| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,593 |
| تعداد مشاهده مقاله | 49,535,995 |
| تعداد دریافت فایل اصل مقاله | 79,946,151 |
Morphological and molecular characterization of Fusarium species causing root and crown rot of safflower | ||
| Mycologia Iranica | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 27 آذر 1404 اصل مقاله (1.65 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2025.370619.1328 | ||
| نویسندگان | ||
| Fatemeh Mostafaei؛ Bahram Sharifnabi* ؛ Zeinab Esmaeili | ||
| Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, IRAN | ||
| چکیده | ||
| Safflower (Carthamus tinctorius) is an annual plant belonging to the family Asteraceae. The cultivation of safflower has gained increasing attention due to its valuable seed oil, vibrant flower color, and tolerance to drought and salinity. However, infections caused by Fusarium species pose a significant challenge worldwide. To investigate the morphological and molecular characteristics of Fusarium species associated with crown and root rot in safflower, samples were collected from infected crowns and roots across various cultivation areas in Isfahan Province. Based on morphological traits and molecular data obtained from sequencing the ITS-rDNA region and a portion of the TEF1 gene, two Fusarium species—F. nygamai, and F. falciforme were identified among 36 isolates exhibiting symptoms of crown and root rot disease. Pathogenicity tests conducted under greenhouse conditions confirmed their pathogenic nature according to Koch’s postulates. Notably, this study represents the first report of F. nygamai and F. falciforme as causal agents of crown and root rot in safflower worldwide. | ||
| کلیدواژهها | ||
| Carthamus tinctorius؛ Disease؛ Fusarium nygamai؛ Fusarium falciforme؛ Pathogenicity | ||
| مراجع | ||
|
Anonymous. 1402. Agricultural Statistics of Crops, Center for Statistics and Information and Communication Technology, Ministry of Agricultural Jihad, Tehran. https://doi.org/ 10.1080/03235408.2016.1275423.
Arif M, Chawla S, Zaidi M, Rayar J, Variar M, Singh U. 2012. Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene. African Journal of Biotechnology. 11(2): 444 –447. doi:10.5897/AJB10.489.
Balmas V, Corda P, Marcello A, Bottalico A. 2000. Fusarium nygamai associated with Fusarium foot rot of rice in Sardinia. Plant Disease 84(7): 807. https://doi.org/10.1094/PDIS.2000.84.7.807B.
Besharati Fard M, Mohammadi A, Drvishnia M. 2017. Fusarium species associated with wheat crown and root tissues in the eastern Iran. Archives of Phytopathology and Plant Protection. 50: 123–133. https://doi.org/10.1080/03235408.2016.1275423.
Bowles VG, Mayerhofer R, Davis C, Good AG, Hall JC. 2010. A phylogenetic investigation of Carthamus combining sequence and microsatellite data. Plant Systematics and Evolution. 287: 85–97. https://doi.org/10.1007/s00606-010-0292-3. Brown DW, Proctor RH. 2013. Fusarium: genomics, molecular and cellular biology. Caister Academic Press, Norfolk, UK.
Cao S, Yang N, Zhao C. Liu J, Han C, Wu X. 2018. Diversity of Fusarium species associated with root rot of sugar beet in China. Journal of General Plant Pathology. 84: 321–329. https://doi.org/10.1007 /s10327-018-0792-5.
Chu L, Li D, Hou W, Dan Z, Wang X, Dong C. 2024. First report of Fusarium falciforme causing root and rhizome rot of Epimedium sagittatum in China. Plant Disease. 108(5): 1400. https://doi.org/10.1094/ PDIS-01-24-0185-PDN.
Da Via D, Knowles, P, Klisiewicz J. 1981. Evaluation of the world safflower collection for resistance to Phytophthora. Crop Science. 21: 226–229. https://doi.org/10.2135/cropsci1981.0011183X002100020005x.
Debbarma R, Maya B, Ashwini JH, Kamil D. 2024. Oligonucleotide-based detection of three plant pathogenic species of Fusarium solani species complex (FSSC) causing tomato root rot and wilt disease. Physiological and Molecular Plant Pathology. 134: 102478. https://doi.org/10.1016/ j.pmpp.2024.102478.
Divakara ST, Santosh P, Aiyaz M, Ramana MV, Hariprasad P, Nayaka SC, Niranjana SR. 2014. Molecular identification and characterization of Fusarium spp. Associated with sorghum seeds. Journal of the Science of Food and Agriculture. 94(6): 1132-1139. https://doi.org/10.1002/jsfa.6380.
Ekwomadu TI, Mwanza, M. 2023. Fusarium fungi pathogens, identification, adverse effects, disease management, and global food security: a review of the latest research. Agriculture. 13(9): 1810. https://doi.org/10.3390/agriculture13091810.
Emongor V, Oagile O, Phuduhudu D, Oarabile P. 2017. Safflower production. Botswana University of Agriculture and Natural Resources. Gaborone.
Eslahi MR. 2012. Fungi associated with root and crown rot of wheat in Khuzestan province, Iran. Journal of Crop Protection. 1(2):107–113.
Esmaili Z, Sharifnabi, B. 2023. Fusarium species associated with apple trees decline in Isfahan, Iran. Mycologia Iranica. 10(1): 23–34. 10.22092/ MI.2022.360249.1235.
FAOSTAT 2022. Food and Agriculture Organization of the United Nations. Internet resource: http://Faostat.Fao.org.
Fisher NL, Burgess LW, Toussoun TA, Nelson PE, 1982. Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology. 72(1): 151.
Garmaroodi HS, Jabbari H, Nazari M, Valipour M. 2024. Assessment of genetic resistance of safflower (Carthamus tinctorius) to Fusarium damping off in greenhouse conditions. Journal of Iranian Plant Protection Research. 38(1): 35–47. https://doi.org /10.22067/jpp.2024.86136.1172.
Geiser, DM, del Mar Jimenez-Gasco M, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, O’Donnell K. 2004. FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology. 110: 473–479. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0.
Gouy M, Guindon Ph, Gascuel O. 2010. SeaView Version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution. 27: 221–224.
Habibi A, Mansouri SM, Sadeghi B. 2018. Fusarium species associated with medicinal plants of Lamiaceae and Asteraceae. Mycologia Iranica. 5(2): 91–101. doi: 10.22043/mi.2019.120383.
Hussain MI, Lyra DA, Farooq M, Nikoloudakis N, Khalid N. 2016. Salt and drought stresses in safflower: a review. Agronomy for Sustainable Development. 36(4):1–31. https://doi.org/10.1007/ s13593-015-0344-8.
Jangir P, Singh N, Goel S, Kapoor R. 2023. Emergence of wilt disease caused by Fusarium proliferatum in safflower-growing fields in India. Journal of Plant Pathology. 105: 1103–1108. https://doi.org/10.1007/ s42161-023-01452-8.
Li W, Yoo E, Sung J, Lee S, Hwang S, Lee GA. 2023. Distinct effects of seed coat and flower colors on metabolite contents and antioxidant activities in safflower seeds. Antioxidants. 12(4): 961. https://doi.org/10.3390/antiox12040961.
Kim SG, Ko HC, Hur OS, Luitel BP, Rhee JH, Yoon MS, Baek HJ, Ryu KY, Sung JS. 2016. First report of Fusarium wilt caused by Fusarium proliferatum on safflower. Research in Plant Disease. 22(2): 111–115. https://doi.org/10.5423/RPD.2016.22.2.111.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 33(7): 1870–1874. https://doi.org/ 10.1093/ molbev/msw054.
Kurmut AM, Nirenberg HI, Bochow H, Buttner C. 2002. Fusarium nygamai, a casual agent of root rot of Vicia faba L. in the Sudan. Mededelingen-Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen. Universiteit Gent 67(2): 269–274.
Leslie J, Summerell B. 2006. The Fusarium Laboratory Manual. Blackwell, USA.
Ma LJ, Geiser DM, Proctor RH, Rooney AP, O’Donnell K, Trail F, Gardiner DM, Manners JM, Kazan K. 2013. Fusarium Pathogenomics. Annual Review of Microbiology. 67: 399–416. https://doi.org/10.1146/ annurev-micro-092412-155650.
Maghsoudlou R, Taheri AAH, Rahnema K. 2007. Identification and pathogenicity of Fusarium spp. Isolated from root and crown of wheat in Gorgan area. Journal of Agricultural Sciences and Natural Resources. 14(2): 175–189.
Mehdinia F, Darvishnia M, Mirzaei Najafgholi H, Nazarian-Firouzabadi F. 2024. Study of Fusarium species causing wilt and root rot in chickpeas in Lorestan province. Plant Protection. 47(3): 101–116. 10.22055/ppr.2024.48286.1774.
Minati MH. 2020. First record of nine Fusarium spp. causing root rot on wheat (Triticum aestivum L.) in Iraq. AIP Conference Proceedings 2290: 020009. https://doi.org/10.1063/5.0027398.
Murray M, Thompson WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 8(19): 4321–4325. https://doi.org/ 10.1093/ nar/8.19.4321.
Nelson PE, Toussoun TA, Marasas W. 1983. Fusarium species: an illustrated manual for identification. Penn State University Press. United States of America.
Nelson PE, Dignani MC, Anaissie EJ. 1994. Taxonomy, biology, and clinical aspects of Fusarium species. Clinical Microbiology Reviews. 7(4): 479–504. https://doi.org/10.1128/cmr.7.4.479.
O’Donnell K, Nirenberg HI, Aoki T, Cigelnik E. 2000. A multigene phylogeny of the Gibberella fujikuroi species complex: detection of additional phylogenetically distinct species. Mycoscience. 41(1): 61–78. https://doi.org/10.1007/BF02464387.
Prasad R, Navaneetha T, Nageswara Rao N. 2011. Cultural, morphological, pathogenic, and molecular diversity in Macrophomina phaseolina isolates of safflower from southern India. Indian Phytopathological Society 64(3): 247–253.
Qostal S, Kribel S, Mouden N. Chliyeh M, El Alaoui MA, Selmaoui K, Benkirane R, Touhami AO, Douira A, 2025. Fusarium falciforme, another causal agent of root rot of wheat and barley varieties in Morocco. 3 Biotech 15: 165. https://doi.org/ 10.1007/s13205-025-04333-2.
Raghuwanshi K, Dake G. 2009. Fusarium wilt of safflower (Carthamus tictorius L.): A review. Journal of Plant Disease Sciences. 4: 110–117.
Rajakumara A, Bhat G, Shivakumar G, Madalageri BB, Noojibail P, Anandalakshmi R. 2024. Fusarium brachygibbosum and Fusarium nygamai cause basal rot of onion (Allium cepa) in India. New Disease Reports. 50(2): e12311. https://doi.org/ 10.1002/ndr2.12311.
Rauf CA, Rafique K, Naz F, Kang S. 2016. First report of lentil wilt caused by Fusarium nygamai in Pakistan. Journal of Plant Pathology 98(3): 696.
Sabahi F, Banihashemi Z, Mirtalebi M, Rep M, Cacciola SO. 2023. Molecular variability of the Fusarium solani species complex associated with Fusarium wilt of melon in Iran. Journal of Fungi. 9(4): 486. https://doi.org/10.3390/jof9040486.
Safaee DH, Younesi H, Sheikholeslami M. 2012. Fusarium species that cause root and crown rot of wheat in Kermanshah province. Iranian Journal of Plant Patholog.y 48(2): 265–268.
Silvia SGA, Costa MM, Cardoso AMA, Nascimento LV, Barroso KA, Nunes GHS, Pfenning LH, Ambrósio MMQ. 2023. Fusarium falciforme and Fusarium suttonianum cause root rot of melon in Brazil. Plant Pathology. 72(4): 721–730. https://doi.org/10.1111/ppa.13701.
Summerell BA, Salleh B, Leslie JF. 2003. A Utilitarian Approach to Fusarium Identification. Plant Disease 87(2): 117–128. https://doi.org/10.1094/PDIS. 2003. 87.2.117.
Taware M, Gholve V, Dey U. 2014. Bio-efficacy of fungicides, bioagents, and plant extracts/botanicals against Alternaria carthami, the causal agent of Alternaria blight of Safflower (Carthamus tinctorius L.). African Journal of Microbiology Research. 8(13): 1400–1412. https://doi.org/ 10.5897/ AJMR 2013
Tirado-Ramírez M, López-Orona C, López-Billard J, Molina-Cárdenas L, Sánchez-Rangel J, Amarillas-Bueno L, Vega-Gutiérrez T. 2023. First report of Fusarium verticillioides causing safflower root rot in Sinaloa, Mexico. Plant Disease. 107(9): 2851. https://doi.org/10.1094/PDIS-02-23-0257-PDN.
White TL, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Innis, MA, Gelfand, DH, Sninsky, JJ, White, TJ (Eds.). Academic Press, pp 315–322.
Zhang N, Zhu YN, Wei ZX, Gou JJ, Gao HJ, Yang WX. 2023. First Report of Fusarium nygamai causing crown rot of wheat in China. Plant Disease. 107(7): 2262. https://doi.org/10.1094/PDIS-12-22-2844-PDN.
| ||
|
آمار تعداد مشاهده مقاله: 5 |
||