| Nepeta pungens (Bunge) Benth. and N. racemosa Lam., both members of the Lamiaceae family, are valuable medicinal plants in Qazvin Province, Iran. This study investigated their meiotic behavior and pollen fertility due to their medicinal importance. Meiosis was studied in pollen mother cells using the squash technique, while pollen viability was assessed based on staining ability. Chromosomal analysis revealed that both species are diploid, with a chromosome number of 2n=2x=18. Both species exhibited meiotic abnormalities, including chromosomal stickiness, laggards, bridges, cytomixis, non-synchronous segregation, micronuclei, tripolar and pentapolar spindles, at varying frequencies. The meiotic index was 94% for N. racemosa and 86% for N. pungens. Pollen fertility tests showed that N. racemosa (89%) had a higher proportion of fertile pollen grains compared to N. pungens (83%). These findings enhance our understanding of the fundamental biological and genomic characteristics of these species, providing a basis for future research. Additionally, they highlight the potential implications of meiotic instability for plant breeding and conservation strategies, particularly in medicinally important species. |
- Rechinger, K. H. Nepeta in Flora Iranica, Labiatae. In: Rechinger KH (ed.) Akademische Druck-U. Verlagsanstalt Publication. 1982; 108-216.
- Jamzad Z. Nepeta L. in Flora of Iran, Lamiaceae In: Assadi et al. (eds.) Research Institute of Forests & Rangelands publication.2012;76: 454-609.
- Love A. IOPB chromosome number reports LVII. Taxon. 1977;26(4):443-52.
- Goldblatt P., Lowry Ii P. The index to plant chromosome numbers (Ipcn): Three decades of publication by the Missouri Botanical Garden come to An End. Annals of the Missouri Botanical Garden. 2011;98:226-27.
- Bordbar F., Mahani Z.E., Mirtadzadini M. New chromosome counts in Lamiaceae from the flora of Iran-I. Journal of Applied Biological Sciences. 2021;15(3):310-317.
- Hamant O., Ma H., Cande W. Genetics of meiotic prophase I in plants. Annual Review of Plant Biology. 2006;57:267-302.
- Kharazian N., Shourabi S.Z., Yousefi M. Chromosome count and karyotype study of eleven Nepeta L. (Lamiaceae) species from Iran. Biological Diversity and Conservation. 2013;6(1):76-87.
- Utsunomiya K.S., Bione N.C.P., Pagliarini M.S. How many different kinds of meiotic abnormalities could be found in a unique endogamous maize plant? Cytologia. 2002;67(2):69-76.
- Cai X., Xu S.S. Meiosis-driven genome variation in plants. Current Genomics. 2007;8(3):151-61.
- Chen Y.P., Zhao F., Peng H., Xiang C.L., Funamoto T. Chromosome numbers of 24 taxa of Lamiaceae from Southwest China. Caryologia. 2018;71(4):298-306.
- Hasaninejad M., Jamzad Z., Afsharzadeh S., Saeedi H. Cytological studies on four endemic species of Nepeta L.(Lamiaceae). The Iranian Journal of Botany. 2020;26(1):62-66.
- Hasaninejad M., Jamzad Z., Afsharzadeh S., Saeidi H. Chromosome counts of eight Iranian endemic species of Nepeta L. (Lamiaceae). Caryologia. 2021;74(1):53-61.
- Pagliarini M.S. Meiotic behaviour of economically important plant species: the relationship between fertility and male sterility. Genetics and Molecular Biology. 2000;23:997-1002.
- Defani-Scoarize M.A. Evaluation of meiotic behavior in double-cross maize hybrids and their parents. Maydica. 1995;40:319-24.
- Baptists-Giacomoelli F.R., Pagliarini M.S., Almeida J.L. Elimination of micronuclei from microspores in a Brazilian oat (Avena sativa L.) variety. Genetics and Molecular Biology. 2000;23:681-84.
- Tantray Y.R., Jan I., Wani M.S., Singhal V.K., Gupta R.C. Chromosome numbers and meiotic behavior in some species of Asteraceae from high altitudinal regions of Kashmir Himalayas. Journal of Asia-Pacific Biodiversity. 2021;14:590-606.
- Saggoo M.I.S., Srivastava D.K., Grewal P. Meiotic studies in 14 species of the Nepeta L. (Lamiaceae) from cold desert regions of Lahaul-Spiti and adjoining areas of Northwest-Himalaya, India. Cytologia. 2011;76(3):231-36.
- Kaur M., Singhal V.K. First report of cytomixis and meiotic abnormalities in Nepeta govaniana from Solang Valley, Kullu District, Himachal Pradesh. Cytologia. 2014;79(2):227-233.
- Malik R.A., Gupta R.C., Singh V., Bala S., Kumari S. New chromosome reports in Lamiaceae of Kashmir (northwest Himalaya), India. Protoplasma. 2017;254:971-85.
- Duda S.C., Marghitaş L.A., Dezmirean D., Duda M., Margaoan R., Bobiş O. Changes in major bioactive compounds with antioxidant activity of Agastache foeniculum, Lavandula angustifolia, Melissa officinalis and Nepeta cataria: Effect of harvest time and plant species. Industrial Crops and Products. 2015;77:499-507.
- Consolaro M.E.L., Pagliarini M.S. Spontaneous chromosome stickiness in microsporocytes of Centella asiatica (L.) Urban (Umbelliferae). Cytologia. 1996;61(1):57-61.
- Nicklas R.B., Ward S.C. Elements of error correction in mitosis: microtubule capture, release, and tension. The Journal of Cell Biology. 1994;126(5):1241-1253.
- Wani B.A., Magray J.A., Qadir R.U., Javid H., Ganie A.H., Nawchoo I.A. Meiotic bottlenecks compromises reproductive success by impairing pollen fertility and seed set in Swertia thomsonii C.B. Clarke—an important endemic medicinal herb of western Himalaya. Cytologia. 2022;87(4):323-30.
- Raina A.T.Y., Khan S. Assessment of bio-physiological damages and cytological aberrations in cowpea varieties treated with gamma rays and sodium azide. Plos One. 2023;18(7):e0288590.
- Jabeen S.Z.M., Ahmad M., Ali M.A., Elshikh M.S., Makhkamov T., Mamarakhimov O., Yuldashev A., Khaydarov K., Gafforov Y., Baysunov B., Mammadova A.O., Botirova L., Sultana S., Majeed S., Rozina A.S, Abid A., Rahmatov A. Micrometer insights into Nepeta genus: pollen micromorphology unveiled. Micron. 2024;177:103574.
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