| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,061 |
| تعداد مقالات | 34,172 |
| تعداد مشاهده مقاله | 46,752,546 |
| تعداد دریافت فایل اصل مقاله | 77,750,769 |
Prevalence of qnrA, qnrB, and qnrS genes in Klebsiella pneumoniae isolates from patients in hospitals in Sabzevar, Iran: A cross-sectional from February 2022 to October 2023 | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 22 مرداد 1404 اصل مقاله (536.29 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2025.368374.3523 | ||
| نویسندگان | ||
| Faezeh Kheirabadi1؛ Maryam Rostazadeh2؛ Ehsan Javaheri3؛ Tahereh Navidifar4؛ Mohammad Keyvanloo Shahrestanaki5؛ Saeed Khoshnood6؛ Mohsen Heidary* 7؛ Reza Faraji* 8 | ||
| 1Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran | ||
| 2Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Leishmaniosis Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran | ||
| 4Department of Basic Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran | ||
| 5Department of Nutrition and Biochemistry, School of Medicine, Sabzevar University of Medical Sciences | ||
| 6Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran | ||
| 7Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran | ||
| 8Tuberculosis and Lung Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran | ||
| چکیده | ||
| Klebsiella pneumoniae (K. pneumoniae) has emerged as a significant opportunistic pathogen responsible for nosocomial infections, including hospital-acquired pneumonia (HAP) and various intra-abdominal infections. The aim of this study was to investigate the prevalence of qnrA, qnrB, and qnrS genes in K. pneumoniae isolates from patients in hospitals in Sabzevar, Iran. Of 100 specimens of urine, respiratory secretions, blood, tracheal lavage, pleural fluid and trachea were collected from patients referred to hospitals in Sabzevar, northeastern Iran. Identification of bacteria was performed by gram stain, culture characteristics and biochemical methods. Bacterial susceptibility to quinolone antibiotics using the Kirby-Bauer disk diffusion method according to Clinical Laboratory Standards Institute (CLSI) guidelines (2023). The extracted DNA was subjected to polymerase chain reaction (PCR) assay targeting three genes of qnrA, qnrB and qnrS using specific primers. The data were analyzed using the chi-squared test and the Fisher's exact test with the IBM SPSS Statistics version 26 software. P values less than 0.05 were also considered statistically significant. 100 non-duplicate K. pneumoniae isolates were collected from various clinical specimens, including urine (62%), respiratory tract secretions (6%), trachea (18%), blood (10%), tracheal lavage (3%), and pleural fluid (1%). The highest and lowest resistance were related to ampicillin (92%) and nitrofurantoin (22%), respectively. The qnrA, qnrB and qnrS genes were present in 61%, 56% and 47% of the isolates, respectively. There is a statistically significant association between the presence of qnr genes and resistance to fluoroquinolones (p<0.002). Based on these results, qnrA-producing K. pneumoniae strains were isolated from patients. Thus, prescribing appropriate antibiotics and detection of qnr genes is required and it can be useful in tracking, treating and knowledge of K. pneumoniae infection prevalence rate. | ||
| کلیدواژه‌ها | ||
| Klebsiella pneumoniae؛ antimicrobial susceptibility؛ qnr genes | ||
|
آمار تعداد مشاهده مقاله: 81 تعداد دریافت فایل اصل مقاله: 44 |
||