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Karyoevolutive consideration of two sea fish species via the newest short-term culture method | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 24، شماره 6، بهمن 2025، صفحه 1477-1493 اصل مقاله (1017.01 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2025.134653 | ||
| نویسندگان | ||
| S. Saygun* 1؛ M. Gundogan2 | ||
| 1Ordu University, Faculty of Marine Science, Fishery Technology Engineering, Department of Aquaculture, Fatsa, Ordu, Turkey | ||
| 2Ordu University, Institute of Science, Ordu, Turkey | ||
| چکیده | ||
| This study successfully determined the chromosome numbers and karyotypes of two marine fish species from separate families using the short-term culture method (PB-MAX™ application). The culture incubation periods varied for each species. Kidney tissues from the fish samples were treated with PB-MAX™ for either 2.5 or 4 h. Results showed that diploid chromosome numbers, karyotypes, and arm numbers differ by species: Red Mullet, Mullus barbatus, has 2n – 44, with 3 pairs of metacentric, 7 pairs of submetacentric, 7 pairs of subtelocentric, and 5 pairs of acrocentric chromosomes (NF=64). Round Goby, Neogobius melanostomus, has 2n – 46, consisting of 46 pairs of acrocentric chromosomes (NF=46). C– and NOR–positive results were achieved through constitutive heterochromatin banding and silver nitrate staining for both species. The primary objective of this research is to demonstrate the applicability of short-term culture in producing successful cytogenetic results in marine fish. This study will support future research and facilitate chromosome studies on marine fish, making the process quick and practical. | ||
| کلیدواژهها | ||
| Marine fishes؛ Mullus barbatus؛ Neogobius melanostomus؛ Chromosome؛ Short-term culture | ||
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آمار تعداد مشاهده مقاله: 163 تعداد دریافت فایل اصل مقاله: 56 |
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