اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,909
تعداد مقالات32,812
تعداد مشاهده مقاله43,136,230
تعداد دریافت فایل اصل مقاله19,709,557
Najafi, Mahdiyeh, Khordadmehr, Monireh, baradaran, Behzad, Najafi, Souzan, Amini, Mohammad, Asadpour, Reza. (1403). The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro. سامانه مدیریت نشریات علمی, 79(6), 1249-1256. doi: 10.32592/ARI.2024.79.6.1249
Mahdiyeh Najafi; Monireh Khordadmehr; Behzad baradaran; Souzan Najafi; Mohammad Amini; Reza Asadpour. "The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro". سامانه مدیریت نشریات علمی, 79, 6, 1403, 1249-1256. doi: 10.32592/ARI.2024.79.6.1249
Najafi, Mahdiyeh, Khordadmehr, Monireh, baradaran, Behzad, Najafi, Souzan, Amini, Mohammad, Asadpour, Reza. (1403). 'The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro', سامانه مدیریت نشریات علمی, 79(6), pp. 1249-1256. doi: 10.32592/ARI.2024.79.6.1249
Najafi, Mahdiyeh, Khordadmehr, Monireh, baradaran, Behzad, Najafi, Souzan, Amini, Mohammad, Asadpour, Reza. The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro. سامانه مدیریت نشریات علمی, 1403; 79(6): 1249-1256. doi: 10.32592/ARI.2024.79.6.1249

The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro

Archives of Razi Institute
مقاله 15، دوره 79، شماره 6، بهمن و اسفند 2024، صفحه 1249-1256    اصل مقاله (428.89 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.6.1249
نویسندگان
Mahdiyeh Najafi1؛ Monireh Khordadmehr* 1؛ Behzad baradaran2؛ Souzan Najafi3؛ Mohammad Amini3؛ Reza Asadpour4
1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, 51665-1647, Tabriz, Iran
2Immunology Research Center, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran, and Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran
3Immunology Research Center, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran
4Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, 51665-1647, Tabriz, Iran
چکیده
Breast cancer is the most commonly diagnosed cancer among women worldwide. In recent years, there has been growing interest among researchers in exploring alternative therapeutic methods, including stem cell therapy. Therefore, this study aimed to investigate the effects of adipose-derived mesenchymal stem cell-conditioned media (AD-MSCs-CM) on apoptosis induction and migration inhibition of breast cancer cells (MDA-MB-231) in vitro. Here, malignant breast cancer cells (MDA-MB-231) and adipose mesenchymal stem cells (AD-MSC) were cultured separately in DMEM F12/FBS15% culture media in standard conditions. Subsequently, the conditioned media derived from AD-MSCs was exposed to the MDA-MB-231 cells. After 24 and 48 hours of exposure, the expression levels of CASP3, KRAS, and MMP9, were assessed using a qRT-PCR assay. Additionally, the proliferation and migration abilities of the cancer cells were evaluated using MTT and wound healing assays, respectively. Also, the protein expression of Caspase-3, K-RAS, and MMP-9 was examined using the western blot assay. Notably, the expression level of MMP9 and KRAS genes significantly decreased following AD-MSCs-CM treatment in MDA-MB-231 cells. Moreover, the expression level of CASP3 gene remarkably increased in the treated groups. Besides, the proliferation of MDA-MB-231 cells treated with AD-MSCs-CM was strongly reduced by MTT and wound healing assays. Furthermore, the AD-MSCs-CM demonstrated an increase in the activation of Caspase-3 and a decrease in the protein expressions of K-RAS and MMP-9. The findings of this study suggest that the AD-MSCs-CM have the potential to influence apoptosis, proliferation, and migration of breast cancer cells. Thus, it could be considered a promising therapeutic strategy for suppressing breast cancer. However, further testing and research are required to validate these findings and explore the full potential of this approach.
کلیدواژه‌ها
Breast cancer؛ Stem cells؛ Apoptosis؛ Migration؛ Gene expression
عنوان مقاله [English]
بررسی اثر محیط کشت سلول های بنیادی مزانشیمی بر رفتار بدخیمی سلول های سرطان پستان در شرایط آزمایشگاهی
چکیده [English]
سرطان پستان شایع ترین سرطان تشخیص داده شده در بین زنان در سراسر جهان است. در سال‌های اخیر، علاقه فزاینده‌ای در میان محققان به بررسی روش‌های درمانی جایگزین، از جمله درمان با سلول‌های بنیادی، وجود داشته است. بنابراین، این مطالعه با هدف بررسی اثرات محیط کشت سلولهای بنیادی بر سلول های بنیادی مزانشیمی مشتق از چربی (AD-MSCs-CM) بر القای آپوپتوز و مهار مهاجرت سلول های سرطان پستان (MDA-MB-231) در شرایط آزمایشگاهی انجام شد. در این مطالعه، سلول های سرطانی بدخیم پستان (MDA-MB-231) و سلول های بنیادی مزانشیمی چربی (AD-MSC) به طور جداگانه در محیط های کشت DMEM F12/FBS15% در شرایط استاندارد کشت داده شدند. متعاقبا، محیط شرطی شده مشتق شده از سلول های بنیادی مزانشیمی AD-MSC در معرض سلول های MDA-MB-231 قرار گرفت. پس از 24 و 48 ساعت قرار گرفتن در معرض، سطح بیان CASP3، KRAS و MMP9 با استفاده از روش qRT-PCR ارزیابی شد. علاوه بر این، توانایی‌های تکثیر و مهاجرت سلول‌های سرطانی به ترتیب با استفاده از روش‌های MTT و تست خراش مورد ارزیابی قرار گرفت. همچنین بیان پروتئین کاسپاز-3، K-RAS و MMP-9 با استفاده از روش وسترن بلات مورد بررسی قرار گرفت. قابل ذکر است که سطح بیان ژن های MMP9 و KRAS به دنبال درمان AD-MSCs-CM در سلول های MDA-MB-231 به طور قابل توجهی کاهش یافت. همچنین میزان بیان ژن CASP3 در گروه‌های آزمایش به‌طور قابل‌توجهی افزایش یافت. علاوه بر این، تکثیر سلول‌های MDA-MB-231 تحت درمان با AD-MSCs-CM توسط روش‌های MTT و تست خراش به شدت کاهش یافت. علاوه بر این، AD-MSCs-CM افزایش در فعال سازی کاسپاز-3 و کاهش بیان پروتئین K-RAS و MMP-9 را نشان داد. یافته‌های این مطالعه نشان می‌دهد که AD-MSCs-CM پتانسیل تأثیرگذاری بر آپوپتوز، تکثیر و مهاجرت سلول‌های سرطان پستان را دارد. بنابراین، می توان آن را یک استراتژی درمانی امیدوارکننده برای سرکوب سرطان پستان در نظر گرفت. با این حال، برای تأیید این یافته‌ها و کشف پتانسیل کامل این رویکرد، آزمایش‌ها و تحقیقات بیشتری لازم است.
کلیدواژه‌ها [English]
سرطان پستان, سلول بنیادی, آپپتوز, مهاجرت, بیان ژن
مراجع
  1. Nolan E, Lindeman GJ, Visvader JE. Deciphering breast cancer: from biology to the clinic. Cell. 2023;186(8):1708-28.
  2. Zagami P, Carey LA. Triple negative breast cancer: Pitfalls and progress. NPJ breast cancer. 2022;8(1):95.
  3. Li Y, Zhang H, Merkher Y, Chen L, Liu N, Leonov S, et al. Recent advances in therapeutic strategies for triple-negative breast cancer. Journal of hematology & oncology. 2022;15(1):121.
  4. Jin MC, Medress ZA, Azad TD, Doulames VM, Veeravagu A. Stem cell therapies for acute spinal cord injury in humans: a review. Neurosurgical focus. 2019;46(3):E10.
  5. Dong L-H, Jiang Y-Y, Liu Y-J, Cui S, Xia C-C, Qu C, et al. The anti-fibrotic effects of mesenchymal stem cells on irradiated lungs via stimulating endogenous secretion of HGF and PGE2. Scientific Reports. 2015;5(1):8713.
  6. Tu Z, Karnoub AE. Mesenchymal stem/stromal cells in breast cancer development and management. Seminars in cancer biology. 2022;86(Pt 2):81-92.
  7. Liu J, Gao J, Liang Z, Gao C, Niu Q, Wu F, et al. Mesenchymal stem cells and their microenvironment. Stem cell research & therapy. 2022;13(1):429.
  8. Lin Z, Wu Y, Xu Y, Li G, Li Z, Liu T. Mesenchymal stem cell-derived exosomes in cancer therapy resistance: recent advances and therapeutic potential. Molecular cancer. 2022;21(1):179.
  9. Ra K, Park SC, Lee BC. Female Reproductive Aging and Oxidative Stress: Mesenchymal Stem Cell Conditioned Medium as a Promising Antioxidant. International journal of molecular sciences. 2023;24(5).
  10. Alió Del Barrio JL, De la Mata A, De Miguel MP, Arnalich-Montiel F, Nieto-Miguel T, El Zarif M, et al. Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells. Cells. 2022;11(16).
  11. Chakraborty S, Rahman T. The difficulties in cancer treatment. Ecancermedicalscience. 2012;6:ed16.
  12. Xuan X, Tian C, Zhao M, Sun Y, Huang C. Mesenchymal stem cells in cancer progression and anticancer therapeutic resistance. Cancer Cell International. 2021;21(1):595.
  13. Al-Ejeh F, Smart CE, Morrison BJ, Chenevix-Trench G, López JA, Lakhani SR, et al. Breast cancer stem cells: treatment resistance and therapeutic opportunities. Carcinogenesis. 2011;32(5):650-8.
  14. He N, Kong Y, Lei X, Liu Y, Wang J, Xu C, et al. MSCs inhibit tumor progression and enhance radiosensitivity of breast cancer cells by down-regulating Stat3 signaling pathway. Cell Death Dis. 2018;9(10):1026-.
  15. Kim J, Hall RR, Lesniak MS, Ahmed AU. Stem Cell-Based Cell Carrier for Targeted Oncolytic Virotherapy: Translational Opportunity and Open Questions. Viruses. 2015;7(12):6200-17.
  16. Cuiffo BG, Karnoub AE. Mesenchymal stem cells in tumor development: emerging roles and concepts. Cell adhesion & migration. 2012;6(3):220-30.
  17. Hmadcha A, Martin-Montalvo A, Gauthier BR, Soria B, Capilla-Gonzalez V. Therapeutic Potential of Mesenchymal Stem Cells for Cancer Therapy. Frontiers in bioengineering and biotechnology. 2020;8:43.
  18. Huo Q, Li K, Sun X, Zhuang A, Minami K, Tamari K, et al. The inhibition of pancreatic cancer progression by K-Ras-overexpressing mesenchymal stem cell-derived secretomes. Scientific Reports. 2023;13(1):15036.
  19. Jahangiri B, Khalaj-Kondori M, Asadollahi E, Purrafee Dizaj L, Sadeghizadeh M. MSC-Derived exosomes suppress colorectal cancer cell proliferation and metastasis via miR-100/mTOR/miR-143 pathway. International journal of pharmaceutics. 2022;627:122214.
  20. Khalil C, Moussa M, Azar A, Tawk J, Habbouche J, Salameh R, et al. Anti-proliferative effects of mesenchymal stem cells (MSCs) derived from multiple sources on ovarian cancer cell lines: an in-vitro experimental study. Journal of ovarian research. 2019;12(1):70.
  21. Zhao J, Zhang Z, Cui Q, Zhao L, Hu Y, Zhao S. Human adipose-derived mesenchymal stem cells inhibit proliferation and induce apoptosis of human gastric cancer HGC-27 cells. 3 Biotech. 2020;10(3):129.
  22. Gentile P. Breast Cancer Therapy: The Potential Role of Mesenchymal Stem Cells in Translational Biomedical Research. Biomedicines. 2022;10(5):1179.
  23. Trivanović D, Nikolić S, Krstić J, Jauković A, Mojsilović S, Ilić V, et al. Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. Cell biology international. 2014;38(2):254-65.
  24. Ahn JO, Coh YR, Lee HW, Shin IS, Kang SK, Youn HY. Human adipose tissue-derived mesenchymal stem cells inhibit melanoma growth in vitro and in vivo. Anticancer research. 2015;35(1):159-68.
  25. Ryu H, Oh JE, Rhee KJ, Baik SK, Kim J, Kang SJ, et al. Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and suppress the growth of MCF-7 human breast cancer cells. Cancer letters. 2014;352(2):220-7.
  26. Kalamegam G, Sait KHW, Anfinan N, Kadam R, Ahmed F, Rasool M, et al. Cytokines secreted by human Wharton's jelly stem cells inhibit the proliferation of ovarian cancer (OVCAR3) cells in vitro. Oncology letters. 2019;17(5):4521-31.
  27. Yang C, Lei D, Ouyang W, Ren J, Li H, Hu J, et al. Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro. BioMed research international. 2014;2014:109389.
  28. Li L, Tian H, Chen Z, Yue W, Li S, Li W. Inhibition of lung cancer cell proliferation mediated by human mesenchymal stem cells. Acta biochimica et biophysica Sinica. 2011;43(2):143-8.
  29. Li P, Zhou H, Di G, Liu J, Liu Y, Wang Z, et al. Mesenchymal stem cell-conditioned medium promotes MDA-MB-231 cell migration and inhibits A549 cell migration by regulating insulin receptor and human epidermal growth factor receptor 3 phosphorylation. Oncology letters. 2017;13(3):1581-6.
  30. Xie H, Liao N, Lan F, Cai Z, Liu X, Liu J. 3D-cultured adipose tissue-derived stem cells inhibit liver cancer cell migration and invasion through suppressing epithelial-mesenchymal transition. International journal of molecular medicine. 2018;41(3):1385-96.
  31. Yousef EM, Tahir MR, St-Pierre Y, Gaboury LA. MMP-9 expression varies according to molecular subtypes of breast cancer. BMC cancer. 2014;14:609.
  32. Eckert LB, Repasky GA, Ulkü AS, McFall A, Zhou H, Sartor CI, et al. Involvement of Ras activation in human breast cancer cell signaling, invasion, and anoikis. Cancer research. 2004;64(13):4585-92.
  33. Uprety D, Adjei AA. KRAS: From undruggable to a druggable Cancer Target. Cancer treatment reviews. 2020;89:102070.
  34. Van Sciver RE, Njogu MM, Isbell AJ, Odanga JJ, Bian M, Svyatova E, et al. Chapter 12 - Blocking SIAH Proteolysis, an Important K-RAS Vulnerability, to Control and Eradicate K-RAS-Driven Metastatic Cancer. In: Azmi AS, editor. Conquering RAS. Boston: Academic Press; 2017. p. 213-32.
  35. Gupta GK, Collier AL, Lee D, Hoefer RA, Zheleva V, Siewertsz van Reesema LL, et al. Perspectives on Triple-Negative Breast Cancer: Current Treatment Strategies, Unmet Needs, and Potential Targets for Future Therapies. Cancers. 2020;12(9).
آمار
تعداد مشاهده مقاله: 183
تعداد دریافت فایل اصل مقاله: 203


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب