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جهاندیده مهجن آبادی, وحید اله, اسدی رحمانی, هادی, شمشیری پور, مهدیه, خاوازی, کاظم. (1404). بهبود رشد و جذب عناصر غذایی ذرت با محلول‌پاشی باکتری‌های محرک رشد فیلوسفر در شرایط مزرعه. سامانه مدیریت نشریات علمی, 13(2), 213-228. doi: 10.22092/sbj.2026.370545.285
وحید اله جهاندیده مهجن آبادی; هادی اسدی رحمانی; مهدیه شمشیری پور; کاظم خاوازی. "بهبود رشد و جذب عناصر غذایی ذرت با محلول‌پاشی باکتری‌های محرک رشد فیلوسفر در شرایط مزرعه". سامانه مدیریت نشریات علمی, 13, 2, 1404, 213-228. doi: 10.22092/sbj.2026.370545.285
جهاندیده مهجن آبادی, وحید اله, اسدی رحمانی, هادی, شمشیری پور, مهدیه, خاوازی, کاظم. (1404). 'بهبود رشد و جذب عناصر غذایی ذرت با محلول‌پاشی باکتری‌های محرک رشد فیلوسفر در شرایط مزرعه', سامانه مدیریت نشریات علمی, 13(2), pp. 213-228. doi: 10.22092/sbj.2026.370545.285
جهاندیده مهجن آبادی, وحید اله, اسدی رحمانی, هادی, شمشیری پور, مهدیه, خاوازی, کاظم. بهبود رشد و جذب عناصر غذایی ذرت با محلول‌پاشی باکتری‌های محرک رشد فیلوسفر در شرایط مزرعه. سامانه مدیریت نشریات علمی, 1404; 13(2): 213-228. doi: 10.22092/sbj.2026.370545.285

بهبود رشد و جذب عناصر غذایی ذرت با محلول‌پاشی باکتری‌های محرک رشد فیلوسفر در شرایط مزرعه

زیست شناسی خاک
مقاله 6، دوره 13، شماره 2، بهمن 1404، صفحه 213-228    اصل مقاله (1.36 M)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22092/sbj.2026.370545.285
نویسندگان
وحید اله جهاندیده مهجن آبادی* ؛ هادی اسدی رحمانی؛ مهدیه شمشیری پور؛ کاظم خاوازی
موسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.
چکیده
فیلوسفر گیاهان، زیستگاه مجموعه‌ای متنوع از ریزجانداران مفید از جمله باکتری‌های محرک رشد گیاه (PGPB) است که پتانسیل قابل‌توجهی برای بهبود رشد و عملکرد گیاهان دارند. این پژوهش با هدف بررسی تأثیر محلول‌پاشی برگی باکتری‌های بومی جداسازی‌شده از فیلوسفر ذرت بر عملکرد و جذب عناصر غذایی این گیاه در شرایط مزرعه‌ای انجام شد. آزمایش به صورت طرح بلوک‌های کامل تصادفی با سه تکرار در سال زراعی ۱۴۰۰-۱۳۹۹ اجرا گردید. تیمارهای آزمایشی شامل محلول‌پاشی برگی با سه گونه باکتری Enterobacter hormaechei (AC. MN099393)،  Stenotrophomonas maltophilia (AC. MN099392) و Microbacterium arborescens (AC. MN099379) و همچنین یک تیمار شاهد (بدون باکتری) بود. نتایج نشان داد که کاربرد باکتری‌های E. hormaechei و S. maltophilia به‌طور معنی‌داری وزن خشک اندام هوایی ذرت را به­ترتیب 10/7 و 11 درصد در مقایسه با شاهد افزایش دادند. بیشترین افزایش در غلظت نیتروژن (26/66درصد) مربوط به تیمار E. hormaechei بود. همچنین، این دو باکتری منجر به افزایش معنی‌دار غلظت آهن اندام هوایی (به ترتیب 37/3درصد و 33/2درصد) شدند. همچنین باکتری E. hormaechei منجر به افزایش معنی‌دار غلظت منگنز اندام هوایی (39درصد) شد.  با این حال، تأثیر تیمارها بر وزن تر اندام هوایی و غلظت فسفر، پتاسیم، روی و مس معنی‌دار نبود. یافته‌ها مؤید این است که محلول‌پاشی برگی با باکتری‌های محرک رشد فیلوسفر، به‌ویژه سویه‌های E. hormaechei و S. maltophilia، می‌تواند به‌عنوان یک راهکار زیست‌محیطی و مؤثر در جهت بهبود عملکرد و ارتقای وضعیت تغذیه‌ای گیاه ذرت در سیستم‌های کشاورزی پایدار مورد بهره‌برداری قرار گیرد.
کلیدواژه‌ها
باکتری‌های محرک رشد گیاه؛ فیلوسفر؛ کاربرد برگی؛ عملکرد ذرت؛ جذب عناصر غذایی
عنوان مقاله [English]
Enhancing maize growth and nutrient uptake through foliar application of phyllosphere-derived plant growth-promoting bacteria under field conditions
نویسندگان [English]
Vahid Alah Jahandideh Mahjen Abadi؛ Hadi Asadi Rahmani؛ Mahdieh Shamshiripour؛ Kazem Khavazi
Soil and Water Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
چکیده [English]
Background and Objectives: The phyllosphere microbiome represents an extraordinary ecological niche that harbors diverse microbial communities with immense potential for agricultural applications. As a unique aerial habitat, the phyllosphere supports bacterial populations that have evolved sophisticated mechanisms for plant growth promotion and stress mitigation. While rhizosphere bacteria have been extensively exploited in agricultural practices, the systematic utilization of phyllosphere-derived plant growth-promoting bacteria (PGPB) as foliar bioinoculants remains comparatively under-investigated, particularly under real-field conditions. This knowledge gap assumes critical importance given the increasing global demands for sustainable agricultural intensification and food security. The current investigation was designed to address this research vacuum through a comprehensive evaluation of three meticulously selected phyllosphere-originating bacterial strains Enterobacter hormaechei (AC. MN099393), Stenotrophomonas maltophilia (AC. MN099392), and Microbacterium arborescens (AC. MN099379) for their efficacy in enhancing maize (Zea mays L.) productivity, nutrient acquisition efficiency, and overall nutritional quality through foliar application. The study specifically aimed to elucidate the effects of these bacterial inoculants on shoot dry and fresh weight, nutrient uptake, while establishing correlations between observed phenotypic improvements and the documented plant growth-promoting attributes of the selected bacterial strains.




Materials and Methods: A rigorously designed field experiment was implemented during the 2020-2021 growing season at the Soil and Water Research Institute in Karaj, Iran, utilizing a randomized complete block design with three replications to ensure statistical robustness. The experimental matrix comprised four distinct treatments: (1) Absolute control (non-inoculated); (2) E. hormaechei inoculation; (3) S. maltophilia inoculation; and (4) M. arborescens inoculation. Soil characteristics were thoroughly analyzed before experiment establishment, revealing a loam texture with specific chemical properties. For the cultivation of maize, the seed variety 704 was used. The area of ​​each plot was 30 m², comprising 4 ridges with a distance of 75 centimeters and a length of 10 m. Bacterial suspensions were prepared to achieve optimal concentration (109 CFU mL⁻¹) and applied at the critical V3 phenological stage (4-6 leaf stage) during evening hours to maximize phyllosphere colonization efficiency and minimize UV-induced bacterial mortality. The application method involved careful dilution protocols and uniform spraying using sterilized equipment to ensure consistent coverage. Comprehensive assessments included detailed analysis of shoot dry and fresh weight and precise quantification of macronutrient (N, P, K) and micronutrient (Fe, Zn, Cu, Mn) concentrations through standard protocols. Statistical analyses of the data were performed using the SAS software. Mean comparisons were conducted using Fisher's Least Significant Difference (LSD) test at the 5% probability level.
Results: The foliar application of phyllosphere bacteria significantly influenced maize growth parameters. Shoot dry weight was markedly increased by both bacterial treatments (S. maltophilia and E. hormaechei ) compared to the non-inoculated control (14289 kg ha⁻¹). Application of S. maltophilia resulted in a shoot dry weight of 15859 kg ha⁻¹, representing a significant increase of 11.0%. Similarly, E. hormaechei treatment yielded a dry weight of 15813 kg ha⁻¹, an increase of 10.7%. Nutritional analysis revealed significant biofortification effects, particularly for E. hormaechei. This treatment increased nitrogen concentration by 26.66% (1.33% versus control 1.05%), iron content by 37.3% (53.4 mg kg⁻¹ versus 38.9 mg kg⁻¹), and manganese concentration by 39% (35.3 mg kg⁻¹ versus 25.4 mg kg⁻¹). The S. maltophilia treatment also significantly enhanced nitrogen and iron concentrations by 19.02% and 33.16%, respectively. Phosphorus, potassium, zinc, and copper levels showed non-significant responses across treatments. M. arborescens application showed limited efficacy across most measured parameters, potentially attributable to suboptimal environmental adaptation mechanisms or reduced phyllosphere colonization capacity.




Conclusion: This research provides compelling evidence that targeted foliar application of specific phyllosphere-derived PGPB strains, particularly E. hormaechei and S. maltophilia, constitutes an innovative and highly effective strategy for enhancing maize productivity and nutritional quality under field conditions. The demonstrated improvements are mechanistically linked to the bacteria's multifunctional plant growth-promoting attributes, including enhanced nitrogen fixation capacity, sophisticated phytohormone modulation, and efficient siderophore-mediated iron sequestration. The significant enhancement of nutrient concentrations represents a major advancement toward sustainable nutrient management practices. These findings establish a robust scientific foundation for developing next-generation foliar bioinoculant technologies that can significantly reduce dependence on chemical fertilizers while simultaneously addressing global food security and nutritional challenges. The study opens new avenues for sustainable agricultural innovation through strategic manipulation of phyllosphere microbiomes and provides crucial insights for optimizing application protocols, strain selection criteria, and integration with existing agricultural practices. Future research should focus on elucidating molecular mechanisms underlying plant-bacteria interactions in the phyllosphere and developing commercial formulations for large-scale agricultural implementation.
کلیدواژه‌ها [English]
Plant growth-promoting bacteria, Phyllosphere, Foliar application, Zea mays, Nutrient uptake
مراجع
  1. Ahmed, E., & Holmström, S. J. (2014). Siderophores in environmental research: roles and applications. Microbial Biotechnology, 7(3), 196-208. https://doi.org/1111/1751-7915.12117
  2. AOAC (Association of Official Analytical Chemists). (2005). Official methods of analysis, 18th edn. AOAC-Int, Arlington.
  3. Aslantaş, R., Cakmakçi, R., & Şahin, F., (2007). Effect of plant growth promoting rhizobacteria on young apple tree growth and fruit yield under orchard conditions. Scientia Horticulturae, 111(4), 371-377. https://doi.org/10.1016/j.scienta.2006.12.016
  4. Batool, F., Rehman, Y., & Hasnain. S., (2016). Phylloplane associated plant bacteria of commercially superior wheat varieties exhibit superior plant growth promoting abilities. Frontiers in Life Science, 9(4), 313-322. https://doi.org/10.1080/21553769.2016.1256842
  5. Bokulich, N. A., Thorngate, J. H., Richardson, P. M., & Mills, D. A. (2014). Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate. Proceedings of the National Academy of Sciences, 111(1), E139-E148. https://doi.org/1073/pnas.1317377110
  6. Cardozo, P., Di Palma, A., Martin, S., Cerliani, C., Esposito, G., Reinoso, H., & Travaglia, C. (2022). Improvement of maize yield by foliar application of Azospirillum brasilenseJournal of Plant Growth Regulation, 41(3), 1032-1040.‏ https://doi.org/10.1007/s00344-021-10356-9
  7. Carrillo, A. E., Li, C. Y., & Bashan, Y. (2002). Increased acidification in the rhizosphere of cactus seedlings induced by Azospirillum brasilense. Naturwissenschaften, 89(9), 428-432. https://doi.org/10.1007/s00114-002-0347-6
  8. Chaudhary, D., Kumar, R., Sihag, K., & Kumari, A. (2017). Phyllospheric microflora and its impact on plant growth: A review. Agricultural Reviews, 38 (1). https://doi.org/10.18805/ag.v0iOF.7308
  9. Devarajan, A. K., Muthukrishanan, G., Truu, J., Truu, M., Ostonen, I., Kizhaeral S, S., ... & Kuttalingam Gopalasubramanian, S. (2021). The foliar application of rice phyllosphere bacteria induces drought-stress tolerance in Oryza sativa (L.). Plants, 10(2), 387.‏ https://doi.org/10.3390/plants10020387
  10. Dourado, M. N., Aparecida Camargo Neves, A., Santos, D. S., & Araújo, W. L. (2015). Biotechnological and Agronomic Potential of Endophytic Pink‐Pigmented Methylotrophic MethylobacteriumBioMed Research International, 2015(1), 909016.‏ https://doi.org/10.1155/2015/909016
  11. Esitken, A., Yildiz, H. E., Ercisli, S., Donmez, M. F., Turan, M., & Gunes, A. (2010). Effects of plant growth promoting bacteria (PGPB) on yield, growth and nutrient contents of organically grown strawberry. Scientia Horticulturae, 124(1), 62-66.‏ https://doi.org/10.1016/j.scienta.2009.12.012
  12. Fallah Nosratabad, A., & Khoshru, B. (2024). Potentials and challenges of biofertilizers in sustainable agriculture. Soil Science Society of Iran, 12(1), 19-63. https://doi.org/10.22092/SBJ.2024.36 6090.265
  13. Ferreira, C. M., Vilas-Boas, Â., Sousa, C. A., Soares, H. M., & Soares, E. V. (2019). Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions. AMB Express, 9(1), 78.  https://doi.org/10.1186/s13568-019-0796-3
  14. Finkel, O. M., Castrillo, G., Paredes, S. H., González, I. S., & Dangl, J. L. (2017). Understanding and exploiting plant beneficial microbes. Current Opinion in Plant Biology, 38, 155-163. https://doi.org/10.1016/j.pbi.2017.04.018
  15. Freitas, G. D. S., Moreira, A., Prudencio, M. F., Heinrichs, R., Nogueira, M. A., Hungria, M., & Soares Filho, C. V. (2023). Foliar spray inoculation with plant growth promoting bacteria associated with nitrogen doses in Megathyrsus maximus BRS Zuri. Agronomy, 13(4), 1040.‏ https://doi.org/10.3390/agronomy13041040
  16. Gao, J., Luo, Y., Wei, Y., Huang, Y., Zhang, H., He, W., Sheng, H., & An, L. (2019). Screening of plant growth promoting bacteria (PGPB) from rhizosphere and bulk soil of Caragana microphylla in different habitats and their effects on the growth of Arabidopsis seedlings. Biotechnology & Biotechnological Equipment, 33(1), 921-930. https://doi.org/1080/13102818.2019.1629841
  17. Gee, G.W., & Bauder, J.W. 1986. Particle size analysis. P 383-411, In: A. Klute (Ed.),
    Methods of soil analysis, Part I. 2d Madison, WI. https://doi.org/10.2136/sssabookser5.1.2ed.c15
  18. Gheibi, M.N., Tehrani, M.M., & Asadi, F. (2014). A Guide to the Integrated Management of Soil Fertility and Corn Nutrition. Soil and Water Research Institute Publications. 53 pp. (In Perisan)
  19. Giri, S., & Pati, B., (2004). A comparative study on phyllosphere nitrogen fixation by newly isolated Corynebacterium & Flavobacterium sp. and their potentialities as biofertilizer. Acta Microbiologica et Immunologica Hungarica, 51(1-2), 47-56. https://doi.org/10.1556/AMicr.51.2004.1-2.3
  20. Hirano, S. S., & Upper, C. D. (2000). Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae—a pathogen, ice nucleus, and epiphyte. Mol. Biol. Rev. 64 (3):624-653. https://doi.org/10.1128/MMBR.64.3.624-653.2000
  21. Jackson, C., Stone, B., & Tyler, H. (2015). Emerging perspectives on the natural microbiome of fresh produce vegetables. Agriculture, 5(2), 170-187. https://doi.org/10.3390/agriculture5020170
  22. Jahandideh Mahjen Abadi, V. A., Sepehri, M., Rahmani, H. A., Zarei, M., Ronaghi, A., Taghavi, S. M., & Shamshiripour, M. (2020). Role of dominant phyllosphere bacteria with plant growth–promoting characteristics on growth and nutrition of maize (Zea mays). Journal of Soil Science and Plant Nutrition, 20, 2348-2363. https://doi.org/10.1007/s42729-020-00302-1
  23. Javadzadeh, M., Khavazi, K., Ghanavati, N., Jafarnejadi, A. R., Jahandideh Mahjenabadi, V. A. (2024). Effect of plant growth-promoting bacteria and sulfur on growth and micronutrient concentration in wheat grain in saline-sodic soils. Soil Science Society of Iran, 11(2), 167-182. https://doi.org/10.22092/sbj.2024.356053.225
  24. Khoshru, B.,  Fallah Nosratabad, A., Khosravi, H., Asgharzadeh, A., Faridian, L. (2025). nhancing agricultural productivity using PGPR and nanoparticles: mechanisms, challenges, and future directions. Soil Science Society of Iran, 12(2), 279-313. https://doi.org/ 10.22092/SBJ.2025.368425. 277
  25. Knief, C., Delmotte, N., Chaffron, S., Stark, M., Innerebner, G., Wassmann, R., Von Mering, C., & Vorholt, J. A. (2012). Metaproteogenomic analysis of microbial communities in the phyllosphere and rhizosphere of rice. The Isme Journal, 6(7), 1378. https://doi.org/10.1038/ismej.2011.192
  26. Kudoyarova, G. R., Vysotskaya, L. B., Arkhipova, T. N., Kuzmina, L. Y., Galimsyanova, N. F., Sidorova, L. V., Gabbasova, I. M., Melentiev, A. I., & Veselov, S. Y. (2017). Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiologiae Plantarum, 39(11), 253. https://doi.org/10.1007/s11738-017-2556-9
  27. Lindow, S. E., & Brandl, M. T. (2003). Microbiology of the phyllosphere. Applied and Environmental Microbiology, 69(4), 1875-1883.‏https://doi.org/1128/AEM.69.4.1875-1883.2003
  28. Madhaiyan, M., Poonguzhali, S., Senthilkumar, M., Seshadri, S., Chung, H., Jinchul, Y., Sundaram, S., & Tongmin, S., (2004). Growth promotion and induction of systemic resistance in rice cultivar Co-47 (Oryza sativa) by Methylobacterium spp. Botanical Bulletin of Academia Sinica, 45.
  29. Mwajita, M. R., Murage, H., Tani, A., & Kahangi, E. M. (2013). Evaluation of rhizosphere, rhizoplane and phyllosphere bacteria and fungi isolated from rice in Kenya for plant growth promoters. SpringerPlus, 2(1), 606. https://doi.org/10.1186/2193-1801-2-606
  30. Page, A. L., Miller, R. H., & Keeny, D. R. (1982). Methods of soil analysis. Part 2, Chemical and Microbiological Properties, Madison Wisconsin U.S.A.
  31. Parasuraman, P., Pattnaik, S., & Busi, S. (2019). Phyllosphere Microbiome: Functional Importance in Sustainable Agriculture. In New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier, 135-14 https://doi.org/10.1016/B978-0-444-64191-5.00010-9
  32. Pirlak, L., Turan, M., Sahin, F., & Esitken, A., (2007). Floral and foliar application of plant growth promoting rhizobacteria (PGPR) to apples increases yield, growth, and nutrient element contents of leaves. Journal of Sustainable Agriculture, 30(4), 145-155. https://doi.org/10.1300/J064v30n04_11
  33. Ranawat, B., Bachani, P., Singh, A., & Mishra, S. (2021). Enterobacter hormaechei as plant growth-promoting bacteria for improvement in Lycopersicum esculentum. Current microbiology, 78(4), 1208-1217.‏ https://doi.org/10.1007/s00284-021-02368-1
  34. Ryu, J., Madhaiyan, M., Poonguzhali, S., Yim, W., Indiragandhi, P., Kim, K., Anandham, R., Yun, J., Kim, K.H., & Sa, T. (2006). Plant growth substances produced by Methylobacterium spp. and their effect on tomato (Lycopersicon esculentum) and red pepper (Capsicum annuum L.) growth. Journal of Microbiology and Biotechnology, 16(10), 1622.
  35. Senthilkumar, M., Madhaiyan, M., Sundaram, S., & Kannaiyan, S. (2009). Intercellular colonization and growth promoting effects of Methylobacterium with plant-growth regulators on rice (Oryza sativa L. Cv CO-43). Microbiological Research, 164(1), 92-104. https://doi.org/10.1016/j.micres.2006.10.007
  36. Shamshiripour, M., Jahandideh Mahjen Abadi, V.A., Hemmati, V., Otadi, A., & Esmaeilizad, A. (2023). Determining the appropriate formulation for growth promotong bacterial inoculum of Corn phyllosphere. Soil and Water Research Institute Publications. 49 pp. (In Perisan)
  37. Sharma, P., Pandey, R., & Chauhan, N. S. (2024). Biofertilizer and biocontrol properties of Stenotrophomonas maltophilia BCM emphasize its potential application for sustainable agriculture. Frontiers in Plant Science, 15, 1364807.‏ https://doi.org/10.3389/fpls.2021.680981
  38. Sharma, S., Chandra, S., Kumar, A., Bindraban, P., Saxena, A. K., Pande, V., & Pandey, R. (2019). Foliar application of iron fortified bacteriosiderophore improves growth and grain Fe concentration in wheat and soybean. Indian Journal of Microbiology, 59(3), 344-350. https://doi.org/10.1007/s12088-019-00810-4
  39. Shiferaw, B., Prasanna, B. M., Hellin, J., & Bänziger, M. (2011). Crops that feed the world 6. Past successes and future challenges to the role played by maize in global food security. Food Security, 3(3), 307. https://doi.org/10.1007/s12571-011-0140-5
  40. Tani, A., Sahin, N., Fujitani, Y., Kato, A., Sato, K., & Kimbara, K., (2015). Methylobacterium species promoting rice and barley growth and interaction specificity revealed with whole-cell matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) analysis. PloS one, 10(6), e01295 https://doi.org/10.1371/journal.pone.0129509
  41. Thomas, G.W. 1996. Soil pH and Soil Acidity. P 475-490, In: D.L. Sparks (Ed.), Methods of soil analysis, Part 3, No. 5, ASA and SSSA, Madison, WI. https://doi.org/10.2136/sssabookser5.3.c16
  42. Vorholt, J. A. (2012). Microbial life in the phyllosphere. Nature Reviews Microbiology, 10(12), 828. https://doi.org/10.1038/nrmicro2910
  43. Walkley, A., & Black, I.A. (1934). An examination of degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 37, 29-38. https://doi.org/1097/00010694-193401000-00003
  44. Yen, K. S., Sundar, L. S., & Chao, Y. Y. (2022). Foliar application of Rhodopseudomonas palustris enhances the rice crop growth and yield under field conditions. Plants, 11(19), 2452.‏ https://doi.org/10.3390/plants11192452.
آمار
تعداد مشاهده مقاله: 25
تعداد دریافت فایل اصل مقاله: 17


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