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Eshaghniya, Alireza, Haghbin Nazarpak, Hadi, Ghalyanchilangeroudi, Arash, Hosseini, Hossein. (1403). Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran. سامانه مدیریت نشریات علمی, 79(3), 575-586. doi: 10.32592/ARI.2024.79.3.575
Alireza Eshaghniya; Hadi Haghbin Nazarpak; Arash Ghalyanchilangeroudi; Hossein Hosseini. "Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran". سامانه مدیریت نشریات علمی, 79, 3, 1403, 575-586. doi: 10.32592/ARI.2024.79.3.575
Eshaghniya, Alireza, Haghbin Nazarpak, Hadi, Ghalyanchilangeroudi, Arash, Hosseini, Hossein. (1403). 'Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran', سامانه مدیریت نشریات علمی, 79(3), pp. 575-586. doi: 10.32592/ARI.2024.79.3.575
Eshaghniya, Alireza, Haghbin Nazarpak, Hadi, Ghalyanchilangeroudi, Arash, Hosseini, Hossein. Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran. سامانه مدیریت نشریات علمی, 1403; 79(3): 575-586. doi: 10.32592/ARI.2024.79.3.575

Evaluation of protective immunity in chickens vaccinated with combined IB H120/D274 and IB H120 against IS/1494/06 in Iran

Archives of Razi Institute
مقاله 17، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 575-586    اصل مقاله (1.13 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.575
نویسندگان
Alireza Eshaghniya1؛ Hadi Haghbin Nazarpak* 2؛ Arash Ghalyanchilangeroudi3؛ Hossein Hosseini4
1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran.
3Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
چکیده
Infectious bronchitis virus (IBV) has a variety of serotypes that cause many problems in the poultry industry. Two H120 and H120-D274 live vaccines were evaluated against strain IS/1494/06 (variant 2) infectious bronchitis virus challenge. The study aimed to determine whether it is possible to achieve success in controlling disease symptoms and pathological lesions and reducing virus shedding by combining two types of vaccines against different severities of the Poultry Infectious Bronchitis Virus. After random selection of 100 SPF chickens, four 25 chicks/group experimental groups were arranged in the H120 (IB-H120, Intervet®; Serial No: **** at 1st day + Booster 14th day + Challenged with IS/1494/06- 14 DPV), H120-D274 (Poulvac® IB Primer at 1st day + Booster 14th day + Challenged with IS/1494/06- 14 DPV), Control (No vaccine and No Challenge), and Challenged (No vaccine + Challenge). After euthanasia of the animals on the 42nd day of the research, serum neutralization (days 14, 28, and 42), ELISA (14 days after the booster vaccine and before challenged), Ciliostasis (7 days after the booster vaccine and seven days after challenged) and pathological lesion scores were evaluated on day 14 after being infected with IS/1494/06 type 2 strain (day 42). Also, the virus shedding was monitored by real-time RT-PCR. Infection with the IS/1494/06 variant2 strain showed high to moderate ciliostasis and pathologic scores. All groups that received the IB-H120-D274 vaccine had fewer lesions relatively. The IB-H120-D274 vaccinated groups showed the highest protection rates and high protection scores (70.3) compared to the IB-H120 vaccine groups (24.4). The virus shedding was significantly reduced in groups that received the H120-D274 vaccine compared to those that received the IB-H120 vaccine. In conclusion, the homologous IB-H120-D274 vaccine showed superior results compared to the IB-H120 vaccine.
کلیدواژه‌ها
IBV؛ combined vaccine؛ Serum neutralization test؛ ELISA؛ Real-time PCR
عنوان مقاله [English]
بررسی مقایسه ای مولکولی، سرولوژی و پاتولوژی واکسن ترکیبی IB-H120-D274 و Mass IB-H120 در برابر ویروس های برونشیت عفونی گونه 2 پرندگان جدا شده در ایران
چکیده [English]
ویروس برونشیت عفونی (IBV) دارای سروتیپ های مختلفی است که باعث ایجاد مشکلات زیادی در صنعت طیور می شود. دو واکسن زنده H120 و H120-D274 در برابر چالش ویروس برونشیت عفونی سویه IS/1494/06 (نوع 2) مورد ارزیابی قرار گرفتند. پس از انتخاب تصادفی 100 جوجه SPF، چهار گروه آزمایشی 25 جوجه/گروه در گروه های کنترل و چالش شده (بدون دریافت واکسن)، H120 (واکسینه شده علیه سویه IB-H120 در روز اول و سویه IB-793B در روز ۱۴) و H120-D274 (واکسینه شده علیه سویه های IB-H120-D274 در روز اول، و سویه IB-793B در روز 14) قرار گرفتند. همه گروه ها به جز گروه شاهد با سویه IS/1494/06 variant2 داخل چشمی در 14 روز پس از واکسیناسیون بوستر(روز 28) آلوده شدند. پس از اتانازی حیوانات در روز 42 تحقیق، آزمایشات خنثی سازی سرم (روزهای 14، 28 و 42)، الایزا (14 روز پس از واکسن بوستر، قبل از چالش)، سیلیوستاز (7 روز پس از واکسن بوستر و هفت روز پس از واکسن بوستر). به چالش کشیده شدند و نمرات ضایعه پاتولوژیک در روز 14 پس از آلوده شدن به سویه IS/1494/06 نوع 2 (روز 42) مورد ارزیابی قرار گرفت. همچنین، ریزش ویروس توسط RT-PCR بلادرنگ پایش شد. عفونت با سویه IS/1494/06 variant2 نمرات مژگانی و پاتولوژی بالا تا متوسط را نشان داد. تمام گروه هایی که واکسن IB-H120-D274 دریافت کردند ضایعات نسبتاً کمتری داشتند. گروه های واکسینه شده IB-H120-D274 بالاترین میزان حفاظت و امتیازات حفاظتی بالایی (70.3) را در مقایسه با گروه های واکسن IB-H120 (24.4) نشان دادند. ریزش ویروس در گروه هایی که واکسن H120-D274 را دریافت کرده بودند در مقایسه با گروه هایی که واکسن IB-H120 دریافت کردند به طور قابل معنی داری کاهش یافت. در نتیجه، واکسن همولوگ IB-H120-D274 نتایج بهتری را در مقایسه با واکسن IB-H120 نشان داد.
کلیدواژه‌ها [English]
IBV, واکسن ترکیبی, تست خنثی سازی سرم, الایزا, Real-time PCR
سایر فایل های مرتبط با مقاله
مراجع
  1. Cook JK. Coronaviridae. Poultry diseases. 2008:340.
  2. Park J, Jung JS, Lee R, Yoon S-i, Lee G-S, Sung HW, Kwon HM. Genetic and Immunological Characterization of Commercial Infectious Bronchitis Virus (IBV) Vaccines Used in Korea. 2022.
  3. Hoerr FJ. The Pathology of Infectious Bronchitis. Avian Dis. 2021;65(4):600-11.
  4. Ting X, Xiang C, Liu DX, Chen R. Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit. Frontiers in Microbiology. 2022;13:897560.
  5. Timurkaan N, ÖNGÖR H, Kalender H, Karabulut B, ÇÖVEN F, ÇEVİK A, et al. Pathological and molecular findings of visceral gout caused by Israel variant 2 (IS/1494/06) genotype of infectious bronchitis virus in chickens. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2023;70(2):149-56.
  6. Khan SZ, Waqas M, Goyal SM. Infectious Bronchitis: A Challenge for the Global Poultry Industry. Sarhad Journal of Agriculture. 2023;39(2).
  7. Al-Rasheed M, Ball C, Manswr B, Leeming G, Ganapathy K. Infectious bronchitis virus infection in chicken: viral load and immune responses in Harderian gland, choanal cleft and turbinate tissues compared to trachea. British Poultry Science. 2022;63(4):484-92.
  8. Jung JS, Lee R, Yoon S-i, Lee G-S, Sung HW, Kwon HM, Park J. Genetic and immunological characterization of commercial infectious bronchitis virus vaccines used in Korea. Archives of Virology. 2022;167(11):2123-32.
  9. Hosseini H, Fard MH, Charkhkar S, Morshed R. Epidemiology of Avian Infectious Bronchitis Virus Genotypes in Iran (2010-2014). Avian Dis. 2015;59(3):431-5.
  10. Smialek M, Tykalowski B, Dziewulska D, Stenzel T, Koncicki A. Immunological aspects of the efficiency of protectotype vaccination strategy against chicken infectious bronchitis. BMC Vet Res. 2017;13(1):44.
  11. Habibi M, Karimi V, Langeroudi AG, Ghafouri SA, Hashemzadeh M, Farahani RK, et al. Combination of H120 and 1/96 avian infectious bronchitis virus vaccine strains protect chickens against challenge with IS/1494/06 (variant 2)-like infectious bronchitis virus. Acta Virol. 2017;61(2):150-60.
  12. Cook JK, Orbell SJ, Woods MA, Huggins MB. Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes. Avian Pathol. 1999;28(5):477-85.
  13. Masoudi S, Pishraft Sabet L, Shahsavadi S. Immunogenicity and Efficacy of Live Infectious Bronchitis 793/B.08IR Vaccine in SPF Chickens. Archives of Razi Institute. 2020;75(1):23-30.
  14. Gu K, Song Z, Ma P, Liao Z, Yang M, Zhou C, et al. A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken Serum. Int J Mol Sci. 2022;23(14).
  15. Ismail ZM, El-Deeb AH, El-Safty MM, Hussein HA. Enhanced pathogenicity of low-pathogenic H9N2 avian influenza virus after vaccination with infectious bronchitis live attenuated vaccine. Vet World. 2018;11(7):977-85.
  16. Alvarado I, Villegas P, El-Attrache J, Brown T. Evaluation of the protection conferred by commercial vaccines against the California 99 isolate of infectious bronchitis virus. Avian diseases. 2003;47(4):1298-304.
  17. Ratanasethakul C, Cumming R. The effect of route of infection and strain of virus on the pathology of Australian infectious bronchitis. Australian Veterinary Journal. 1983;60(7):209-13.
  18. Cavanagh D. Coronavirus avian infectious bronchitis virus. Vet Res. 2007;38(2):281-97.
  19. Raj GD, Jones RC. Cross-reactive cellular immune responses in chickens vaccinated with live infectious bronchitis virus vaccine. Avian Pathol. 1997;26(3):641-9.
  20. Gong H, Ni R, Qiu R, Wang F, Yan W, Wang K, et al. Evaluation of a novel recombinant strain of infectious bronchitis virus emerged from three attenuated live vaccine strains. Microbial Pathogenesis. 2022;164:105437.
  21. Bru T, Vila R, Cabana M, Geerligs HJ. Protection of chickens vaccinated with combinations of commercial live infectious bronchitis vaccines containing Massachusetts, Dutch and QX-like serotypes against challenge with virulent infectious bronchitis viruses 793B and IS/1494/06 Israel variant 2. Avian Pathology. 2017;46(1):52-8.
  22. Elhady MA, Ali A, Kilany WH, Elfeil WK, Ibrahim H, Nabil A, et al. Field Efficacy of an Attenuated Infectious Bronchitis Variant 2 Virus Vaccine in Commercial Broiler Chickens. Veterinary Sciences. 2018;5(2):49.
  23. Motamed N, Bashashati M. Comparative genetic features of S1 gene from six Massachusetts and variant avian 2 infectious bronchitis viruses isolated in Iran. Archives of Razi Institute. 2022;77(5):1611-9.
  24. de Wit JJS, Malo A, Cook JKA. Induction of IBV strain-specific neutralizing antibodies and broad spectrum protection in layer pullets primed with IBV Massachusetts (Mass) and 793B vaccines prior to injection of inactivated vaccine containing Mass antigen. Avian Pathol. 2019;48(2):135-47.
  25. Bagherpour Sorkhabi S, Shayegh J, Hosseini H, Ghalyanchilangeroudi A. Effects of a combination of Mass and Dutch variant as an inactivated vaccine against variant 2 avian infectious bronchitis virus challenge. Microb Pathog. 2021;156:104937.
  26. Awad F, Forrester A, Baylis M, Lemiere S, Ganapathy K, Hussien HA, Capua I. Protection conferred by live infectious bronchitis vaccine viruses against variant Middle East IS/885/00‐like and IS/1494/06‐like isolates in commercial broiler chicks. Veterinary record open. 2015;2(2):e000111.
  27. Lambrechts C, Pensaert M, Ducatelle R. Challenge experiments to evaluate cross‐protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virus. Avian Pathology. 1993;22(3):577-90.
  28. Karimi V, Mohammadi P, Ghalyanchilangeroudi A, Ghafouri SA, Hashemzadeh M, Farahani RK, et al. Including 793/B type avian infectious bronchitis vaccine in 1-day-old chicken increased the protection against QX genotype. Tropical Animal Health and Production. 2019;51(3):629-35.
  29. Al-Rasheed M, Shawky M. The Role of Head Associated Lymphoid Tissues in Infectious Bronchitis Virus. World. 2023;13(1):01-11.
  30. Blakey J, Crossley B, Da Silva A, Rejmanek D, Jerry C, Gallardo RA, Stoute S. Infectious bronchitis virus associated with nephropathy lesions in diagnostic cases from commercial broiler chickens in California. Avian Diseases. 2020;64(4):482-9.
  31. Abdel-Sabour MA, Rohaim MA, Salman OJ, Abodalal SE, Mohammad FF, Madkour MS, et al. Immunogenicity and efficacy of a bivalent vaccine against infectious bronchitis virus. Comparative Immunology, Microbiology and Infectious Diseases. 2021;77:101670.
  32. Xie J, Meng X, Zhang J, Xie Q, Zhang W, Li T, et al. A novel S2-derived peptide-based ELISA for broad detection of antibody against infectious bronchitis virus. Poultry Science. 2023;102(6):102661.
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