اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,081
تعداد مقالات34,371
تعداد مشاهده مقاله47,878,276
تعداد دریافت فایل اصل مقاله78,877,416
Li, K.S., Zhu, Y.M., Zhou, X., Hu, Y.P., Ju, C.Z., Wang, Y.T., Hu, M.J., Zhang, C., Zhang, J.Q.. (1404). Molecular characterization of cyprinid herpesvirus 3 (CyHV-3) major capsid protein (MCP) and its impact on immune gene expression in vitro. سامانه مدیریت نشریات علمی, 24(5), 1223-1234. doi: 10.22092/ijfs.2025.134332
K.S. Li; Y.M. Zhu; X. Zhou; Y.P. Hu; C.Z. Ju; Y.T. Wang; M.J. Hu; C. Zhang; J.Q. Zhang. "Molecular characterization of cyprinid herpesvirus 3 (CyHV-3) major capsid protein (MCP) and its impact on immune gene expression in vitro". سامانه مدیریت نشریات علمی, 24, 5, 1404, 1223-1234. doi: 10.22092/ijfs.2025.134332
Li, K.S., Zhu, Y.M., Zhou, X., Hu, Y.P., Ju, C.Z., Wang, Y.T., Hu, M.J., Zhang, C., Zhang, J.Q.. (1404). 'Molecular characterization of cyprinid herpesvirus 3 (CyHV-3) major capsid protein (MCP) and its impact on immune gene expression in vitro', سامانه مدیریت نشریات علمی, 24(5), pp. 1223-1234. doi: 10.22092/ijfs.2025.134332
Li, K.S., Zhu, Y.M., Zhou, X., Hu, Y.P., Ju, C.Z., Wang, Y.T., Hu, M.J., Zhang, C., Zhang, J.Q.. Molecular characterization of cyprinid herpesvirus 3 (CyHV-3) major capsid protein (MCP) and its impact on immune gene expression in vitro. سامانه مدیریت نشریات علمی, 1404; 24(5): 1223-1234. doi: 10.22092/ijfs.2025.134332

Molecular characterization of cyprinid herpesvirus 3 (CyHV-3) major capsid protein (MCP) and its impact on immune gene expression in vitro

Iranian Journal of Fisheries Sciences
دوره 24، شماره 5، آذر 2025، صفحه 1223-1234    اصل مقاله (1.36 M)
شناسه دیجیتال (DOI): 10.22092/ijfs.2025.134332
نویسندگان
K.S. Li1؛ Y.M. Zhu1؛ X. Zhou2؛ Y.P. Hu1؛ C.Z. Ju1؛ Y.T. Wang1؛ M.J. Hu1؛ C. Zhang1؛ J.Q. Zhang* 1
1Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, China
2Technology Center of Wuhan Customs, Wuhan, China
چکیده
Main chitin protein (MCP) is a crucial component of cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV), found on the surface of the viral capsid. It plays a vital role in protecting viral DNA and assembling viral particles, contributing to the virus's high infectivity and lethality. This study aims to characterize the MCP-encoded protein of CyHV-3, investigate its subcellular localization, and assess its impact on the expression of immune factors through in vitro experiments. Our findings revealed that the CyHV-3 MCP protein consists of 579 amino acids. Despite analyzing evolutionary trees of homologous proteins from three carp herpesvirus sources, no significant similarities were observed. Subcellular localization experiments indicated that the green fluorescent signal of the pEGFP- MCP protein predominantly diffused within the cytoplasm. Remarkably, overexpression of MCP significantly suppressed the expression of immune factors in Channel Catfish Ovary (CCO) and Epithelioma papulosum cyprinid (EPC) cell lines, highlighting its role as a viral immune escape factor. These results enhance our understanding of the immune function of CyHV-3 MCP and provide a theoretical foundation for developing new vaccines against CyHV-3 infections. These results provide further insights into the immune function of CyHV-3 MCP and provide a theoretical basis for the development of new vaccines against CyHV-3 virus infection.
کلیدواژه‌ها
CyHV-3؛ MCP؛ Evolutionary trees؛ Subcellular localization؛ Immunity
آمار
تعداد مشاهده مقاله: 80
تعداد دریافت فایل اصل مقاله: 47


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب