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Comparative analysis of two consecutive genome sequencing results of Enterococcus faecium strain EntfacYE | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 10 خرداد 1404 اصل مقاله (347.17 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2025.367060.3339 | ||
| نویسندگان | ||
| Maryam Yazdanizad1؛ Ali Akbar Saboor Yaraghi2؛ Golshid Javdani Shahedin3؛ Mahla Asadian4؛ Ramin Mazaheri Nezhad Fard* 5 | ||
| 1Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran | ||
| 2Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Pasteur Institute, Tehran, Iran | ||
| 4Department of pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| 5Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| The overuse of antimicrobials in healthcare has driven emergence, persistence, and rapid spread of antimicrobial-resistant pathogens. Vancomycin-resistant enterococci, majorly Enterococcus faecium, have recently emerged as multidrug-resistant bacteria worldwide. Therefore, enterococcal infections are more challenging to treat due to their increased multiple-drug resistance. Studying genome of an enterococcal isolate and investigating genome changes over time help researchers better understand antimicrobial resistance development in bacterial isolates. In the present study, E. faecium EntfacYE isolate from a human biological sample was assessed. After phenotypic, biochemical and molecular verifications of the bacterial isolate, the bacterial genome was wholly sequenced. In total, the EntfacYE genomic subsystems contained 23 categories with 46 antimicrobial resistance genes. In a previous study by Elahi et al., 59 antimicrobial resistance genes were reported for this isolate. In the current study, 31 antimicrobial resistance genes were reported in the subsystems and 15 genes had no subsystems, while these categories were respectively reported as 49 and ten in the previous study. Genes of tetracycline resistance were reported in this study, unlike the previous study. Despite the short time interval between the two studies, increases in the number and type of antimicrobial resistance genes were recorded in the current study, indicating that bacteria are becoming rapidly resistant to the available antimicrobials. In general, study of antimicrobial resistance genes in bacteria can be effective in better understanding of the resistance patterns and mechanisms, which can lead to find novel protocols for limiting spread of antimicrobial resistance in bacteria. Keywords: Whole-genome sequencing, Enterococcus faecium, Biological samples, Antimicrobial resistance | ||
| کلیدواژه‌ها | ||
| Whole-genome sequencing؛ Enterococcus faecium؛ Biological samples؛ Antimicrobial resistance | ||
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آمار تعداد مشاهده مقاله: 252 تعداد دریافت فایل اصل مقاله: 66 |
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