| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,139 |
| تعداد مقالات | 34,918 |
| تعداد مشاهده مقاله | 50,383,126 |
| تعداد دریافت فایل اصل مقاله | 90,276,515 |
In vitro evaluation of bio control agents and botanicals against mulberry root rot pathogen Fusarium oxysporum | ||
| Journal of Organic Farming of Medicinal Plants | ||
| دوره 3، شماره 1، خرداد 2024، صفحه 44-49 اصل مقاله (469.51 K) | ||
| نوع مقاله: Original Article | ||
| نویسندگان | ||
| hadi Rahanandeh* 1؛ Ezatollah Sedaghatfar2؛ Mahsa Moshayedi3؛ nadiah Nazari4؛ Saeid Chavoshi5 | ||
| 1Plant Pathology Department, Faculty of Agriculture, Islamic Azad University, Rasht Branch | ||
| 2Department of Plant protection, Ar.C., islamic Azad University, Arak, Iran | ||
| 3Member of the Agricultural Engineering System of Guilan Province | ||
| 4Graduated in Plant Pathology, Faculty of Agriculture, Islamic Azad University, Rasht Branch | ||
| 5Department of Agronomy And Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran | ||
| چکیده | ||
| Mulberry is only source of food for the silkworm (Bombyx mori) L. Mulberry is sensitive to soil borne diseases among them root rot is fast spreading disease caused by F. oxysporum. Pathogenes can be managed by synthetic chemical fungicides but the use of synthetic fungicides results in Toxic resedues on the growth and development of silkworms. In this study, the effect of antagonistic biological agents for the management of mulberry root rot pathogen was investigated. The extract of five herbs is used: garlic, peppermint, tea, thyme, and neem. The extracts were tested at three concentrations of 10, 10-1, 10-3 and three solvents of ethanol, methanol and water. The effect of the extracts in a certain concentration was investigated based on the method of mixing with the culture medium. Bacteria were purified from soil using serial dilution method and were examined based on dual culture, volatile compounds, extracellular extract, and antibiotics. The highest inhibition rate was observed in extracts obtained from ethanol solvent. Ethanolic extract of all plants except peppermint showed 100% inhibition. The least effect was observed for extracts dissolved in water. The highest inhibition rate water solvent was observed in neem extract with 58.34. In different solvents, the lowest inhibition rate was observed in extracts obtained from peppermint. 100 bacterial isolates were isolated from soil samples, of which five isolates showed antagonistic ability in various tests. The top bacteria were identified based on biochemical and molecular factors and belonged to the two genera Pseudomonas and Bacillus. | ||
| کلیدواژهها | ||
| Plant extract؛ Mulberry root rot؛ Antagonist bacteria؛ Fusarium oxysporum | ||
| مراجع | ||
|
Alizadeh, M., Vasebi, Y. & Safaie, N. (2020). Microbial antagonists against plant pathogens in Iran: A review. Open Agriculture, 5(1), 404-440. DOI: 10.1515/opag-2020-0031
Almeida, F. A. C., et al. (2017). "Neem extract as a potential fungicide for controlling Fusarium wilt in tomato plants. Crop Protection, 92, 109-115. https://doi.org/10.1016/j.cropro.2016.10.012.
Bhalerao, T. S., Kelkar, T. S, & Dey, U. (2015). Plant extracts: A novel approach for controlling plant pathogens. International Journal of Current Microbiology and Applied Sciences, 4(1), 105-112.
Compant, S., Duffy, B., Nowak, J., Clément, C. & Barka, E. A. (2005). Use of plant growth-promoting bacteria for biocontrol of plant diseases: Principles, mechanisms of action, and future prospects. Applied and Environmental Microbiology, 71(9), 4951-4959.
Goel, N., Anukrati, K. & Paul, P.K. (2016). Anti-phytopathogenic and SAR inducing properties of Neem: A review. Journal of Chemical and Pharmaceutical Sciences, 9(4), pp.2547-2555.
Haas, D. & Défago, G. (2005). Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature Reviews Microbiology, 3(4), 307-319.
Ongena, M. & Jacques, P. (2008). Bacillus lipopeptides: Versatile weapons for plant disease biocontrol. Trends in Microbiology, 16(3), 115-125.
Kwak, M. J., et al. (2018). Rhizosphere microbiome structure alters to enable wilt resistance in tomato. Nature Biotechnology, 36(11), 1100-1109.
Köhl, J., Kolnaar, R. & Ravensberg, W. J. (2019). Biocontrol of plant diseases by microbial consortia: Mechanisms, applications, and perspectives. Frontiers in Microbiology, 10, 2846. DOI: 10.3389/fmicb.2019.02846
Kumar, S., Kumar, R. & Khan, A. (2018). Medicinal plants and their uses in agriculture: A review. Journal of Pharmacognosy and Phytochemistry, 7(1), 1815-1821.
Pandey, A. K., Singh, P. & Tripathi, N. N. (2016). Chemistry and bioactivities of essential oils of some Ocimum species: An overview. Asian Pacific Journal of Tropical Biomedicine, 4(9), 682-694.
Moshayedi, M., Rahanandeh, H. & Hamzeh, A. (2017). In vitro evaluation of some fungicides and tea extract against Pestalotia sp. and Colletotrichum sp., the causal agents of leaf spot and anthracnose of azalea. journal of ornamental and horticultural plants. 1: 45-51.
Qin, L., Qi, J., Shen, G., Qin, D., Wu, J., Song, Y., ... & Xia, Q. (2022). Effects of microbial transfer during food-gut-feces circulation on the health of Bombyx mori. Microbiology Spectrum, 10(6), e02357-22.
Rahanandeh, H., Moshayedi, M. & Hamzeh, A. (2017). Biological control of onion pink root using antagonistic strains of bacteria. Philippine Journal of Crop Science. 42 (3), 71-78.
Rahanandeh, H. & Sedaghat far, E. (2022). The inhibitory effect of five plant extracts on the pathogen of mulberry dieback disease (Sclorotinia sclerotiorum) in laboratory conditions. Applied Plant Protection.11(1), 29-34.
Rana, M., Sharma, A. & Sharma, S. (2017). Challenges in biological control of plant pathogens. Journal of Plant Diseases and Protection, 124(6), 529-538.
Raut, L.S., Dalvi, S.M. & Rakh, R.R. (2024). Antifungal Potential of Bacillus subtilis subsp. inaquosorum RLS76 for Management of Fusarium Wilt Disease of Bt-Cotton. Current Agriculture Research Journal, 12(1).
Schaad, N.W., Jones, J.B. & Chun, W. (2001). Laboratory guide for the identification of plant pathogenic bacteria (No. Ed. 3, pp. xii+-373).
Seetha, R.J., Raja, G.R.C. & Ramanjaneyulu, R. ( 2010). Evaluation of Certain Plant Extracts and Antagonists Against Fusarium solani and Alternaria tenuissima, the Incitants of Root Rot and Die-Back Diseases of Mulberry. International Journal of Industrial Entomology and Biomaterials, 20(1), pp.1-5.
Šegvić Klarić, M., Kosalec, I., Mastelić, J., Pieckova, E. & Pepeljnak, S. (2007). Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings. Letters in applied microbiology, 44(1), 36-42.
Singh, D., Sharma, R. R. & Khanna, K. (2020). Biological control of Fusarium wilt: A review. Journal of Plant Pathology, 102(2), 315-328.
Sultana, R. & Kim, K. (2015). Bacillus thuringiensis C25 suppresses popcorn disease caused by Ciboria shiraiana in mulberry (Morus australis L.). Biocontrol Science and Technology, 26(2), 145–162. https://doi.org/10.1080/09583157.2015.1084999
Rahman, K. (2007). Garlic and aging: New insights into an old remedy. Ageing Research Reviews, 6(1), 46-62.
Sundaramoorthy, S., Raghuchandr, T., Raghupathi, N. & Samiyappan, R. (2012). Combinatorial effect of endophytic and plant growth-promoting rhizobacteria against wilt disease of Capsicum annuum L. caused by Fusarium solani. Biological Control. 60(1), 59-67.
Wang, Q., Sun, Z., Ma, S., Liu, X., Xia, H. & Chen, K. (2022). Molecular mechanism and potential application of bacterial infection in the silkworm, Bombyx mori. Developmental & Comparative Immunology, 131, p.104381.
Yang, X., Bai, S., Wu, J., Fan, Y., Zou, Y., Xia, Z., Ao, J., Chen, T., Zhang, M. & Yang, R. (2023). Antifungal activity and potential action mechanism of Allicin against Trichosporon asahii. Microbiology Spectrum, 11(3), pp.e00907-23.
Yu, C., Hu, X., Deng, W., Li, Y., Han, G. & Ye, C. (2016). Soil fungal community comparison of different mulberry genotypes and the relationship with mulberry fruit sclerotiniosis. Scientific reports, 6(1), p.28365. | ||
|
آمار تعداد مشاهده مقاله: 139 تعداد دریافت فایل اصل مقاله: 107 |
||