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Edrisi, Mehdi, Safi, Shahabeddin, Varshovi, Hamid Reza, Shahhosseiny, Mohammad Hassan. (1403). Evaluation and optimization of loop-mediated isothermal amplification (LAMP) technique in capripoxvirus diagnosis and its comparison with PCR method. سامانه مدیریت نشریات علمی, 79(6), 1183-1190. doi: 10.32592/ARI.2024.79.6.1183
Mehdi Edrisi; Shahabeddin Safi; Hamid Reza Varshovi; Mohammad Hassan Shahhosseiny. "Evaluation and optimization of loop-mediated isothermal amplification (LAMP) technique in capripoxvirus diagnosis and its comparison with PCR method". سامانه مدیریت نشریات علمی, 79, 6, 1403, 1183-1190. doi: 10.32592/ARI.2024.79.6.1183
Edrisi, Mehdi, Safi, Shahabeddin, Varshovi, Hamid Reza, Shahhosseiny, Mohammad Hassan. (1403). 'Evaluation and optimization of loop-mediated isothermal amplification (LAMP) technique in capripoxvirus diagnosis and its comparison with PCR method', سامانه مدیریت نشریات علمی, 79(6), pp. 1183-1190. doi: 10.32592/ARI.2024.79.6.1183
Edrisi, Mehdi, Safi, Shahabeddin, Varshovi, Hamid Reza, Shahhosseiny, Mohammad Hassan. Evaluation and optimization of loop-mediated isothermal amplification (LAMP) technique in capripoxvirus diagnosis and its comparison with PCR method. سامانه مدیریت نشریات علمی, 1403; 79(6): 1183-1190. doi: 10.32592/ARI.2024.79.6.1183

Evaluation and optimization of loop-mediated isothermal amplification (LAMP) technique in capripoxvirus diagnosis and its comparison with PCR method

Archives of Razi Institute
مقاله 7، دوره 79، شماره 6، بهمن و اسفند 2024، صفحه 1183-1190    اصل مقاله (329.04 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.6.1183
نویسندگان
Mehdi Edrisi1؛ Shahabeddin Safi* 1؛ Hamid Reza Varshovi2؛ Mohammad Hassan Shahhosseiny3
1Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
چکیده
Sheep pox (SP), goat pox (GP), and lumpy skin disease (LSD), subspecies of the Capripox virus (CaPVs), are important in the pathogenesis of sheep, goats, and cattle. The causative agent is the capripox virus which has been isolated in South Africa for the first time. Sheeppox (SP), goatpox (GP), and lumpy skin disease (LSD) viruses are morphologically indistinguishable from each other and have been adapted to different host species (4). From a serological point of view, it is difficult to differentiate these viruses and cross-immunity occurs among them (2). The present study reports the evaluation and optimization of a novel loop-mediated isothermal amplification (LAMP) technique for the rapid detection of CaPVs and compares the LAMP technique with the PCR method. LAMP primers were selected from the P32-protected gene of the Capripox virus. Safe-Red fluorescent dye was used to monitor and change the color in the disease’s positive cases to bright yellow at a 320-nm wavelength, and the final results were confirmed using electrophoresis. The proposed LAMP test for the Capripox virus showed high specificity and non-cross-reactivity with viruses of Poxviridae with similar clinical symptoms. The optimized LAMP test was compared with PCR. LAMP and PCR were similar in diagnostic sensitivity. The specificity was evaluated using 30 samples of cow skin suspected of having lumpy skin disease as well as 16 samples of positive and negative references and the negative control. The proposed easy-to-use, inexpensive, high-sensitivity LAMP test might be suitable for detecting caprypox virus in laboratories in border areas and rural areas.
کلیدواژه‌ها
LSDV؛ LAMP؛ PCR؛ Capripoxvirus
عنوان مقاله [English]
ارزیابی و بهینه سازی تکنیک Loop-mediated isothermal amplification (LAMP) درتشخیص capripoxvirus و مقایسه آن با روش PCR
چکیده [English]
آبله گوسفند (SP) ، آبله بز(GP) و بیماری لمپی اسکین (LSD) از زیر گونه capripoxvirus (CaPVs) می باشند که اهمیت اقتصادی در بیماری زایی گوسفند ، بز و گاو برعهده دارند. ما در اینجا ارزیابی و بهینه سازی تکنیک بی همتای LAMP را برای تشخیص سریع کاپری پاکس ویروس و مقایسه آن با روش PCR را گزارش می کنیم. پرایمرهای LAMP از ژنوم محافظت شده P32 از این جنس انتخاب شدند. رنگ فلوروسنتی SYBR safe برای مانیتورینگ و ایجاد تغییر رنگ در موارد مثبت بیماری به سبز درخشان در طول موج 350 نانومتر بکار گرفته شد و نتایج نهایی با الکتروفورزیس تأیید شد. تست LAMP با ویژگی بالا برای capripoxvirus و عدم واکنش متقاطع حتی با ویروس های خانواده پاکس ویریده و یا ویروس های با علایم کلینیکی مشابه ارزیابی شد. تست LAMP بهینه شده با روش PCR مقایسه شد. LAMP و PCR از لحاظ حساسیت تشخیصی مشابه و با محدوده تشخیص LOD 3 و 8 کپی ژنوم می باشد. ویژگی تشخیصی با تعداد 30 نمونه پوست گاو مشکوک به بیماری لمپی اسکین و 16 عدد نمونه رفرانس مثبت و منفی و کنترل منفی ارزیابی شد . تست LAMP با قابلیت استفاده آسان ، ارزان با حساسیت بالا و بخصوص مناسب برای تشخیص کاپری پاکس ویروس در آزمایشکاه های مناطق مرزی و آزمایشگاه های مناطق روستایی، در این گزارش توصیف می شود.
کلیدواژه‌ها [English]
ویروس لمپی اسکین, LAMP, واکنش زنجیره ای پلمیراز, کاپری پاکس ویروس
مراجع
  1. Babiuk S, Bowden TR, Boyle DB, Wallace DB, Kitching RP. Capripoxviruses: An Emerging Worldwide Threat to Sheep, Goats and Cattle. Transboundary and Emerging Diseases. 2008; 55(7):263–72.
  2. Balinsky CA, Delhon G, Smoliga G, Prarat M, French RA, Geary SJ, et al. Rapid Preclinical Detection of Sheeppox Virus by a Real-Time PCR Assay. Journal of Clinical Microbiology. 2008;46(2):438–42.
  3. Bowden TR., Babiuk SL, Parkyn GR, Copps JS, Boyle DB. Capripoxvirus tissue tropism and shedding: A quantitative study in experimentally infected sheep and goats. Virology. 2008;371(2):380–393.
  4. Das A, Babiuk S, McIntosh MT. Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses. Journal of Clinical Microbiology. 2012;50(5):1613–20.
  5. Heine HG, Stevens MP, Foord AJ, Boyle DB. A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. Journal of Immunological Methods. 1999;227(1-2):187–96.
  6. Lamien CE, Lelenta M, Goger W, Silber R, Tuppurainen E, Matijevic M, et al. Real time PCR method for simultaneous detection, quantitation and differentiation of capripoxviruses. Journal of Virological Methods. 2011; 171(1):134–40.
  7. Tuppurainen ESM, Oura CAL. Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia. Transboundary and Emerging Diseases. 2011;59(1):40–8.
  8. Salem H. Molecular characterization of lumpy skin disease viurs in cattle. Egyptian Journal of Animal Health. 2021;1(1):1–19.
  9. Woods JA. Lumpy skin disease—A review. Tropical Animal Health and Production. 1988; 20(1):11–7.
  10. Agag BI, Mousa Sh, Hassan HB, Saber MS, El-Deghidy NS, El-Aziz AMAbd. Clinical, Serological and Biochemical Studies on Lumpy Skin Disease. Journal of Applied Animal Research. 1992; 1(1):13–23.
  11. Wijesiriwardana N, Haga I, Sanz-Bernardo B, Graham S, Beard P. Characterising the cell-mediated immune response to Lumpy skin disease virus. Access Microbiology. 2019;1(1A).
  12. Samojlović M, Polaček V, Gurjanov V, Lupulović D, Lazić G, Petrović T, et al. Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods. Acta Veterinaria. 2019;69(1):47–60.
  13. Tabatabaei M, Mohammadi A, Varshovi HR. Molecular characterization of lumpy skin disease virus in Iran (2014–2018). Archives of Virology. 2021;166(8):2279-2283.
  14. Murray L, Edwards L, Tuppurainen ES, Bachanek-Bankowska K, Oura CA, Mioulet V, et al. Detection of capripoxvirus DNA using a novel loop-mediated isothermal amplification assay. BMC Veterinary Research. 2013; 9(1):90.
  15. Tanner NA, Evans TC. Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids. Current Protocols in Molecular Biology. 2014; 105(1):15.14.1-14.
  16. Hara-Kudo Y, Yoshino M, Kojima T, Ikedo M. Loop-mediated isothermal amplification for the rapid detection of Salmonella. FEMS Microbiology Letters. 2005;253(1):155–61.
  17. Tomita N, Mori Y, Kanda H, Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nature Protocols. 2008;3 (5):877–82.
  18. Mori Y, Notomi T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. Journal of Infection and Chemotherapy. 2009; 15(2):62–9.
  19. Goto M, Honda E, Ogura A, Nomoto A, Hanaki K-I. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. BioTechniques. 2009;46(3):167–72.
  20. Blomström A-L, Hakhverdyan M, Reid SM, Dukes JP, King DP, Belák S, et al. A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. Journal of Virological Methods. 2008; 147(1):188–93.
  21. Notomi T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Research. 2000; 28(12):63e63.
  22. Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T. Detection of Four Plasmodium Species by Genus- and Species-Specific Loop-Mediated Isothermal Amplification for Clinical Diagnosis. Journal of Clinical Microbiology. 2007;45(8):2521-8.
  23. Wang F, Jiang L, Ge B. Loop-Mediated Isothermal Amplification Assays for Detecting Shiga Toxin-Producing Escherichia coli in Ground Beef and Human Stools. Journal of Clinical Microbiology. 2012; 50(1):91–7.
  24. Hamed Isapour, Mehdi Sakha, Hamid Reza Varshovi. Comparative Investigation of Clinical Findings and Epidemiologic Indices of Lumpy Skin Disease Between Native and Holstein Cattle Breeds. DOAJ (DOAJ: Directory of Open Access Journals). 2021;15(3):287-294.
  25. Arash Ghalyanchilangeroudi, Zahra Ziafati Kafi, Rajeoni A, Jamil Ataii, Sadri N, Niusha Hajizamani, et al. Molecular Detection and Phylogenetic Analysis of Lumpy Skin Disease Virus in Iran. DOAJ (DOAJ: Directory of Open Access Journals). 2021;15(2):169-173.
  26. Roya Sadri. Prevalence and economic significance of goat pox virus disease in semi-arid provinces of Iran. Iranian Journal of Veterinary Medicine. 2012;6(3):187–90.
  27. Roya Sadri, Rozbeh Falahi. A new approach to develop a vaccine against capripox infection in sheep and goats using a new strain of sheep pox virus in Iran. Iranian Journal of Veterinary Medicine. 2010;4(4):391317.
آمار
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