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Zakikhani, Farnaz, Afshar, Saman, Fattahi, Hossein, Taghinejad, Javid. (1403). Isolation and molecular identification of Deoxynivalenol and Fumonisin producing genes from maize feed contaminated with Fusarium fungus in silos of dairy farms in Fars province-Iran. سامانه مدیریت نشریات علمی, 79(3), 491-498. doi: 10.32592/ARI.2024.79.3.491
Farnaz Zakikhani; Saman Afshar; Hossein Fattahi; Javid Taghinejad. "Isolation and molecular identification of Deoxynivalenol and Fumonisin producing genes from maize feed contaminated with Fusarium fungus in silos of dairy farms in Fars province-Iran". سامانه مدیریت نشریات علمی, 79, 3, 1403, 491-498. doi: 10.32592/ARI.2024.79.3.491
Zakikhani, Farnaz, Afshar, Saman, Fattahi, Hossein, Taghinejad, Javid. (1403). 'Isolation and molecular identification of Deoxynivalenol and Fumonisin producing genes from maize feed contaminated with Fusarium fungus in silos of dairy farms in Fars province-Iran', سامانه مدیریت نشریات علمی, 79(3), pp. 491-498. doi: 10.32592/ARI.2024.79.3.491
Zakikhani, Farnaz, Afshar, Saman, Fattahi, Hossein, Taghinejad, Javid. Isolation and molecular identification of Deoxynivalenol and Fumonisin producing genes from maize feed contaminated with Fusarium fungus in silos of dairy farms in Fars province-Iran. سامانه مدیریت نشریات علمی, 1403; 79(3): 491-498. doi: 10.32592/ARI.2024.79.3.491

Isolation and molecular identification of Deoxynivalenol and Fumonisin producing genes from maize feed contaminated with Fusarium fungus in silos of dairy farms in Fars province-Iran

Archives of Razi Institute
مقاله 7، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 491-498    اصل مقاله (351.77 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.491
نویسندگان
Farnaz Zakikhani1؛ Saman Afshar2؛ Hossein Fattahi3؛ Javid Taghinejad* 4
1Department Researcher of Microbiology, Rasa Gene Company, Shiraz, Iran
2Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
3Department of Microbiology,Kazerun Branch, Islamic Azad University, Kazerun, Iran
4Department of Microbiology, Malekan Branch, Islamic Azad University, Malekan, Iran
چکیده
Mycotoxins are the toxins produced by various types of fungi, including Fusarium, which can produce different types of mycotoxins such as Deoxynivalenol (DON), Zearalenone (ZEN), T-2 toxin, and Fumonisins (FUM). Mycotoxins have the potential to reduce the quality of crops and pose health risks to both humans and animals. This can result in reduced animal production and substantial economic consequences on a global scale. Extensive research has been carried out to investigate the high incidence of contamination in grains by Fusarium fungi. In this study, 80 samples of maize from silos of dairy farms in Fars province, Iran were taken and examined for fungal contamination by Fusarium and potential production of DON and FUM. For this purpose, identification using specific primers for different genes was carried out by PCR test and gel electrophoresis with agarose (1%). Among different counties, the silos in Kharameh with 47.05% and Jahrom with 46.15% had the highest contamination rate, and the lowest contamination rate belonged to Kazeroon with 27.27%. Out of the 30 positive samples contaminated by Fusarium, 21 produced FUM, four produced DON, and five produced both toxins. Fusarium species that contaminated the maize samples were also identified, including 13, 10, and 7 samples contaminated by F. proliferatum, F. verticillioides, and F. graminearum, respectively. As a conclusion, the findings of the study indicate that 37.5% of the corn samples from dairy farm silos in Fars province were contaminated with the Fusarium fungus, which had the potential to produce the toxic mycotoxins, deoxynivalenol and fumonisins.
کلیدواژه‌ها
Fusarium؛ Deoxynivalenol؛ Fumonisin؛ Fungi؛ mycotoxin
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مراجع
  1. Oatley JT, Rarick MD, Ji GE, Linz JE. Binding of aflatoxin B1 to bifidobacteria in vitro. Journal of food protection. 2000;63(8):1133-6.
  2. Olopade BK, Oranusi SU, Nwinyi OC, Gbashi S, Njobeh PB. Occurrences of Deoxynivalenol, Zearalenone and some of their masked forms in selected cereals from Southwest Nigeria. NFS Journal. 2021;23:24-9.
  3. Ali Anvar SA, Nowruzi B, Afshari G. A Review of the Application of Nanoparticles Biosynthesized by Microalgae and Cyanobacteria in Medical and Veterinary Sciences. Iranian Journal of Veterinary Medicine. 2023;17(1).
  4. Weaver AC, Adams N, Yiannikouris A. Invited Review: Use of technology to assess and monitor multimycotoxin and emerging mycotoxin challenges in feedstuffs. Applied Animal Science. 2020;36(1):19-25.
  5. Hosseinabadi E, Garoussi MT, Khosravi AR, Gharagozloo F, Toosi BK, Moosakhani F. Prevalence of Prototheca and Fungal Contamination of Bulk Milk Tank of Industrial Dairy Cattle Herds in Iran. Iranian Journal of Veterinary Medicine. 2022;16(2).
  6. Khosravi AR, Shokri H, Taghipour BT, Yahya RR, Ghiasi M. A comparative study of mycoflora of Iranian and imported soybeans. 2008.
  7. Lutsky I, Mor N. Alimentary toxic aleukia (septic angina, endemic panmyelotoxicosis, alimentary hemorrhagic aleukia): t-2 toxin-induced intoxication of cats. The American Journal of Pathology. 1981;104(2):189.
  8. Goswami S, Mukit A, Bhattacharya M. Histochemical study on chronic aflatoxicosis in ducks. 2012.
  9. Hassan ZU, Al Thani R, Balmas V, Migheli Q, Jaoua S. Prevalence of Fusarium fungi and their toxins in marketed feed. Food Control. 2019;104:224-30.
  10. Pleadin J, Vahčić N, Perši N, Ševelj D, Markov K, Frece J. Fusarium mycotoxins' occurrence in cereals harvested from Croatian fields. Food control. 2013;32(1):49-54.
  11. Stanciu O, Juan C, Berrada H, Miere D, Loghin F, Mañes J. Study on trichothecene and zearalenone presence in Romanian wheat relative to weather conditions. Toxins. 2019;11(3):163.
  12. Chen C, Turna NS, Wu F. Risk assessment of dietary deoxynivalenol exposure in wheat products worldwide: Are new codex DON guidelines adequately protective? Trends in Food Science & Technology. 2019;89:11-25.
  13. Simsek S, Burgess K, Whitney KL, Gu Y, Qian SY. Analysis of deoxynivalenol and deoxynivalenol-3-glucoside in wheat. Food Control. 2012;26(2):287-92.
  14. Pokrzywa P, Surma M. Level of contamination with deoxynivalenol, zearalenone and fumonisins in cereal products–assessment of consumer exposure. Annals of Agricultural and Environmental Medicine. 2022;29(1):72.
  15. Kim KH. Microalgae Building Enclosures: Design and Engineering Principles: Routledge; 2022.
  16. Khosravi A, Shokri H, Yahyaraeyat R, Soltani M. Isolation of toxigenic and nontoxigenic fungi from feedstuff referred to the center of mycology. J Vet Res. 2004;59:221-6.
  17. Sarrafi O, Faezi Ghasemi M, Chaichi Nosrati A. Isolation and characterization of toxicogenic fungi strains from wheat and corn used in Kerman city. Journal of Microbial World. 2016;8(4):330-6.
  18. Alemzadeh E, Haddad R, Ahmadi A-R. Phytoplanktons and DNA barcoding: characterization and molecular analysis of phytoplanktons on the Persian Gulf. Iranian journal of microbiology. 2014;6(4):296.
  19. Molina A, Chavarría G, Alfaro-Cascante M, Leiva A, Granados-Chinchilla F. Mycotoxins at the start of the food chain in Costa Rica: Analysis of six Fusarium toxins and ochratoxin a between 2013 and 2017 in animal feed and aflatoxin M1 in dairy products. Toxins. 2019;11(6):312.
  20. Roberts HL, Bionaz M, Jiang D, Doupovec B, Faas J, Estill CT, et al. Effects of deoxynivalenol and fumonisins fed in combination to beef cattle: Immunotoxicity and gene expression. Toxins. 2021;13(10):714.
  21. Pierzgalski A, Bryła M, Kanabus J, Modrzewska M, Podolska G. Updated review of the toxicity of selected Fusarium toxins and their modified forms. Toxins. 2021;13(11):768.
  22. Sec FCPG. 683.100 Action Levels for Aflatoxins in Animal Food. Food and Drug Administration: Silver Spring, MD, USA. 2019.
  23. Holanda DM, Kim SW. Mycotoxin occurrence, toxicity, and detoxifying agents in pig production with an emphasis on deoxynivalenol. Toxins. 2021;13(2):171.
  24. Recommendation C. 2016/1319 of 29 July 201 Amending Recommendation 2006/576/EC as Regards Deoxynivalenol, Zearalenone and Ochratoxin A in Pet Food (Text. with EEA relevance), C/2016/4863. Publications Office of the European Union Brussels, Belgium; 2016.
  25. Simões D, Carbas B, Soares A, Freitas A, Silva AS, Brites C, et al. Assessment of Agricultural Practices for Controlling Fusarium and Mycotoxins Contamination on Maize Grains: Exploratory Study in Maize Farms. Toxins. 2023;15(2):136.
  26. Topi D, Babič J, Pavšič-Vrtač K, Tavčar-Kalcher G, Jakovac-Strajn B. Incidence of Fusarium mycotoxins in wheat and maize from Albania. Molecules. 2020;26(1):172.
  27. Castañares E, Martínez M, Cristos D, Rojas D, Lara B, Stenglein S, et al. Fusarium species and mycotoxin contamination in maize in Buenos Aires province, Argentina. European Journal of Plant Pathology. 2019;155(4):1265-75.
  28. Kokkonen M, Ojala L, Parikka P, Jestoi M. Mycotoxin production of selected Fusarium species at different culture conditions. International journal of food microbiology. 2010;143(1-2):17-25.
  29. Ghiasian SA, Rezayat SM, Kord-Bacheh P, Maghsood AH, Yazdanpanah H, Shephard GS, et al. Fumonisin production by Fusarium species isolated from freshly harvested corn in Iran. Mycopathologia. 2005;159:31-40.
  30. Seirafinia M, Omidi A, Rasooli A, Mohebbi M, Hoseinzadeh S. Influence of low-temperature stress on the production of zearalenone by three Fusarium species in vitro. Journal of Horticulture and Postharvest Research. 2021;4(1):63-70.
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