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Comparison of the efficacy of in-house produced AgB with a domestic commercial kit for serodiagnosis of Human Cystic Echinococcosis by ELISA method | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 12 فروردین 1404 اصل مقاله (205.75 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2025.365501.3093 | ||
| نویسندگان | ||
| Tahereh Mohamadzadeh1؛ saeideh Arsalani2؛ Fatemeh Hafezi3؛ Seyed Mahmoud Sadjjadi4؛ Soudabeh Heidari2؛ Parviz Sharifzadeh5؛ Enayat Darabi* 6 | ||
| 1Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| 2Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences (BMSU), Tehran, Iran | ||
| 3Department of Parasitology and Mycology, School of Medicine, Semnan University of Medical Sciences, Semnan, | ||
| 4Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 5Department of Pathology, School of Medicine, Baqiyatallah University of Medical Sciences, (BMSU), Tehran, Iran | ||
| 6Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Abstract Cystic echinococcosis (CE) is a common zoonotic infection caused by the metacestode form of Echinococcus granulosus. The disease is found worldwide, and Iran is considered an endemic area. Depending on the affected organ, the disease has different clinical signs and symptoms. Humans are regarded as dead-end intermediate hosts. CE cysts commonly develop in the liver and lungs, while they are less frequently found in other organs. Timely diagnosis of this disease is essential in humans. Diagnosis relies on imaging methods (X-ray, ultrasound, MRI, and CT scan) complemented by serological testing. The present study used the indirect ELISA method to compare the efficacy of in-house-produced antigen B (AgB) and a domestic commercial kit. Hydatid cyst fluid (HCF) was extracted from the sheep liver infected with cystic echinococcosis cyst followed by preparation of AgB. It was used in the indirect ELISA test. A total of 142 sera consisting of 36 patients with pathologically confirmed CE, 46 cases with other parasitic diseases, and 60 healthy cases were examined using in-house AgB and a domestic commercial ELISA kit. Our findings revealed the sensitivity and specificity of the indirect ELISA using in-house AgB to be 86.11% and 95.28% respectively. The commercial kit demonstrated a sensitivity of 77.78% and a specificity of 99.06%. Although both in-house-produced AgB and commercial kit showed considerable and relatively similar diagnostic efficiency, the study results showed a higher sensitivity and validity of AgB compared with the commercial ELISA kit. Keywords: Cystic echinococcosis, Diagnosis, AgB, ELISA Keywords: Cystic echinococcosis, Diagnosis, AgB, ELISA | ||
| کلیدواژه‌ها | ||
| Cystic echinococcosis؛ Diagnosis؛ AgB؛ ELISA | ||
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