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شناسایی و آنالیز ژن‌های کلیدی و مسیرهای سیگنالدهی دخیل در قطر پشم گوسفند | ||
| علوم دامی | ||
| دوره 37، شماره 142، خرداد 1403، صفحه 33-44 اصل مقاله (1.49 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2023.361244.2288 | ||
| نویسندگان | ||
| زهرا رودباری* 1؛ سعیده اسکندری نسب سیاهکوهی2 | ||
| 1دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران. | ||
| 2دانشجوی دکتری گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران. | ||
| چکیده | ||
| پشم گوسفند یک ماده خام بسیار مهم برای صنعت نساجی است. از آنجایی که قطر الیاف یکی از مهم‌ترین ویژگی‌های اقتصادی پشم گوسفند است، شناسایی ژن‌های تنظیم‌کننده این ویژگی فرصتی را برای افزایش بهره‌وری و بهبود کیفیت و تنوع محصول ارائه می‌دهد. پژوهش‌های مختلفی صورت گرفته و ژن‌های مختلفی در رابطه با الیاف تولیدی شناسایی شده است. هر ژن ممکن است در مسیرهای زیستی مختلفی دخیل باشد که شناسایی این مسیرهای زیستی و ژن‌ها تشکیل دهنده آن دیدگاه وسیع‌تر و فهم کامل‌تری در مورد مکانیسم ژنتیکی پیچیده صفات تولیدی ایجاد می کند که می‌تواند از طریق شناسایی نشانگرهای زیستی برای صفات تولیدی قدم مفیدی در جهت بهبود اصلاح نژاد باشد. داده‌های مورد استفاده در این پژوهش از پایگاه داده‌ای GEO با شماره دسترسی GSE85844 دانلود شدند و به منظور سنجش کیفیت و یک دست بودن مورد بررسی قرار گرفتند. جهت شناسایی ژن‌ها با بیان متفاوت حد آستانه‌ای در نظر گرفته شده که شامل P-value و Fold Change می‌باشد. جهت ترسیم شبکه و آنالیز هستی‌شناسی از نرم‌افزارString و آنالیز شبکه از نرم‌افزار Cytoscape افزونه cytoHubba استفاده شد. در مجموع 702 ژن با بیان متفاوت شناسایی شد که در 37 مسیربیولوژیکی مرتبط با تولید فولیکول‌های پشم دخیل هستند. نتایج آنالیز شبکه 11 ژن بزرگ اثر را شناسایی کرد که بر رشد و قطر پشم تاثیر دارند. این نتایج منابع ارزشمندی را برای افزایش کیفیت و تولید پشم گوسفند فراهم می کند. | ||
| کلیدواژه‌ها | ||
| الیاف؛ بیان ژن؛ مسیرسیگنالیک؛ شبکه ژنی؛ قطرپشم | ||
| عنوان مقاله [English] | ||
| Identification and analysis of key genes and signaling pathways involved in sheep wool diameter | ||
| نویسندگان [English] | ||
| Zahra Roudbari1؛ Saideh EskandariNasab2 | ||
| 1Department of animal science, faculaty of Agriculture, Jiroft university, Jiroft,Iran | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Zabol. Zabol. Iran. | ||
| چکیده [English] | ||
| Sheep wool is a very important raw material for the textile industry. Since fiber diameter is one of the most important economic characteristics of sheep wool, identification of genes regulating this characteristic offers an opportunity to increase productivity and improving product quality and variety of product. various researches have been conducted and various genes have been identified in relation to wool production. Each gene may be involved in different biological pathways, and the identification of these biological pathways and the genes that make up them will create a broader view and a more complete understanding of the complex genetic mechanism of the phenotype of productive traits, which can be achieved through the identification of biological biomarkers. For production traits, it is a useful step toward improving breeding. The data used in this research were downloaded from the GEO database with the access number GSE85844 and were analyzed in order to measure quality and uniformity. In order to identify genes with expression differences, a series of thresholds were considered, which include P-value and Fold Change. String software was used for ontology analysis and Cytoscape software with the cytoHubba plugin was used for network analysis. A total of 702 genes with different expressions were identified, which are involved in 37 biological pathways related to the production of wool follicles. The results of the network analysis identified 11 hub genes that affect wool growth and diameter. These results provide valuable resources for increasing the quality and production of sheep wool. | ||
| کلیدواژه‌ها [English] | ||
| wool, expression profile, signaling pathway, Gene network, Diameter Wool | ||
| مراجع | ||
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