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Mohamadzadeh, Tahereh, Arsalani, Saeideh, Hafezi, Fatemeh, Sadjjadi, Seyed Mahmoud, Heidari, Soudabeh, Sharifzadeh, Parviz, Darabi, Enayat. (1404). Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method. سامانه مدیریت نشریات علمی, 80(4), 1023-1028. doi: 10.32592/ARI.2025.80.4.1023
Tahereh Mohamadzadeh; Saeideh Arsalani; Fatemeh Hafezi; Seyed Mahmoud Sadjjadi; Soudabeh Heidari; Parviz Sharifzadeh; Enayat Darabi. "Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method". سامانه مدیریت نشریات علمی, 80, 4, 1404, 1023-1028. doi: 10.32592/ARI.2025.80.4.1023
Mohamadzadeh, Tahereh, Arsalani, Saeideh, Hafezi, Fatemeh, Sadjjadi, Seyed Mahmoud, Heidari, Soudabeh, Sharifzadeh, Parviz, Darabi, Enayat. (1404). 'Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method', سامانه مدیریت نشریات علمی, 80(4), pp. 1023-1028. doi: 10.32592/ARI.2025.80.4.1023
Mohamadzadeh, Tahereh, Arsalani, Saeideh, Hafezi, Fatemeh, Sadjjadi, Seyed Mahmoud, Heidari, Soudabeh, Sharifzadeh, Parviz, Darabi, Enayat. Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method. سامانه مدیریت نشریات علمی, 1404; 80(4): 1023-1028. doi: 10.32592/ARI.2025.80.4.1023

Comparison of the efficacy of in-house-produced AgB with a domestic commercial kit for the serodiagnosis of human cystic echinococcosis by the ELISA method

Archives of Razi Institute
مقاله 24، دوره 80، شماره 4، مهر و آبان 2025، صفحه 1023-1028    اصل مقاله (448.14 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.4.1023
نویسندگان
Tahereh Mohamadzadeh1، 2؛ Saeideh Arsalani3؛ Fatemeh Hafezi4؛ Seyed Mahmoud Sadjjadi5؛ Soudabeh Heidari3؛ Parviz Sharifzadeh6؛ Enayat Darabi* 7
1Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Health Research center, Life style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4Department of Parasitology and Mycology, School of Medicine, Semnan University of Medical Sciences, Semnan,
5Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
6Department of Pathology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
7Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Abstract
Cystic echinococcosis (CE) is a common zoonotic infection caused by the metacestode form of Echinococcus granulosus. The disease is found worldwide, and Iran is considered an endemic area. Depending on the affected organ, the disease has different clinical signs and symptoms. Humans are regarded as dead-end intermediate hosts. CE cysts commonly develop in the liver and lungs, while they are less frequently found in other organs. Timely diagnosis of this disease is essential in humans. Diagnosis relies on imaging methods (X-ray, ultrasound, MRI, and CT scan) complemented by serological testing. The present study used the indirect ELISA method to compare the efficacy of in-house-produced antigen B (AgB) and a domestic commercial kit. Hydatid cyst fluid (HCF) was extracted from the sheep liver infected with cystic echinococcosis cyst followed by preparation of AgB. It was used in the indirect ELISA test. A total of 142 sera consisting of 36 patients with pathologically confirmed CE, 46 cases with other parasitic diseases, and 60 healthy cases were examined using in-house AgB and a domestic commercial ELISA kit. Our findings revealed the sensitivity and specificity of the indirect ELISA using in-house AgB to be 86.11% and 95.28% respectively. The commercial kit demonstrated a sensitivity of 77.78% and a specificity of 99.06%. Although both in-house-produced AgB and commercial kit showed considerable and relatively similar diagnostic efficiency, the study results showed a higher sensitivity and validity of AgB compared with the commercial ELISA kit.
Keywords: Cystic echinococcosis, Diagnosis, AgB, ELISA
Keywords: Cystic echinococcosis, Diagnosis, AgB, ELISA
کلیدواژه‌ها
Cystic echinococcosis؛ Diagnosis؛ AgB؛ ELISA
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