| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,087 |
| تعداد مقالات | 34,477 |
| تعداد مشاهده مقاله | 49,207,090 |
| تعداد دریافت فایل اصل مقاله | 79,676,286 |
RAPD ANALYSIS OF GENETIC VARIATION WITHIN AND AMONG NATURAL POPULATIONS OF TWO SPECIES OF DIANTHUS L. (CARYOPHYLLACEAE) IN NE IRAN | ||
| The Iranian Journal of Botany | ||
| مقاله 8، دوره 19، شماره 2، اسفند 2013، صفحه 194-201 اصل مقاله (876.48 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2013.4157 | ||
| نویسندگان | ||
| Maryam Behroozian* 1؛ Azarnoosh Jafari1؛ Mohammad Farsi2 | ||
| 1Departemt of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran | ||
| 2Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| چکیده | ||
| In this study we investigated the genetic variation, using RAPD data in 69 individuals of nine natural populations representing two species of Dianthus L. growing in Northeast of Iran. Eleven RAPD primers generated 111 polymorphic DNA bands. The percentage of polymorphic bands, Nails genetic diversity (h), as well as Shannon index (I), were assessed. A dendrogram based on UPGMA segregated examined populations into two main clusters matching the two species and the two subspecies of Dianthus polylepis subsp. polylepis and D. polylepis subsp. D. binaludensis were to each other. AMOVA showed 82% and 18% of the variation within and among populations respectively. Furthermore, principal coordinate analysis (PCA) based on a Euclidean metric revealed that three populations of D. crinitus subsp. turcomanicus were genetically different from six populations of D. polylepis. According to our results, geographical distances, mating system and gene flow have important effects on genetic polymorphism of populations. | ||
| کلیدواژهها | ||
| Dianthus؛ Caryophyllaceae؛ Iran؛ RAPD markers؛ Genetic variation | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی بین و درون جمعیتهای Caryophyllaceae) Dianthus ) در شمالشرق ایران با استفاده از آنالیزRAPD | ||
| نویسندگان [English] | ||
| مریم بهروزیان1؛ آذرنوش جعفری1؛ محمد فارسی2 | ||
| 1دانش آموخته کارشناسی ارشد علوم گیاهی، گروه زیست شناسی ،دانشکده علوم، دانشگاه آزاد اسلامی واحد مشهد. | ||
| 2استاد گروه بیوتکنولوژی گیاهی، پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| چکیده [English] | ||
| در این مطالعه تنوع ژنتیکی 69 فرد از نه جمعیت طبیعی متعلق به دو گونه Dianthus موجود درشمال شرق ایران با استفاده از دادههای PARD به منظور دستیابی به اطلاعات مهم ژنتیکی بررسی گردید. 111 باند چندشکلی با کمک 11 آغازگر RAPD بدست آمد. درصد باندهای چند شکلی، تنوع ژنتیکی نی و اندیس شانون محاسبه گردید. دندروگرام بر اساس روش UPGMA جمعیتهای مورد بررسی را به دو گروه اصلی مطابق با دو گونه جدا نمود و نزدیکی دو زیرگونهD. polylepis subsp. polylepis وD. polylepis subsp. binaludensis رانسبت به هم نشان داد. بر اساس آنالیز AMOVA به ترتیب 82 و 18 درصد تنوع ژنتیکی برای درون و بین جمعیتها بدست آمد. همچنین آنالیز مختصات اصلی (PCA) بر پایه فاصله اقلیدوسی اختلاف سه جمعیت زیرگونه D. crinitus subsp. turcomanicus را از نظر ژنتیکی با شش جمعیت از دو زیرگونه . polylepis subsp. polylepisDوD. polylepis subsp. binaludensis آشکار کرد. با توجه به نتایج به دست آمده، فاصله جغرافیایی، سیستم آمیزشی و جریان ژنی می تواند اثرات مهمی در تنوع ژنتیکی جمعیتهای جنس Dianthusداشته باشد. | ||
| کلیدواژهها [English] | ||
| Dianthus, Caryophyllaceae, Iran, RAPD markers, Genetic variation | ||
| مراجع | ||
|
Bharmauria, V., Narang, N., Verma, V. & Sharma, Sh. 2009: Genetic variation and polymorphism in the Himalayan nettle plant Urtica dioica based on RAPD marker. –J Med Plants Res. 3(3): 166-170. Bown, D. 1995: The Royal Horticultural Society Encyclopaedia of Herbs and their Uses. -Dorling Kindersley, London. Carine, C. L. & J. A. Shykoff. 2003: Outcrossing rates in the gynomonoecious – gynodioecious species Dianthus sylvestris. –Am. J. Bot.90: 579-585. Chase, M. R., D. H. Boshier, & K. S. Bawa. 1995: Population genetics of Cordia alliodora (Boraginaceae), a neotropical tree. 1. Genetic variation in natural populations. – Am. J. Bot. 82: 468-475. Crespi, A., C. F. Fernandis, A. Castro, S. Berrnardos, & F. Amich. 2007: Morpho-enviromental characterization of the genus Dianthus (Caryophylaceae) in the Iberian Peninsula: D. pungens group. - Ann. Bot. fennici 44: 241-255. Dellaporta, S. L., Wood, & J. B. Hicks. 1983: A plant DNA minipreparation: version II. -Plant Mol. Biol. Rep. 1 (14):19-21. Excoffier, L., P. E. Smouse, & J. M. Q. Uattor 1992: Analysis of molecular variance inferred from metric distance among DNA haplotypes: Application to human mitochondrial DNA restriction data. -Genet. 131: 479–491. Farsi, M., M. Behroozian, J. Vaezi, M. R. Joharchi, & F. Memariani. 2013: The evolution of Dianthus polylepis complex (Caryophyllaceae) inferred from morphological and nuclear DNA sequence data: one or two species? –Plant Syst. Evol. 299: 1419-1431. Galbally, J. & E. Galbally. 1997: Carnations and pinks for garden and greenhouses. -Timber Press, Portland, Oregon, USA. pp 1-310. Ge, X. J. 1998: Retrospective and prospective of population genetic structure in plants. Li C-S. -Adv. Plant. Sci. 1. 1-16. Gower, J. C. 1966: Some distance properties of latent root and vector methods used in multivariate analysis. -Biometrika 53: 325–338. Holley, W. D., & R. Baker 1963: Carnation Production: Including the History, Breeding, Culture and Marketing of Carnations. -W.C. Brown Co Ltd., Dubuque, Iowa, USA. Ingwerson, W. 1949: The Dianthus. -Collins, pp 1-128. Holsinger, K. T., P. O. Lewis, & D. K. Dey. 2002: A Bayesian approach to inferring population structure from dominant markers. -Mol. Ecol. 11: 1157–1164. Jafari, A. & M. Behroozian. 2010: A cytotaxonomic study on Dianthus species in northeast of Iran. – Asian J. Plant Sci. 9 (1): 58–62. Jurgens, A., Witt, T. & Gottsberger, G. 2003: Flower scent composition in Dianthus and Saponaria species. -Biochemical Systematics and Ecology 31: 345-357. Kimura, T., M. Yagi, C. Nishitani, T. Onozaki, Y. Ban, & T. Yamamoto. 2009: Development of SSR Markers in Carnation (Dianthus caryophyllus). -J. Japan. Soc. Hort. Sci. 78 (1): 115–123. Lee, S. Y., B. W. Yae, & K. S. Kim 2005: Segregation patterns of several morphological characters and RAPD markers in interspecific hybrids between Dianthus giganteus and D. carthusianorum. –Sci. Hort. 105: 53-64. Lewontin, R. C. 1972: The apportionment of human diversity. -Evol. Bio. 6:381–398. Lin, JJ., J. Kuo, J. Ma, J. A. Saunders, H. S. Beard, M. H. Macdonald, W. Kenworthy, G. N. Ude & B. F. Mathews. 1996: Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques. -Plant Mol. Biol. Rep. 14: 156-69. Loveless, M. D. 1992: Isozyme variation in tropical trees: patterns of genetic organization. -New Forests 6: 67-94. McGeorge, P. & K. Hammett. 2002: Carnations and Pinks. -David Bateman Ltd, Auckland. Pp. 1-96. Nimura, M., J. Kato, & M. Mii. 2006: Interspecific hybrid production by reciprocal crosses between Dianthus caryophyllus L. and Dianthus × isensis Hirahata et Kitamura. -J. Hort. Sci. Biotechnol, 81: 995–1001. Onozaki, T., T. Yoshinari, T. Yashimura, M. Yagi, S. Yoshioka, M. Taneya, & M. Shibata 2006: DNA Markers Linked to a Recessive Gene Controlling Single Flower Type Derived from a Wild Species, Dianthus capitatus ssp. andrzejowskianus. -Horticultural Research ( Japan ) 5: 363-367. Peakall, R. & P. E. Smouse 2001: GENALEx V5.1: Genetic analysis in Excel. Population genetic software for teaching and research. -Australian National University. Canberra. Australia. http://www.anu.edu.au/BoZo/GenAlEx/. Rechinger, K. H. 1986: Dianthus (Caryophyllaceae) in K. H. Rechinger Flora Iranica 163:128-188. -Graz. Safari, S., A. A. Mehrabi, & Z. Safari 2013: Efficiency of RAPD and ISSR markers in assessment of genetic diversity in Brassica napus genotypes. -IJACS. In press. Sheng, H. M., L. Z. An, S. J. Xu, G. X. Liu, X. L. Zheng, L. L. Pu, Y. J. Lin, & Y. S. Lian 2006: Analysis of the genetic diversity and relationships among and within species of Hippophae (Elaeagnaceae) based on RAPD markers. -Plant Syst. Evol. 260: 25-37 Su, Yeons, K. 2002: Genetic Relationship among Korean Dianthus Species Based on Morphological Characteristics and RAPD Analysis. http://www.niha.go.kr/report_board/report_board_view.asp? size_tag=&sno=1726&gubun=&p_type= 11 Van Altvorst, V. A. C. & A. G. Bovy 1995: The role of ethylene in senescence of carnation flowers. -Plant Growth Reg. 16: 43–53. Wei, X., H. L. Cao, Y. SH. Jiang, W. H. Ye, X. J. Ge, & F. Li. 2008: Population genetic structure of Camellia nitidissima (Theaceae) and conservation implications. -Botanical Studies 49: 147-153. Woltering, E. J., A. Ten Have, P. B. Larsen, & W. R. Woodson 1994: Ethylene biosynthetic genes and inter-organ signaling during flower senescence. In: Scott RJ, Stead AD (eds.) Molecular and cellular aspects of plant reproduction. -Cambridge University Press, Cambridge, UK, 55: 285–307. Zarek, M. 2009: RAPD analysis of genetic structure four natural populations of Taxus baccata from southern Poland. -Acta Biol. Cracov. Bot. 51/2: 67–75.
| ||
|
آمار تعداد مشاهده مقاله: 2,434 تعداد دریافت فایل اصل مقاله: 2,109 |
||