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Prevalence of biofilm and efflux pump genes expression by PCR and antibiotic resistance pattern in Pseudomonas aeruginosa | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 01 اردیبهشت 1403 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2024.365159.3045 | ||
| نویسندگان | ||
| Arash Soltani Borchaloee* 1؛ Leila Sadat Moosakazemi Mohammadi2؛ Roya Khosh Ravesh3؛ Seyede Fatemeh Allameh4؛ Fatemeh Sadat Tabatabaie Poya5؛ Ayda Fatehi Marj6 | ||
| 1SabaBiomedicals Science-Based Company, Tehran, Iran | ||
| 2Department of Biology, Faculty of Basic Science, Semnan University, Semnan, Iran | ||
| 3Department of physiology, school of Medicine Iran University of Medical Science, Tehran, Iran | ||
| 4Department of Biology, Faculty of Sciences, Islamic Azad University of Shiraz,Shiraz,Iran | ||
| 5Department of Biology, College of Basic Sciences, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran | ||
| 6Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| چکیده | ||
| Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections. P. aeruginosa is a multidrug-resistant (MDR) bacteria that is thought to be a major cause of nosocomial infections because of its plasmid-borne and intrinsic resistance to several drugs. This study looked at the possibility of biofilm formation, the distribution of the pslD, pelF, and algD genes, as well as the expression of the MexAB-OprM efflux pump genes. It also looked at the pattern of antibiotic resistance in multi-drug resistant P. aeruginosa isolates that were taken from various clinical samples. 76 P. aeruginosa strains had been collected for this investigation from various clinical specimens. Using the disk agar diffusion method, the isolates' susceptibility to antibiotics was assessed. Lastly, multi-drug resistance (MDR) is based on the resistance pattern. These isolates were assessed for the presence of three key biofilm genes and antimicrobial resistance patterns against ten standard antibiotic disks. Version 25 of the SPSS statistical software was used to analyze the data. Examination of the isolates showed that the most antibiotic sensitivity is related to polymyxin, piperacillin, and ciprofloxacin. Also, the prevalence of biofilm-producing genes pslD, pelF, and algD genes was assessed as 68.4%, 80.3%, and 69.7% respectively. The prevalence of MexAB-OprM efflux genes in the examined isolates, mexA, mexB, and oprM genes were 89.5%, 90.8%, and 90.8%, respectively. The majority of the isolates in this investigation had efflux pump genes, according to the findings. Additionally, a strong correlation was discovered between a few efflux genes and biofilm, or the antibiotics tetracycline, meropenem, amikacin, and polymyxin B. | ||
| کلیدواژه‌ها | ||
| Pseudomonas aeruginosa؛ efflux pump؛ biofilm؛ MDR | ||
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آمار تعداد مشاهده مقاله: 93 |
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