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بهبود جوانهزنی، رشد و خصوصیات بیوشیمیایی گیاهچه‌های ذرت هیبرید سینگل کراس 704 با کاربرد نانوذرات نقره سنتز شده از گیاه رازیانه (Foeniculum vulgare) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 2، دوره 13، شماره 1 - شماره پیاپی 33، اسفند 1402، صفحه 15-36 اصل مقاله (521.09 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2023.361213.1471 | ||
| نویسندگان | ||
| مهدی افروز1؛ پریسا شیخ زاده* 2 | ||
| 1گروه آموزشی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی | ||
| 2گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| چکیده | ||
| به‌منظور ارزیابی تأثیر نانوذرات نقره سنتز شده از رازیانه بر جوانهزنی بذر، رشد و خصوصیات بیوشیمیایی گیاهچه‌های ذرت هیبرید سینگل کراس 704، آزمایشی به‌صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1400 اجرا شد. تیمار‌های آزمایش شامل غلظت‌های مختلف نانوذرات نقره (صفر، 001/0، 1/0، 25/0 و 75/0 میلیگرم در لیتر) و روش کاربرد (پیشتیمار بذر و افزودن به بستر کشت) بود. نتایج نشان داد که در هر دو روش کاربرد نانوذره، با کاربرد غلظت‌های مختلف نانوذرات نقره موجب افزایش معنی‌دار درصد و سرعت جوانهزنی، متوسط جوانهزنی روزانه، شاخص همزمانی جوانهزنی، شاخص قدرت، طول و وزن خشک گیاهچه‌های ذرت و کاهش میانگین مدت جوانهزنی، زمان تا 50 درصد جوانهزنی بذرهای ذرت گردید. از میان روش‌های کاربرد نانوذره، پیشتیمار کردن بذرها با 001/0 میلیگرم در لیتر و افزودن 1/0 میلیگرم در لیتر نانوذره نقره، تأثیر بیشتری در بهبود جوانهزنی، رشد گیاهچهها و افزایش شاخص قدرت بذر داشت. با افزودن 1/0 میلیگرم در لیتر نانوذره نقره به بستر کاشت، سبب شد تا بیشترین درصد جوانهزنی، شاخص همزمانی، متوسط جوانهزنی روزانه و کمترین میانگین مدت جوانهزنی حاصل شود. همچنین کاربرد غلظت‌های مختلف نانوذره نقره در هر دو روش کاربرد موجب افزایش میزان فعالیت آنزیم‌های کاتالاز، پراکسیداز، پلی‌فنول‌اکسیداز و محتوی اسید‌آمینه پرولین نسبت به تیمار شاهد گردید. به‌طور کلی کاربرد غلظت 001/0 میلیگرم در لیتر نانوذره نقره به‌روش پیشتیمار بذر و افزودن غلظت 1/0 میلیگرم در لیتر نانوذره به بستر کاشت جهت بهبود جوانهزنی بذر، رشد و خصوصیات بیوشیمیایی گیاهچه‌های ذرت پیشنهاد می‌شود. | ||
| کلیدواژه‌ها | ||
| پیشتیمار بذر؛ سنتز سبز؛ سرعت جوانهزنی؛ فعالیت آنزیم‌های آنتی‌اکسیدان | ||
| عنوان مقاله [English] | ||
| Improving seed germination, growth, and biochemical characteristics of corn seedling via the application of silver nanoparticles synthesized from fennel (Foeniculum vulgare) | ||
| نویسندگان [English] | ||
| Mehdi Afrouz1؛ Parisa Sheikhzadeh2 | ||
| 1Department of Plant Production and Genetic Engineering University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| چکیده [English] | ||
| In order to evaluate the effect of Ag synthesized from fennel on seed germination, growth and biochemical characteristics of hybrid single cross 704 corn seedlings, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili in 2021. Experimental factors included synthesized Ag nanoparticles (0, 0.001, 0.1, 0.25, and 0.75 mg L-1) and the application methods of Ag nanoparticles (seed priming and no priming). The results showed that in both methods of nanoparticle application, with the use of different concentrations of Ag nanoparticles, there was a significant increase in the percentage and speed of germination, average daily germination, germination simultaneity index, strength index, length and dry weight of corn seedlings and a decrease in the average germination time, D50 of corn seeds were germinated. Among the nanoparticle application methods, priming seeds with 0.001 mg L-1 and adding 0.1 mg L-1 Ag nanoparticles had a greater effect on improving germination, seedling growth, and increasing seed vigor index. By adding 0.1 mg L-1 of Ag nanoparticles to no priming, it resulted in the highest germination%, synchronicity index, mean daily germination and the lowest mean germination time. The use of different concentrations of Ag nanoparticles in both application methods increased the activity of catalase, peroxidase, polyphenol oxidase and proline content compared to the control treatment, the use of a concentration of 0.001 mg L-1 of Ag nanoparticles as a priming can be suggested to improve seed germination, growth, and biochemical characteristics of corn seedlings. | ||
| کلیدواژه‌ها [English] | ||
| Seed pretreatment, Green synthesis, Germination rate, Antioxidant enzymes activity | ||
| مراجع | ||
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