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Nwangwa, Justina, Umoh, Ekementeabasi, ENENE, ESU. (1404). Comparative study of Amatem and ethanol leaf extract of Mangifera indica on male reproductive hormones and histology of the testis. سامانه مدیریت نشریات علمی, 80(2), 435-446. doi: 10.32592/ARI.2025.80.2.435
Justina Nwandimma Nwangwa; Ekementeabasi Aniebo Umoh; ESU UKPAI ENENE. "Comparative study of Amatem and ethanol leaf extract of Mangifera indica on male reproductive hormones and histology of the testis". سامانه مدیریت نشریات علمی, 80, 2, 1404, 435-446. doi: 10.32592/ARI.2025.80.2.435
Nwangwa, Justina, Umoh, Ekementeabasi, ENENE, ESU. (1404). 'Comparative study of Amatem and ethanol leaf extract of Mangifera indica on male reproductive hormones and histology of the testis', سامانه مدیریت نشریات علمی, 80(2), pp. 435-446. doi: 10.32592/ARI.2025.80.2.435
Nwangwa, Justina, Umoh, Ekementeabasi, ENENE, ESU. Comparative study of Amatem and ethanol leaf extract of Mangifera indica on male reproductive hormones and histology of the testis. سامانه مدیریت نشریات علمی, 1404; 80(2): 435-446. doi: 10.32592/ARI.2025.80.2.435

Comparative study of Amatem and ethanol leaf extract of Mangifera indica on male reproductive hormones and histology of the testis

Archives of Razi Institute
مقاله 7، دوره 80، شماره 2، خرداد و تیر 2025، صفحه 435-446    اصل مقاله (528.45 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.2.435
نویسندگان
Justina Nwandimma Nwangwa1؛ Ekementeabasi Aniebo Umoh* 2؛ ESU UKPAI ENENE3
1Department of Physiology, University of Calabar, Calabar, Cross River State, Nigeria.
2Department of Physiology, Arthur Jarvis University, Akpabuyo, Cross River State, Nigeria
3Department of Physiology, Federal University Wukari, Wukari, Taraba State, Nigeria.
چکیده
Conflicting reports on reproductive disorders following the use of antimalarials have been recorded. This research aimed at comparing an orthodox drug (Amatem) with a herbal remedy (Mangifera indica) used for treating malaria on male reproductive hormone with the view of ascertaining which will be protective on reproductive parameters. Twenty-five male rats weighing 250-300 g were used for this study. Animals were divided into five groups, namely: Control, low dose M. indica (Mag [LD]), middle dose M. inidca (Mag [MD]), high dose M indica (Mag [HD]), and Amatem group. All animals were allowed access to normal rat chow and water throughout the period of the experiment. Following the oral toxicity study, Mag [LD], Mag [MD], and Mag [HD] were administered ethanolic extract of M. indica leaf at 400 mg/kg, 800 mg/kg, and 1600 mg/kg body weight. Amatem was dissolved in normal saline and administered following the recommended dose of 8 mg/kg body weight. All administration lasted for one week, and sacrifice was carried out on the eighth day. Blood was gotten via cardiac puncture and spun in a centrifuge to get the serum, which was used for testosterone, FSH, and LH assays through the method of enzyme-linked immunoassay (ELISA). The testis was excised and histology done using H&E staining. ANOVA was used to test the level of significance of results among experimental groups. Serum testosterone was higher in Amatem, Mag (MD), and Mag (HD) compared to the control at p < 0.05. There was also a significant increase in testosterone of Amatem compared to Mag (MD) and Mag (HD) at p < 0.05. Follicle stimulating hormone (FSH) and luteinizing hormone (LH) of Amatem, Mag [LD], Mag [MD], and Mag [HD] showed no significant difference with that of the control group. Mild to severe distortions were seen in the histology of the testis in the extract and drug-administered groups. Though Amatem and M. indica increase testosterone levels, caution is needed, as the distortions of the testis may pose harmful effects in the long run. Gas chromatography mass spectrometry (GC-MS) technique revealed 43 compounds, with heptadecane having the most peak areas of 8.00%. 
کلیدواژه‌ها
Malaria؛ Mango leaf extract؛ Antimalarial
مراجع
  1. Longley RJ, Malinga J, Hesping E, Sutanto E, DonVito SM, Kucharski M, Oyong DA, Verhoef JM, Du E, Villasis E, Mwikali K. MAM 2024: Malaria in a changing world. Trends in Parasitology. 2024;40(5):355-361.
  2. Villavicencio F, Perin J, Eilerts-Spinelli H, Yeung D, Prieto-Merino D, Hug L, et al. Global, regional, and national causes of death in children and adolescents younger than 20 years: an open data portal with estimates for 2000–21. The Lancet Global Health. 2024;12(1):e16-7.
  3. Summary of product characteristics (SmPC): Amatem soft gel. Retrieved from https://www.nafdac.gov.ng/wp-content/uploads/Files/SMPC/a_v5/SmPC-Amatem-20-120.pdf. 2024 Dec.
  4. Umoh EA, Okoroiwu HU, Uchenwa MO, Otu EEO, Asuquo JO, Osim, EE. Comparative effect of ginger (an anti-inflammatory medicinal herb) and aspirin (a non-steroidal anti-inflammatory drug) on cytoprotection and body weight changes in male albino Wistar rats. Journal of Biological Research-Bollettino della Società Italiana di Biologia Sperimentale. 2023;96(1):10633.
  5. Umoh EA, Uno VK, Effiong AE, Otu EE, Asuquo JO, Osim EE, et al. Comparative effect of ginger (an anti-inflammatory medicinal herb) and aspirin (a non-steroidal anti-inflammatory drug) on liver enzymes in male albino wistar rats. Journal of African Association of Physiological Sciences. 2023;11(1):29-35.
  6. Shah KA, Patel MB, Patel RJ, Parmar PK. Mangifera indica (mango). Pharmacognosy reviews. 2010;4(7):42.
  7. Malann YD, Matur BM, Mailafia S. Evaluation of the antimalarial activity of aqueous leaf extracts of Casuarina equistifolia and Mangifera indica against Plasmodium berghei in mice. Journal of Pharmacy & Bioresources. 2013;10(1):8-16.
  8. Asanga EE, Okoroiwu HU, Umoh EA, Eseyin OA, Oko MO, Okey FE, Oli M. Antiplasmodial Profiling of Mangifera indica's Herbal Formulation and Its Ability to Ameliorate Hematological Derangements in Plasmodium berghei-Infected Mice. Natural Product Communications. 2023;18(12):1-10.
  9. Akilah A, Balaha M, Abd-El Rahman MN, Hedya S. Apigenin and Baicalin, Each Alone or in Low Dose Combination, Attenuated Chloroquine Induced Male Infertility in Adult Rats. Thai Journal of Pharmaceutical Sciences (TJPS). 2018;42(3):118-128.
  10. Olumide SA, Raji Y. Long-term administration of artesunate induces reproductive toxicity in male rats. Journal of Reproduction & Infertility. 2011;12(4):249.
  11. Ejebe DE, Ojieh AE, Ovuakporaye SI, Odion-Obomhense HK, Adegor EC, Amadi CN, et al. Effects of anti-malarial alkaloids on the sperm properties and blood levels of reproductive hormones of adult men. African Journal of Biotechnology. 2008;7(19):3395-3400.
  12. Cole-Parmer Instrument Company. Solvent Extraction Method of Plants Using Ethanol. Retrived from https://www.coleparmer.com/blog/botanical-extraction-ethanol-method/. 2025 Jan.
  13. Reddeman RA, Glávits R, Endres JR, Clewell AE, Hirka G, Vértesi A, et al. A toxicological evaluation of mango leaf extract (Mangifera indica) containing 60% mangiferin. Journal of Toxicology. 2019;2019(3):1-14.
  14. Buss AD, Butler MS, et al. Natural product chemistry for drug discovery. Royal Society of Chemistry; 2010.
  15. Kelly DA, Nelson R. Characterization and quantification by gas chromatography of flavonoids. Journal of the Brazilian Chemical Society. 2014;25(2):109-115.
  16. Uno VK, Nwangwa JN, Umoh EA, Raymond AE, Asuquo JO, Otu EE, Beniangba MI. Morphology and Cyto-Architecture of the Testis and Assessment of Selected Biochemical Parameters Associated with Male Fertility Following the Consumption of Tom Brown Weaning Diet in Albino Wistar Rats. Natural Product Communications. 2024 Mar;19(3):1934578X241237923.
  17. SHEREEN MS, Mahmoud A, MOHAMED ZB, MOHAMED A. Effect of D-limonene on the age-related androgenic changes in male rats. The Medical Journal of Cairo University. 2020;88(2):599-609.
  18. Phuong NT, Ha MT, Nguyen DX, Nguyen NY, Huynh HA, Hau TP, et al. Development and antioxidant evaluation of mango leaf (Mangifera indica L.) extract loaded silk fibroin nanoparticles. Frontiers in Materials. 2024 Jul 17;11:1419697.
  19. Mofio BM, John R, Attah OR, Adikpe AO, Usende IL. Chronic oral exposure to Artemether–lumenfantrine induced testicular and epidydimal damage, germ cell death and severe decrease sperm viability in BALB/c mice. Scientific African. 2020;8(1):e00454.
  20. Umoh, E. A. Spermatogenesis. Retrieved from https://www.intechopen.com/online-first/1193826. 2024 Dec.
  21. Onyije FM. Drug: a major cause of infertility in male. Asian J Med Pharm Res. 2012;2(2):30-7.
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