| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,139 |
| تعداد مقالات | 34,918 |
| تعداد مشاهده مقاله | 50,383,131 |
| تعداد دریافت فایل اصل مقاله | 90,276,531 |
Parasitological and molecular study of nosemosis in migratory apiaries in Hormozgan Province, southern Iran | ||
| Journal of Entomological Society of Iran | ||
| مقاله 6، دوره 43، شماره 4، بهمن 2023، صفحه 383-392 اصل مقاله (1.18 M) | ||
| نوع مقاله: Paper, English | ||
| شناسه دیجیتال (DOI): 10.61186/jesi.43.4.6 | ||
| نویسندگان | ||
| Bahareh Meftahi1؛ Saeed Yaghfoori2؛ Sadegh Mosazadeh2؛ Reza Sheibanitezerji2؛ Mostafa Fakhrabadipour2؛ Esmaeil Javdan2؛ Gholamreza Razmi* 1 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Hormozgan Veterinary Head Office, Bandar Abbas, Iran | ||
| چکیده | ||
| Nosemosis is a microsporidian disease caused by Nosema ceranae and N. apis and transmitted via oral-fecal and oral-oral routes. It is globally distributed among adult bees in honeybee colonies. Considering the health importance of nosemosis in honeybees, the study aims to determine the frequency of Nosema spp. infection in migratory apiaries in Hormozgan province by microscopic and molecular methods. In the present study, 20 bees from ten randomly selected hives in 84 migratory apiaries were collected. In the laboratory, the abdomen of the bees was separated from the rest of the body with entomological tweezers and scissors and then ground up in a mortar containing saline serum. The prepared suspension was filtered by passing through a sieve, then the prepared suspension was transferred to test tubes and centrifuged. The pellets were repeatedly washed by saline solution and centrifuged. Finally, the pellets were examined for spores of Nosema spp. by light microscopy and conventional PCR. In microscopy, 38.2 % of apiaries were positive for Nosema spp. spores. By PCR however, DNA of N. ceranae was detected in 39.2. % of apiaries with no samples positive for N. apis. Due to the considerable frequency of infection in migratory apiaries in Hormozgan province, it is necessary to carry out appropriate health measures such as screening of apiaries with appropriate diagnostic methods and training of beekeepers to disinfect hives in order to control Nosema infection in Iranian apiaries by the veterinary health officials. | ||
| کلیدواژهها | ||
| Insect pathology, honey bee؛ Iran؛ characterization | ||
| عنوان مقاله [English] | ||
| مطالعه انگل شناسی و ملکولی نوزموزیس در زنبورستان های مهاجر در استان هرمزگان ،جنوب ایران | ||
| نویسندگان [English] | ||
| بهاره مفتاحی1؛ سعید یغفوری2؛ صادق موسی زاده2؛ رضا شیبانی طزرجی2؛ مصطفی فخرابادی پور2؛ اسماعیل جاودان2؛ غلامرضا رزمی1 | ||
| 1گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد ، ایران | ||
| 2اداره کل دامپزشکی استان هرمزگان، بندر عباس، ایران | ||
| چکیده [English] | ||
| نوزموزیس یک بیماری میکروسپوریدیایی ناشی از Nosema ceranae و Nosema apis است که باعث آلودگی زنبوران بالغ در کلنی های زنبورعسل در سراسر جهان می گردد. انتقال اسپورهای نوزما از طریق مسیرهای مدفوعی-دهانی و دهانی– دهانی اتفاق می افتد. با توجه به اهمیت سلامت زنبورهای عسل، هدف از این مطالعه بررسی فراوانی آلودگی با نوزما در زنبوداری های مهاجر به استان هرمزگان با استفاده از روشهای میکروسکوپی و مولکولی بود. بدین منظور از 84 زنبورستان مهاجر در استان هرمزگان نمونه برداری شد، بدین صورت که از هر زنبورستان 10 کندو انتخاب شده و از هر کندو 20 تا 30 زنبورعسل مسن به صورت تصادفی انتخاب شده و به آزمایشگاه منتقل شدند. در آزمایشگاه، شکم زنبورها با پنس و قیچی حشره شناسی از بقیه بدن جدا شده و در هاون حاوی سرم نمکی آسیاب شد. سوسپانسیون تهیه شده با عبور از الک صاف شده و به لوله های آزمایش منتقل و سانتریفیوژ گردید. رسوب ته لوله های ازمایش به طور مکرر با افزودن سرم فیزیولوژی و سانتریفیوژ شسته شده و برای تشخیص عفونت نوزما با روشهای میکروسکوپی و مولکولی مورد بررسی قرار گرفتند. در بررسی میکروسکوپی، اسپورهای گونه هایNosema در 38.2 درصد زنبورستان ها مشاهده شدند. با روش PCR اما 39.2 درصد زنبورستان ها آلوده به N. ceranae تشخیص داده شدند. هیچ موردی از آلودگی N. apis مشاهده نشد. با توجه به فراوانی قابل ملاحظه ی آلودگی نوزما در زنبورستان های مهاجر به استان هرمزگان، انجام اقدامات بهداشتی مناسب همچون غربال گری زنبوستان های کشوز با روشهای تشخیصی مناسب و آموزش زنبورداران جهت ضدعفونی صحیح کندوها برای کنترل بیماری در زنبورستان های ایران توسط مسئولان بهداشتی دامپزشکی ضروری است. | ||
| کلیدواژهها [English] | ||
| بیماری شناسی حشرات, زنبور عسل, ایران, تعیین مشخصات | ||
| مراجع | ||
|
Ansari, M. J., Al-Ghamdi, A., Nuru, A., Khan, K. A. & Alattal, Y. (2017) Geographical distribution and molecular detection of Nosema ceranae from indigenous honeybees of Saudi Arabia. Saudi Journal of Biological Sciences 24(5), 983–991. https://doi.org/10.1016/j.sjbs.2017.01.054
Aroee, F., Azizi, H., Shiran, B. & Pirali Kheirabadi, K. (2017) Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran). Asian Pacific Journal of Tropical Biomedicine, 7, 10–139. https://doi.org/10.1016/j.apjtb.2016.11.004
Bailey, L. & Ball, B. V. (1981) Honeybee Pathology. Academic Press, London.
Fries, I. (2010) Nosema ceranae in European honeybees (Apis mellifera). Journal of Invertebrate Pathology 103(1), S73–S79. https://doi.org/10.1016/j.jip.2009.06.017
Fries, I., Feng, F., Silva, A. D., Slemenda, S. B. & Pieniazek, N. J. (1996) Nosema ceranae n. sp. (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honeybee Apis cerana (Hymenoptera, Apidae). European Journal of Protistology 32(3), 356–365. https://doi.org/10.1016/S0932-4739(96)80059-9
Galajda, R., Valenčáková, A., Sučik, M. & Kandráčová, P. (2021) Nosema disease of European honeybees. Journal of Fungi (Basel). 7(9), 714. https://doi.org/10.3390/jof7090714
Giersch, T., Berg, T., Galea, F. & Hornitzky, M. (2009) Nosema ceranae infects honeybees (Apis mellifera) and contaminates honey in Australia. Apidologie, 40(2), 117–123 https://doi.org/10.1051/apido/2008065
Higes, M., Martín, R. & Meana, A. (2006) Nosema ceranae, a new microsporidian parasite in honey bees in Europe. Journal of Invertebrate Pathology 92(2),93–95. https://doi.org/10.1016/j.jip.2006.02.005
Higes, M., Martín-Hernández, R., Garrido-Bailón, E., Botías, C. & Meana, A. (2009) The presence of Nosema ceranae (microsporidia) in North African honeybees (Apis mellifera intermissa) Journal of Apicultural Research 48(3),217 –219. https://doi.org/10.3896/IBRA.1.48.3.12
Huang, W. F., Solter, L., Aronstein, K. & Huang, Z. (2015). Infectivity and virulence of Nosema ceranae and Nosema apis in commercially available North American honeybees. Journal of Invertebrate Pathology, 124, 107–113. https://doi.org/10.1016/j.jip.2014.10.006
Human, H., Brodschneider, R., Dietemann, V., Dively, G., Ellis, J. D., Forsgren, E., et al (2013). Miscellaneous standard methods for Apis mellifera research. Journal of Apicultural Research 52(4),1–53. https://doi.org/10.3896/IBRA.1.52.4.10
Imani- Baran, A., Hamidiam, G. (2022). A comparative study of the frequency and intensity of nosemosis based on individual and composite samples of live bees in the apiaries of the cities of East Azerbaijan province. Veterinary Clinical Pathology 16(63), 269-280. https://doi.org/10.30495/JVCP.2023.1964566.1376
Ivgin -Tunca, R., Oskay, D., Gosterit, A. & Tekin, O. K. (2016) Does Nosema ceranae wipe out Nosema apis in Turkey? Iranian Journal of Parasitology 11(2), 259–264.
Kareem, J. A., Kareem, A. A. & Shaher, K. W (2021) Detection of Nosema Cerranain samples of Iraqi bees using traditional and molecular methods. Annals of the Romanian Society for Cell Biology 24, 3008–30013.
Jabal-Uriel, C., Barrios, L., Bonjour-Dalmon, A., Caspi-Yona, S., Chejanovsly, N., Erez, T. & Martín-Hernández, R. (2022) Epidemiology of the microsporidium Nosema ceranae in four mediterranean countries. Insects 13(9), 844. https://doi.org/10.3390/insects13090844
Khezri, M., Moharrami, M., Modirrousta, H., Torkaman, M., Salehi, S., Rokhzad, B. & Khanbabi, H. (2018). Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran. Veterinary Research Forum 9(3),273–278. https://doi.org/10.30466/vrf.2018.32086
Lotfi, A., Jamshidi, R., Aghdam Shahryar, H. & Yousefkhani, M. (2009) The prevalence of nosemosis in honey bee colonies in the Arasbaran region (Northwestern Iran). Journal of Agriculture Environmental Science 5, 255–257.
Martín-Hernández, R., Meana, A., Prieto, L., Salvador, A.M., Garrido-Bailón, E. & Higes, M. (2007). The outcome of colonization of Apis mellifera by Nosema ceranae. Applied Environmental Microbiology 73(20),6331–6338. https://doi.org/10.1128/AEM.00270-07
Martín-Hernández, R., Bartolomé, C., Chejanovsky, N., Le Conte, Y., Dalmon, A., Dussaubat, C., García-Palencia, P., Meana, A., Pinto, M. A., Soroker, V. & Higes, M. (2018) Nosema ceranae in Apis mellifera: A 12 years postdetection perspective. Environmental Microbiology 20(4),1302 –1329. https://doi.org/10.1111/1462-2920.14103
Medici, S. K., Sarlo, E. G., Porrini, M. P., Braunstein. M. & Eguaras, M. J. (2012) Genetic variation and widespread dispersal of Nosema ceranae in Apis mellifera apiaries from Argentina. Parasitology Research 110,859–864 https://doi.org/10.1007/s00436-011-2566-2
Modirrousta, H., Moharrami M. & Mansouri, M. (2014) Retrospective study of the Nosema ceranae infection of honeybee colonies in Iran (2004-2013). Archives Razi Institute 69(2),197–200.
Mohammadian, B., Bokaie, S., Moharrami, M., Nabian, S. & Forsi, M. (2018) Distribution of Nosema spp. in climatic regions of Iran. Veterinary Research Forum 9(3),259–263. https://doi.org/10.30466/vrf.2018.32082
Moeini, S., Malekifard F. & Tavassoli, M. (2022) Identification of the Nosema spp., a microsporidian parasite isolated from the honeybees (Apis mellifera) and its association with honeybee colony losses in apiaries of Iran. Journal of the Hellenic Veterinary Medical Society 73(1),3667 –3672.
Moshverinia, A., Abedi, V., Safaie, H. (2012) A survey of Nosema apis infection in apiaries of North Khorasan province, Iran. Iranian Journal of Veterinary Science and Technology 4, 25–30. https://doi.org/10.22067/veterinary.v4i2.17418
Nabian, S., Ahmadi, K., Shirazi, M. N. & Gerami Sadeghian, A. (2011) First detection of Nosema ceranae, a microsporidian protozoon of European honeybees (Apis mellifera) in Iran. Iranian Journal of Parasitology 6(3), 89–95.
WOAH (World Organisation for Animal Health) (2019) Manual for diagnostic tests and vaccines for terrestrial animals, Nosemosis of honeybees, Paris.
Özgör, E., Güzerin, E. & Keskin, N. (2015). Determination and comparison of Nosema apis and Nosema ceranae in terms of geographic and climatic factors. Hacettepe Journal of Biology and Chemistry 43(1), 9–15.
Papini, R., Mancianti, F., Canovai, R., Cosci, F., Rocchigiani, G., Benelli, G. & Canale, A. (2017) Prevalence of the microsporidian Nosema ceranae in honeybee (Apis mellifera) apiaries in central Italy. Saudi Journal of Biological Sciences 24 (5),979–998. https://doi.org/10.1016/j.sjbs.2017.01.010
Petrie, A. & Watson P. (2006) Statistics for veterinary and animal science. Blackwell, Oxford.
Razmaraii, N., Sadegh-Eteghad, S., Babaei, H., Paykari, H., Esmaeilnia, K. & Froghy, L. (2013) Molecular identification of Nosema species in East Azerbaijan province, Iran. Archives Razi Institute 68, 23–27.
Safa, O., Soltanpoor, M., A., Rastegar, S., Kazemi, M., Nourbakhsh, K. & Ghanndi, A. (2012) An ethnobotanical survey on Hormozgan province, Iran. Avicenna Journal of Phytomedicine 31, 64-81.
Shirzadi, A. & Razmi, G. (2021). A microscopy and molecular studies of Nosema ceranae infection in Mazandaran province of Iran. Uludağ Arıcılık Dergisi 21(2), 198–205. https://doi.org/10.31467/uluaricilik.991579
Teixeira, E. W, dos Santos, L. G., Sattler, A., Message D., Alves, M. L. T. M. F., Martins M.F, et al. (2013) Nosema ceranae has been present in Brazil for more than three decades infecting Africanized honeybees. Journal of Invertebrate Pathology 114, 250–254. https://doi.org/10.1016/j.jip.2013.09.002
Tavassoli, M., Eiganinejad, S. & Alizadeh-Asl, S. (2009) A survey on Nosema apis infection in apiaries of Urmia, North-West of Iran. Iranian Journal of Veterinary Science and Technology 1(1), 35–40. https://doi.org/10.22067/veterinary.v1i1.2271
Thursfield, M. V. (1986). Veterinary Epidemiology, Butterworth, Guilford, London.
Ütük, A. E., Aliyeva, R., Girisgin, A. O., Gökmen, T. G., Özüiçli, M. & Aydın, L. (2019). First molecular detection of Nosema ceranae in Azerbaijan. Journal of Apicultural Research 58(4),559–561. https://doi.org/10.1080/00218839.2019.1614737
Vejnovic, B., Stevanovic. J., Schwarz. R. S., Aleksic. N., Mirilovic. M., Jovanovic, N. M. & Stanimirovic, Z. (2017) Quantitative PCR assessment of Lotmaria passim in Apis Mellifera colonies co-infected naturally with Nosema ceranae. Journal of Invertebrate Pathology 151, 76–81. https://doi.org/10.1016/j.jip.2017.11.003
Wang, Q., Dai, P., Guzman-Novoa, E., Wu, Y., Hou, C. & Diao, Q. (2019) Nosema ceranae, the most common microsporidium infecting Apis mellifera in the main beekeeping regions of China since at least 2005. Journal of Apicultural Research 58, 562–566. https://doi.org/10.3390/vetsci8060107
Williams, G. R., Shafer, A. B., Rogers, R. E., Shutler, D. & Stewart, D. T. (2008) First detection of Nosema ceranae, a microsporidian parasite of European honeybees (Apis mellifera), in Canada and central USA. Journal of Invertebrate Pathology 97(2), 189 –192. https://doi.org/10.1016/j.jip.2007.08.005 | ||
|
آمار تعداد مشاهده مقاله: 515 تعداد دریافت فایل اصل مقاله: 712 |
||