| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,538 |
| تعداد مشاهده مقاله | 49,418,876 |
| تعداد دریافت فایل اصل مقاله | 79,860,807 |
Phenotypic and molecular characterization of Quambalaria cyanescens from walnut kernels infested with codling moth (Cydia pomonella) in Iran | ||
| Mycologia Iranica | ||
| دوره 10، شماره 2، اسفند 2023، صفحه 131-140 اصل مقاله (1.36 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/MI.2024.365215.1278 | ||
| نویسندگان | ||
| Zahra Mahdizadeh؛ Abolfazl Narmani؛ Mahdi Arzanlou* | ||
| Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| چکیده | ||
| In a survey of fungal species associated with walnut kernel rot symptoms, a white fungal mycelial mass was observed in feces and larval debries of codling moths (Cydia pomonella) on walnut kernels in East and West Azerbaijan provinces in 2022. Infected samples were examined under a stereo microscope, white mycelial mass together with fungal spores were taken using a sterile needle, and pure cultures were established using the single spore method. Morphological characteristics were examined on potato dextrose agar (PDA) and malt extract agar (MEA) culture media in the dark at 21°C after one week of incubation. To confirm the identity of the isolated fungi, the ITS rDNA genomic region of representative isolates were amplified using a universal primer set (ITS1 and ITS4) via polymerase chain reaction, and PCR products were sequenced. Based on the combination of morphological features and sequencing data, the isolates were identified as Quambalaria cyanescens. Colonies grew slowly, reaching a diameter of 11-12 mm on PDA and MEA after one week, and produced a purple pigment in the medium. Conidiophores are undifferentiated from vegetative hyphae and conidiogenous cells are holoblastic with sympodial proliferation. The conidia are usually ovoid or pear-shaped, transparent and 2-8 × 1.5-2.5 µm. Quambalaria cyanescens is a rare basidiomycete species of the order Microstromatales, which also has a yeast phase. The pathogenic potential of the two isolates of Q. cyanescens was evaluated on larvae of Ephestia kuehniella; however, the survival rate of larvae treated with different concentrations of Q. cyanescens spores was the same as that of untreated control larvae, and it can be concluded that the Q. cyanescens isolates were not pathogenic to E. kuehniella larvae. To the best of our knowledge, this is the first report on the association of Q. cyanescens with feces and larval debries of codling moths on walnut kernels. | ||
| کلیدواژهها | ||
| Eucalyptus؛ Quambalariaceae؛ Inesct damage؛ Mold contamination | ||
| مراجع | ||
|
Baradaran Bagheri, M., Arzanlou, M. and Babai-ahari, A. 2015. Identification of the fungal agents associated with almond trunk diseases in East Azerbeijan province. Applied Research in Plant Protection 4: 27–41.
Bezerra, J.D.P., Machado, A.R., Firmino, A.L., Rosado, A.W.C., Souza, C.A.F.D., Souza-Motta, C.M.D., Freire, K.T.L.D.S., Paiva, L.M., Magalhães, O.M.C., Pereira, O.L. and Crous, P.W. 2018. Mycological diversity description I. Acta Botanica Brasilica 32: 656–666.
Crous, P.W., Wingfield, M.J., Cheewangkoon, R., Carnegie, A.J., Burgess, T.I., Summerell, B.A., Edwards, J., Taylor, P.W.J. and Groenewald, J.Z. 2019. Foliar pathogens of eucalypts. Studies in Mycology 94: 125–298.
Campbell, B.C., Molyneux, R.J. and Schatzki, T.F. 2003. Current research on reducing pre‐and post‐harvest aflatoxin contamination of US almond, pistachio, and walnut. Journal of Toxicology. Toxin Reviews 22: 225–66.
Duong, H,T,, Mazanec, R., McComb, J.A., Burgess, T. and Hardy, G.E. 2022. Quambalaria shoot blight resistance in marri (Corymbia calophylla): genetic parameters and correlations between growth rate and blight resistance. Tree Genetics & Genomes. 18: 8.
de Beer, Z.W., Begerow, D., Bauer, R., Pegg, G.S., Crous, P.W. and Wingfield, M.J. 2006. Phylogeny of the Quambalariaceae fam. nov., including important Eucalyptus pathogens in South Africa and Australia. Studies in Mycology 55: 289–298.
de Hoog, G.S. and de Vries, G.A. 1973. Two new species of Sporothrix and their relation to Blastobotrys nivea. Antonie van Leeuwenhoek 39: 515–520.
FAO (Food and Agriculture Organization of the United Nations). 2023. FAO Statistical Databases.
Kari Dolatabad, H., Asadi Rahmani, H. and Rejali, F. 2019. Identification and evaluation of growth promoting and biocontrol properties of isolated endophytic fungi from the leaves and fruits of Pistacia vera. Journal of Soil Biology 7: 53–71.
Kolarik, M., Slavikova, E. and Pazoutova, S. 2006. The taxonomic and ecological characterisation of the clinically important heterobasiodiomycete Fugomyces cyanescens and its association with bark beetles. Czech Mycology 58:81.
Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874.
Middelhoven, W.J., Guého, E. and De Hoog, G.S. 2000. Phylogenetic position and physiology of Cerinosterus cyanescens. Antonie van Leeuwenhoek 77: 313–320.
Narmani, A., and Arzanlou, M. 2019. Quambalaria cyanescens, a new fungal trunk pathogen associated with grapevine decline in Iran. Crop Protection 124: 104875.
Oliveira, I,, Pereira, J.A., Lino-Neto, T., Bento, A. and Baptista, P. 2012. Fungal diversity associated to the olive moth, Prays oleae Bernard: a survey for potential entomopathogenic fungi. Microbial Ecology 63: 964–974.
Paap, T., Burgess, T.I., McComb, J.A., Shearer, B.L., and Hardy, G.E.S.J. 2008. Quambalaria species, including Q. coyrecup sp. nov., implicated in canker and shoot blight diseases causing decline of Corymbia species in the southwest of Western Australia. Mycological Research 112: 57–69.
Preto, G., Martins, F., Pereira, J.A., and Baptista, P. 2017. Fungal community in olive fruits of cultivars with different susceptibilities to anthracnose and selection of isolates to be used as biocontrol agents. Biological Control 110: 1–9.
Prochazkova, E., Kucherak, O., Stodůlková, E., Tošner, Z., Cisarova, I., et al. 2020. NMR Structure elucidation of Naphthoquinones from Quambalaria cyanescens. Journal of Natural Products 84: 46–55.
Sigler, L., Harris, J. and Dixon, D. 1990. Microbiology and potential virulence of Sporothrix cyanescens, a fungus rarely isolated from blood and skin. Journal of Clinical Microbiology 29: 1009–1115.
Stodůlková, E., Císařová, I., Kolařík, M., Chudíčková, M., Novák, P., et al. 2015. Biologically active metabolites produced by the basidiomycete Quambalaria cyanescens. PLoS One 10: p.e0118913.
Simpson, J.A. 2000. Quambalaria, a new genus of eucalypt pathogens. Australasian Mycologist 19: 57–62.
Stupar, M., Savković, Ž., Breka, K., Stamenković, S., Krizmanić, I., et al. 2023. A variety of fungal species on the green frogs’ skin (Pelophylax esculentus complex) in South Banat. Microbial Ecology 86: 859–871.
Vahedi-Darmiyan, M.E., Jahani, M., Mirzaee, M.R. and Asgari, B. 2017. A noteworthy record of endophytic Quambalaria cyanescens from Punica granatum in Iran. Czech Mycology 69: 113–123.
White, T.J., Bruns, T., Lee, S.J.W.T. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols. A Guide to Methods and Applications, 18.1: 315–322 | ||
|
آمار تعداد مشاهده مقاله: 331 تعداد دریافت فایل اصل مقاله: 527 |
||