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بهینهسازی تولیدترکیب ضد قارچی سورفکتین در Bacillus velezensis UTB96 | ||
| آفات و بیماری های گیاهی | ||
| دوره 93، شماره 1 - شماره پیاپی 120، شهریور 1404، صفحه 13-25 اصل مقاله (692.12 K) | ||
| نوع مقاله: مدیریت آفات و بیماریهای گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2025.366438.1522 | ||
| نویسندگان | ||
| مسعود احمدزاده* 1؛ مائده احمدزاده2؛ ملیحه وحیدی نسب3 | ||
| 1کرج 'گروه گیاه پزشکی دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران | ||
| 2گروه گیاه پزشکی دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران | ||
| 3انستیتو علوم غذایی و بیوتکنولوژی | ||
| چکیده | ||
| سورفکتین قدرتمندترین و یکی از مهمترین لیپوپپتیدهای حلقوی است که توسط Bacillus velezensis و برخی گونههایBacillus دیگر تولید میشود و کاربرد وسیعی در صنعت، پزشکی، داروسازی و کنترل زیستی آفات و بیماریهای گیاهی در کشاورزی دارد. به منظور افزایش تولید لیپوپپتیدهای حلقوی، انتخاب محیط کشت مناسب و اقتصادی و مهیاکردن بهترین شرایط محیطی تولید، اهمیت زیادی دارد. سویه B. velezensis UTB96 با تولید لیپوپپتیدهای حلقوی موجب کاهش کشش سطحی آب از mN/m 72 به mN/m 30 شد. در این پژوهش، دو محیط کشت متفاوت تهیه شد و میزان تولید سورفکتین با استفاده از HPLC در زمانهای 24، 48 و 72 ساعت پس از مایهزنی، در دو دمای 25 و 37 درجه سلسیوس و دو محیط متفاوت از نظر منابع نیتروژن و برخی فلزات مؤثر محاسبه شد. نتایج نشانداد که بیشترین میزان تولید سورفکتین مربوط به دمای 37 درجه سلسیوس، طول دوره رشد 48ساعته و با منبع نیتروژن نیترات آمونیوم و غلظت 01/0 میلیمول سولفات منگنز و چهار میکرومول سولفات آهن است. بهطور کلی، نتایج این پژوهش نشان داد که سویۀ B. velezensis UTB96توان بالقوهای در تولید لیپوپپتید حلقوی سورفکتین دارد و بهینهسازی محیط کشت و عوامل محیطی تاثیر معنیداری بر افزایش میزان تولید سورفکتین دارد؛ لذا این سویه را میتوان برای کاربردهای صنعتی و تولید تجاری سورفکتین پیشنهاد کرد. همچنین، لیپوپپتیدهای حلقوی خالصسازی شده از این سویه در غلظت یک میلیگرم بر میلیمول توانستند از رشد قارچ Aspergillus flavus جلوگیری کنند. | ||
| کلیدواژه‌ها | ||
| آسپرژیلوس؛ بیوسورفکتانت؛ خواص ضد میکروبی؛ سورفکتین؛ لیپوپپتید حلقوی | ||
| عنوان مقاله [English] | ||
| Optimization of antifungal metabolite surfactin production by Bacillus velezensis UTB96 | ||
| نویسندگان [English] | ||
| Masoud Ahmadzadeh1؛ Maedeh Ahmadzadeh2؛ Malihe Vahidi nasab3 | ||
| 1Dept. of Plant Protection, College University of Agriculture and Natural Resources, University of Tehran | ||
| 2Dept. of Plant Protection, College University of Agriculture and Natural resources, University of Tehran | ||
| 3Institute of Food Science and Biotechnology. University of Hohenheim | ||
| چکیده [English] | ||
| Surfactin is one of the most effective bacterial cyclic lipopetides mainly produced by different species of Bacillus that has antifungal activity. This compound are considered for using in various industries, medical and pharmaceutical and agricultural as an effective antibiotic for pest and disease management. Mostly the production of secondary metabolites in microorganisms is strongly influenced by physical and chemical conditions. In the current study, effect of some factors on surfatin production of B. velezensis UTB96 were evaluated. This strain is able to reduce the surface tension of the water from 72 mN/m to 30 mN/m. In this research we evaluated the production of surfactin by B. velezensis UTB96 as a potent producer. The strain was cultured in two different media. The effect of different incubation time including 24, 48 and 72 hours, different temperature including 25 and 37 ˚C, and also, different sources of nitrogen and finally different concentration of microelements surfactin production has been evaluated using HPLC analysis. HPLC analysis indicated that surfactin considerably produced at the optimum temperature of 37˚C for a 48h-incubation period. Regarding media components, it was found that the optimum nitrogen source was NH4NO3 (50 mM) and also, the optimum mineral slats were MnSO4 (0.01 mM), FeSO4 (4 uM). These results tend to suggest that B. velezensis UTB96 might be an appropriate strain to produce surfactin in bioreactor fermentation for large-scale production in order to apply for controlling A. flavus and to reduce its dangerous mycotoxin, aflatoxin as well. It was also revealed that the extracted cyclic lipopeptides could inhibit the growth of the fungus Aspergillus flavus at the concentration of 1mg/ml. | ||
| کلیدواژه‌ها [English] | ||
| Antimicrobials, Aspergillus, biosurfactant, cyclic lipopeptid, surfactin | ||
| مراجع | ||
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