| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,871 |
| تعداد مقالات | 32,535 |
| تعداد مشاهده مقاله | 42,411,888 |
| تعداد دریافت فایل اصل مقاله | 19,187,959 |
Morphological and molecular characterization of a novel Neopestalotiopsis clavispora, causing rose stem canker in Iran | ||
| Mycologia Iranica | ||
| دوره 11، شماره 1، شهریور 2024، صفحه 101-109 اصل مقاله (835.19 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2024.367808.1294 | ||
| نویسندگان | ||
| Bahram Sharifnabi* 1؛ Mahdi Nourbakhsh2 | ||
| 1Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| 2Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| چکیده | ||
| Rose (Rosa spp.) is a widely cultivated perennial flowering plant grown in open fields and under controlled greenhouse conditions. This study focused on the isolation and characterization of a pathogen affecting Rosa hybrida plants in the Najafabad and Lenjan counties of Isfahan Province, Iran. Infected stems and crowns were collected from the greenhouses between July 2022 and September 2023. The cultivars affected were Samurai and Tanja. Fungal isolation was achieved, followed by morphological characterization and molecular identification by DNA sequencing of the internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1-α), and β-tubulin (TUB). Pathogenicity tests confirmed the ability of the isolate to induce stem canker and leaf spot symptoms in healthy Samurai and Tanja cultivars of rose (R. hybrida) plants, thereby fulfilling Koch's postulates. The isolates were identified as Neopestalotiopsis clavispora by the morphological and molecular characteristics. To our knowledge, this study reports the first identification of N. clavispora as a pathogen causing canker disease and dieback on roses in Iran, emphasizing the need for effective management strategies to protect rose health and mitigate economic losses in the ornamental horticultural sector. | ||
| کلیدواژه‌ها | ||
| Plant disease؛ Phylogenetic analysis؛ Morphological identification؛ Rose stem canker؛ Pathogenicity | ||
| مراجع | ||
|
Amirmijani, A., Fotouhifar, K., Atashi Khalilabd, A., and Seyedi, N. 2024. Pestalotiopsis biciliata, a new record for funga of Iran. Mycologia Iranica 11(1): 67-74.
Atashi Khalilabad, A., and Fotouhifar, K. B. 2022. Morphological and molecular characterization of a novel Pestalotiopsis trachycarpicola, causing Garden Croton leaf spot in Iran. Mycologia Iranica 9(1): 59-66.
Ayoubi, N. and Soleimani, M. J. 2016a. Morphological and molecular identification of Neopestalotiopsis asiatica causing leaf spot on sweet almond. Journal of Plant Pathology 98(2): 321–325.
Ayoubi, N. and Soleimani, M. J. 2016b. Strawberry fruit rot caused by Neopestalotiopsis iranensis sp. nov., and N. mesopotamica. Current Microbiology 72: 329-336.
Bhunjun, C. S., Phillips, A. J., Jayawardena, R. S., Promputtha, I. and Hyde, K. D. 2021. Importance of molecular data to identify fungal plant pathogens and guidelines for pathogenicity testing based on Koch’s Postulates. Pathogens 10(9):1096.
Borrero, C., Castaño, R. and Avilés, M. 2018. First report of Pestalotiopsis clavispora (Neopestalotiopsis clavispora) causing canker and twig dieback on blueberry bushes in Spain. Plant Disease 102(6): 1178-1178.
Brown, W. 1924. A method of isolating single strains of fungi by cutting out a hyphal tip. Annals of Botany 38(150): 402-404.
Caio, P., Bruno, F., Carlos, A. P. and Robert, B. 2021. Diaporthe rosiphthora sp. nov.: Yet another rose dieback fungus. Crop Protection 139: 105365.
Carbone, I. and Kohn, L. M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91(3): 553-556.
Daengsuwan, W., Wonglom, P. and Sunpapao, A. 2020. First report of Lasiodiplodia theobromae causing spadix rot in Anthurium andraeanum. Journal of Phytopathology 168(2): 129-133.
Daengsuwan, W., Wonglom, P., Arikit, S. and Sunpapao, A. 2021. Morphological and molecular identification of Neopestalotiopsis clavispora causing flower blight on Anthurium andraeanum in Thailand. Horticultural Plant Journal 7(6): 573-578.
Feng, Y. R., Liu, B. S. and Sun, B. B. 2014. First report of leaf blotch caused by Pestalotiopsis clavispora on Rosa chinensis in China. Plant Disease 98(7): 1009-1009.
Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61(4): 1323-1330.
Guba, E.F. 1961. Monograph of Pestalotia and Monochaetia. Harvard University Press, Cambridge. MA. USA.
Jia, J. Y., Li, X. H., Zhang, W., Zhou, Y. Y. and Yan, J. Y. 2019. First report of Botryosphaeria dothidea associated with stem canker on Rosa chinensis in China. Plant Disease 103(12): 3280.
Jiang, N., Bonthond, G., Fan, X. L. and Tian, C. M. 2018. Neopestalotiopsis rosicola sp. nov. causing stem canker of Rosa chinensis in China. Mycotaxon 133(2): 271-283.
Keith, L. M., Velasquez, M. E. and Zee, F. T. 2006. Identification and characterization of Pestalotiopsis spp. causing scab disease of guava, Psidium guajava L. in Hawaii. Plant Disease 90: 16-23.
Ketabchi, M. 2014. First report of banana fruit rot by Pestalotiopsis theae. Iranian Journal of Plant Pathology 50(1):103–104.
Liu, F., Hou, L., Raza, M. and Cai, L. 2017. Pestalotiopsis and allied genera from Camellia, with description of 11 new species from China. Scientific Reports 7(1): 866.
Maharachchikumbura, S. S., Hyde, K. D., Groenewald, J. Z., Xu, J. and Crous, P. W. 2014. Pestalotiopsis revisited. Studies in Mycology 79(1): 121-186.
Mirabolfathi, M. and Ershad, D. 2004. Twig and cane canker of rose in the greenhouses of central area of Iran. Iranian Journal of Plant Pathology 40: 84.
Mirtalebi, M., Banihashemi, Z., Sabahi, F. and Mafakheri, H. 2016. Dieback of rose caused by Acremonium sclerotigenum as a new causal agent of rose dieback in Iran. Spanish Journal of Agricultural Research 14(4): 24.
Murray, M. and Thompson, W. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321-4326.
Naeimi, S., Javadi, L. and Javadi, A. R. 2015. First report of Pestalotia disseminata, the causal agent of feijoa fruit rot in Iran. Mycologia Iranica 2(1):75-76.
Obregón, V. G., Meneguzzi, N. G., Ibañez, J. M., Lattar, T. E. and Kirschbaum, D. S. 2018. First report of Neopestalotiopsis clavispora causing root and crown rot on strawberry plants in Argentina. Plant Disease 102(9): 1856.
O’Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences 95(5): 2044-2049.
O'Donnell, K. and Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are non-orthologous. Molecular Phylogenetics and Evolution 7(1): 103-116.
Peng, C., Crous, P. W., Jiang, N., Fan, X. L., Liang, Y. M. and Tian, C. M. 2022. Diversity of Sporocadaceae (Pestalotioid fungi) from Rosa in China. Persoonia-Molecular Phylogeny and Evolution of Fungi 49(1): 201-260.
Pornsuriya, C., Chairin, T., Thaochan, N. and Sunpapao, A. 2020. Identification and characterization of Neopestalotiopsis fungi associated with a novel leaf fall disease of rubber trees (Hevea brasiliensis) in Thailand. Journal of Phytopathology 168(7-8): 416-427.
Qiu, F., Xu, G., Zheng, F. Q., Zhou, J., Zheng, L., Miao, W. G. and Xie, C. P. 2020. First report of Neopestalotiopsis clavispora causing leaf spot on macadamia (Macadamia integrifolia) in China. Plant Disease 104(1): 288.
Reddy, M. S., Murali, T. S., Suryanarayanan, T. S., Rajulu, M. G. and Thirunavukkarasu, N. 2016. Pestalotiopsis species occur as generalist endophytes in trees of Western Ghats forests of southern India. Fungal Ecology 24: 70-75.
Santos, C. C., Domingues, J. L., Santos, R. F. D., Spósito, M. B., Santos, A. and Novaes, Q. S. 2019. First report of Neopestalotiopsis clavispora causing leaf spot on Macadamia in Brazil. Plant Disease 103(7): 1790-1790.
Shi, J., Li, B., Wang, S., Zhang, W., Shang, M., Wang, Y. and Liu, B. 2024. Occurrence of Neopestalotiopsis clavispora causing Apple leaf spot in China. Agronomy 14(8): 1658.
Steyaert, R. L. 1949. Contribution à l'étude monographique de Pestalotia de Not. et Monochaetia Sacc. (Truncatella gen. nov. et Pestalotiopsis gen. nov.). Bulletin Jardin Botanique Etat Bruxelles 19: 285–354.
Sweets, L. E., Pfleger, F. L., Morgan, F. C. and Mizicko, J. R. 1982. Control of fungi associated with cankers of greenhouse roses. Plant Disease 66: 491-494.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30(12): 2725-2729.
Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22(22): 4673-4680.
Weir, B. S., Johnston, P. R. and Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Studies in mycology 73: 115-180.
White, T. J., Bruns, T., Lee, S. J. W. T. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315-322.
Widrlechner, M. P. 1981. History and utilization of Rosa damascena. Economic Botany 35: 42-58.
Wright, E. R., Pizzingrilli, P., Caligaris, M. V. and Cabral, D. 2007. Rose dieback caused by Trichothecium roseum in Argentina. Plant Disease 91(5): 631-631.
Zaher, E., Attia, M. and Reyad, N. E. H. 2012. Rose stem canker caused by Coniothyrium fuckelii Sacc. in Egypt. Egyptian Journal of Phytopathology 40(2): 39-55. | ||
|
آمار تعداد مشاهده مقاله: 107 تعداد دریافت فایل اصل مقاله: 200 |
||