- همه نشریات
- نشریات علمی سازمان تحقیقات، آموزش و ترویچ کشاورزی
- مجلات ترویجی سازمان تحقیقات، آموزش و ترویج کشاورزی
- سایر نشریات سازمان تحقیقات، آموزش و ترویج کشاورزی
- نشریات علمی انجمن ها
- علوم آب و خاک
- علوم اقتصاد و ترویج کشاورزی
- علوم جنگل, مرتع و آبخیزداری
- علوم دامی و شیلات
- علوم زراعی و باغی
- علوم گیاهپزشکی
- علوم مهندسی و مکانیزاسیون کشاورزی
- نشریات با زبان انگلیسی
- نشریات با زبان فارسی
- نشریات دانشگاه آزاد اسلامی
- نشریات علمی
پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 3,000 |
| تعداد مقالات | 33,577 |
| تعداد مشاهده مقاله | 44,314,332 |
| تعداد دریافت فایل اصل مقاله | 23,610,989 |
Investigating biological characteristics of two jellyfish (Rhopilema nomadica and Chrysaora hysoscella) venoms on human fibroblast | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 23، شماره 1، فروردین 2024، صفحه 171-195 اصل مقاله (750.46 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2024.130894 | ||
| نویسندگان | ||
| S. Ketabdari1؛ H. Zolgharnein1؛ H.R. Goudarzi* 2؛ A. Savari1؛ Z. Salehi Najaf Abadi2 | ||
| 1Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr Marine Science and Technology University, Khorramshahr, Iran | ||
| 2Research and Development Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| This paper aims to investigate the biological and cytotoxic characteristics of venoms extracted from Rhopilema nomadica and Chrysaora hysoscella jellyfish collected from the Persian Gulf. The cytotoxic effect of the venoms is surveyed on human fibroblast skin cells (HU02) using the The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Moreover, the protein molecular weight was determined and the toxicity test (LD50) of venoms were explored on BALB/c mice. The R. nomadica venom possessed a higher protein concentration with the lowest molecular weight protein via SDS-PAGE (12.5 %) along with more peaks obtained by HPLC. In addition, the results of both LD50 and MTT are affected by the venom characteristics. Besides, intravenously and intraperitoneally LD50 were 1.26 and 2.35 for C. hysesella and 0.65 IV and 1.6 IP for R. nomadica, respectively, suggesting that R. nomadica venom was more lethal. The results of the MTT assay on Hu02 fibroblast cells for 24 h revealed that R. nomadica had more lethal effects on skin cells compared to C. hysoscella. Escherichia coli, whereas methicillin-resistant Staphylococcus aureus had no antibacterial activities in the presence of both R. nomadica and C. hysoscella venoms. Meanwhile, the venoms had antibacterial effects on Pseudomonas aeruginosa and S. aureus, which were still weak compared to the other reported species. Overall, R. nomadica venom was more lethal in the case of mice and human skin cells and likely with more symptoms in prey. Finally, considering the relatively high viability of different bacteria in diverse dilutions of venom, it seems that victims not only should face dermal injuries and possible scars caused by direct stings but also the presence of venomous protein on the dermal tissue may provide a cultivation medium for different kind of bacteria. | ||
| کلیدواژه‌ها | ||
| Bacterial viability rate؛ Bradford assay؛ Human fibroblast skin cells [HU02]؛ Jellyfish venom؛ MTT assay | ||
|
آمار تعداد مشاهده مقاله: 352 تعداد دریافت فایل اصل مقاله: 335 |
||
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب