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تفرجی, سید حسن. (1404). توزیع فراوانی و شناسایی مولکولی باکتری‌های بومی محرک رشد در جنوب استان فارس. سامانه مدیریت نشریات علمی, 13(2), 193-211. doi: 10.22092/sbj.2025.371124.289
سید حسن تفرجی. "توزیع فراوانی و شناسایی مولکولی باکتری‌های بومی محرک رشد در جنوب استان فارس". سامانه مدیریت نشریات علمی, 13, 2, 1404, 193-211. doi: 10.22092/sbj.2025.371124.289
تفرجی, سید حسن. (1404). 'توزیع فراوانی و شناسایی مولکولی باکتری‌های بومی محرک رشد در جنوب استان فارس', سامانه مدیریت نشریات علمی, 13(2), pp. 193-211. doi: 10.22092/sbj.2025.371124.289
تفرجی, سید حسن. توزیع فراوانی و شناسایی مولکولی باکتری‌های بومی محرک رشد در جنوب استان فارس. سامانه مدیریت نشریات علمی, 1404; 13(2): 193-211. doi: 10.22092/sbj.2025.371124.289

توزیع فراوانی و شناسایی مولکولی باکتری‌های بومی محرک رشد در جنوب استان فارس

زیست شناسی خاک
مقاله 5، دوره 13، شماره 2، بهمن 1404، صفحه 193-211    اصل مقاله (1.54 M)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22092/sbj.2025.371124.289
نویسنده
سید حسن تفرجی*
گروه کشاورزی، دانشگاه پیام نور، تهران، ایران
چکیده
با توجه به چالش‌های کشاورزی پایدار و نیاز به کاهش مصرف نهاده‌های شیمیایی، استفاده از روشهای سازگار با محیط‌زیست و شناسایی باکتری‌های محرک رشد بومی از اهمیت ویژه‌ای برخوردار است. این پژوهش با هدف بررسی نظام‌مند توزیع فراوانی و شناسایی باکتری‌های محرک رشد در ریزوسفر گیاهان مختلف جنوب استان فارس انجام شد. در این مطالعه، 181 جدایه باکتریایی از ریزوسفر 45 نمونه گیاهی مختلف جداسازی و از نظر صفات محرک رشد اولیه شامل تولید سیدروفور، آمونیاک، انحلال فسفات، آزادسازی پتاسیم و انحلال روی ارزیابی شدند. نتایج نشان داد که 94 جدایه (52 درصد) دارای حداقل یک صفت محرک رشد بودند و توزیع فراوانی صفات، حاکی از شیوع بیشتر مکانیسم‌های تولید سیدروفور و آمونیاک در بین جامعه باکتریایی بود. در مرحله بعد، 12 جدایه برتر انتخاب و از نظر توانایی تولید اکسین و فعالیت آنزیم ACC دآمیناز مورد ارزیابی کمی قرار گرفتند. همه جدایه‌های منتخب قادر به تولید IAA بودند که بیشترین میزان مربوط به جدایه‌های SF1050 و SF1078 به ترتیب با 52/10 و 51/30 میکروگرم بر میلی‌لیتر بود. از این میان، 7 جدایه فاقد فعالیت آنزیم ACC دآمیناز بودند و جدایه SF1044 با تولید 306/93 نانومول آلفا-کتوبوتیرات بر میلی‌گرم پروتئین در ساعت، بالاترین فعالیت را نشان داد. شناسایی مولکولی جدایه‌های برتر، تعلق آن‌ها را به جنس‌های Bacillus، Pseudomonas، Acinetobacter، Pseudarthrobacter و Lysinibacillus تأیید کرد. مهم‌ترین دستاورد نوآورانه این پژوهش، شناسایی جدایه SF1044 به عنوان Pseudomonas sp. بود که به طور همزمان واجد تمام هفت صفت محرک رشد مورد آزمایش بود. این پژوهش گامی مؤثر در جهت شناسایی و بهره‌برداری از ظرفیت‌های میکروبی بومی برای توسعه کودهای زیستی ترکیبی و پایدار در منطقه جنوب استان فارس محسوب می‌شود.
کلیدواژه‌ها
اکسین؛ باکتری‌های محرک رشد؛ توزیع فراوانی؛ ریزوسفر؛ شناسایی مولکولی؛ ACC دآمیناز
عنوان مقاله [English]
Frequency Distribution and Molecular Identification of Indigenous Plant Growth-Promoting Rhizobacteria in Southern Fars Province
نویسندگان [English]
Seyed Hassan Tafaroji
Department of Agriculture, Payame Noor University, Tehran, Iran
چکیده [English]
Background and Objectives: The increasing global population and challenges related to food security have underscored the critical need for sustainable agricultural practices. The overreliance on chemical fertilizers has led to environmental degradation, including soil and water pollution. Plant Growth-Promoting Rhizobacteria (PGPR) offer a promising, eco-friendly alternative by enhancing plant growth through various direct and indirect mechanisms. These mechanisms include improving nutrient availability (e.g., via siderophore production, phosphate solubilization, ammonia production), producing phytohormones like auxins (IAA), and mitigating stress through enzymes like ACC deaminase. However, the effectiveness of PGPR is highly dependent on their adaptation to specific local soil, plant, and climatic conditions. While PGPR potentials are well-established globally, a comprehensive profile of native, multifunctional PGPR in the southern Fars province of Iran remains limited. This study aimed to fill this knowledge gap by 1) isolating and evaluating the frequency distribution of key PGP traits among bacterial isolates from various plant rhizospheres, 2) quantitatively assessing selected isolates for IAA production and ACC deaminase activity, and 3) molecularly identifying the most promising multifunctional bacterial strains.




Materials and Methods: A total of 45 rhizosphere samples were collected from diverse plants (including wheat, barley, alfalfa, lettuce, canola, beetroot, and spinach) in southern Fars province, with geographical coordinates recorded via GPS. From these samples, 181 distinct bacterial isolates were obtained through serial dilution (up to 10⁻⁶) and cultivation on Nutrient Agar, TSA, and King B media. All isolates underwent preliminary qualitative screening for five PGP traits: siderophore production (on CAS agar), ammonia production (in peptone water), phosphate solubilization, potassium release, and zinc solubilization. Based on the results of this screening, 12 superior isolates possessing one or multiple strong PGP traits were selected for further quantitative analysis. The quantitative production of IAA was measured spectrophotometrically using Salkowski's reagent in tryptophan-amended broth. The activity of ACC deaminase was estimated by measuring the amount of α-ketobutyrate produced from ACC. Finally, the molecular identification of the top-performing isolates was carried out by sequencing the 16S rRNA gene using universal primers 27F and 1492R, followed by comparison with sequences in the NCBI database.
 
Results: The initial screening of 181 isolates revealed a high diversity of functional traits within the rhizosphere community of Southern Fars. A total of 94 isolates (52%) exhibited at least one plant growth-promoting trait. The analysis of trait frequency indicated that siderophore production and ammonia production were the most prevalent mechanisms, observed in a significant portion of the population. This high prevalence is likely an adaptive response to the iron-limited, alkaline nature of the calcareous soils in the region. Phosphate solubilization and potassium release were also common, whereas zinc solubilization was less frequent but present in highly efficient strains. Multifunctionality: Venn diagram analysis highlighted that while many isolates possessed single traits, a specific subset demonstrated multifunctionality. From this pool, 12 superior isolates were selected for advanced quantitative characterization. All 12 selected isolates demonstrated the capability to synthesize IAA in the presence of tryptophan, with concentrations ranging significantly. The highest IAA production was recorded for isolates SF1050 (52.10 µg/ml) and SF1078 (51.30 µg/ml), with no significant statistical difference between them. This high level of auxin production suggests a strong potential for these strains to stimulate root elongation and increase root surface area. Regarding stress alleviation traits, ACC deaminase activity was observed in 7 out of the 12 isolates. Isolate SF1044 exhibited the highest enzyme activity (306.93 nmol α-ketobutyrate mg⁻¹ protein h⁻¹), followed by SF1038 (292.07 nmol α-ketobutyrate mg⁻¹ protein h⁻¹). The presence of this enzyme indicates the potential of these strains to facilitate plant growth under biotic and abiotic stress conditions by regulating ethylene levels. 16S rRNA sequencing revealed that the elite isolates belonged to five distinct genera: Bacillus, Pseudomonas, Acinetobacter, Pseudarthrobacter, and Lysinibacillus. Specifically, SF1038 was identified as Bacillus sp., and SF1044, SF1050, and SF1092 were identified as Pseudomonas sp. Other isolates were identified as Acinetobacter sp. (SF1075), Pseudarthrobacter sp. (SF1078), and Lysinibacillus sp. (SF1160). The most significant finding was the identification of Pseudomonas sp. SF1044 as a highly versatile, multifunctional strain possessing all seven tested PGP traits simultaneously, including high IAA production and ACC deaminase activity.




Conclusion: This study provides a comprehensive profile of the frequency distribution of PGP traits among the native rhizobacterial community in southern Fars province. The results confirm the presence of a diverse and potent reservoir of PGPR, with a notable prevalence of nutrient-solubilizing bacteria. The isolation and identification of several highly efficient strains, particularly the multifunctional Pseudomonas sp. SF1044, is a significant outcome. This strain, along with other robust isolates like Bacillus sp. SF1038, presents exceptional potential for development into novel, multi-trait biofertilizers. The use of such native, adapted strains can significantly contribute to sustainable agricultural practices in the region by enhancing crop growth and yield while reducing dependence on chemical inputs. Future research should focus on in-vitro and field-level validation of these promising isolates to formulate effective microbial consortia for regional agricultural application.
کلیدواژه‌ها [English]
ACC Deaminase, Frequency distribution, IAA, Molecular identification, PGPR, Rhizosphere
مراجع
  1. Agbodjato, N.A., Noumavo, P.A., Baba-Moussa, F., Salami, H.A., Sina, H., Sèzan, A., Bankolé, H., Adjanohoun, A. and Baba-Moussa, L., 2015. Characterization of potential plant growth promoting rhizobacteria isolated from Maize (Zea mays L.) in central and Northern Benin (West Africa). Applied and Environmental Soil Science, 2015, pp.901656. DOI: 10.1155/2015/901656.
  2. Alexander, D. and Zuberer, D., 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biology and Fertility of Soils, 12(1), pp.39-45.
  3. Asgharzadeh, A., Saghafi, K. and Khoshru, B., 2025. Effect of biopriming with plant growth-promoting bacteria on seed germination indices of wheat under salinity stress. Journal of Soil Biology, 13(1), pp.1-17. (In Persian).
  4. Bhattacharyya, P.N. and Jha, D.K., 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, 28(4), pp.1327-1350. DOI: 10.1007/s11274-011-0979-9.
  5. Cappuccino, J.G. and Sherman, N., 1992. Microbiology: A Laboratory Manual. 3rd Ed., Benjamin/Cummings Pub. Co., New York.
  6. Cappuccino, J.G. and Sherman, N., 2005. Microbiology: A Laboratory Manual. 7th Ed., Pearson/Benjamin Cummings, San Francisco.
  7. Chen, Y., Li, Y., Fu, Y., Jia, L., Li, L., Xu, Z., Zhang, N., Liu, Y., Fan, X. and Xuan, W., 2024. The beneficial rhizobacterium Bacillus velezensis SQR9 regulates plant nitrogen uptake via an endogenous signaling pathway. Journal of Experimental Botany, 75(11), pp.3388-3400. DOI: 10.1093/jxb/eraa123.
  8. Devi, A.R., Kotoky, R., Pandey, P. and Sharma, G., 2017. Application of Bacillus spp. for sustainable cultivation of potato (Solanum tuberosum L.) and the benefits. In: Bacilli and Agrobiotechnology. Springer, Cham, pp.221-243. DOI: 10.1007/978-3-319-44409-3_9.
  9. Foley, J.A., Ramankutty, N., Brauman, K.A., Cassidy, E.S., Gerber, J.S., Johnston, M., Mueller, N.D., O’Connell, C., Ray, D.K. and West, P.C., 2011. Solutions for a cultivated planet. Nature, 478(7370), pp.337-342. DOI: 10.1038/nature10452.
  10. Glick, B.R., 2012. Plant growth‐promoting bacteria: mechanisms and applications. Scientifica, 2012, pp.963401. DOI: 10.6064/2012/963401.
  11. Glick, B.R., 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiological Research, 169(1), pp.30-39. DOI: 10.1016/j.micres.2013.09.009.
  12. Gonin, M., Gensous, S., Lagrange, A., Ducousso, M., Amir, H. and Jourand, P., 2013. Rhizosphere bacteria of Costularia spp. from ultramafic soils in New Caledonia: diversity, tolerance to extreme edaphic conditions, and role in plant growth and mineral nutrition. Canadian Journal of Microbiology, 59(3), pp.164-174. DOI: 10.1139/cjm-2012-0678.
  13. Gupta, S. and Pandey, S., 2019. ACC deaminase producing bacteria with multifarious plant growth promoting traits alleviates salinity stress in French bean (Phaseolus vulgaris) plants. Frontiers in Microbiology, 10, pp.1506. DOI: 10.3389/fmicb.2019.01506.
  14. Ham, S.H., Yoon, A.R., Oh, H.E. and Park, Y.G., 2022. Plant growth-promoting microorganism Pseudarthrobacter sp. NIBRBAC000502770 enhances the growth and flavonoid content of Geum aleppicum. Microorganisms, 10(6), pp.1241. DOI: 10.3390/microorganisms10061241.
  15. Jeon, J.S., Lee, S.S., Kim, H.Y., Ahn, T.S. and Song, H.G., 2003. Plant growth promotion in soil by some inoculated microorganisms. Journal of Microbiology, 41(4), pp.271-276.
  16. Johnson, J.S., Spakowicz, D.J., Hong, B.Y., Petersen, L.M., Demkowicz, P., Chen, L., Leopold, S.R., Hanson, B.M., Agresta, H.O. and Gerstein, M., 2019. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis. Nature Communications, 10(1), pp.5029. DOI: 10.1038/s41467-019-13036-1.
  17. Kang, S.M., Shahzad, R., Bilal, S., Khan, A.L., Park, Y.G., Lee, K.E., Asaf, S., Khan, M.A. and Lee, I.J., 2019. Indole-3-acetic-acid and ACC deaminase producing Leclercia adecarboxylata MO1 improves Solanum lycopersicum L. growth and salinity stress tolerance by endogenous secondary metabolites regulation. BMC Microbiology, 19(1), pp.80. DOI: 10.1186/s12866-019-1450-6.
  18. Khoshru, B., Fallah Nosratabad, A.R., Khosravi, H., Asgharzadeh, A. and Faridian, L., 2025. Enhancing agricultural productivity using PGPR and nanoparticles: mechanisms, challenges, and future directions. Journal of Soil Biology, 12(2), pp.279-313. (In Persian).
  19. Kour, D., Rana, K.L., Yadav, A.N., Yadav, N., Kumar, M., Kumar, V., Vyas, P., Dhaliwal, H.S. and Saxena, A.K., 2020. Microbial biofertilizers: Bioresources and eco-friendly technologies for agricultural and environmental sustainability. Biocatalysis and Agricultural Biotechnology, 23, pp.101487. DOI: 10.1016/j.bcab.2019.101487.
  20. Lahiji, A. and Rejali, F., 2025. Investigating the effect of biofertilizers on improving the absorption of nutrients, growth and yield of hazelnut (Corylus avellana L.) trees in Eshkavrat area of Rudsar city in Gilan province. Journal of Soil Biology, 12(2), pp.213-233. (In Persian).
  21. Payne, S.M., 1994. Detection, isolation, and characterization of siderophores. Methods in Enzymology, 235, pp.329-344. DOI: 10.1016/0076-6879(94)35151-1.
  22. Penrose, D.M. and Glick, B.R., 2003. Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria. Physiologia Plantarum, 118(1), pp.10-15. DOI: 10.1034/j.1399-3054.2003.00086.x.
  23. Rashid, M., Khalil, S., Ayub, N., Alam, S. and Latif, F., 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pakistan Journal of Biological Sciences, 7(2), pp.187-196. DOI: 10.3923/pjbs.2004.187.196.
  24. Salazar-Ramírez, M.T., Preciado-Rangel, P., Fortis-Hernández, M., Rueda-Puente, E.O., Yescas-Coronado, P. and Orozco-Vidal, J.A., 2021. Plant growth-promoting rhizobacteria associated to candelilla rhizosphere (Euphorbia antisyphilitica) and its effects on Arabidopsis thaliana seedlings. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), pp.12294. DOI: 10.15835/nbha49112294.
  25. Saravanan, V.S., Subramoniam, S.R. and Raj, S.A., 2004. Assessing in vitro solubilization potential of different zinc solubilizing bacterial (ZSB) isolates. Brazilian Journal of Microbiology, 35(1-2), pp.121-125. DOI: 10.1590/S1517-83822004000100020.
  26. Savostin, P., 1972. Microbial transformation of silicates. Zeitschrift für Pflanzenernährung und Bodenkunde, 132(1), pp.37-45.
  27. Singh, P., Singh, R.K., Zhou, Y., Wang, J., Jiang, Y., Shen, N., Wang, Y., Yang, L. and Jiang, M., 2022. Unlocking the strength of plant growth promoting Pseudomonas in improving crop productivity in normal and challenging environments: a review. Journal of Plant Interactions, 17(1), pp.220-238. DOI: 10.1080/17429145.2022.2029963.
  28. Somasegaran, P. and Hoben, H.J., 2012. Handbook for Rhizobia: Methods in Legume-Rhizobium Technology. Springer Science & Business Media.
  29. Tafaroji, S.H., Abtahi, S.A., Jafarinia, M. and Ebadi, M., 2022. The effect of plant growth-promoting rhizobacteria producing ACC-Deaminase, IAA, siderophore and phosphate solubilization on growth indices, chlorophyll, proline and protein in alfalfa at different levels of salinity. Plant Productions, 45(3), pp.375-384. (In Persian).
  30. Tan, K.Z., Radziah, O., Halimi, M.S., Khairuddin, A.R., Habib, S.H. and Shamsuddin, Z.H., 2014. Isolation and characterization of rhizobia and plant growth-promoting rhizobacteria and their effects on growth of rice seedlings. American Journal of Agricultural and Biological Sciences, 9(3), pp.342-360. DOI: 10.3844/ajabssp.2014.342.360.
  31. Trivedi, P., Leach, J.E., Tringe, S.G., Sa, T. and Singh, B.K., 2020. Plant–microbiome interactions: from community assembly to plant health. Nature Reviews Microbiology, 18(11), pp.607-621. DOI: 10.1038/s41579-020-0412-1.
  32. Vejan, P., Abdullah, R., Khadiran, T., Ismail, S. and Nasrulhaq Boyce, A., 2016. Role of plant growth promoting rhizobacteria in agricultural sustainability a review. Molecules, 21(5), pp.573. DOI: 10.3390/molecules21050573.
  33. Yuan, Z.S., Liu, F., He, S.B., Zhou, L.L. and Pan, H., 2022. Community structure and diversity characteristics of rhizosphere and root endophytic bacterial community in different Acacia species. PLoS One, 17(1), pp.e0262909. DOI: 10.1371/journal.pone.0262909.
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