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Abdalfatah, Mariwan Fathalla, Hjazi, Ahmed, Saravani, Khadijeh, Hassanshahian, Mehdi, Bayat, Zeynab, Soheil beigie, Gelareh. (1403). Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section. سامانه مدیریت نشریات علمی, 80(1), 147-152. doi: 10.32592/ARI.2025.80.1.147
Mariwan Fathalla Abdalfatah; Ahmed Hjazi; Khadijeh Saravani; Mehdi Hassanshahian; Zeynab Bayat; Gelareh Soheil beigie. "Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section". سامانه مدیریت نشریات علمی, 80, 1, 1403, 147-152. doi: 10.32592/ARI.2025.80.1.147
Abdalfatah, Mariwan Fathalla, Hjazi, Ahmed, Saravani, Khadijeh, Hassanshahian, Mehdi, Bayat, Zeynab, Soheil beigie, Gelareh. (1403). 'Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section', سامانه مدیریت نشریات علمی, 80(1), pp. 147-152. doi: 10.32592/ARI.2025.80.1.147
Abdalfatah, Mariwan Fathalla, Hjazi, Ahmed, Saravani, Khadijeh, Hassanshahian, Mehdi, Bayat, Zeynab, Soheil beigie, Gelareh. Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section. سامانه مدیریت نشریات علمی, 1403; 80(1): 147-152. doi: 10.32592/ARI.2025.80.1.147

Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section

Archives of Razi Institute
مقاله 17، دوره 80، شماره 1، فروردین و اردیبهشت 2025، صفحه 147-152    اصل مقاله (398.05 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.1.147
نویسندگان
Mariwan Fathalla Abdalfatah1؛ Ahmed Hjazi2؛ Khadijeh Saravani3؛ Mehdi Hassanshahian* 4؛ Zeynab Bayat4؛ Gelareh Soheil beigie5
1Department of Medical Laboratory, Kurdistan Technical Institute, Sulaymaniyah, Kurdistan Region, Iraq
2PhD in Clinical Medicine, Department of Medical Laboratory, College of Applied Medical Sciences, Prince Stattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia
3Department of community Medicine, School of Medicine, Pediatric Gastroenterology and Hepatology Research Center, Zabol University of Medical Science, Zabol, Iran
4Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
5PhD Student in microbiology, Islamic Azad University of Ayatollah Amoli, Amol, Iran
چکیده
The corona pandemic caused a large number of people to be admitted to the intensive care units of hospitals. Due to the special closed system of these parts and the lack of proper air circulation, Spire equipment is a suitable place for the accumulation of commensal bacteria and the creation of biofilm. Biofilm is actually a collection of microbes that stabilize on a surface. Bacteria in biofilm structures have relationships with each other and their behavior is different from when they are alone. The resistance of bacteria to antimicrobial agents in the biofilm state is different from the planktonic state. Many genes are involved in the formation of biofilm, these genes act in a cascade manner and the activity of one gene depends on the activity of another gene
Acinetobacter, recognized as a nosocomial pathogen, undergoes structural changes when exposed to various antibiotics, rendering it relatively resistant and posing challenges in disease treatment. This study aimed to identify two biofilm-related genes and assess the drug resistance profile of clinical strains. Clinical isolates were collected from the ICU of Afzalipour Hospital in Kerman, Iran, and phenotypically identified. Confirmation was achieved for 55 clinical Acinetobacter isolates. Antibiogram testing was conducted for meropenem, amikacin, ampicillin-sulbactam, cefotaxime, levofloxacin, rifampin, and tigecycline antibiotics. Biofilm formation ability was assessed using microtiter plates and crystal violet staining, followed by spectrophotometry at OD 490 nm. PCR was employed to determine the frequency of pslA and pelB genes. Analysis revealed that the highest age group affected was 1 to 15 years (19%), while the lowest was 26 to 35 years (5%). The frequencies of pslA and pelB genes were 34.5% and 65.5%, respectively, and drug resistance ranged from 72% to 100% for the mentioned antibiotics. Given the pelB gene's approximately twofold higher frequency compared to pslA, it suggests that in most studied isolates, Psl may often be disrupted or that intracellular c-di-GMP levels have significantly increased.
کلیدواژه‌ها
Biofilms؛ Acinetobacter؛ Drug Resistance؛ Antibiotic؛ pslA and pelB genes
عنوان مقاله [English]
غربالگری ژن‌های تولیدکننده بیوفیلم از ایزوله‌های اسینتوباکتر به‌دست‌آمده از بیماران کووید-19 در بخش ICU بیمارستان
چکیده [English]
سابقه و هدف: اسینتوباکتر که یک عامل عفونی بیمارستانی محسوب می شود، به دلیل قرار گرفتن در معرض آنتی بیوتیک های مختلف، ساختار خود را تغییر داده و نسبت به آنها مقاوم می شود که درمان بیماری های ناشی از این باکتری را دشوار می کند.
اپیدمی کرونا که از سال 2019 در جهان آغاز شد، منجر به افزایش تعداد بیماران در بخش ICU بیمارستان ها شد. ویروس کرونا به دلیل درگیری دستگاه تنفسی مشکلات زیادی را برای بیماران ایجاد کرد
هدف: این مطالعه با هدف شناسایی دو ژن سازنده بیوفیلم و همچنین مشخصات مقاومت دارویی سویه های بالینی انجام شد.
روش کار: در این مطالعه، ایزوله های بالینی از بیمارستان افضلی پور کرمان جمع آوری و به عنوان فنوتیپی شناسایی شدند. 55 ایزوله بالینی اسینتوباکتر تایید شد. آزمایش آنتی بیوگرام برای آنتی بیوتیک های مروپنم، آمیکاسین، آمپی سیلین-سولباکتام، سفوتاکسیم، لووفلوکساسین، ریفامپین و تیژسیکلین انجام شد. توانایی تشکیل بیوفیلم جدایه ها با استفاده از پلیت میکروتیتر و رنگ آمیزی با استفاده از کریستال ویولت و سپس اسپکتروفتومتری در OD 490 نانومتر تعیین شد. PCR برای شناسایی فراوانی ژن های pslA و pelB انجام شد.
یافته ها: داده ها نشان داد که بیشترین گروه سنی 1 تا 15 سال شامل 19 درصد و کمترین گروه سنی 26 تا 35 سال شامل 5 درصد بود. فراوانی ژن های pslA و pelB به ترتیب 5/34 و 5/65 درصد و دامنه مقاومت دارویی برای آنتی بیوتیک های مذکور بین 72 تا 100 درصد بود.
نتیجه‌گیری: از آنجایی که فراوانی ژن pelB حدود دو برابر pslA است، می‌توان نتیجه گرفت که در اکثر جدایه‌های مورد مطالعه، Psl اغلب دارای اختلال است یا سطح داخل سلولی c-di-GMP به شدت افزایش یافته است. .
کلیدواژه‌ها [English]
بیوفیلم, اسینتوباکتر, مقاومت دارویی, انتی بیوتیک, ژنهای PslA
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