اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,041
تعداد مقالات34,022
تعداد مشاهده مقاله46,265,797
تعداد دریافت فایل اصل مقاله77,403,430
Taghizadeh, Marzieh, Sabagh Nekonam, Marzieh, Setorki, Mahbubeh. (1403). Production of Phenolic Compounds in Iberis amara L. Cell Suspension Culture under Chitosan Treatment. سامانه مدیریت نشریات علمی, 13(4), 1092-1102. doi: 10.22034/jmpb.2023.362412.1568
Marzieh Taghizadeh; Marzieh Sabagh Nekonam; Mahbubeh Setorki. "Production of Phenolic Compounds in Iberis amara L. Cell Suspension Culture under Chitosan Treatment". سامانه مدیریت نشریات علمی, 13, 4, 1403, 1092-1102. doi: 10.22034/jmpb.2023.362412.1568
Taghizadeh, Marzieh, Sabagh Nekonam, Marzieh, Setorki, Mahbubeh. (1403). 'Production of Phenolic Compounds in Iberis amara L. Cell Suspension Culture under Chitosan Treatment', سامانه مدیریت نشریات علمی, 13(4), pp. 1092-1102. doi: 10.22034/jmpb.2023.362412.1568
Taghizadeh, Marzieh, Sabagh Nekonam, Marzieh, Setorki, Mahbubeh. Production of Phenolic Compounds in Iberis amara L. Cell Suspension Culture under Chitosan Treatment. سامانه مدیریت نشریات علمی, 1403; 13(4): 1092-1102. doi: 10.22034/jmpb.2023.362412.1568

Production of Phenolic Compounds in Iberis amara L. Cell Suspension Culture under Chitosan Treatment

Journal of Medicinal plants and By-products
مقاله 19، دوره 13، شماره 4، اسفند 2024، صفحه 1092-1102    اصل مقاله (747.78 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2023.362412.1568
نویسندگان
Marzieh Taghizadeh1؛ Marzieh Sabagh Nekonam2؛ Mahbubeh Setorki* 3
1Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
2Kara Pajhouhesh Company, Isfahan Science and Technology Town, Isfahan, Iran
3Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
چکیده
Iberis amara L. medicinal herb is well-known for having pharmacological values although its use has been challenged by the low levels of secondary metabolites. For their bulk production, the present work aimed to investigate the effects of explants, different plant growth regulators, and photoperiod condition on the callus induction and cell suspension in I. amara, followed by investigating the chitosan effect on some secondary metabolites. A factorial experiment method based on completely randomized design with four replications was carried out. The optimum condition for induced callus achieved from the leaf explants in Murashige and Skoog (MS) media supplemented with 3 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 1-naphthalene acetic acid (NAA) under 16-h light/8-h dark photoperiod. The MS enhanced with 3 mg/L BAP, 1 mg/L NAA, and 2% (w/v) sucrose appeared to be the optimum conditions for suspension establishment. Thus, the cells were exposed to different concentrations of chitosan (200, 100, 50, and 0 mg/l) in their exponential growth stage from day 8 to 12 and day 12 to 16 following sub-cultures (T1) and sub-cultures (T2), respectively. The contents of phenolics and malondialdehyde (MDA) were then examined by UV-Vis spectrophotometer. The results showed that 50 mg/Lchitosan significantly improved the total phenol, flavonoid, flavonol, and anthocyanin content in the I. amara in a dose-dependent manner. The highest malondialdehyde (MDA) amount, as a result of lipid peroxidation, was observed under the 200 ppm chitosan elicitation. Overall, these novel findings demonstrated the possibility of applying the cell suspension of I. amara treated with chitosan as a helpful approach for improving synthesizing phenolic compounds under controlled and sterile conditions.
کلیدواژه‌ها
Cell suspension culture؛ Chitosan؛ Medicinal herbs؛ Secondary metabolite
مراجع
  1. Liu X-y., Ou H., Gregersen H. Ultrasound-assisted supercritical CO2 extraction of cucurbitacin E from Iberis amara seeds. Industrial Crops and Products. 2020; 145:112093.
  2. Golkar P., Moattar F. Essential Oil Composition, Bioactive Compounds, and Antioxidant Activities in Iberis amara L.. Natural Product Communications. 2019; 14:1934578X19846355.
  3. Weidner C., Rousseau M., Plauth A., Wowro S.J., Fischer C., Abdel-Aziz H., Sauer S. Iberis amara extract induces intracellular formation of reactive oxygen species and inhibits colon cancer. PLoS One. 2016; 11:e0152398.
  4. Khayyal M.T., Agha A.M., Zaki H.F., El-Sahar A., Abdel-Aziz H. Mechanisms involved in the anti-inflammatory and vascular effects of Iberis amara extract. Planta Medica. 2015; 81:1097-1102.
  5. Schempp H., Totha A., Weiser D., Elstner E.F. Antioxidative properties of Iberis amara extracts in biochemical model reactions. Arzneimittelforschung. 2015;53:568-577.
  6. Dias M.I., Sousa M.J., Alves R.C., Ferreira I.C. Exploring plant tissue culture to improve the production of phenolic compounds: A review. Indust. Crops and Prod. 2016; 82:9-22.
  7. Khvatkov P., Chernobrovkina M., Okuneva A., Shvedova A., Chaban I., Dolgov S. Callus induction and regeneration in Wolffia arrhiza (L.) Horkel ex Wimm. Plant Cell, Tissue and Organ. Culture (PCTOC). 2015; 120:263-273.
  8. Yue W., Ming Q-l., Lin B., Rahman K., Zheng C-J., Han T., Qin L-p. Medicinal plant cell suspension cultures: pharmaceutical applications and high-yielding strategies for the desired secondary metabolites. Critical Reviews in Biotechnol. 2016; 36:215-232.
  9. Taghizadeh M., Nasibi F., Kalantari K.M., Mohseni-Moghadam M. Modification of phytochemical production and antioxidant activity of Dracocephalum kotschyi cells by exposure to static magnetic field and magnetite nanoparticles. Plant Cell, Tissue and Organ. Culture (PCTOC). 2021; 34:1-13.
  10. Qiu H., Su L., Wang H., Zhang Z. Chitosan elicitation of saponin accumulation in Psammosilene tunicoides hairy roots by modulating antioxidant activity, nitric oxide production and differential gene expression. Plant Physiology and Biochem. 2021.
  11. Ramirez-Estrada K., Vidal-Limon H., Hidalgo D., Moyano E., Golenioswki M., Cusidó R.M., Palazon J. Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules. 2021; 21:182.
  12. Putalun W., Luealon W., De-Eknamkul W., Tanaka H., Shoyama Y. Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L. Biotechnol. letters. 2007; 29:1143-1146.
  13. Orlita A et al. Application of chitin and chitosan as elicitors of coumarins and furoquinolone alkaloids in Ruta graveolens L.(common rue). Biotechnology and Applied Biochemistry. 2008; 51:91-96.
  14. Udomsuk L., Jarukamjorn K., Tanaka H., Putalun W. Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation. Biotechnol. Letters. 2011; 33:369-374.
  15. Chakraborty M., Karun A., Mitra A. Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera. J. Plant Physiology. 2009; 166:63-71.
  16. Khan W., Prithiviraj B., Smith D.L. Chitosan and chitin oligomers increase phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities in soybean leaves. J. Plant Physiology. 2003; 160:859-863.
  17. Govindaraju S., Arulselvi P.I. Effect of cytokinin combined elicitors (l-phenylalanine, salicylic acid and chitosan) on in vitro propagation, secondary metabolites and molecular characterization of medicinal herb–Coleus aromaticus Benth (L). J. the Saudi Society of Agric. Sci. 2003; 17:435-444.
  18. Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum. 1962; 15:473-497.
  19. Afshar B., Golkar P. Mucilage synthesis by in vitro cell culture in different species of Alyssum Biotechnologia. J. Biotechnol. Computational Biology and Bionanotechnol. 2016; 97.
  20. Taghizadeh M., Nasibi F., Kalantari K.M., Ghanati F. Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation. Plant Cell, Tissue and Organ. Culture (PCTOC). 2019; 136:489-498.
  21. Tahsili J., Sharifi M., Safaie N., Esmaeilzadeh-Bahabadi S., Behmanesh M. Induction of lignans and phenolic compounds in cell culture of Linum album by culture filtrate of Fusarium graminearum. J. Plant Int. 2014; 9:412-417.
  22. Hara M., Oki K., Hoshino K., Kuboi T. Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyl. Plant Sci. 2003; 164:259-265.
  23. Heath R.L., Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochem. Biophysics. 1968; 125:189-198.
  24. Rikiishi K., Matsuura T., Maekawa M., Takeda K. Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos. Breeding Sci. 2008; 58:129-135.
  25. Garcia R., Pacheco G., Falcao E., Borges G., Mansur E. Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L.(Passifloraceae). Plant Cell, Tissue and Organ. Culture (PCTOC). 2011; 106:47-54.
  26. Taghizadeh M., Nasibi F., Kalantari K.M., Benakashani F. Callogenesis optimization and cell suspension culture establishment of Dracocephalum polychaetum Bornm. and Dracocephalum kotschyi Boiss.: An in vitro approach for secondary metabolite production. South African J. Botany. 2020; 132:79-86.
  27. Hajati R.J., Payamnoor V., Bezdi K.G., Chashmi N.A. Optimization of callus induction and cell suspension culture of Betula pendula Roth for improved production of betulin, betulinic acid, and antioxidant activity. In Vitro Cellular & Developmental Biology-Plant. 2016; 52:400-407.
  28. Mathur S., Shekhawat G.S. Establishment and characterization of Stevia rebaudiana (Bertoni) cell suspension culture: an in vitro approach for production of stevioside. Acta Physiologiae Plantarum. 2013; 35:931-939.
  29. Golkar P, Taghizadeh M., Yousefian Z. The effects of chitosan and salicylic acid on elicitation of secondary metabolites and antioxidant activity of safflower under in vitro salinity stress. Plant Cell, Tissue and Organ. Culture (PCTOC). 2019; 137:575-585.
  30. Talukder P., Talapatra S., Ghoshal N., Sen Raychaudhuri S. Antioxidant activity and high‐performance liquid chromatographic analysis of phenolic compounds during in vitro callus culture of Plantago ovata Forsk. and effect of exogenous additives on accumulation of phenolic compounds. J. Sci. of Food and Agric. 2016; 96:232-244.
  31. Malerba M., Cerana R. Reactive oxygen and nitrogen species in defense/stress responses activated by chitosan in sycamore cultured cells. International J. Molecular Sci. 2015; 16:3019-3034.
  32. Manquián-Cerda K., Escudey M., Zúñiga G., Arancibia-Miranda N, Molina M., Cruces E. Effect of cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets grown in vitro. Ecotoxicol Environment. Safety. 2016; 133:316-326.
  33. Maqsood S., Benjakul S., Abushelaibi A., Alam A. Phenolic compounds and plant phenolic extracts as natural antioxidants in prevention of lipid oxidation in seafood: A detailed review. Comprehensive Reviews in Food Sci. Food Safety. 2014; 13:1125-1140.
  34. Alrawaiq N.S., Abdullah A. A review of flavonoid quercetin: metabolism, bioactivity and antioxidant properties. International J. PharmTech Res. 2014; 6:933-941.
  35. Falcone Ferreyra M.L., Rius S., Casati P. Flavonoids: biosynthesis, biological functions, and biotechnological applications. Frontiers in Plant Sci. 2012; 3:222.
  36. Chen H., Seguin P., Archambault A., Constan L., Jabaji S. Gene expression and isoflavone concentrations in soybean sprouts treated with chitosan. Crop Sci. 2009; 49:224-236.
  37. Park CH., Yeo H.J., Park Y.E., Chun S.W., Chung Y.S., Lee S.Y., Park S.U. Influence of chitosan, salicylic acid and jasmonic acid on phenylpropanoid accumulation in germinated buckwheat (Fagopyrum esculentum Moench). Foods. 2019; 8:153.
  38. Kim J-S., Lee B-H., Kim S-H., Oh K-H., Cho K.Y. Responses to environmental and chemical signals for anthocyanin biosynthesis in non-chlorophyllous corn (Zea mays L.) leaf. J. Plant Biology. 2006; 49:16-25.
  39. Philibert T., Lee B.H., Fabien N. Current status and new perspectives on chitin and chitosan as functional biopolymers. Applied Biochem. Biotechnol. 2017; 181:1314-1337.
آمار
تعداد مشاهده مقاله: 379
تعداد دریافت فایل اصل مقاله: 394


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب