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Karimian, Paridokht, Mojtahedi, Ali, Anvari, Masoumeh, Mesbahi, G, Ghalandari, Maryam. (1403). The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran. سامانه مدیریت نشریات علمی, 79(5), 955-961. doi: 10.32592/ARI.2024.79.5.955
Paridokht Karimian; Ali Mojtahedi; Masoumeh Anvari; G Mesbahi; Maryam Ghalandari. "The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran". سامانه مدیریت نشریات علمی, 79, 5, 1403, 955-961. doi: 10.32592/ARI.2024.79.5.955
Karimian, Paridokht, Mojtahedi, Ali, Anvari, Masoumeh, Mesbahi, G, Ghalandari, Maryam. (1403). 'The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran', سامانه مدیریت نشریات علمی, 79(5), pp. 955-961. doi: 10.32592/ARI.2024.79.5.955
Karimian, Paridokht, Mojtahedi, Ali, Anvari, Masoumeh, Mesbahi, G, Ghalandari, Maryam. The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran. سامانه مدیریت نشریات علمی, 1403; 79(5): 955-961. doi: 10.32592/ARI.2024.79.5.955

The First Report of t8463 and t605 spa Types in Methicillin-resistant Staphylococcus aureus Isolated from ICUs in Rasht, Iran

Archives of Razi Institute
مقاله 10، دوره 79، شماره 5، آذر و دی 2024، صفحه 955-961    اصل مقاله (332.32 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.5.955
نویسندگان
Paridokht Karimian1؛ Ali Mojtahedi* 2؛ Masoumeh Anvari3؛ G Mesbahi1؛ Maryam Ghalandari4
1Guilan University of Medical Sciences
2Iran University of Medical Sciences
3Islamic Azad University
4Shahid Sadoughi University of Medical Sciences and Health Services
چکیده
Staphylococcus aureus is the most important human pathogen, in community and hospital-acquired infections. The colonization rate of this organism is high in medical personnel and devices. Considering the importance of carriers in the transmission of S. aureus infection, this study investigated the origin of methicillin-resistant S. aureus (MRSA) isolated from Velayat and Poursina Hospitals of Rasht. In eight months, 500 samples were obtained from hospitalized patients, personnel, different surfaces, air, and devices in the intensive care unit (ICU) departments of Velayat and Poursina Hospitals. After the identification of MRSA strains by microbiological and biochemical standard methods, the DNA of the isolates was extracted. The spa typing of MRSA strains was done after determining the sequence of amplified protein A genes by polymerase chain reaction (PCR) using specific primers. Among 500 samples, 45 (9%) samples were infected with S. aureus and 31 (68.9%) MRSA strains were identified from different ICUs. For the first time, S. aureus was divided into three types with the help of the spa technique in the ICU of Rasht hospitals. Among the examined samples, the t14870 spa type prevailed (95.5%), which had been found in previous studies in different regions of Iran. However, two types, t8463 (2.2%) and t605 (2.2%), were obtained for the first time in Iran, which were MRSA and obtained from the noses of patients. High frequency of S. aureus isolates indicated in ICUs and personnel play a very effective role in the transmission of infection in the hospital, so need for infection control in the hospital is inevitable.
کلیدواژه‌ها
Methicillin resistant S. aureus (MRSA)؛ ICU؛ Staphylococcus protein A؛ spa typing
عنوان مقاله [English]
اولین گزارش از تیپ های spa t8463 و spa t605 در استافیلوکوکوس اورئوس مقاوم به متی سیلین جدا شده از بخش های مراقبت ویژه رشت، ایران
چکیده [English]
استافیلوکوکوس اورئوس مهم ترین پاتوژن انسانی در عفونت های اکتسابی از جامعه و بیمارستان است. میزان کلونیزاسیون این ارگانیسم در پرسنل پزشکی زیاد است. با توجه به اهمیت ناقلین در انتقال این عفونت، این مطالعه به بررسی منشا استافیلوکوکوس اورئوس مقاوم به متی سیلین (MRSA) جدا شده از بیمارستان های ولایت و پورسینای رشت پرداخت. در مدت هشت ماه، 500 نمونه از بیماران بستری، پرسنل، سطوح مختلف، هوا و دستگاه های بخش های آی سی یو بیمارستان های ولایت و پورسینا تهیه شد. پس از شناسایی سویه‌های MRSA با روش‌های استاندارد میکروبیولوژیکی و بیوشیمیایی، DNA جدایه‌ها استخراج شد. تیپ بندی spa سویه‌های MRSA پس از تعیین توالی ژن‌های پروتئین A تکثیر شده توسط PCR با استفاده از پرایمرهای اختصاصی انجام شد. از بین 500 نمونه، 45 (9%) نمونه آلوده به استافیلوکوکوس اورئوس و 31 (68.9%) سویه MRSA از واحدهای مراقبت‌های ویژه شناسایی شدند. استافیلوکوکوس اورئوس برای اولین بار با استفاده از تکنیک spa در بخش مراقبت های ویژه بیمارستان های رشت به سه تیپ تقسیم شد. در بین نمونه های مورد بررسی، تیپ spa t14870 (95.5 درصد) غالب بود که در مطالعات قبلی در مناطق مختلف ایران یافت شده بود. اما دو نوع t8463 (2.2%) و t605 (2.2%) برای اولین بار در ایران بدست آمد که MRSA بود و از بینی بیماران به دست آمد. فراوانی بالای ایزوله های استافیلوکوکوس اورئوس نشان داده شده در بخش های مراقبت ویژه و پرسنل نقش بسیار موثری در انتقال عفونت در بیمارستان دارد، بنابراین نیاز به کنترل عفونت در بیمارستان یک موضوع اجتناب ناپذیر است.
کلیدواژه‌ها [English]
استافیلوکوکوس اورئوس مقاوم به متی سیلین (MRSA), ICU, پروتئین A استافیلوکوکوس, تیپ بندی spa
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