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Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 20 آذر 1402 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2023.363578.2866 | ||
| نویسندگان | ||
| Hassan Saberi1؛ Nasrin Mehranfard2؛ Hossein rezazadeh3؛ Maedeh Ghasemi* 3 | ||
| 1School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran | ||
| 3Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Gastrointestinal dysfunction is a severe and common complication in diabetic patients. Some evidence shows that gamma-aminobutyric acid (GABA) and glutamate contribute to diabetic gastrointestinal abnormalities. Hence, we examined the impact of prolonged treatment with insulin and magnesium supplement on the expression pattern of GABA type A (GABA-A), GABA-B and N-methyl-D-aspartate (NMDA) glutamate receptors as well as nitric oxide synthase 1 (NOS-1) in the stomach of type 2 diabetic rats. Twenty-four male Wistar rats were randomized to 4 groups (6 rats each): 1) control, 2) type 2 diabetes: rats fed with a high fat diet for 3 months + low dose of streptozotocin (35 mg/kg), 3) type 2 diabetes + magnesium, and 4) type 2 diabetes + insulin. The expression of NOS-1 as well as GABA-A, GABA-B, and NMDA receptors was detected using western blotting. NOS-1 expression was substantially diminished (p<0.01) while the expression of GABA-A (p<0.001), GABA-B (p<0.001), and NMDA (p<0.001) receptors was enhanced in the stomach of diabetic rats relative to control. Treatment with magnesium and insulin improved NOS-1 expression in diabetic rats, although this effect was greater in magnesium treatment alone. Magnesium also restored the expression of GABA-A and GABA-B receptors in diabetic rats to control values. Moreover, insulin treatment improved GABA-A receptor expression in diabetic rats (p<0.05). No considerable alterations were detected in NMDA receptor level in the treatment groups. The results suggest a significant role of magnesium and insulin in improving gastric motility and secretory disorders associated with diabetes through modifying the expression of GABAergic receptors. | ||
| کلیدواژه‌ها | ||
| Type 2 Diabetes؛ Magnesium Supplement؛ GABA Receptor؛ NMDA Receptor؛ NOS-1 | ||
| عنوان مقاله [English] | ||
| دخالت رسپتورهای گاما-آمینوبوتیریک اسید و ان-متیل-دی-آسپارتات در گاستروپاتی دیابتی در موش صحرائی: اثرات سودمند درمان بلند مدت انسولین و مکمل منیزیم | ||
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