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Production, characterization, and evaluation of polyclonal antisera specific to stellate sturgeon (Acipenser stellatus) immunoglobulin | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 23، شماره 3، مرداد 2024، صفحه 427-435 اصل مقاله (338.39 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2024.131141 | ||
| نویسندگان | ||
| A. Yektaseresht* 1؛ Z. Hemati2؛ A. Gholamhosseini3؛ AA. Khorsand4 | ||
| 11Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran | ||
| 3Department of Clinical Sciences, Department, Veterinary School, Shiraz University Shiraz, Iran | ||
| 4Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Sturgeon species are commercially important species in finfish aquaculture industry. Till now, a small number of studies have been reported on the immune responses in sturgeon species against infectious diseases. Monoclonal and polyclonal antibodies in fish immunoglobulins (Igs) are essential to evaluate the humoral immune response. This study aimed to produce, characterize, and evaluate the polyclonal antisera against stellate sturgeon Igs. For the production of polyclonal antibodies against fish Igs, stellate sturgeon serum Igs was isolated using the ammonium sulfate precipitation method. The purified Igs with the Freund adjuvant was injected subcutaneously in to sheep (Ovis aries). Then, anti- stellate sturgeon Ig antibody titer and specificity were determined using ELISA and Western blot techniques. Stellate sturgeon Ig antibodies were detected in the immunized animals and were significantly higher in the immunized group compared to the non-immunized group. The polyclonal antisera showed a high specificity to the immunoglobulin heavy )H( chain of stellate sturgeon and moderately cross-reacted with the immunoglobulin H chain in Siberian (Acipenser baerii) and ship (A. nudiventris) sturgeons but did not show reactivity with rainbow trout (Oncorhynchus mykiss) serum Igs. It was indicated that the produced polyclonal antisera should be further investigated to be used for immune-diagnostic purposes in sturgeon species upon exposure to various infectious diseases. | ||
| کلیدواژهها | ||
| Acipenser stellatus؛ Immunoglobulin؛ Polyclonal antibody؛ Sturgeon | ||
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