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Analysis of phylogenetic relationships of Potamogeton and Stuckenia (Potamogetonaceae) in Iran based on morphological, anatomical and molecular data | ||
| The Iranian Journal of Botany | ||
| مقاله 2، دوره 29، شماره 2، اسفند 2023، صفحه 106-121 اصل مقاله (704.45 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2024.130476 | ||
| نویسندگان | ||
| Shabnam Abbasi1؛ Saeed Afsharzadeh* 2؛ Mehri Dinarvand3؛ Ludwig Triest4 | ||
| 1Department of Biology Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran | ||
| 2Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran | ||
| 3Forests and Rangelands Research Division, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran | ||
| 4Laboratory of Plant Biology and Nature Management (APNA), Department of Biology, Vrije Universiteit Brussel (VUB), Brussels, Belgium | ||
| چکیده | ||
| Potamogeton and Stuckenia comprise 90 and 7 respectively accepted species worldwide. Because of a wide range of intraspecific morphological variability including extensive phenotypic plasticity and ecological diversity, Potamogeton and Stuckenia are considered notoriously difficult taxa. A total of 100 accessions representing 11 taxa of Potamogeton and three of Stuckenia were collected across Iran. An initial screening based on allele lengths of highly variable cpDNA sequences allowed considering 59 different accessions of one to eight individuals of each species for further morphological, anatomical, and molecular analyses. NrDNA ITS and three plastid regions (rbcL, matK, and trnH-psbA) were employed to reconstruct molecular phylogenies using maximum likelihood and Bayesian inferences. Analyses of nrDNA ITS sequences generated well-resolved tree topology than plastid data. There was some incongruence between nuclear data and concatenated chloroplast marker data (P=0.001). Additional testing of ISSR and SRAP markers for 48 specimens showed higher resolution in species delimitation among linear leaved taxa, though not conclusive. Anatomical features could not separate Iranian species alone and should be used in combination with morphological characters that were highly informative. The results showed that Iranian Potamogeton species contain a rich gene pool due to the specific and diverse geographical conditions of Iran. | ||
| کلیدواژه‌ها | ||
| aquatic plants؛ chloroplast markers؛ hybridization؛ ITS؛ molecular trees | ||
| عنوان مقاله [English] | ||
| تجزیه و تحلیل روابط فیلوژنتیکی Potamogeton و Stuckenia (Potamogetonaceae) در ایران بر اساس داده‌های مورفولوژیکی، تشریحی و مولکولی | ||
| نویسندگان [English] | ||
| شبنم عباسی1؛ سعید افشارزاده2؛ مهری دیناروند3؛ لودویک تریست4 | ||
| 1گروه آموزش زیست‌شناسی، دانشگاه فرهنگیان، صندوق پستی ۸۸۹-۱۴۶۶۵ تهران، ایران | ||
| 2گروه زیست‌شناسی گیاهی و جانوری، دانشکده علوم و فناوری‌های زیستی، دانشگاه اصفهان، اصفهان، ایران | ||
| 3مرکز تحقیقات کشاورزی و منابع‌طبیعی خوزستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران | ||
| 4آزمایشگاه زیست‌شناسی گیاهی و مدیریت طبیعت، دانشکده زیست‌شناسی وریج، دانشگاه بروکسل، بلژیک | ||
| چکیده [English] | ||
| Potamogeton و Stuckenia به ترتیب شامل 90 و 7 گونه پذیرفته شده در سراسر جهان هستند. به دلیل طیف وسیعی از تنوعات مورفولوژیکی درون گونه‌ای از جمله انعطاف‌پذیری فنوتیپی گسترده و تنوع زیست محیطی،Potamogeton و Stuckenia جنس‌های بسیار دشواری در نظر گرفته می‌شوند. در مجموع 100 نمونه به نمایندگی از 11 گونه از Potamogeton و سه گونه از Stuckenia از سراسر ایران جمع‌آوری شدند. غربالگری اولیه بر اساس طول آلل توالی‌هایcpDNA ، امکان در نظر گرفتن 59 نمونه مختلف از یک تا هشت فرد از هر گونه را برای آنالیزهای مورفولوژیکی، تشریحی و مولکولی بیشتر فراهم نمود. توالی‌های هسته‌ای و کلروپلاستی برای بازسازی درختان مولکولی با استفاده از RaxML و رویکرد بیزین استفاده شدند. آنالیزهای nrDNA وضوح بالاتری را نسبت به داده‌های کلروپلاستی، در واگرایی گونه‌ها نشان دادند. تعدادی ناهماهنگی بین داده‌های هسته‌ای و کلروپلاستی وجود داشت (P=0.001). آنالیزهای اضافه‌تر نشانگرهای ISSR و SRAP برای 48 نمونه، وضوح بالاتری را در تعیین حدود گونه‌ها در میان گونه‌های برگ خطی نشان داد، اگرچه قطعی نیست. ویژگی‌های تشریحی نمی‌توانند گونه‌های ایرانی Potamogeton را به‌تنهایی تفکیک کند و باید در ترکیب با خصوصیات ریخت‌شناسی که بسیار دربردارنده اطلاعات بودند استفاده شود. نتایج نشان دادند که گونه‌های Potamogeton ایرانی با توجه به شرایط خاص و متنوع جغرافیایی ایران دارای خزانه ژنتیکی غنی هستند. | ||
| کلیدواژه‌ها [English] | ||
| گیاهان آبزی, نشانگرهای مولکولی, هیبریداسیون, ITS, درختان مولکولی | ||
| مراجع | ||
|
Abbasi, S. & Afsharzadeh, S. 2016: An efficient and simple CTAB-based method for total genomic DNA isolation from low amounts of aquatic plants with a high level of secondary metabolites.–Progress in Biological Sciences 6 :1–12.
Abbasi, S., Afsharzadeh, S., Saeidi, H. & Triest, L. 2016: Strong Genetic Differentiation of Subconcatenated plant populations across mountain ranges: Evidence from Potamogeton pectinatus in Iran. –PLoS ONE 11(8): e0161889.
DOI: 10.1371/journal.pone.0161889
Abbasi, S., Afsharzadeh, S., Dinarvand, M. & Wiegleb, G. 2017: Synopsis of Potamogetonaceae in Iran with supplements to the Flora Iranica and a new identification key. –Phytotaxa: 311: 1–21.
Ascherson, P. & Graebner, P. 1907: Potamogetonaceae. In. Das Pflanzenreich: regni vegetabilis conspectus 31(IV.11), Engler, A. (e.), Wilhelm Engelmann, 1–183. –Leipzig.
Aykurt, C. & Deniz, I.G. 2016: Anatomical differences of the Turkish Stuckenia Börner (Potamogetonaceae) and their taxonomic significance. –Pakistan J. of Botany 48: 179–186.
Blair, M.W., Panaud, O. & MC Couch, S.R. 1999: Inter simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and finger printing in rice (Oriza sativa L.).–Theoretical and Applied Genetics 98: 780–792.
Cook, CDK. 1990: Aquatic Plant Book. SPB Academic Publishing, The Hague.
Cuenoud, P., Savolainen, V., Chatrov. L.W., Powell, M., Grayer, R.J. & Chase, M.W. 2002: Molecular phylogenetics of Caryophyllales based on nuclear 18S rDNA and plastid rbc L, atpB, and matK sequences. –American J. of Botany 89: 132–144.
Du, Z.Y., Qimike, A., Yang, C.F., Chen, J.M. & Wang, Q.F. 2011: Testing four barcoding markers for species identification of Potamogetonaceae. -Journal of Systematics and Evolution 49(3): 246–251.
Dinarvand, M. 2017: Families of Aquatic Plants. In: Assadi, M., Massoumi, A.A. (eds), Flora of Iran, Research Institute of Forest and Rangelands. Tehran, Iran, No. 101–123: 3–130. [In Persian].
Dinarvand, M., Assadi, M. & Abbasi, S. 2022: A Taxonomic Revision on Aquatic Vascular Plants in Iran. -Rostaniha 23(Supplement 2): 1-50.
Edgar, RC. 2004: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797.
DOI: 10.1093/nar/gkh340
Fant, J.B., Kamau, E. & Preston, C.D. 2003: Chloroplast evidence for the multiple origins of the hybrid Potamogeton × sudermanicus Hagstr. -Aquatic Botany 75: 531–356.
Fehrer, J., Gemeinholzer, B., Chrtek, J.J. & Bra¨utigam, S. 2007: Incongruent plastid and nuclear DNA phylogenies reveal ancient intergeneric hybridization in Pilosella hawkweeds (Hieracium, Cichorieae, Asteraceae). -Molecular Phylogenetics and Evolution 42: 347–361.
Fernald, M.L. 1932: The linear- leaved North American species of Potamogeton section Axillares. -Memoirs of the American Academy of Arts and Sciences 17: 1–183.
Hagström, J.O. 1916: Critical researches on the Potamogetons. Almqvist & Wiksells Boktryckeri-A.-B. 55: 1–281.
Haynes, R.R. 1974: A revision of North American Potamogeton subsection pusilli (Potamogetonaceae). -Rhodora 82: 564–649.
Haynes, R.R., Les, D.H. & Kral, M. 1998: Two new combinations in Stuckenia, the correct name for Coleogeton (Potamogetonaceae). -Novon 8: 241.
Hettiarachchi, P. & Triest, L. 1991: Isozyme polymorphism in the genus Potamogeton (Potamogetonaceae). -Opera Botanica Belgica 4: 87–114.
Holub, J. 1997: Stuckenia Boerner 1912: the correct name for Coleogeton (Potamogetonaceae). -Preslia 69: 361–366.
Hollingsworth, P.M., Preston, C.D. & Gornall, R.J. 1998: Euploid and aneuplpoid evolution in Potamogeton (Potamogetonaceae): a factual basis for interpretation. -Aquatic Botany 60: 337–358.
Iida, S., Kosuge, K. & Kadono, Y. 2004: Molecular phylogeny of Japanese Potamogeton species in light of noncoding chloroplast sequences. -Aquatic Botany 80: 115–127.
Ito, Y., Robledo, G.L, Iharlegui, L. & Tanaka, N. 2016: Phylogeny of Potamogeton (Potamogetonaceae) Revisited: Implications for Hybridization and Introgression in Argentina. -Bulletin of the National Museum of Nature and Science, Ser. B 42: 131–141.
Kaplan, Z. 2002: Phenotypic plasticity in Potamogeton (Potamogetonaceae). -Folia Geobotanica 37: 141–170.
Kaplan, Z. 2008: A taxonomic revision of Stuckenia (Potamogetonaceae) in Asia, with notes on the diversity and variation of the genus on a worldwide scale. -Folia Geobotanica 43: 159–234.
Kaplan, Z. & Fehrer, J. 2004: Evidence for the hybrid origin of Potamogeton× cooperi (Potamogetonaceae): traditional morphology-based taxonomy and molecular techniques in concert. -Folia Geobotanica 39: 431–453.
Kaplan, Z., Jarolimova, V. & Fehrer, J. 2013: Revision of chromosome numbers of Potamogetonaceae: a new basis for taxonomic and evolutionary implications. -Preslia 85: 421–482.
Kimura, M. 1980: A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. –Journal of Molecular Evolution. 16: 111–120.
Kress, W.J. & Erickson, D.L. 2007: A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. -PLoS One. 2: e508.
Kress, W.J., Wurdack, K.J., Zimmer, E.A., Weigt, L.A. & Janzen, D.H. 2005: Use of DNA barcodes to identify flowering plants. Proceedings of the NationalAcademy of Sciences, USA, 102: 8369–8374.
Leonard, J. 1988: Contribution à l’étude de la flore et de la végétation des desert d’Iran, Fascicule 8: Etude des aires de distribution -Les phytochories -Les chorotypes. Bulletin of the Jardin Botanique National de Belgique, Meise.
Les, D.H. 1983: Taxonomic implications of aneuploidy and polyploidy in Potamogeton (Potamogetonaceae). -Rhodora 85: 301–323.
Les, D.H. & Haynes, R.R. 1995: Systematics of subclass Alismatidae. A synthesis of approaches. In: Rudall, P.J., Cribb, P.J., Cutler, D.F., Humphries, C.J. (Eds.), Monocotyledons: Systematics and Evolution. Royal Botanic Gardens, Kew, pp. 353–377.
Les, D.H. & Sheridan, D.J. 1990: Biochemical heterophylly and flavonoid evolution in North American Potamogeton (Potamogetonaceae). -American J. of Botany 77: 453–465.
Levin, R.A., Wagner, W.L., Hoch, P.C., Nepokroeff, M., Pires, J.C., Zimmer, E.A. & Sytsma, K.J. 2003: Family‐level relationships of Onagraceae based on chloroplast rbcL and ndhF data. -American J. of Botany 90(1): 107-115.
Li, G. & Quiros, C.F. 2001: Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. -Theoretical and Applied Genetics 103: 455–461.
Lindqvist, C., De-Laet, J., Haynes, R.R., Aagesen, L., Keener, B.R. & Albert, V.A. 2006: Molecular phylogenetics of an aquatic plant lineage, Potamogetonaceae. -Cladistics 22: 568 –588.
Liu, Q.L., Ge, S., Tang, H.B., Zhang, X.L., Zhu, G.F. & Lu, B.R. 2006: Phylogenetic relationships in Elymus (Poaceae: Triticeae) based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. -New Phytologist Journal 170: 411–420.
Moallem, R. 2008: Investigation of intraspecies and interspecies diversity of the genus Potamogeton L. in Zayandehrood river based on morphological, molecular and ecological features. Master thesis of Biology. University of Isfahan.
Meier, R., Shiyang, K., Vaidya, G. & Ng, P.K.L. 2006: DNA barcoding and taxonomy in Diptera: a tail of high intraspecific variability and low identification success. -Systematic Biology 55: 715–728.
Nylander, J.A.A. 2008: MrModeltest 2.3. Program distributed by the author. Evolutionary Biology Centre Uppsala University.
Ogden, E.C. 1943: The broad-leaved species of Potamogeton. -Rhodora 45(531): 57-105.
Page, R.D. 1996: TreeView: an application to display phylogenetic trees on personal computers. -Computer Applications in the Biosciences 12: 357–358. Posada, D. & Buckley, T.R. 2004: Model selection and model averaging in phylogenetics: analysis of Akaike information criterion and Bayesian approaches over likelihood ratio tests. -Systematic Biology 53: 793–808.
POWO. 2023: Plants of the World Online. Facilitated by the Royal BotanicGardens, Kew. Published on the Internet: http://www. plantsoftheworldonline.org/(Retrieved 20 Novmber 2023) Raunkiaer, C. 1903: Anatomical Potamogeton-studies and Potamogeton fluitans. -Botanisk Tidsskrift 25: 253–380.
Rieseberg, L.H. & Soltis, D.E. 1991: Phylogenetic consequences of cytoplasmic gene flow in plants. -Evolutionary Trends in Plants. 5: 65–84 Rohlf, F.J. 2000: NTSYS-pc Numerical taxonomy and multivariate analysis system, v. 2.1. New York: Setauket.
Ronquist, F. & Huelsenbeck, J.P. 2003: MrBayes 3: Bayesian phylogenetic inference under mixed models. -Bioinformatics 19: 1572–1574.
Sack, L. & Scoffoni, C. 2013: Leaf venation: structure, function, development, evolution, ecology and applications in the past, present and future. -New Phytologist Journal 198: 983–1000.
Sang, T., Crawford, D.J. & Stuessy, T.F. 1997: Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). -American J. of Botany 84(9): 1120–1136.
Schlichting, C.D. 1986: The evolution of phenotypic plasticity in plants. –Annual Review of Ecology, Evolution and Systematics 17: 667–693
Stamatakis, A. 2006: RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.
Sun, K., Chen, X., Ma, R., Li, C., Wang, Q. & Ge, S. 2002: Molecular phylogenetics of Hippophae L. (Elaeagnaceae) based on the internal transcribed spacer (ITS) sequences of nrDNA. -Plant Systematic and Evolution 235: 121–134.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. 2011: MEGA5 molecular evolutionary genetics analysis using maximum likelihood evolutionary distance, and maximum parsimony methods. -Molecular Biology and Evolution 28: 2731–2739.
Tate, J.A. & Simpson, B. 2003: Paraphyly of Tarasa (Malvaceae) and diverse origins of the polyploid species. -Systematic Botany 28: 723-737.
Thompson, J.D., Higgins, D.G., Gibson, T.J. & Clustal, W. 1994: Improving the sensitivity of progressive weighting position-specific gap penalties and weight matrix choice. -Nucleic Acids Research 22: 4673–4680.
Tur, N.M. 1982: Revision del genero Potamogeton L. en la Argentina. -Darwiniana 24: 217-265.
Vari, A., Toth, V.R. & Csontos, P. 2010: Comparing the morphology of Potamogeton perfoliatus L. along environmental gradients in Lake Balaton (Hungary). -Annales de Limnologie-International Journal of Limnology 46: 111–119.
Wang, J.B., Wang, C., Shi, S.H. & Zhong, Y. 2000: ITS regions in diploids of Aegilops (Poaceae) and their phylogenetic implications. -Hereditas 132: 20–213.
Wang, Q.D., Zhang, T. & Wang, J.B. 2007: Phylogenetic relationship and hybrid origin of Potamogeton species (Potamogetonaceae) distributed in China: Insights from the nuclear ribosomal internal transcriber space sequencer (ITS). -Plant Systematic and Evolution 267: 65–78.
Wendelbo, P. 1971: Some distributional patterns within the flora Iranica, Pp. 29-41 in Davis, P.H. Harper, P.C. and Hedge, Ian C. (e.ds). Plant life of south-west Asia- The Botanical Society of Edinburg.
Whittall, J.B., Hellquist, C.B., Schneider, E.L. & Hodges, S.A. 2004: Cryptic species in an endangered pondweed community (Potamogeton, Potamogetonaceae) revealed by AFLP markers. -American J. of Botany 91: 2022–2029.
White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand, DH., Sninsky, JJ., White, TJ. eds. PCR protocols: a guide to methods and application. San Diego: Academic Press. 315–322.
Wiegleb, G. 1988: Notes on pondweeds: outlines for a monographical treatment of the genus Potamogeton L. -Feddes Repert. 99: 249–266.
Woolson, R.F. 2007: Wilcoxon signed‐rank test. Wiley encyclopedia of clinical trials, 1-3.
Wiegleb, G. & Kaplan, Z. 1998: An account of the species of Potamogeton L. (Potamogetonaceae). -Folia Geobotanica 33: 241–316.
Zhang, T., Wang, Q., Li, W., Cheng, Y. & Wang, J. 2008: Analysis of phylogenetic relationships of Potamogeton species in China based on chloroplast trnT-trnF sequences. -Aquatic Botany 89: 34–42.
Zietkiewicz, E., Rafalski, A. & Labuda, D. 1994: Genome fingerprinting by simple sequence repeat (SSR)- Anchored Polymerase Chain Reaction Amplification. -Genomics 20: 176–183.
Zohary, M. 1973: Geobotanical Foundations of the Middle East. Vol. 1-2, Gustav Fischer Verlag Press, Stuttgart, Swets & Zeitlinger, Amsterdam. | ||
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