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RAPD ANALYSIS OF GENETIC VARIATION WITHIN AND AMONG NATURAL POPULATIONS OF TWO SPECIES OF DIANTHUS L. (CARYOPHYLLACEAE) IN NE IRAN | ||
| The Iranian Journal of Botany | ||
| مقاله 8، دوره 19، شماره 2، اسفند 2013، صفحه 194-201 اصل مقاله (876.48 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2013.4157 | ||
| نویسندگان | ||
| Maryam Behroozian* 1؛ Azarnoosh Jafari1؛ Mohammad Farsi2 | ||
| 1Departemt of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran | ||
| 2Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| چکیده | ||
| In this study we investigated the genetic variation, using RAPD data in 69 individuals of nine natural populations representing two species of Dianthus L. growing in Northeast of Iran. Eleven RAPD primers generated 111 polymorphic DNA bands. The percentage of polymorphic bands, Nails genetic diversity (h), as well as Shannon index (I), were assessed. A dendrogram based on UPGMA segregated examined populations into two main clusters matching the two species and the two subspecies of Dianthus polylepis subsp. polylepis and D. polylepis subsp. D. binaludensis were to each other. AMOVA showed 82% and 18% of the variation within and among populations respectively. Furthermore, principal coordinate analysis (PCA) based on a Euclidean metric revealed that three populations of D. crinitus subsp. turcomanicus were genetically different from six populations of D. polylepis. According to our results, geographical distances, mating system and gene flow have important effects on genetic polymorphism of populations. | ||
| کلیدواژه‌ها | ||
| Dianthus؛ Caryophyllaceae؛ Iran؛ RAPD markers؛ Genetic variation | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی بین و درون جمعیتهای Caryophyllaceae) Dianthus ) در شمال‌شرق ایران با استفاده از آنالیزRAPD | ||
| نویسندگان [English] | ||
| مریم بهروزیان1؛ آذرنوش جعفری1؛ محمد فارسی2 | ||
| 1دانش آموخته کارشناسی ارشد علوم گیاهی، گروه زیست شناسی ،دانشکده علوم، دانشگاه آزاد اسلامی واحد مشهد. | ||
| 2استاد گروه بیوتکنولوژی گیاهی، پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| چکیده [English] | ||
| در این مطالعه تنوع ژنتیکی 69 فرد از نه جمعیت طبیعی متعلق به دو گونه Dianthus موجود درشمال شرق ایران با استفاده از داده‌های PARD به منظور دستیابی به اطلاعات مهم ژنتیکی بررسی گردید. 111 باند چندشکلی با کمک 11 آغازگر RAPD بدست آمد. درصد باندهای چند شکلی، تنوع ژنتیکی نی و اندیس شانون محاسبه گردید. دندروگرام بر اساس روش UPGMA جمعیتهای مورد بررسی را به دو گروه اصلی مطابق با دو گونه جدا نمود و نزدیکی دو زیرگونهD. polylepis subsp. polylepis وD. polylepis subsp. binaludensis رانسبت به هم نشان داد. بر اساس آنالیز AMOVA به ترتیب 82 و 18 درصد تنوع ژنتیکی برای درون و بین جمعیتها بدست آمد. همچنین آنالیز مختصات اصلی (PCA) بر پایه فاصله اقلیدوسی اختلاف سه جمعیت زیرگونه D. crinitus subsp. turcomanicus را از نظر ژنتیکی با شش جمعیت از دو زیرگونه . polylepis subsp. polylepisDوD. polylepis subsp. binaludensis آشکار کرد. با توجه به نتایج به دست آمده، فاصله جغرافیایی، سیستم آمیزشی و جریان ژنی می تواند اثرات مهمی در تنوع ژنتیکی جمعیتهای جنس Dianthusداشته باشد. | ||
| کلیدواژه‌ها [English] | ||
| Dianthus, Caryophyllaceae, Iran, RAPD markers, Genetic variation | ||
| مراجع | ||
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