacity (100, 60 and 25%) and ascorbate (1.4 mM) sprayed on them. Chlorophyll content and chlorophyll a/b ratio decreased with increasing in stress levels, while flavonoids and anthocyanins increased. Carotene and xanthophyll increased only in moderate stress level due to drought. Exogenous ascorbate increased chlorophylls and carotenoid content but decreased flavonoid and anthocyanin contents and had great effect on increasing phenol compound in all stress levels. MDA content remained relatively constant, but increased significantly in severe stress levels. Applying exogenous ascorbate led to decreasing metabolite. According to the results exogenous ascorbate could increase the ability of Pimpinella anisum in response to drought stress with different mechanisms and had protective effect against lipid peroxidation due to drought stress. | ||
| کلیدواژه‌ها [English] | ||
| Drought Stress, oxidative stress, exogenous ascorbate, Flavonoids, Lipid peroxidation, Pimpinella anisum L | ||
| مراجع | ||
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