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Effect of tryptophan, phenylalanine and methionine amino acids on the morpho-physiological and phytochemical properties of Persian Gulnar (Punica granatum L. var. pleniflora) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 8, Volume 40, Issue 2 - Serial Number 120, June 2024, Pages 314-330 PDF (1.11 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2023.362422.3326 | ||
| Authors | ||
| F. Mohammadi Dolatabad1; M. Sanikhani* 1; M. Yaghoobi2; A. Kheyri3 | ||
| 1Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Iran | ||
| 2Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Zanjan, Iran | ||
| 3Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan,Iran | ||
| Abstract | ||
| Background and objectives: Punica granatum L. var. pleniflora is an ornamental/medicinal shrub native to Iran. Gulnar extract, like morphine, reduces pain in patients. It is also used against various diseases such as prostate cancer and breast cancer due to triterpenoids. In traditional medicine, it has been applied to cure diarrhea, bleeding, mucous secretions, and vaginal secretions. In external use, it has been used as a gargle to cure tonsillitis. Atherosclerosis is one of the most dangerous factors in cardiovascular disease. Due to antioxidant compounds, Gulnar reduces artery-clogging by almost 70%. Therefore, it is critical to enhance Persian Gulnar growth and flowering, a natural source of antioxidants. Methodology: To investigate the effect of the amino acids tryptophan, phenylalanine, and methionine on the morphophysiological and phytochemical characteristics of the Persian Gulanar, an experiment was conducted in the research greenhouse of Zanjan University in a completely randomized design including ten treatments in three replications and 3 pots for each experimental unit in the research greenhouse, Faculty of Agriculture of Zanjan University. Different levels of treatment included methionine 0.5, 1, 2 mM, phenylalanine 0.5, 1, 2 mM, tryptophan 0.5, 1, 2 mM. The extraction conditions were optimized for total phenol, total flavonoid, antioxidant activity, and antimicrobial activity tests. To extract and optimize the extraction using a dry flower sample, an ultrasonic device was used and an experiment was designed to determine the appropriate solvent, temperature, and duration of extraction, as well as to determine the optimal ratio of plant dry matter to solvent, and a comparison was made between two water solvents and alcohol at temperatures of 30-50-70 degrees Celsius and different amounts of plants 0.2, 0.35, and 0.5 grams per ten milliliters of solvent and durations of 5, 15, 25 minutes. Based on these cases, the initial tests by recommended software. To investigate the antimicrobial activity of the flower extract and Persian Gulnar's red color, a good penetration method was used. Plates containing a solid culture medium of Mueller Hinton agar were used in this method. Results: The treatments significantly affected the dry weight of flowers and flavonoids at a 5% probability level. In addition, they affected the number of flowers, anthocyanin content, antioxidant activity (FRAP), and chlorophyll b at a 1% probability level. The flower dry weight increased by 97.09% in the presence of 2 mM tryptophan compared to the control. The most anthocyanin was observed with an increase of 56.05 and 50% in the concentration of 0.5 mM phenylalanine compared to the control. The highest amount of antioxidant activity (FRAP) was obtained with a 110% increase compared to the control at a concentration of 2 mM tryptophan. The highest flower number was at 1 mM phenylalanine and 2 mM methionine. The most positive correlation (*r=0.68) was between flower dry weight and branch growth, and the most negative correlation (*r=0.80) was between chlorophyll b and anthocyanin. There was a significant positive correlation between chlorophyll a and total flavonoids, chlorophyll b, and branch growth. Also, there was a significant positive relationship between the number of flowers with anthocyanin and total phenol. The best extraction point was related to the temperature of 70 ºC, 70% ethanol solvent, 15 minutes, and the ratio of plant dry matter to solvent was 0.2 grams of dry weight in 10 ml of solvent. The red flowers showed a higher inhibitory effect against Escherichia coli, and the variegated flowers had a higher inhibitory power against Staphylococcus aureus. Conclusion: According to the results, in addition to confirming Gulnar's antibacterial activity and determining the optimal extraction point, it was found that the levels of 0.5 mM amino acids in most of the traits, including branch growth, flower dry weight, total phenol, and anthocyanin brought the best results from the treatment. It also justifies them in the field. Since amino acid fertilizers are applied in small amounts, they are economically justified. | ||
| Keywords | ||
| Anthocyanin; pomegranate; flower; phenolic content; foliar application | ||
| References | ||
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- Abd El-Aal, F.S., Shaheen, A.M., Ahmed, A.A. and Mahmoud, A.R., 2010. Effect of foliar application of urea and amino acids mixtures as antioxidants on growth, yield and characteristics of squash. Journal of Agriculture and Biological Scienc, 6(5): 583-588. - Ahmad, P., Alyemeni, M.N., Ahanger, M.A., Egamberdieva, D., Wijaya, L. and Alam, P., 2018. Salicylic acid (SA) induced alterations in growth, biochemical attributes and antioxidant enzyme activity in faba bean (Vicia faba L.) seedlings under NaCl toxicity. Russian Journal of Plant Physiology, 65: 104-114. - Ahmed, Y.M., Shalaby, E.A. and Shanan, N.T., 2011. The use of organic and inorganic cultures in improving vegetative growth, yield characters and antioxidant activity of roselle plants (Hibiscus sabdariffa L.). African Journal of Biotechnology, 10(11): 1988-1996. - Ali, K., Khan, S., Khan, N., Khan, W., Rahman, I.U., Ullah, F. and Nisar, M., 2017. Ethnobotanical and ecological study of Punica granatum in Dir district, Khyber Pakhtunkhwa, Pakistan. Regulatory Mechanisms in Biosystems, 4(8): 656-661. - Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy journal, 23(1): 112-121. - Asdagh, A. and Pirsa, S., 2020. Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/ β-carotene. International Journal of Biological Macromolecules, 165: 156-168. - Aviram, M., Volkova, N., Coleman, R., Dreher, M., Kesava, M., Ferreira, D. and Rosenblat, M., 2008. Pomegranate phenolics from the peels, arils, and flowers are antiatherogenic: studies in vivo in atherosclerotic apolipoprotein edeficient (E o) mice and in vitro in cultured macrophages and lipoproteins. Journal of Agriculture and Food chemistry, 56(3): 1148-1157. - Awad, E.M., Abd El-Hameed, A.M. and Shall, Z.S., 2007. Effect of glycine, lysine and nitrogen fertilizer rates on growth, yield and chemical composition of potato. Mansoura University Journal of Agriculture Sciences, 32(10): 8541-8551. - Balouiri, M., Sadiki, M. and Ibnsouda, S., 2016. Methods for in vitro evaluating antimicrobial activity. Journal of pharmaceutical analysis, 6(2): 71-79. - Baser, K.H.C., 2008. Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Journal of Current Pharmaceutical Design, 14: 3106-3119. - Chakraborthy, G.S., 2008. Analgesic activity of various extracts of Punica granatum L. flowers. International Journal of Green Pharmacy (IJGP), 2(3): 145-146. - Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3): 3. - Edreva, A., 2005. The importance of nonphotosynthetic pigments and cinnamic acid derivatives in photoprotection. Agriculture. Journal of Ecosystems and Environment, 106(1-2): 135-146. - El-Shabasi, M.S., Mohamed, S.M. and Mahfouz, S.A., 2005. Effect of foliar spray with amino acids on growth, yield and chemical composition of garlic plants. The 6th Arabian Conference for Horticulture, March 20-22, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt. - El-Din, K.M.G. and El-Wahed, M.S.A.A., 2005. Effect of some amino acids on growth and essential oil content of chamomile plant. International Journal of Agriculture and Biological Scienc, 7: 376-380. - Faten, S.A., Shaheen, A.M., Ahmad, A.A. and Mahmoud, A.R., 2010. Effect of foliar application of amino acids as antioxidants on growth, yield and characteristics of squash. Journal of Agriculture and Biological Scienc, 6(5): 583-588. - Garde-Cerdán,T., López, R., Portu, J., González-Arenzana, L., López-Alfaro, I. and Santamaría, P., 2014. Study of the effects of proline, phenylalanine, and urea foliar application to Tempranillo vineyards on grape amino acid content. Comparison with commercial nitrogen fertilisers. Journal of Food chemistry, 163: 136-141. - Gharib, S.A., El-Mogy, M.M., Gawad, A. and Shalaby, E.A., 2011. Influence of compost, amino and humic acids on the growth, yield and chemical parameters of strawberries. Journal of Medicinal Plants Research, 5(11): 2304-2308. - Ghasemzadeh, A., Jaafar, H.Z. and Karimi, E., 2012. Involvement of salicylic acid on antioxidant and anticancer properties, anthocyanin production and chalcone synthase activity in ginger (Zingiber officinale R.) varieties. International journal of molecular sciences, 13(11): 14828-14844. - Hassani Moghadam, E., Shaaban, M. and Sepahvand, A., 2019. Medicinal properties of Pomegranate. Herbal Medicines Journal, 4(3): 127-139. - Hassoun, A., Carpena, M., Prieto, M.A., Simal-Gandara, J., Özogul, F., Özogul, Y., Çoban, Ö.E., Gudjónsdóttir, M., Barba, F.J., Marti-Quijal, F.J., Jambrak, A.R., Maltar-Strmečki, N., Kljusurić, J.G. and Regenstein, J.M., 2020. Use of Spectroscopic Techniques to Monitor Changes in Food Quality during Application of Natural Preservatives: A Review. Antioxidants, 9(9): 882. - Hatamian, M., Arab, M. and Roozban, M.R., 2014. Photosynthetic and nonphotosynthetic pigments of two rose cultivars under different light intensities. Journal of Agricultural crop management, 4(16): 259-270. - Jaime, A., Silva, T.D., Rana, T.S., Narzary, D., Verma, N., Meshram, D.T. and Ranade, S.A., 2013. Pomegranate biology and biotechnology: a review. Scientia Horticulturae, 160: 85-107. - Karima, M., Gamal EL-din, M. and Abdel -Wahed, M.S.A., 2005. Effect of Some Amino Acids on Growth and Essential Oil Content of Chamomile Plant. International Journal of Agriculture and Biological Scienc, 3: 376-380. - Kiaei E, Mazandarani M, Ghaemi E. 2010. Antibacterial activity of 7 species of medicinal plants on bacteria isolated from UTI patients in Golestan province. Journal of medicinal plants. 2010; 9(34): 74-83. - Mervat, Sh.S., Salva, A.O. and Bakry, A.B., 2015. Antioxidant properties, secondary metabolites and yield as affected by application of antioxidants and banana peel extract on Roselle plants. American-Eurasian Journal of Sustainable Agriculture, 9(4): 93-104. - Mustafa, A., Imran, M., Ashraf, M. and Mahmood, K., 2018. Perspectives of using l-tryptophan for improving productivity of agricultural crops: A review. Pedosphere, 28(1): 16-34. - Nahed, G.A.A., Lobna, S.T. and Soad, M.I., 2009. Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus plants at Nubaria. Ozean Journal of Applied Science, 2(2): 169-179. - Nahed, G., Abdel Aziz, A., Mazher, A.M. and Farahat, M.M. 2010. Response of vegetative growth and chemical constituents of Thuja orientalis L. plant to foliar application of different amino acids at Nubaria. Journal of American Science, 6(3): 295-301. - Neeraja, G., Reddy, I. and Gautham, B., 2005. Effect of growth promoters on growth and yield of tomato cv. Marutham. Journal of Research ANGRAU, 33(3): 68-70. - Pandjaitan, N., Howard, L.R., Morelock, T. and Gil, M.I., 2005. Antioxidant capacity and phenolic content of spinach as affected by genetics and maturation. Journal of Agricultural and Food Chemistry, 53(22): 8618-8623. - Pinton, R., Cesco, S., Santi, S., Agnolon, F. and Varanini, Z., 1999. Water extractable humic substances enhance iron deficiency responses by Fedeficient cucumber plants. Journal of Plant and Soil, 210: 145-157. - Portu, J., Gonzalez-Arenzana, L., Hermosin-Gutierrez, I., Santamaria, P. and Garde-Cerdan, T., 2015. Phenylalanine and urea foliar applications to grapevine: Effect on wine phenolic content. Journal of Food Chemistry, 180: 55-63. - Proestos, C., Boziaris, I.S., Nychas, J.E. and Komaitis, M., 2006. Analysis of flavonoids and phenolic acids in Greek aromatic plants: Investigation of their antioxidant capacity and antimicrobial activity. Journal of Food Chemistry, 95: 664-671. - Reham, M.S., Khattab, M.E., Ahmed, S.S. and Kandil, M.A.M., 2016. Influence of foliar spray with phenylalanine and nickel on growth, yield quality and chemical composition of genoveser basil plant. African Journal of Agricultural Research, 11(16): 1398-1410. - Rezakhani, A. and Hadi, M.R.H.S., 2017. Effect of manure and foliar application of amino acids on growth characteristics, seed yield and essential oil of coriander (Coriandrum sativum L.). Iranian Journal of Field Crop Science, 48(3): 777-786. - Shehata, S.M., Abdel-Azem, H.S., El-Yazied, A.A. and El-Gizawy, A.M., 2011. Effect of foliar spraying with amino acids and seaweed extract on growth chemical constitutes, yield and its quality of celeriac plant. European Journal of Scientific Research, 58(2): 257-265. - Singh, R., Singh, S., Kumar, S. and Arora, S., 2007. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Journal of Food and chemical toxicology, 45(7): 1216-1223. - Stanković, N., Mihajilov-Krstev, T., Zlatković, B., Stankov-Jovanović, V., Mitić, V., Jović, J. and Bernstein, N., 2016. Antibacterial and antioxidant activity of traditional medicinal plants from the Balkan Peninsula. NJAS-Wageningen Journal of Life Sciences, 78: 21-28. - Swetie, R., Raesh, Ch. and Arun, S., 2007. Antioxidant potential of mint (Mentha Spicata L.) in radiationprocessed lamb meat. Journal of Food Chemistry, 100(2): 451-458. - Syed, D.N., Afaq, F and Mukhtar, H., 2007. Pomegranate derived products for cancer chemoprevention. Phytonutrients, 17: 377-385. - Tasdelen, F.N., Tanriverdi, C.Y., Coban, A.Y., Ozatli, D., Tanyel, E., Durupinar, B. and Tulek, N., 2009. Antimicrobial activity of plant extracts Ankaferd Blood Stopper. Fitoterapia, 80(1): 48-50. - Teixeira, W.F., Fagan, E.B., Soares, L.H., Soares, J.N., Reichardt, K. and Neto, D.D., 2018. Seed and foliar application of amino acids improve variables of nitrogen metabolism and productivity in soybean crop. Frontiers in Plant Science, 9: 396. - Tzin, V. and Galili, G., 2010. The biosynthetic pathways for shikimate and aromatic amino acids in Arabidopsis thaliana. The Arabidopsis book/American Society of Plant Biologists, 8p. - Vučić, V., Grabež, M., Trchounian, A. and Arsić, A., 2019. Composition and potential health benefits of pomegranate: a review. Current pharmaceutical design, 25(16): 1817-1827. - Wagner, G.J., 1979. Content and vacuole/extra vacuole distribution of neutral sugars free amino acid, and anthocyanins in protoplast. Journal of Plant physiology, 64: 88-93. - Wahba, H.E., Motawe, H.M. and Ibrahim, A.Y., 2015. Growth and chemical composition of Urtica pilulifera L. plant as influenced by foliar application of some amino acids. Journal of Materials Environmental Science, 6(2): 499-506. - Youssef, A.A., Khattab, M.E. and Omer, E.A., 2004. Effect of spraying of molybdenum and tyrosine on growth, yield and chemical composition of lemon basil plant. Egyptian Pharmaceutical Journal, 3(2): 87-106. - Zamani, S., Kazemi, M. and Aran M., 2011. Postharvest life of vut rose flowers as effected by salicylic acid and glutamin. World Applied Science Journal, 12(9): 1621-1624. | ||
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