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Eslamnezhad, Nima, Ghandehari, fereshte, Mirzaee, Mohsen, Mehrabi, Mohammad Reza, Madani, Mahboobeh. (1403). Molecular investigation and virulence determination of methicillin and vancomycin resistant clinical Staphylococcus aureus isolates. سامانه مدیریت نشریات علمی, 80(1), 209-216. doi: 10.32592/ARI.2025.80.1.209
Nima Eslamnezhad; fereshte Ghandehari; Mohsen Mirzaee; Mohammad Reza Mehrabi; Mahboobeh Madani. "Molecular investigation and virulence determination of methicillin and vancomycin resistant clinical Staphylococcus aureus isolates". سامانه مدیریت نشریات علمی, 80, 1, 1403, 209-216. doi: 10.32592/ARI.2025.80.1.209
Eslamnezhad, Nima, Ghandehari, fereshte, Mirzaee, Mohsen, Mehrabi, Mohammad Reza, Madani, Mahboobeh. (1403). 'Molecular investigation and virulence determination of methicillin and vancomycin resistant clinical Staphylococcus aureus isolates', سامانه مدیریت نشریات علمی, 80(1), pp. 209-216. doi: 10.32592/ARI.2025.80.1.209
Eslamnezhad, Nima, Ghandehari, fereshte, Mirzaee, Mohsen, Mehrabi, Mohammad Reza, Madani, Mahboobeh. Molecular investigation and virulence determination of methicillin and vancomycin resistant clinical Staphylococcus aureus isolates. سامانه مدیریت نشریات علمی, 1403; 80(1): 209-216. doi: 10.32592/ARI.2025.80.1.209

Molecular investigation and virulence determination of methicillin and vancomycin resistant clinical Staphylococcus aureus isolates

Archives of Razi Institute
مقاله 25، دوره 80، شماره 1، فروردین و اردیبهشت 2025، صفحه 209-216    اصل مقاله (432.53 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.1.209
نویسندگان
Nima Eslamnezhad1؛ fereshte Ghandehari* 1؛ Mohsen Mirzaee2؛ Mohammad Reza Mehrabi2؛ Mahboobeh Madani1
1Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
چکیده
Staphylococcus aureus is an opportunistic pathogen that provides conditions for host invasion due to various virulence factors and plays a role in causing various infections. These bacteria may have different pathogenic functions depending on the susceptibility of the host. This study investigates the sensitivity of S. aureus strains isolated from clinical samples to methicillin and vancomycin and evaluates the presence of resistance, virulence and toxin-producing genes and their expression level in the methicillin-restsnat S. aureus (MRSA), vancomycin-resistant S. aureus (VRSA), and vancomycin-intermediate S. aureus (VISA) isolates.
In a cross sectional study, 502 S. aureus isolates were collected from different infections during one year. Methicillin and vancomycin sensitivity of the isolates was checked by disk diffusion and microdilution broth method, respectively. The presence of resistance, adhesion, and toxin-producing genes was checked using the Multiplex PCR method. The expression level of the virulence and resistance genes was detected in resistant and sensitive isolates using real-time qPCR.
Out of 502 S. aureus isolates, 168 isolates (33.6%) were MRSA. A total of 6 isolates (1.2%) were diagnosed as VRSA and two isolates (0.4%) as VISA. Virulence and resistance-related genes showed different frequencies. The results of the gene expression study showed that the expression of most of the studied genes was significantly higher in resistant isolates (MRSA and VRSA) than in sensitive isolates.
VRSA and MRSA are considered severe threats to human health. The present study showed that sanitary measures are necessary to control this hospital pathogen more seriously due to the presence and expression of genes encoding virulence factors in S. aureus isolates.
کلیدواژه‌ها
Staphylococcus aureus؛ Vancomycin؛ Virulence genes؛ Toxin-producing؛ adhesion
مراجع
  1. Hemeg H, Ozbak H, Afrin F. Staphylococcus aureus. Intechopen, London. 2019.
  2. Guo Y, Song G, Sun M, Wang J, Wang Y Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus. Front Cell Infect Microbiol. 2020; 107(10):1-11.
  3. Harkins CP, Pichon B, Doumith M, Parkhill J, Westh H, Tomasz A, de Lencastre H, Bentley SD, Kearns AM, Holden MT (2017) Methicillin-resistant Staphylococcus aureus emerged long before the introduction of methicillin into clinical practice. Genome Biol 18-1-1.
  4. Gajdács M (2019) The continuing threat of methicillin-resistant Staphylococcus aureus. Antibiotics (Basel) 8(2):52. https://10.3390/antibiotics8020052
  5. Périchon B, Courvalin P (2009) VanA-type vancomycin-resistant Staphylococcus aureus. Antimicrob Agents  Chemother 53(11):4580-7.
  6. Shokoohizadeh L, Ekrami A, Labibzadeh M, Ali L, Alavi SM (2018) Antimicrobial resistance patterns and virulence factors of enterococci isolates in hospitalized burn patients. BMC Res Notes 11(1):1. https://10.1186/s13104-017-3088-5
  7. CLSI (2006) Autoverification of clinical laboratory test results; approved guideline (AUTO10-A). Wayne, PA, USA. https://clsi.org/media/1342/auto10a_sample.pdf
  8. Atshan SS, Shamsudin MN, Karunanidhi A, van Belkum A, Lung LT, Sekawi Z, et al. Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA). Infect Genet Evol 2013;18:106-12.
  9. Elhassan MM, Ozbak HA, Hemeg HA, Elmekki MA, Ahmed LM (2015) Absence of the mecA gene in methicillin resistant Staphylococcus aureus isolated from different clinical specimens in Shendi city, Sudan. Biomed Res Int 2015:895860.
  10. Fathali Z, Mirzaee M, Najarpeerayeh S (2016) Identification sec, hla, pvl and tsst-1 toxins genes profile in of methicillin-resistant Staphylococcus aureus clinical isolates. JIUMS, 24:32-40.
  11. Praharaj I, Sujatha S, Parija SC (2013) Phenotypic and genotypic characterization of vancomycin resistant Enterococcus isolates from clinical specimens. Indian J Med Res. 138(4):549-56.
  12. Purohit G, Gaind R, Dawar R, Verma PK, Aggarwal KC, Sardana R, et al (2017) Characterization of vancomycin resistant enterococci in hospitalized patients and role of gut colonization. J Clin Diagn Res 11(9):DC01-DC05.
  13. Tarai B, Das P, Kumar D (2013) Recurrent challenges for clinicians: emergence of methicillin-resistant Staphylococcus aureus, vancomycin resistance, and current treatment options. J Lab Physicians 5(2):71-8. https://10.4103/0974-2727.119843
  14. Al Bshabshe A, Joseph MRP, Awad El-Gied AA, Fadul AN, Chandramoorthy HC, Hamid ME (2020) Clinical relevance and antimicrobial profiling of methicillin-resistant Staphylococcus aureus (MRSA) on routine antibiotics and ethanol extract of Mango kernel (Mangifera indica L.). Biomed Res Int 2020:4150678. https://10.1155/2020/4150678
  15. Keneh NK, Kenmoe S, Bowo-Ngandji A, Tatah Kihla Akoachere JF, Gonsu Kamga H, Ndip RN, Ebogo-Belobo JT, Kengne-Ndé C, Mbaga DS, Tendongfor N, Ndip LM (2023) A mapping review of methicillin-resistant Staphylococcus aureus proportions, genetic diversity, and antimicrobial resistance patterns in Cameroon. Plos one 18(12):e0296267.
  16. Xing A, Ng HM, Jiao H, Li K, Ye Q (2024) The Prevalence, Epidemiological, and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) in Macau (2017–2022). Microorganisms 12(1):148.
  17. Congdon ST, Guaglione JA, Ricketts OM, Murphy KV, Anderson MG, Trowbridge DA, Al-Abduladheem Y, Phillips AM, Beausoleil AM, Stanley AJ, Becker TJ (2023) Prevalence and antibiotic resistance of Staphylococcus aureus associated with a college-aged cohort: Life-style factors that contribute to nasal carriage. Front Cell Infect Microbiol 13:1195758.
  18. Lee AS, de Lencastre H, Garau J, Kluytmans J, Malhotra-Kumar S, Peschel A, et al (2018) Methicillin-resistant Staphylococcus aureus. Nat Rev Dis Primers 4:18033.
  19. Huang SH, Chen YC, Chuang YC, Chiu SK, Fung CP, Lu PL, et al () Prevalence of vancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA among methicillin-resistant S. aureus with high vancomycin minimal inhibitory concentrations in Taiwan: A multicenter surveillance study, 2012-2013. J Microbiol Immunol Infect. 2016;49(5):701-707.
  20. Shariati A, Dadashi M, Moghadam MT, van Belkum A, Yaslianifard S, et al (2020) Global prevalence and distribution of vancomycin resistant, vancomycin intermediate and heterogeneously vancomycin intermediate Staphylococcus aureus clinical isolates: a systematic review and meta-analysis. Sci Rep 10(1):12689.
  21. Mollaei M, Rashki A (2016) The prevalence of adhesive surface encoding genes in Staphylococcus aureus isolated from hospitalized patients in Zabol-Iran by multiplex PCR. JABS 6:296-302.
  22. Nourbakhsh F, Momtaz H (2015) Detection of antibiotic resistance patterns in Staphylococcus aureus strains isolated from patients admitted to Isfahan hospitals during 2014-2015. Feyz 19(4):356-363.
  23. Khan S, Marasa BS, Sung K, Nawaz M (2021) Genotypic characterization of clinical isolates of Staphylococcus aureus from Pakistan. Pathog 10(8):918.
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