اخبار و اعلانات

 آمار

تعداد نشریات285
تعداد نشریات سازمان83
تعداد شماره‌ها2,826
تعداد مقالات32,014
تعداد مشاهده مقاله40,731,203
تعداد دریافت فایل اصل مقاله18,016,587
Hemmati, Zahra, Soltani Borchaloee, Arash, Bazrafshan, Fateme, Jahan Latibari, Bahar, Mehrpour Ghaziani, Paniz, Hashemi Khou, Maryam. (1403). Study on the prevalence of methicillin-resistant Staphylococcus aureus infection, antibiotic resistance pattern, biofilms genes, and antibiotic resistance genes from clinical samples. سامانه مدیریت نشریات علمی, 79(5), 923-928. doi: 10.32592/ARI.2024.79.5.923
Zahra Hemmati; Arash Soltani Borchaloee; Fateme Bazrafshan; Bahar Jahan Latibari; Paniz Mehrpour Ghaziani; Maryam Hashemi Khou. "Study on the prevalence of methicillin-resistant Staphylococcus aureus infection, antibiotic resistance pattern, biofilms genes, and antibiotic resistance genes from clinical samples". سامانه مدیریت نشریات علمی, 79, 5, 1403, 923-928. doi: 10.32592/ARI.2024.79.5.923
Hemmati, Zahra, Soltani Borchaloee, Arash, Bazrafshan, Fateme, Jahan Latibari, Bahar, Mehrpour Ghaziani, Paniz, Hashemi Khou, Maryam. (1403). 'Study on the prevalence of methicillin-resistant Staphylococcus aureus infection, antibiotic resistance pattern, biofilms genes, and antibiotic resistance genes from clinical samples', سامانه مدیریت نشریات علمی, 79(5), pp. 923-928. doi: 10.32592/ARI.2024.79.5.923
Hemmati, Zahra, Soltani Borchaloee, Arash, Bazrafshan, Fateme, Jahan Latibari, Bahar, Mehrpour Ghaziani, Paniz, Hashemi Khou, Maryam. Study on the prevalence of methicillin-resistant Staphylococcus aureus infection, antibiotic resistance pattern, biofilms genes, and antibiotic resistance genes from clinical samples. سامانه مدیریت نشریات علمی, 1403; 79(5): 923-928. doi: 10.32592/ARI.2024.79.5.923

Study on the prevalence of methicillin-resistant Staphylococcus aureus infection, antibiotic resistance pattern, biofilms genes, and antibiotic resistance genes from clinical samples

Archives of Razi Institute
دوره 79، شماره 5، آذر و دی 2024، صفحه 923-928    اصل مقاله (524.89 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.5.923
نویسندگان
Zahra Hemmati1؛ Arash Soltani Borchaloee* 2؛ Fateme Bazrafshan3؛ Bahar Jahan Latibari1؛ Paniz Mehrpour Ghaziani4؛ Maryam Hashemi Khou5
1Department of Biology, Faculty of Basic Science, Semnan University, Semnan, Iran
2SabaBiomedicals Science-Based Company, Tehran, Iran
3Department of Biology, Faculty of Sciences, Yazd University, Yazd, Iran
4Department of Biology, Faculty of Basic Science, Mohaghegh Ardabili University, Ardabil, Iran
5Department of microbiology, neyshabour Branch, Islamic Azad University Shiraz, Iran
چکیده
The global health situation is caused by Methicillin-Resistant Staphylococcus aureus (MRSA) strains because they are resistant to most antibiotics. The emergence and spread of antibiotic resistance make the treatment of these infections more complicated. This study aimed to investigate mecA, blaZ, cna, and fnbA genes and the pattern of antibiotic resistance in S. aureus isolates obtained from different clinical samples. In this study, 78 strains of S. aureus were collected from different clinical specimens. The antibiotic susceptibility of the isolates was determined by the disk agar diffusion method. The prevalence of mecA, blaZ, cna, and fnbA genes and antimicrobial resistance patterns against 10 conventional antibiotic disks were evaluated in these isolates. Data analysis was performed utilizing SPSS statistical software version 25. Of the 78 collected samples, 63 samples had the mecA gene (62.2%). 63 isolates of S. aureus were examined, in which the blaZ gene is present in 60 (95.2%) isolates, and the fnbA gene is present in 51 (81%) isolates. The frequency of the cna gene was observed in 42 (66.7%). also, a significant relationship was observed between cna and fnbA genes with gentamicin and tetracycline antibiotic resistance with (P<0.05). The results of the antibiotic resistance pattern showed that all the isolates were resistant to oxacillin (100%), penicillin (95.2%), and the least resistant was vancomycin (3.2%), and Trimethoprim-sulfamethoxazole (17.5%).
Compared with other reports from Iran, our study shows an average rate of MRSA. However, the level of resistance to common antibiotics in these isolates was high. In this situation, it is recommended to monitor antibiotic resistance in these hospitals and medical centers.
کلیدواژه‌ها
Staphylococcus aureus؛ MRSA؛ Antibiotic resistance؛ Biofilm
مراجع
  1. Gaddafi MS, Yakubu Y, Junaidu AU, Bello MB, Bitrus AA, Musawa AI, Garba B, Lawal H. Occurrence of Methicillin-resistant Staphylococcus aureus (MRSA) From Dairy Cows in Kebbi, Nigeria. Iranian Journal of Veterinary Medicine. 2023;17(1): 19-26.
  2. Foroutan S, Eslampour MA, Emaneini M, Jabalameli F, Akbari G. Characterization of Biofilm Formation Ability, Virulence Factors and Antibiotic Resistance Pattern of Staphylococcus aureus Isolates from Subclinical Bovine Mastitis. Iranian Journal of Veterinary Medicine. 2022;16(2):144-154.
  3. Azizkhani M, Tooryan F. Methicillin resistant Staphylococcus aureus (MRSA) in pastry cream products sold in Amol (Iran). Iranian Journal of Veterinary Medicine. 2018;12(2): 167-174.
  4. Azizkhani M, Akhondzadeh Basti A, Tooryan F. Comparing inhibitory potential of Eugenia caryophyllus and Origanum compactum against the growth and gene expression of enterotoxins in Staphylococcus aureus ATCC 29213. Iranian Journal of Veterinary Medicine. 2017; 11(4): 299-311.
  5. Azizkhani M, Jafari F, Haghighi P, Dehghan M. Evaluating Contamination Level of Raw and Roasted Nuts Distributed in Commercial Markets in Mazandaran Province, Iran. Iranian Journal of Veterinary Medicine. 2020;14(2):167-176.
  6. Takayama Y, Tanaka T, Oikawa K, Fukano N, Goto M, Takahashi T. Prevalence of blaZ gene and performance of phenotypic tests to detect penicillinase in Staphylococcus aureus isolates from Japan. Annals of laboratory medicine. 2018; 28;38(2):155-59.
  7. Olsen JE, Christensen H, Aarestrup FM. Diversity and evolution of blaZ from Staphylococcus aureus and coagulase-negative staphylococci. Journal of Antimicrobial Chemotherapy. 2006; 1;57(3):450-60.
  8. Foster TJ. Immune evasion by staphylococci. Nature reviews microbiology. 2005;3(12):948-58.
  9. Kot B, Sytykiewicz H, Sprawka I. Expression of the biofilm-associated genes in methicillin-resistant Staphylococcus aureus in biofilm and planktonic conditions. International journal of molecular sciences. 2018;19(11):3487.
  10. Kadkhoda H, Ghalavand Z, Nikmanesh B, Kodori M, Houri H, Maleki DT, et al. Characterization of biofilm formation and virulence factors of Staphylococcus aureus isolates from paediatric patients in Tehran, Iran. Iranian Journal of Basic Medical Sciences. 2020;23(5):691.
  11. Foster TJ, Höök M. Surface protein adhesins of Staphylococcus aureus. Trends in microbiology. 1998;6(12):484-488.
  12. Shahmoradi M, Faridifar P, Shapouri R, Mousavi SF, Ezzedin M, Mirzaei B. Determining the biofilm forming gene profile of Staphylococcus aureus clinical isolates via multiplex colony PCR method. Reports of biochemistry & molecular biology. 2019;7(2):181.
  13. Abdulrahman RF. DETECTION OF Staphylococcus aureus FROM LOCAL AND IMPORTED chicken in duhok province/kurdistan region of iraq using conventional and molecular methods. Basrah Journal of Veterinary Research. 2020;19(1).
  14. Performance Standards for Antimicrobial Susceptibility Testing CLSI Supplement M100. 32th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2022.
  •  
  1. Rahimi F, Khashei S, Katouli M. Prevalence, diversity, and antimicrobial susceptibility proles of methicillin-resistant Staphylococcus aureus among patients with diabetic foot infections in a referral hospital in Tehran, Iran. Research Square. 2023;19(3):e142081.
  2. Fatholahzadeh B, Emaneini M, Gilbert G, Udo E, Aligholi M, Modarressi MH, Nouri K, Sedaghat H, Feizabadi MM. Staphylococcal cassette chromosome mec (SCC mec) analysis and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) isolates in Tehran, Iran. Microbial drug resistance. 2008;14(3):217-20.
  3. Rahimi H, Saei HD, Ahmadi M. Nasal carriage of Staphylococcus aureus: Frequency and antibiotic resistance in healthy ruminants. Jundishapur journal of microbiology. 2015;8(10):e22413.
  4. Campoccia D, Speziale P, Ravaioli S, Cangini I, Rindi S, Pirini V, Montanaro L, Arciola CR. The presence of both bone sialoprotein-binding protein gene and collagen adhesin gene as a typical virulence trait of the major epidemic cluster in isolates from orthopedic implant infections. Biomaterials. 2009; 30(34): 6621-6628.
  5. Atchade E, De Tymowski C, Grall N, Tanaka S, Montravers P. Toxic Shock Syndrome: A Literature Review. Antibiotics. 2024;13(1):96.
  6. Gomes RMF, Bomfim MRQ, Trindade MJV, Farias LM,Santos SG. Potential Spread of Methicillin-Resistant Staphylococcus aureus Recovered from Patients with Bloodstream Infection. Chemo Open Access. 2015;4(2):149.
  7. Amr GE, Al Gammal S. Emergence of vancomycin resistant Staphylococcus aureus isolated from patients in ICUs of Zagazig University Hospitals. The Egyptian Journal of Medical Microbiology (EJMM). 2017;26(2):1-7.
  8. Kim GY, Lee CH. Antimicrobial susceptibility and pathogenic genes of Staphylococcus aureus isolated from the oral cavity of patients with periodontitis. J Periodontal Implant Sci. 2015;45(6): 223-28.
  9. Naseer BS, Jayaraj YM. Identification of Multi-Resistant Staphylococcus aureus in Clinical Specimens. Res J Med Sci. 2010; 4(3): 204-7.
  10. Mohammadi A, Goudarzi M, Dadashi M, Soltani M, Goudarzi H, Hajikhani B. Molecular detection of genes involved in biofilm formation in Staphylococcus aureus strains isolates: evidence from shahid motahari hospital in Tehran. Jundishapur Journal of Microbiology. 2020;13(7):13(7);e102058.
  11. Khasawneh AI, Himsawi N, Abu-Raideh J, Salameh MA, Al-Tamimi M, Mahmoud SAH, Saleh T. Status of biofilm-forming genes among Jordanian nasal carriers of methicillin-sensitive and methicillin-resistant Staphylococcus aureus. Iranian Biomedical Journal. 2020;24(6):386.
آمار
تعداد مشاهده مقاله: 130
تعداد دریافت فایل اصل مقاله: 7


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب