This study aims to evaluate the antimicrobial activity of Ocimum basilicum L. leaf extracts due to the increasing prevalence of drug-resistant bacteria. The objective is to explore standardized analytical methods for isolating novel bioactive compounds from medicinal plants, which may provide innovative solutions for combating harmful microorganisms. The study involved the collection and preparation of O. basilicum leaves from Nasiriyah, Iraq. The leaves were cleaned, dried, and ground into powder. Twenty grams of the dried powder were mixed with 200 mL of ethanol and aqueous solvents, respectively, and subjected to ultrasonic extraction. The extracts were filtered, concentrated, and stored in sterile conditions. Antimicrobial activity was assessed using varying concentrations (25, 50, 75, and 100 mg/mL) against bacterial strains Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterobacter cloacae using the Kirby-Bauer disk diffusion method. The ethanolic extract exhibited the highest inhibitory effect against S. aureus, with a zone of inhibition measuring 15.6 ± 1.52 mm at 100 mg/mL. The aqueous extract showed significant activity against P. aeruginosa, with an inhibition zone of 12.1 ± 0.28 mm at 75 mg/mL. Comparative analysis revealed that both extracts outperformed several tested antibiotics in terms of efficacy against the respective bacterial strains. The findings indicate that O. basilicum extracts possess significant antimicrobial properties, making them potential alternatives to conventional antibiotics in treating bacterial infections. The study highlights the importance of these extracts in developing herbal formulations for antimicrobial applications, contributing to the field of alternative medicine. |
- Askary R.A., Malih H.R. Effectiveness study of Artemisia herba-alba and Borage officinalis leaf extract against bacteria Staphylococcus aureus. Uni Thi-Qar J. Sci. 2021;78:113-117.
- Ameen I.A., Okab H.F. Phyto-activity of Syzygium aromaticum extract against pathogenic bacteria isolated from chronic tonsilitis patients. Romanian J. Infect. Dis/Revista Romana de de Boli Infectioase 2024;127.
- Mulyaningsih S., Sporer F., Zimmermann S., Reichling J., Wink M. Synergistic properties of the terpenoids aromadendrene and 1, 8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine 2010;17:1061-1066.
- Septina E., Yetti R.D., Rivai H. Overview of Traditional Use, Phytochemical, and Pharmacological Activities of Chinese Petai (Leucaena leucocephala). Int. J. Pharm. Sci. Med. 2020;5:1-0.
- Wang S., Liu X.Q., Kang O.H., Kwon D.Y. Combination of sanguisorbigenin and conventional antibiotic therapy for Methicillin-resistant Staphylococcus aureus: Inhibition of biofilm formation and alteration of cell membrane permeability. International J. Mol. Sci. 2022;23:4232.
- Vaou N., Stavropoulou E., Voidarou C., Tsigalou C., Bezirtzoglou E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms 2021;9:2041.
- Dubale S., Kebebe D., Zeynudin A., Abdissa N., Suleman S. Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. J. Exp. Pharmacol. 2023;31:51-62.
- Nurzyńska-Wierdak R. Analiza zawartości i składu chemicznego olejku dwóch form bazylii wonnej (Ocimum basilicum L.). Annal. UMCS Sect. EEE. 2001;9:189-193.
- Kumar M., Rai D., Kanth N. Seasonal incidence, damage intensity and management of Ocimum leaf folder, Orphanostigma abruptalis on sweet basil (Ocimum basilicum). J. Entomolog. Res. 2022;:145-148.
- Calderón Bravo H., Vera Céspedes N., Zura-Bravo L., Muñoz L.A. Basil seeds as a novel food, source of nutrients and functional ingredients with beneficial properties: A review. Foods 2021;10:1467.
- Nadeem H.R., Akhtar S., Ismail T., Qamar M., Sestili P., Saeed W., Azeem M., Esatbeyoglu T. Antioxidant effect of Ocimum basilicum essential oil and its effect on cooking qualities of supplemented chicken nuggets. Antioxidants 2022;11:1882.
- Volpato G.T., Damasceno D.C., Calderon I.M., Rudge M.V. Review of Brazilian plants with proven hypoglycemic effect in the control of diabetes mellitus. Revista Brasileira de Plantas Medicinais 4:35-45
- Nguefack J., Budde B.B., Jakobsen M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett. Appl. Microbiol. 2004;39:395-400.
- Nair V.D., Cheruth A.J., Gopi R., Gomathinayagam M., Panneerselvam R. Antioxidant potential of Ocimum sanctum under growth regulator treatments. EurAsia J. BioSci. 2009;3:1-9.
- Nebedum J., Ajeigbe K., Nwobodo E., Uba C., Adesanya O., Fadare O., Ofusori D. Comparative study of the ethanolic extracts of four Nigerian plants against some pathogenic microorganisms. Res. J. Med. Plants 2009;3:23-28.
- Sánchez E., García S., Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholerae. Appl. Environ. Microbiol. 2010;76:6888-6894.
- Hemalatha R., Babu K.N., Karthik M., Ramesh R., Kumar B.D., Kumar P.U. Immunomodulatory activity and Th1/Th2 cytokine response of Ocimum sanctum in myelosuppressed swiss albino mice. Trends Med. Res. 2011;6:23-31.
- Prasad M.V., Khanum F. Antifatigue activity of ethanolic extract of Ocimum sanctum in rats. Res J. Med. Plants 2012;6:37-46.
- Tognolini M., Barocelli E., Ballabeni V., Bruni R., Bianchi A., Chiavarini M., Impicciatore M. Comparative screening of plant essential oils: phenylpropanoid moiety as basic core for antiplatelet activity. Life Sci. 2006;78:1419-1432.
- Jelačić S., Beatović D., Prodanović S., Tasić S.R., Moravčević D., Vujošević A., Vučković S. Chemical composition of the essential oil of basil (Ocimum basilicum L. Lamiaceae). Hemijska Industrija 2011;65:465-471.
- Ali B., Al-Wabel N.A., Shams S., Ahamad A., Khan S.A., Anwar F. Essential oils used in aromatherapy: A systemic review. Asian Pac. J. Trop. Biomed. 2015;5:601-611.
- Mostafavi S., Asadi-Gharneh H.A., Miransari M. The phytochemical variability of fatty acids in basil seeds (Ocimum basilicum L.) affected by genotype and geographical differences. Food Chem. 2019; 276:700-706.
- AlSaidy H.A., Alhasan D.A., Ali A.A. Reviews on plants gum and mucilage I: The composition and pharmacological significance of turmeric (Curcuma longa) rhizomes polysaccharides and mucilage/their aqueous extracts commercial products. Univ. Thi-Qar J. Sci. 2021;8:14-25.
- Hanan Z.K., Saleh M.B., Mezal E.H. Antimicrobial resistance pattern and plasmid profile of Salmonella enterica isolated from diarrheal children in Thi-Qar Province/Iraq. Univ. Thi-Qar J. Sci. 2020;7(2):49-53.
- Hussein N.N., Malih H.R. Inhibitory Effect of Tamarix aucheriana aqueous and alcoholic extracts against Klebsiella pneumonia which isolated from patients in Nasiriya City, Southern Iraq. Annals Roman. Soc. Cell Biol. 2021;25(6):12220-5.
- Chen F., Sun Y., Zhao G., Liao X., Hu X., Wu J., Wang Z. Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography–mass spectrometry. Ultrasonics Sonochem. 2007;14:767-778.
- Oladipo E.K., Awoyelu E.H., Adeosun I.J., Ayandele A.A. Antibacterial susceptibility of clinical isolates of Klebsiella pneumoniae in Nigeria to carbapenems. Iraqi J. Sci. 2021;396- 401.
- Lowenthal M.S., Andriamaharavo N.R., Stein S.E., Phinney K.W. Characterizing vaccinium berry standard reference materials by GC‐MS using NIST spectral libraries. Anal. Bioanal. Chem. 2013;405:4467-4476.
- World Health Organization. WHO model list of essential medicines, 20th list (March 2017, amended August 2017).
- Jain C., Khatana S., Vijayvergia R. Bioactivity of secondary metabolites of various plants: a review. Int. J. Pharm. Sci. Res. 2019;10:494-504.
- Farzaneh V., Carvalho I.S. A review of the health benefit potentials of herbal plant infusions and their mechanism of actions. Ind. Crop Prod. 2015;65:247-258.
- Kumar V., Sharma Y. Effects of environment on the chemical constituents and biological characteristics of some medicinal plants. Phytochemistry 2018;279-292.
- Kumar D., Karthik M., Rajakumar R. GC-MS analysis of bioactive compounds from ethanolic leaves extract of Eichhornia crassipes (Mart) Solms. and their pharmacological activities. Pharma. Innov. J. 2018;7:459-462.
- Kadhim M.J., Sosa A.A., Hameed I.H. Evaluation of anti-bacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier transform infrared (FT-IR) and gas chromatography-mass spectrometry (GC-MS) techniques. J. Pharmacogn. Phytother. 2016;8:127-146.
- Sivakumar C., Jeganathan K. Phytochemical profiling of cat whisker’s (Orthosiphon stamineus) tea leaves extract. J. Pharmacogn. Phytochem. 2018;7:1396-1402.
- Dandekar R., Fegade B., Bhaskar V.H. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. J. Pharmacogn. Phytochem. 2015;4:148-154
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