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تاثیر همزمان نانوسید، قارچکش و قارچ تریکودرما بر گیاهچههای کلزا مبتلا به پوسیدگی سفید ساقه ناشی از Sclerotinia sclerotiorum | ||
| مهار زیستی در گیاهپزشکی | ||
| دوره 10، شماره 2، اسفند 1401، صفحه 109-124 اصل مقاله (654.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.363789.350 | ||
| نویسندگان | ||
| فاطمه سلطانی نژاد* 1؛ کامران رهنما2؛ سید جواد صانعی3 | ||
| 1دانشجوی دکتری، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. | ||
| 2دانشیار،گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. | ||
| 3استادیار، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. | ||
| چکیده | ||
| در این بررسی، اثر بازدارندگی گونه های تریکودرما به همراه نانوسید و قارچ کش برای مبارزه تلفیقی با قارچ بیمارگر Sclerotinia sclerotiorum مورد مطالعه قرار گرفته است. بدین منظور، اثر جداگانه سه جدایه Trichoderma harzianum، یک جدایهT. virens، یک جدایه T. atroviride و یک جدایهT. koningii بر سه جدایه عامل بیمارگر (SS1، SS2 و SS3) در محیط کشت PDA دربردارنده 5 سطح از نانوسید(0، 30، 60، 100 و 130 پی پی ام)، به روش کشت متقابل در آزمایشگاه بررسی شد. سپس اثر T. harzianum (1211)، T. koningii (iso2) و T. virens (6011) به همراه نانوسید (سطوح 10 و 30 پی پی ام) و قارچ کش ایپرودیون–کاربندازیم (سطوح 2 و 5 پیپیام) بر یک جدایه عامل بیمارگر درشرایط گلخانه در قالب طرح کاملاً تصادفی با چهار تکرار، مورد ارزیابی قرار گرفت. در کشت متقابل، کمترین درصد بازدارندگی از رشد قارچ اسکلروتینیا، 6 درصد بود که متعلق به تیمارS. sclerotiorum (SS2) × Trichoderma virens (6011) در سطح 10 پی پی ام نانوسید بود. در گلخانه، تریکودرما به دو روش پوشش سطحی بذر و نیز مخلوط با خاک، بکار برده شد و صفات مورد ارزیابی شامل ارتفاع بوته و وزن تر و خشک گیاه بود. در آزمایش اول به روش پوشش سطحی بذر با سوسپانسیون گونه های تریکودرما، بیشترین میزان وزن تر گیاه به تیمارT. harzianum (1211) و بیشترین ارتفاع نیز به تیمار T. koningii (iso2) مربوط بود. در آزمایش دوم به روش کاربرد گونه های تریکودرما به صورت مخلوط با خاک، بیشترین وزن تر و خشک گیاه در تیمار T. harzianum (1211) + 30 پی پی ام نانوسید و بیشترین ارتفاع در تیمار T. harzianum (1211) + 2 پی پی ام قارچ(P≤0.01) مشاهده شد. در مجموع، تیمارهایی که گونه های تریکودرما به تنهایی به کار رفته بود با تیمارهایی که نانوسید و قارچ کش هم اضافه شده بود، تفاوت معنی داری را در وزن تر، خشک و ارتفاع گیاه نشان نداد. به علاوه کاربرد گونه های تریکودرما به تنهایی نسبت به تلفیق آن با نانوسید و قارچ کش، تاثیر بهتری بر صفات رویشی گیاهچه های کلزای مبتلا به این بیماری داشت. | ||
| کلیدواژهها | ||
| قارچکش ایپرودیون-کاربندازیم؛ گونههای تریکودرما؛ مدیریت تلفیقی؛ نانوسید؛ Sclerotinia sclerotiorum | ||
| عنوان مقاله [English] | ||
| Simultaneous effects of Nanocide, Fungicide and Trichoderma fungi on the Canola Seedlings Affected by Stem Rot Pathogen, Sclerotinia sclerotiorum | ||
| نویسندگان [English] | ||
| fatemeh soltani nezhad1؛ kamran rahnama2؛ seyed javad sanei3 | ||
| 11.,Ph.D. Student,, Department of Plant Protection Faculty of Plant production, Agricultural Sciences and Natural Resources of Gorgan University, Iran. | ||
| 2Associated Professor, Department of Plant Protection Faculty of Plant production, Agricultural Sciences and Natural Resources of Gorgan University, Iran. | ||
| 3Assistant Professor, Department of Plant Protection Faculty of Plant production, Agricultural Sciences and Natural Resources of Gorgan University, Iran. | ||
| چکیده [English] | ||
| In this research, the compatibility of Trichoderma species along with Nanocide and fungicide for the integrated management of this pathogenic fungi Sclerotinia sclerotiorum, has been studied. For this purpose, the effect of three isolates of Trichoderma harzianum, one isolate of T. virens, one isolate of T. atroviride and one isolate of T. koningii on the three isolates of pathogenic agent (SS1, SS2, SS3) in PDA medium containing 5 levels of Nanocide (0,30,60,100,130) by dual culture technique in the laboratory condition were studied. Then the effects of T. harzianum (1211), T koningii (iso2) and T. virens (6011) with two levels of Noniocid (10 & 30 ppm) and Iprodione+carbendazim fungicide (2 & 5 ppm) on one isolate of pathogenic agent in greenhouse condition, were evaluated. The lowest inhibition of mycelia growth of S. sclerotiorum in PDA medium with Nanocide, was 6%, that indicated for treatment S. sclerotiorum (SS2) × T. virens (6011) at level 10 ppm. In greenhouse condition, Trichoderma spp were tested in two stages, seed coated with Trichoderma conidia cell suspension and use Trichoderma mixed with soil. The evaluated parameters were height and wet and dry weight of the plant. The first experiment, was seed coated with Trichoderma conidia cell suspension, the most fresh weight was for T. harzianum (1211) as control and the most height was for T. koningii (iso2) alone. The second experiment, was for Trichoderma mixed with soil showed a significant increase in the most fresh and dry weight were for T. harzianum (1211) + 30 ppm Nanocide treatment and the most height of seedlings was also for T. harzianum (1211) + 2 ppm Iprodione–carbendazim (P≤0.01(. the statistical grouping showed that the treatments in which Trichoderma species were used alone showed no significant difference with treatments with Nanocid and fungicide in the wet and dry weight and height plant. Beside, using Trichoderma alone rather than the combination of Nanocid and fungicide, showed the better effect on growth parameters of the infected canola. | ||
| کلیدواژهها [English] | ||
| Integrated management, Sclerotinia sclerotiorum, Trichoderma, Nanocid, Iprodione+carbendazim fungicide | ||
| مراجع | ||
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