| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,846 |
| تعداد مقالات | 32,300 |
| تعداد مشاهده مقاله | 41,386,841 |
| تعداد دریافت فایل اصل مقاله | 18,562,109 |
ارزیابی کارآیی قارچکش زیستی کانگمی (Bacillus subtilis WP) در مدیریت لکه‌موجی گوجه‌فرنگی | ||
| مهار زیستی در گیاه‌پزشکی | ||
| دوره 10، شماره 2، اسفند 1401، صفحه 81-93 اصل مقاله (437.91 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.363476.347 | ||
| نویسندگان | ||
| بنفشه صفایی فراهانی* 1؛ مجتبی مرادزاده اسکندری2؛ شهرام نعیمی3؛ مجید رخشنده رو4؛ محمدرضا حسینی5 | ||
| 1استادیار، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان فارس، سازمان تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایران. | ||
| 2استادیار، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران. | ||
| 3دانشیار، بخش تحقیقات کنترل بیولوژیک، موسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران. | ||
| 4محقق، بخش تحقیقات زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان فارس، سازمان تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایران. | ||
| 5محقق، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران. | ||
| چکیده | ||
| روش رایج مدیریت بیماری لکه موجی گوجه‌فرنگی در ایران استفاده از قارچکشهای شیمیایی است. در پژوهش حاضر کارایی قارچکش زیستی کانگ می با دوزهای مصرفی 0/75 در هزار، یک در هزار و 1/25 در هزار با قارچکش ایمنوکتادین تریس و شاهد بدون استفاده از قارچکش مقایسه شد. آزمایش در استان‌های فارس و خراسان رضوی و در قالب طرح بلوک‌های کامل تصادفی با پنج تیمار و هر تیمار مشتمل بر چهار تکرار انجام شد. محلول‌پاشی اول پس از مرحلة گلدهی، محلول‌پاشی دوم با مشاهدة اولین علائم بیماری و محلول‌پاشی سوم تا پنجم در بازه‌های زمانی هفت روزه انجام شد. ارزیابی تیمارها شش مرتبه و بر اساس گسترش لکه‌های ناشی از بیماری در برگ‌ها با استفاده از مقیاس ارزیابی پیشرفت بیماری و نمره‌دهی به آنها انجام و داده‌های حاصل با استفاده از نرم‌افزار SAS تجزیه و تحلیل آماری شد. نتایج نشان داد قارچکش بلکیوت بیشترین تأثیر را در کاهش شدت بیماری دارد و قارچکش زیستی کانگمی با دوز 1/25 در هزار بیشترین کارایی را در میان دوزهای مورد بررسی داشته و سطح زیر منحنی پیشرفت بیماری (AUDPC) را در مقایسه با شاهد در استان‌های فارس و خراسان رضوی به ترتیب 30/16% و 33/21% کاهش می‌دهد. با توجه به اثرات سوء استفاده از قارچکشهای شیمیایی برای سلامتی انسان و محیط زیست و کاهش شدت بیماری لکه موجی گوجه فرنگی پس از استفاده از قارچکش زیستی کانگ می، این فرآورده با دوز 1/25 در هزار در مزارع گوجه فرنگی کشور به منظور تولید محصول سالم قابل استفاده است. | ||
| کلیدواژه‌ها | ||
| آلترناریا؛ خراسان رضوی؛ فارس؛ کنترل زیستی؛ محصول سالم | ||
| عنوان مقاله [English] | ||
| Evaluation of efficacy of CangMei biofungicide (Bacillus subtilis WP) for management of tomato early blight | ||
| نویسندگان [English] | ||
| Banafsheh Safaiefarahani1؛ Mojtaba Moradzadeh Eskandari2؛ Shahram Naeimi3؛ Majid Rakhshandehroo4؛ Mohammad Reza Hosseini5 | ||
| 1Assistant Professor, Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran | ||
| 2Assistant Professor, Department of Plant Protection, Khorasan-Razavi Center for Research of Agricultural Science and Natural Resources Center, AREEO, Mashhad, Iran. | ||
| 3Associate Professor, Department of Biological Control Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran. | ||
| 4Researcher, Horticulture Crops Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran. | ||
| 5Researcher, Department of Plant Protection, Khorasan–Razavi Center for Research of Agricultural Science and Natural Resources Center, AREEO, Mashhad, Iran. | ||
| چکیده [English] | ||
| Application of chemical fungicides is the primary method of tomato early blight disease management in Iran. In this study, the efficacy of CangMei biofungicide in doses of 0.75, 1 and 1.25 ml L–1 were assessed on the control of this disease alongside with Iminoctadine Tris and control without the use of fungicide. The experiment was carried out in Fars and Khorasan Razavi provinces according complete randomized blocks with five treatments and four replicates. The first spraying was performed after flowering stage, the second one was after the first symptoms of the disease was observed and repeated every 7 days up to 5 times. The effects of pesticides were assessed six times on the development of the disease on the leaves based on disease severity and the data was analyzed using SAS software. The results showed the best treatment to control of tomato early blight disease was Belkute®. Cang Mei bio fungicide in dose of 1.25 ml L–1 had the greatest effect among examined doses and reduced Area under disease progress curve (AUPDC) 30.16% and 33.21% in Fars and Khorasan Razavi respectively. Due to the destructive effects of chemical fungicides, Cang Mei could be recommended in dose of 1.25 ml L–1 to produce healthy crop. . | ||
| کلیدواژه‌ها [English] | ||
| Alternaria sp, Biocontrol, Fars, Healthy crop, Khorasan Razavi | ||
| مراجع | ||
|
Abbasi, P.A. & Weselowski, B. 2014. Influence of foliar sprays of Bacillus subtilis QST 713 on development of early blight disease and yield of field tomatoes in Ontario. Canadian Journal of Plant Pathology, 36(2): 170–178.
Abdalla, S.A., Algam, S.A., Ibrahim, E.A. & Naim, A.M.E. 2014. In vitro screening of Bacillus isolates for biological control of early blight disease of tomato in shambat soil. World Journal of Agricultural Research, 2(2): 47–50.
Adinarayana, M., Narasegowda, N. & Devappa, V. 2018. Effect of Bacillus subtilis var. amyloliquefaciens on Growth, Yield and Control of Early Blight in Tomato (Solanum lycopersicum L.). International Journal of Current Microbiology and Applied Sciences, 7(2): 656–664.
Alabouvette, C., Olivain, C. & Steinberg, C. 2006. Biological control of plant diseases: the European situation. European Journal of Plant Pathology, 114(3): 329–341.
Anand, T., Chandrasekaran, A., Kuttalam, S., & Samiyappan, R. 2010. Evaluation of azoxystrobin (Amistar 25 SC) against early leaf blight and leaf spot diseases of tomato. Journal of Agricultural Technology, 6(3): 469–485.
Arunakumara, K.T., Kulkarni, M.S., Thammaiah, N. & Hegde, Y. 2010. Fungicidal management of early blight of tomato. Indian Phytopathology, 63(1): 96–97.
Ashwini, N. & Srividya, S. 2014. Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1. Biotech, 4(2): 127–136.
Awan, Z.A., & Shoaib, A. 2019. Combating early blight infection by employing Bacillus subtilis in combination with plant fertilizers. Current Plant Biology, 20(1): 1–8.
Ayilara, M.S., Adeleke, B.S., Akinola, S.A., Fayose, C.A., Adeyemi, U.T., Gbadegesin, L.A., Omole, R.K., Johnson, R.M., Uthman, Q.O. & Babalola, O.O. 2023. Biopesticides as a promising alternative to synthetic pesticides: A case for microbial pesticides, phytopesticides, and nanobiopesticides. Frontiers in Microbiology, 14: 1–16.
Azimi, H. 2014. Effect of Chlorothalonil and Famoxadone + Cymoxanil in control of early blight disease of tomato under field conditions. Journal of Applied Research in Plant Protection, 3(1): 35–48 (In Persian with English Summary).
Azimi, H. & Sharifi, K. 2009. Investigation on the effects of Chlorothalonil for control of tomato Early blight disease. Final Report of Research Project. Agricultural Research and Education and extention Organization, 23p (In Persian with English Summary).
Basamma, R.H. & Kulkarni, S. 2017. Growth promoting ability and bioefficacy of Bacillus subtilis against powdery mildew and early blight of tomato through foliar spray in pot culture studies. Journal of Pharmacognosy and Phytochemistry, 6(2): 244–246.
Campbell, C.L. & Madden, L.V. 1990. Introduction to Plant Disease Epidemiology. John Wiley and Sons, New York.
Chaerani, R. & Voorrips, R.E. 2006. Tomato early blight (Alternaria solani): the pathogen, genetics, and breeding for resistance. Journal of General Plant Pathology, 72(6): 335–347.
Chowdappa, P. Kumar, S.M., Lakshmi, M.J. & Upreti, K.K. 2013. Growth stimulation and induction of systemic resistance in tomato against early and late blight by Bacillus subtilis OTPB1 or Trichoderma harzianum OTPB3. Biological Control, 65(1): 109–117.
de Jensen, C.E., Percich, J.A. & Graham, P.H. 2002. Integrated management strategies of bean root rot with Bacillus subtilis and Rhizobium in Minnesota. Field Crops Research, 74(3): 107–115.
Derbalah, A.S., Kamel, S.M., Morsy, S.Z. & El–Sawy, M.M. 2012. Alternatives to control powdery mildew and early blight diseases of tomato under greenhouse conditions. Egyptian Journal of Biological Pest Control, 22(2): 185–190.
El–Fiki, I.A.I. 2017. Evaluation of some fungitoxicants for controlling tomato early blight disease. Journal of Phytopathology and Pest Management, 4(2): 38–52.
Espinosa–Vázquez, M.Á., Espinoza–Medinilla, E.E., Orantes–García, C., Garrido–Ramírez, E. & Rioja–Paradela, T.M. 2019. Salicylic acid and Bacillus subtilis as control of early blight (Alternaria solani) in tomato plants (Solanum lycopersicum). Revista de la Facultad de Ciencias Agrarias UNCuyo, 51(1): 161–171.
Farooq, S., Jat, R.R., Gupta, A., Singh, R., Majeed, M., Nabi, S.U., Bashir, N. & Shah, O.S. 2019. Evaluation of different fungicides against Alternaria solani (Ellis & Martin) Sorauer cause of early blight of tomato under laboratory conditions. The Pharma Innovation Journal, 8(5):140–142
Furuya, S., Mochizuki, M., Aoki, Y., Kobayashi, H., Takayanagi, T., Shimizu, M. & Suzuki, S. 2011. Isolation and characterization of Bacillus subtilis KS1 for the biocontrol of grapevine fungal diseases. Biocontrol Science and Technology, 21(6): 705–720.
Gondal, A.S., Ijaz, M., Riaz, K. & Khan, A.R. 2012. Effect of different doses of fungicide (Mancozeb) against alternaria leaf blight of tomato in tunnel. Journal of Plant Pathology and Microbiology, 3(1): 1–3.
Gould, W.A. 2013. Tomato Production, Processing and Technology. Elsevier Science, Cambridge.
Grigolli, J.F.J., Kubota, M.M., Alves, D.P., Rodrigues, G.B., Cardoso, C.R., Silva, D.J.H.D. & Mizubuti, E.S.G. 2011. Characterization of tomato accessions for resistance to early blight. Crop Breeding and Applied Biotechnology, 11(1): 174–180.
Hiraoka, H., Asaka, O., Ano, T. & Shoda, M. 1992. Characterization of Bacillus subtilis RB14, coproducer of peptide antibiotics iturin A and surfactin. The Journal of General and Applied Microbiology, 38(6): 635–640.
Jones, J.B., Zitter, T.A., Momol, T.M. & Miller, S.A. 2014. Compendium of Tomato Diseases and Pests. APS Press, New York.
Kajimura, Y., Sugiyama, M. & Kaneda, M. 1995. Bacillopeptins, new cyclic lipopeptide antibiotics from Bacillus subtilis FR–2. The Journal of Antibiotics, 48(10): 1095–1103.
Khan, M.A., Rashid, A., &,Iqbal, M.J. 2003. Evaluation of foliar applied fungicides against early blight of potato under field conditions. International Journal of Agriculture and Biology, 5(5): 543–544.
Kumar, V., Singh, G. & Tyagi, A. 2017. Evaluation of different fungicides against Alternaria leaf blight of tomato (Alternaria solani). International Journal of Current Microbiology and Applied Sciences, 6(6): 2343–2350.
Lastochkina, O., Seifikalhor, M., Aliniaeifard, S., Baymiev, A., Pusenkova, L., Garipova, S., Kulabuhova, D. & Maksimov, I. 2019. Bacillus spp.: efficient biotic strategy to control postharvest diseases of fruits and vegetables. Plants, 8(4): 1–24.
Liu, H., Pan, X., Zhang, X. & Wang, J. 1995. Experiments on Bacillus strains producing antagonistic proteins. Chinese Journal of Biological Control, 11(1): 160–164.
Liu, K., Qi, Z., Tan, L., Yang, C. & Hu, C. 2023. Mixed Use of Chemical Pesticides and Biopesticides among Rice–Crayfish Integrated System Farmers in China: A Multivariate Probit Approach. Agriculture, 13(8): 1590–1607.
Mahdavi, M., Eslahi, M. & Karampoor, F. 2015a. Study on the efficacy of Cabrio Due (EC 11/2%) in the control of early blight of tomato. Agricultural Research and Education and extention Organization, 35p (In Persian with English Summary).
Mahdavi, M., Eslahi, M. & Karampoor, F. 2015b. Study of the efficacy of Signum (WG 33/4%) in control of early blight of tomato. Agricultural Research and Education and extention Organization, 34p (In Persian with English Summary).
Metz, N. 2017. Biologicals for the control of Alternaria solani under greenhouse and field conditions. Sixteenth Euroblight Workshop, Aarhus, Denmark, Special Report, 85–90.
Moges, M.M., val Selvaraj, T. & Jebessa, M.T. 2012. Influence of some antagonistic bacteria against early blight (Alternaria solani (Ell. & Mart.) Jones & Grout.) of tomato (Lycopersicon esculentum Mill.). The African Journal of plant Science and Biotechnology, 6(1): 40–44.
Montealegre, J.R., Reyes, R., Pérez, L.M., Herrera, R., Silva, P. & Besoain, X. 2003. Selection of bioantagonistic bacteria to be used in biological control of Rhizoctonia solani in tomato. Electronic Journal of Biotechnology, 6(2): 115–127.
Morsy, E.M., Abdel–Kawi, K.A. & Khalil, M.N.A. 2009. Efficiency of Trichoderma viride and Bacillus subtilis as biocontrol agents against Fusarium solani on tomato plants. Egyptian Journal of Phytopathology, 37(1): 47–57.
Muthukumar, A. & Udhayakumar, R. 2017. Evaluation of new molecules of fungicides against early blight (Alternaria solani (Ellis and Martin) Jones and Grout) of tomato (Lycopersicon esculentum Mill). International Journal of Chemical Studies, 5(5): 597–600.
Naeimi, Sh., Firoozjahi, F., Marzban, R., Khosravi, V., Noori, M., Shokri, R., Omrani, M. & Mohadasi, A. 2016. Evaluation of CangMei, a commercial biofungicide containing Bacillus subtilis, in controlling rice blast disease under paddy field conditions. Agricultural Research and Education and extention Organization, 23p (In Persian with English Summary).
Nasr Esfahani, M. 2011. Studies on the effect of flint fungicide on the control of early blight of potato. Agricultural Research and Education and extention Organization, 20p (In Persian with English Summary).
Nasr Esfahani, M. 2015. Efficacy of iminoctadine tris (WP 40%) Fungicide against Potato Early Blight Disease, Alternaria solani (Fr.) Keissler and A. alternata Sorauer. Pesticides in Plan Protection Sciences 2: 31–43. (In Persian with English Summary).
Nasr Esfahani, M., Naderpour, S. & Rafizadeh, N. 2010. Studies on fungistatic effects of some fungicides on growth of Alternaria alternata and A. solani. Plant Protection Journal, 2(4), 311–332. (In Persian with English Summary).
Pandey, K.K., Pandey, P.K., Kalloo, G. & Banerjee, M.K. 2003. Resistance to early blight of tomato with respect to various parameters of disease epidemics. Journal of general plant pathology, 69(6): 364–371.
Pane, C. & Zaccardelli, M. 2015. Evaluation of Bacillus strains isolated from Solanaceous phylloplane for biocontrol of Alternaria early blight of tomato. Biological Control, 84(1): 11–18.
Pertot, I., Puopolo, G., Giovannini, O., Angeli, D., Sicher, C. & Perazzolli, M. 2016. Advantages and limitations involved in the use of microbial biofungicides for the control of root and foliar phytopathogens of fruit crops. Italus Hortus, 23(3): 3–12.
Raid, R.N. 1990. Evaluation of fungicides for control of early blight in Florida celery production. Florida State Horticultural Society 102: 360–362.
Ramezani, Y., Taheri, P. & Mamarabadi, M. 2019. Identification of Alternaria spp. associated with tomato early blight in Iran and investigating some of their virulence factors. Journal of Plant Pathology, 101(3): 647–659.
Rettinassababady, C. & Jeyalakshmi, C. 2014. Bio–fungicides: The best alternative for sustainable food security and ecosystem. Microbial Diversity and Biotechnology in Food Security, (14): 401–411.
Sadana, D. & Didwania, N. 2015. Bioefficacy of fungicides and Trichoderma harzianum against Alternaria solani causing early blight of tomato. Journal of Agroecology and Natural Resource Management, 2(3): 181–186.
Samada, L.H. & Tambunan, U.S.F. 2020. Biopesticides as promising alternatives to chemical pesticides: A review of their current and future status. OnLine Journal of Biological Science, 20(2): 66–76.
Sharifi, K. Nasr–Esfahani, M. & Moradzadeh Eskandari, M. 2015. Study on the efficacy of Consento (SC 45%) in controling the early blight of tomato. Agricultural Research and Education and extention Organization, 29p (In Persian with English Summary).
Singh, V.P., Khan, R.U. & Devesh, P. 2018. In vitro evaluation of fungicides, bio–control agents and plant extracts against early blight of tomato caused by Alternaria solani (Ellis and Martin) Jones and Grout. International Journal of Plant Protection, 11(1): 102–108.
Smith, T.J., Blackman, S.A. & Foster, S.J. 2000. Autolysins of Bacillus subtilis: multiple enzymes with multiple functions. Microbiology, 146(2): 249–262.
Stein, T. 2005. Bacillus subtilis antibiotics: structures, syntheses and specific functions. Molecular microbiology, 56(4): 845–857.
Tolentino Júnior, J.B., Rezende, R., Itako, A.T., Freitas, P.S.L.D. & Frizzone, J.A. 2011. Drip fungigation in early blight control of tomato. Acta Scientiarum. Agronomy, 33(1): 9–14.
Van Driesche, R.G., Carruthers, R.I., Center, T., Hoddle, M.S., Hough–Goldstein, J., Morin, L., Smith, L., Wagner, D.L., Blossey, B., Brancatini, V. & Casagrande, R. 2010. Classical biological control for the protection of natural ecosystems. Biological Control, 54(1): 2–33.
Yadav, V.K., Kumar, V. & Mani, A. 2018. Evaluation of fungicides, biocontrol agents and plant extracts against early blight of potato caused by Alternaria solani. International Journal of Chemical Studies, 6(1): 1227–1230. | ||
|
آمار تعداد مشاهده مقاله: 323 تعداد دریافت فایل اصل مقاله: 255 |
||