| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,079 |
| تعداد مقالات | 34,323 |
| تعداد مشاهده مقاله | 47,433,083 |
| تعداد دریافت فایل اصل مقاله | 78,393,394 |
تاثیر مخرب فرمولاسیون نانو روغن چریش، Azadirachta indica، روی ملخ صحرایی، Schistocerca gregari | ||
| نامه انجمن حشره شناسی ایران | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 مهر 1404 | ||
| نوع مقاله: مقاله کامل، انگلیسی | ||
| شناسه دیجیتال (DOI): 10.22117/jesi.2025.368826.1713 | ||
| نویسندگان | ||
| نادیا دیمتری1؛ محفوظ عبد الجواد2؛ دالیا یوسف* 3؛ سمیرا نابل4 | ||
| 1بخش آفات و حفاظت از گیاهان، مرکز تحقیقات ملی، دوکی،جیزه، مصر | ||
| 2بخش بیماری‌شناسی گیاهی، مرکز تحقیقات ملی، دوکی،جیزه، مصر | ||
| 3بخش آفات و حفاظت از گیاهان، مرکز تحقیقات ملی، دوکی،جیزه، مصر و موسسه تحقیقات بیوتکنولوژی گیاهی، مهندسی ژنتیک و بیوتکنولوژی، دانشگاه شهر سادات، مصر | ||
| 4بخش تحقیقات ملخ، موسسه تحقیقات حفاظت از گیاهان، مرکز تحقیقات کشاورزی، جیزه، مصر | ||
| چکیده | ||
| آفت‌کش‌های زیستی گیاهی به دلیل اثربخشی‌شان در مبارزه با انواع آفات زراعی شناخته شده‌اند. اهمیت این آفت‌کش‌ها، قابلیت تجزیه زیستی و مقرون‌به‌صرفه بودنشان می‌باشد. کاربرد فناوری نانو به عنوان راهی امیدوارکننده برای پیشرفت روش‌های کنترل آفات در نظر گرفته می‌شود. این مطالعه، تأثیر مخربی را در ارتباط با تبدیل روغن چریش به شکل نانو با استفاده از امواج فراصوت پرانرژی نشان داد. تجزیه و تحلیل میکروسکوپ الکترونی عبوری (TEM) نشان داد که نانوذرات تهیه شده، کروی و صاف بودند و میانگین اندازه ذرات آنها از 90 تا 120 نانومتر متغیر بود. اثرات روغن چریش فله‌ای و نانوامولسیون آن بر روی پوره‌های سن پنجم ملخ صحرایی، Schistocerca gregaria در شرایط نیمه‌مزرعه ارزیابی شد. کاربرد موضعی هر دو فرمولاسیون، در دوزهای 2.5 و 5 ppm، منجر به افزایش قابل توجه میزان مرگ و میرو همچنین ناهنجاری‌های پورگی و پوست‌اندازی ناموفق، در مقایسه با گروه کنترل شد. با این حال، فرم خام روغن چریش، اثربخشی بیشتری نسبت به فرمولاسیون نانو نشان داد. فعالیت کیتیناز و پروتئین کل تحت تأثیر هر دو فرمولاسیون قرار گرفتند. نکته قابل توجه این است که نانو امولسیون منجر به افزایش قابل توجه مدت زمان زنده ماندن پوره‌ها شد. تجزیه و تحلیل کروماتوگرافی گازی/طیف‌سنجی جرمی (GC/MS)، عدم وجود اسید لینولئیک در نمونه نانو را نشان داد، که ممکن است دلیل اثربخشی برتر نمونه خام نسبت به فرمولاسیون نانو باشد. تیمار اولتراسونیک طولانی مدت در طول تهیه نانو امولسیون روغن چریش، بر ترکیبات زیستی فعال موجود در روغن چریش تأثیر منفی گذاشت و منجر به کاهش فعالیت زیستی آن درروی پوره‌های سن پنجم S. gregaria در مقایسه با فرم خام آن شد. | ||
| کلیدواژه‌ها | ||
| Nanotechnology؛ Pest control؛ Azadirachta indica؛ Schistocerca gregaria؛ Plant extract | ||
| عنوان مقاله [English] | ||
| The adverse impact of transforming neem oil (Azadirachta indica) into a nano formulation for application as a botanical pesticidal agent against the desert locust Schistocerca gregaria | ||
| نویسندگان [English] | ||
| Nadia Dimetry1؛ Mahfouz Abd-Elgawad2؛ Dalia Youssef3؛ Samira Nabel4 | ||
| 1Pests and Plant Protection Department., National Research Centre, Dokki 12622, Giza, Egypt | ||
| 2Plant Pathology Department., National Research Centre, Dokki 12622, Giza, Egypt | ||
| 3Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt | ||
| 4Locust and Grasshoppers Research Department, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt | ||
| چکیده [English] | ||
| Botanical biopesticides are recognized for their efficacy in combating a variety of crop pests. They are characterized by their biodegradability and affordability. The application of nanotechnology is viewed as a promising avenue for advancing pest control methods. This study revealed a detrimental impact associated with the conversion of neem oil into its nano form using high-energy sonication. Transmission Electron Microscopy (TEM) analysis indicated that prepared nano particles were spherical and smooth with mean particle size ranged from 90 to 120 nm. The effects of both bulk neem oil and its nano-emulsion were evaluated against the fifth instar nymphs of Schistocerca gregaria under semi-field conditions. Topical applications of both formulations, at doses of 5 and 2.5 ppm, resulted in a significant increase in mortality rates, as well as nymphal malformations and failed molting, in comparison to the control group. However, the crude form of neem oil demonstrated greater effectiveness than the nano formulation. The chitinase activity and total protein were influenced by both formulations. Notably, the nano emulsion resulted in a significant extension of duration period in the survived nymphs. Gas chromatography/mass spectrometry (GC/MS) analysis indicated the absence of linoleic acid in the nano sample, which may account for the superior efficacy of the bulk sample over the nano formulation. The prolonged ultrasonic treatment during preparation of neem oil nano-emulsion adversely affected the active biocompounds presented in neem oil, leading to a reduction in its bioactivity against the fifth instar nymphs of S. gregaria compared to its crude form. | ||
| کلیدواژه‌ها [English] | ||
| Nanotechnology, Pest control, Azadirachta indica, Schistocerca gregaria, Plant extract | ||
| مراجع | ||
|
Abbott, W. S. (1925) A method for computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2):265-267.
Abdel-Aal, A. E. (2006) Effect of chlorfluazuron, nuclear polyhydrosis virus (SLNPV) and Bacillus thuringiensis on some biological and enzymes activity of cotton leafworm, Spodoptera littoralis (Boisd). Bulletin of the Entomological Society of Egypt (ARE), Economic Series 32, 171-185.
Al-Maroug, N. A., A A, Nassar, M. I., Abdelatef, G. M., ELShazly, M. M., Abd ElFattah, E. A. E. & Abd El-Monem, D. H. (2022) Assessment of the commercial botanical insecticides against the desert locust, Schistocerca gregaria (Forsk.) (Orthoptera: Acrididae). Egyptian Academic Journal of Biological Sciences F. Toxicology and Pest Control, 14(2):121-132. https://doi.org/10.21608/eajbsf.2022.266750
Al-shannaf, H. M., Mead, H. M. & Sabry, A. H. (2012) Toxic and biochemical effects of some bioinsecticides and IGRs on american bollworm, Helicoverpa armigera (Hüb.) (Noctuidae: Lepidoptera) in cotton fields. Journal of Biofertilizers and Biopesticides, 3(2):1-6. http://dx.doi.org/10.4172/2155-6202.1000118
Al-Sharook, Z., Balan, K., Jiang, Y. & Rembold, H. (1991) Insect growth inhibitors from two tropical Meliaceae: effect of crude seed extracts on mosquito larvae. Journal of Applied Entomology, 111:425- 430. https://doi.org/10.1111/j.1439-0418.1991.tb00344.x
Amin, A. H., Bayoumi, A. E., Dimetry, N. Z. & Youssef, D. A. (2019) Efficiency of Nano-formulations of neem and peppermint oils on the bionomics and enzymatic activities of Agrotis ipsilon larvae (Lepidoptera: Noctuidae). International Journal of Natural Resource Ecology and Management, 4(5):102-111. https://doi: 10.11648/j.ijnrem.20190405.11
Bashir, E. M. & El Shafie, H. A. (2014) Toxicity, antifeedant and growth regulating potential of three plant extracts against the desert locust Schistocerca gregaria Forskal (Orthoptera: Acrididae). American Journal of Experimental Agriculture, 4(8): 959-970.
Bayoumi, A. E., Amin, A. H., Dimetry, N. Z. & Youssef, D. A. (2022) Toxicity evaluation of two Neem oil nano- Formulations using Swiss male albino mice. Biomedical Journal of Science and Technical Research, 43(4):34763-34777. https://doi:10.26717/BJSTR.2022.43.006928
Bogumil, S. G. L. & Wieslaw, O. (2006) Effect of low and high-saponin lines of alfalfa on pea aphid. Journal of Insect Physiology, 52(7), 737-743. https://doi.org/10.1016/j.jinsphys.2006.04.001
Cullen, D.A., Cease, A.J., Latchininsky, A. V., Ayali, A., Berry, K., Buhl, J., Keyser, R. De, Foquet, B., Hadrich, J.C., Matheson, T., Ott, S.R., Poot-Pech, M.A., Robinson, B.E., Smith, J.M., Song, H., Sword, G.A., VandenBroeck, J, Verdonck, R., Verlinden, H., Rogers, S.M., 2017. From molecules to management: mechanisms and consequences of locust phase polyphenism. In Advances in Insect Physiology 53:167-285. Academic Press. https://doi.org/10.1016/bs.aiip.2017.06.002
Da Cruz, R. C. D., da Silva Carvalho, K., Costa, R. J. O., da Silva, P. A., e Silva, S. L. D. C., Gualberto, S. A., de Gusmão N. B. & de Souza, I. A. (2020) Phytochemical and toxicological evaluation of a blend of essential oils of Croton species on Aedes aegypti and Mus musculus. South African Journal of Botany, 132, 188-195. https://doi.org/10.1016/j.sajb.2020.03.040
De Geyter, E., Swevers, L., Soin, T., Geelen, D. & Smagghe, G. (2012) Saponins do not affect the ec-dysteroid receptor complex but cause membrane permeation in insect culture cell lines. Journal of Insect Physiology, 58(1):18-23. https://doi.org/10.1016/j.jinsphys.2011.09.005
Despland, E. & Simpson, S. J. (2000) The role of food distribution and nutritional quality in behavioural phase change in the desert locust. Animal Behaviour 59(3): 643–652. https://doi.org/10.1006/anbe.1999.1335
Dimetry, N. Z., Amin, A. H., Bayoumi, A. E., Aly, M.A. & Youssef D. A. (2019) Comparative toxicity between neem and peppermint oils nano-formulations against Agrotis ipsilon (Hufm.) larvae (Lepidoptera: Noctuidae). Jounal of Botanical Research, Bilingual Publishing Co. 1:13-19.https://doi.org/10.30564/jrb.v1i1.590
Disha Varijakzhan, Chou-Min Chong, Kok-Song Lai & Swee-Hua Erin Lim (2020) Middle Eastern plant extracts: An alternative to modern medicine problems. Molecules, 25(5):1126. https://doi.org/10.3390/molecules 25051126.
El-Shazly, M. M., Ghazawy, N. A. R., Abdel Rahman, K. M., Elbassiony G. M. & Nassar, M. I. (2019) Tolerance of Heteracris littoralis (Orthoptera: Acrididae) to the toxic plant, Nerium oleander (Apocynacea). Journal of the Egyptian Society of Parasitology, 49(3):579-587. https://doi.org/10.21608/jesp.2019.68061
El-Sonbati, A. Z., Diab, M. A., El-Bindary, A. A., Nozha, S.G. & Nassar, M. I. (2014) Evaluation and rearrangement of novel supramolecular 7-(2,3-Dihydro-1,3- benzothiazol-2-yl) quinolin-8-ol complexes and their biological effect. International Journal of Microbial and Biochemical Technology S4-001, 2-9. http://dx.doi.org/10.4172/1948-5948.S4-001.
FAO (2001) P.M. Symmons and K. Cressman Desert Locust Guidelines Biology and behaviour Second edition. https://www.fao.org/home/
FAO (2020) FAO Feature Story. Five things you should know about an age-old pest: the desert locust. https://www.fao.org/newsroom/story/Five-things-you-should-know-about-an-age-old-pest-the-Desert-Locust/en
Gelbic, I. & Ne´mec, V. (2001) Developmental changes caused by metyrapone and azadirachtin in Spodoptera littoralis (Boisd.) (Lep.,Noctuidae) and Galleria mellonella (L.) (Lep., Pyralidae). Journal of Applied Entomology 125(7):417–422. https://doi.org/10.1046/j.1439-0418.2001.00566.x
Gornall, A. G., Bardawill, C. J. and David, M. M. (1949) Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry, 177(2):751-766.
Hamadah, Kh. Sh., Ghoneim, K. S., El-Hela, A. A., Amer, S. M. and Mohammad, A. A. (2013) Disturbed survival, growth and development of the desert locust Schistocerca gregaria by different extracts of Azadirachta indica (Meliaceae) and Nigella sativa (Ranunculaceae). Egyptian Academic Journal of Biological Sciences A. Entomology. 6(2):1-21. Doi: 10.21608/eajbsa.2013.13246
Ishaaya, I. & Casida, J. E. (1974) Dietary TH 6040 alters composition and enzyme activity of housefly larval cuticle. Pesticide Biochemistry and Physiology, 4(4):484-490. https://doi.org/10.1016/0048-3575(74)90073-X
Jerobin, J., Sureshkumar, R. S., Anjali, C.H., Mukherjee, A. & Chandrasekaran N. (2012) Biodegradable polymer based encapsulation of neem oil nanoemulsion for controlled release of Aza-A. Carbohydrate Polymers, 90:1750-1756.
Josephrajkumar, A., Subrahmanyam, B. and Srinivasan, S. (1999) Plumbagin and azadirachtin deplete haemolymph ecdysteroid levels and alter the activity profiles of two lysosomal enzymes in the fat body of Helicoverba armigera (Lepidoptera: Noctuidae). European Journal of Entomology, 96(4):347- 353.
Kumar, S., Bhanjana, G., Sharma, A., Sidhu, M. C. and Dilbaghi, N. (2014) Synthesis, characterization and on field evaluation of pesticide loaded sodium alginate nanoparticles. Carbohydrate Polymers, 101:1061-1067. https://doi.org/10.1016/j.carbpol.2013.10.025
Lertsutthiwong, P., Noomun, K., Jongaroonngamsang, N., Rojsitthisak, P. and Nimmannit, U. (2008) Preparation of alginate nanocapsules containing turmeric oil, Carbohydrate Polymers, 74(2):209-214. https://doi.org/10.1016/j.carbpol.2008.02.009
Linton, Y. M., Nisbet, A. J. & Mordue, A. J. (1997) The effects of azadirachtin on the testes of the desert locust, Schistocerca gregaria Forskål. Journal of Insect Physiology, 43(11):1077-1084. https://doi.org/10.1016/S0022-1910(97)00060-7
Liu, X. C., Li, Y. P., Li, H. Q., Deng, Z. W., Zhou, L., Liu, Z. L. and Du, S. S. (2013) Identification of repellent and insecticidal constituents of the essential oil of Artemisia rupestris L. aerial parts against Liposcelis bostrychophila Badonnel. Molecules 18(9):10733–10746. https://doi.org/10.3390/molecules180910733
Lomer, C. J., Bateman, R. P., Johnson, D. L., Langewald, J. and Thomas, M. (2001) Biological control of locusts and grasshoppers. Annual Review of Entomology, 46(1):667-702. https://doi.org/10.1146/annurev.ento.46.1.667
Mahmoud, D. M., Elnaggar, T. F., Fattah H. M. A. & Kader, A. F. A. (2014) Effect of azadirachtin on the hemolymph protein of the adult desert locust, Schistocerca gregaria (Forskal) (Orthoptera: Acrididae). Egyptian Journal of Pure and Applied Science, 52(2):1-5.
Mohammad, A. A., Amer, H. M., El-Sawy, S. M., Youssef, D. A., Nour, S. A. & Soliman, G. M. (2022) Nematicidal activity of sweet annie and garden cress nano-formulations and their impact on the vegetative growth and fruit quality of tomato plants. Scientific Reports, 12(1):22302. https://doi.org/10.1038/s41598-022-26819-2
Nassar, M. I, Ghazawy, N. A., Torkey, H. M. & Rawy, S. M. (2018) Assessment of biorational botanical extracts on the Desert Locust Schistocerca gregaria Forskal (Orthoptera: Acrididae). Entomology and Applied Science Letters, 5(2):42-54.
Nicol, C. M. Y. & Schmutterer, H. (1991) Contact effects of seed oil from the neem tree Azadirachta indica (A. Juss.), on nymphs of the gregarious phase of the desert locust, Schistocerca gregaria (Forsk.). Journal of Applied Entomology, 111(2):197 –205. https://doi.org/10.1111/j.1439-0418.1991.tb00311.x
Pener, M. P. & Simpson, S. J. (2009) Locust phase polyphenism: an update. Advances in Insect Physiology, 36,1–272. https://doi.org/10.1016/S0065-2806(08)36001-9
Reda, F. A. Mona, I. M., El-Gammal, M. & Noura, M. M. (2009) Biochemical changes of the Chitin-synthesis inhibitor, Hexaflumuron (Consult) compound on the desert locust, Schistocerca gregaria (Forskal). Egyptian Academic Journal of Biological Sciences A, 1(1):59 – 68.
Ouedraogo, R. M., Kamp, A., Goettel, M. S., Brodeur, J. & Bidochka, M. J. (2002) Attenuation of fungal infection in thermo regulating Locusta migratoria is accompanied by changes in heamolymph proteins. Journal of Invertebrate Pathology, 81(1):19-24. https://doi.org/10.1016/S0022-2011(02)00117-9
Schmutterer, H. & Frekes, T. (1990) Beeinflussung von Metamorphose, Farbung und Verhalten der Wiisten-heuschrecke Schistocercu gregaria (Forsk.) under Afrikanischen Wanderheuschrecke Locusta migrutoria migratortorioides (R. & F.) durcli Niemsamcniil. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 97(4):431-438.
Sugumaran, M. (2010) Chemistry of cuticular sclerotization. Advances in Insect Physiology. 39:151-209. https://doi.org/10.1016/B978-0-12-381387-9.00005-1
Sword, G. A., Simpson, S. J., El Hadi, O. M. & Wilps, H. (2000) Density–dependent aposematism in the desert locust. Proceedings of The Royal Society B, London 267:63–68. https://doi.org/10.1098/rspb.2000.0967
Van Huis, A., Cressman, K. & Magor, J. I. (2007) Preventing desert locust plagues: Optimizing management interventions. Entomologia Experimentaliset Applicata, 122(3):191-214. https://doi.org/10.1111/j.1570-7458.2006.00517.x
Youssef, D. A., Bayoumi, A. E., Dimetry, N. Z., Amin, A. H. & Hoballah, E.M. (2018) Evaluating effect of peppermint oil (Mentha pipreta) and its nano-formulations on some enzymatic activities and bionomics of cotton leaf worm Spodoptera littoralis (Biosd.). Arab Universities Journal of Agricultural Sciences: Special Issue, 26 (2c), 1977-1991. https://doi: 10.21608/ajs.2018.31665
Youssef, D. A. & Abdelmegeed, S. M. (2021) Polymer-based encapsulation of peppermint oil (Mentha piperita) nano emulsion and its effects on life and some physiological activities of honeybees (Hymenoptera). Egyptian Pharmaceutical Journal, 20(4):313-322. https://doi.org/10.4103/epj.epj_49_21
Zhang, Y., Zhu, S., Yin, L., Qian, F., Tang, C. &Yin, C. (2008) Preparation, characterization and biocompatibility of poly (ethylene glycol)-poly (n-butyl cyanoacrylate) nano capsules with oil core via mini emulsion polymerization. European Polymer Journal, 44(6):1654–1661. https://doi.org/10.1016/j.eurpolymj.2008.03.019 | ||
|
آمار تعداد مشاهده مقاله: 11 |
||