| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,838 |
| تعداد مقالات | 32,218 |
| تعداد مشاهده مقاله | 41,133,075 |
| تعداد دریافت فایل اصل مقاله | 18,373,118 |
بررسی تاثیر پیش‌تیمار اسید سالیسیلیک بر جوانه‌زنی بذرهای زوال یافته و آنزیم‌های آنتی‌اکسیدانی گیاه کدوی پوست کاغذی تحت تنش خشکی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 13، شماره 2 - شماره پیاپی 34، تیر 1403، صفحه 33-46 اصل مقاله (325.66 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2023.362518.1491 | ||
| نویسنده | ||
| نساء قره باغلی* | ||
| گروه علوم کشاورزی، دانشگاه پیانور، تهران، ایران. | ||
| چکیده | ||
| با هدف ارزیابی اثر اسید سالیسیلیک در بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی در شرایط تنش خشکی، آزمایشی به شکل فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار به اجرا درآمد. پیش‌تیمار غلظت‌های مختلف اسید سالیسیلیک شامل صفر (پیش تیمار شده با آب)، 1 و 5/1 میلی‌مولار در سطوح مختلف خشکی صفر، 2/0-، 4/0- و 6/0- مگاپاسکال مورد بررسی قرار گرفت. شاخص های متوسط زمان جوانه‌زنی، درصد جوانه‌زنی، طول گیاهچه، بنیه بذر، نشت الکترولیتی غشاء، قندهای محلول، پروتئین‌های محلول، مالون دی‌آلدهید، فعالیت آسکوربات پراکسیداز، سوپراکسید دیسموتاز و آنزیم‌های کاتالاز مورد ارزیابی قرار گرفتند. نتایج نشان داد پیش‌تیمار بذرها با غلظت‌های مختلف اسید سالیسیلیک، از کاهش معنی‌دار شاخص‌های جوانه‌زنی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی جلوگیری نمود. به‌طوری‌که در پتانسیل 6/0- مگاپاسکال، پیش‌تیمار بذرها با 5/1 میلی‌مولار اسید سالیسیلیک درصد جوانه‌زنی، شاخص بنیه بذر، قندها و پروتئین‌های محلول را به‌ترتیب 2/80، 4/169، 6/51، 4/93 درصد و برای فعالیت آسکوربات پراکسیداز، سوپراکسید دیسموتاز و آنزیم‌های کاتالاز را به‌ترتیب 2/41، 12 و 8/11 درصد نسبت به شاهد افزایش داد. بر اساس یافته‌های تحقیق پیش‌تیمار اسید سالیسیلیک با غلظت 5/1 میلی‌مولار به منظور کاهش تنش اکسیداتیو ناشی از خشکی برای بذر های زوال یافته کدوی پوست کاغذی توصیه می‌شود. | ||
| کلیدواژه‌ها | ||
| اسید سالیسیلیک؛ پروتئین‌های محلول؛ تنش اکسیداتیو؛ درصد جوانه‌زنی | ||
| عنوان مقاله [English] | ||
| Investigating the effect of pretreatment of salicylic acid on the germination of aged pumpkin (Cucurbita pepo L.) seeds and antioxidant enzymes of plant under drought stress | ||
| نویسندگان [English] | ||
| Nesa Gharehbaghli | ||
| Department of Agriculture sciences, Payame Noor Unvierstiy, Tehran, Iran. | ||
| چکیده [English] | ||
| With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic acid including zero (pretreated with water), 1 and 1.5 mM at different drought levels of zero, -0.2, -0.4 and -0.6 MPa were investigated. Indicators of mean germination time, germination percentage, seedling length, seed vigor, membrane electrolyte leakage, soluble carbohydrates, soluble proteins, malondialdehyde, ascorbate peroxidase activity, superoxide dismutase and catalase enzymes were evaluated. The results showed that the pretreatment of seeds with different concentrations of salicylic acid prevented the significant decrease in the germination indices of the deteriorated pumpkin seeds under drought stress. So that at the potential of -0.6 MPa, the treatment of seeds with 1.5 mM salicylic acid increased the percentage of germination, seed germination index, soluble carbohydrates and soluble proteins respectively 80.2, 169.4, 51.6, 93.4% and for the activity of ascorbate peroxidase, superoxide dismutase and catalase enzymes, 41.2%, 12% and 11.8%, respectively, compared to the control. Based on the research findings, pretreatment of salicylic acid (1.5 mM) is recommended in order to reduce the oxidative stress caused by drought for pumpkin. | ||
| کلیدواژه‌ها [English] | ||
| germination percentage, oxidative stress, salicylic acid, soluble proteins | ||
| مراجع | ||
|
Abdollahi, F., E.R.K. Sari, and L. Jafari. 2016. Effect of salicylic acid priming on seed germination and some vegetative and physiological characteristics of zinnia (Zinnia elegans) under drought condition. Iran. J. Seed Sci. Res. 3:105-116. DOR:20.1001.1.24763780.1395.3.4.9.9. (In Persian, with English Abstract) Ahmad, F., A. Kamal, A. Singh, F. Ashfaque, S. Alamri, and M.H. Siddiqui. 2020. Salicylic acid modulates antioxidant system, defense metabolites, and expression of salt transporter genes in Pisum sativum under salinity stress. J. Plant Growth Regul. 41: 1905-1918.DOI:10.1007/s00344-020-10271-5. Ahmad, I., T. Khaliq, A. Ahmad, S.M.A. Basra, Z. Hasnain, and A. Ali. 2012. Effect of seed priming with ascorbic acid, salicylic acid and hydrogen peroxide on emergence, vigor and antioxidant activities of maize. Afr. J. Biotechnol. 11: 1127-1132. DOI: 10.5897/AJB11.2266. Alam, A., H. Ullah, N. Thuenprom, R. Tisarum, S. Cha-um, and A. Datta. 2022. Seed priming with salicylic acid enhances growth, physiological traits, fruit yield, and quality parameters of cantaloupe under water-deficit stress. S. Afr. J. Bot. 150: 1-12. DOI:10.1007/s42729-023-01314-3. Al-Sahil, A.A. 2016. Effect of gibberellic and salicylic acids on seed germination attributes of cucumber (Cucumis sativus L.) under induced salt stress. Cercetari Agronomice in Moldova. 1: 99-109. DOI:10.1515/cerce-2016-0009. Apon, T.H., S.F Ahmed, Z.F. Bony, M.R. Chowdhury, J.F. Asha, and A. Biswas. 2023. Sett priming with salicylic acid improves salinity tolerance of sugarcane (Saccharum officinarum L.) during early stages of crop development. Helyon. 9: 1-14. DOI:10.1016/j.heliyon.2023.e16030. Azooz, M.M., A.M. Youssef, and P. Ahmad. 2011. Comparative evaluation of salicylic acid on growth, osmotic solutes and antioxidant enzyme activities on broad bean seedlings grown under diluted seawater. Int. J. Plant Physiol. Biochem. 3: 253-264. DOI:10.5897/IJPPB11.052. Behnam, A., H. Abbaspour, and F. Saeid Nematpour. 2019. Effects of salicylic acid on growth improvement and changes of biochemical parameters of wheat seedlings in cadmium stress. J. Plant Res. 32: 315-327. DOR: 20.1001.1.23832592.1398.32.2.1.6. (In Persian, with English Abstract) Bradford, M.M. 1976. A dye binding assay for protein. Anal. Biochem.72: 248-254. DOI:10.1006/abio.1976.9999. Cakmak, I., and W. Horst. 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiol. 83: 463–468. DOI:10.1111/j.1399-3054.1991.tb00121.x. Cavalcanti, F.R., J.T.A. Oliveira, A.S. Martins-Miranda, R.A. Viégas, and J.A.G. Silveira. 2004. Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpeas leaves. New Phytol. 163: 563-571. DOI:10.1111/j.1469-8137.2004.01139.x. Chen, S., C.B. Zhao, R.M. Ren, and J.H. Jiang. 2023. Salicylic acid had the potential to enhance tolerance in horticultural crops against abiotic stress. Front. Plant Sci. 14:1-9. DOI:10.3389/fpls.2023.1141918. Delouche, J.C., and C.C. Baskin. 1973. Accelerated ageing technique for predicting relative storability of seed lots. Seed Sci. Technol. 1: 427-452. El-Beltagi, H.S., and H. Mohammad. 2013. Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism.Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 41: 44-57. DOI:10.15835/nbha4118929. Ellis, R.A., and E.H. Roberts. 1981. The quantification of ageing and survival inorthodox seeds. Seed Sci. Technol. 9: 373-409. Gharib, F.A., and A.Z. Hegazi. 2010. Salicylic acid ameliorates germination, seedling growth, phytohormone and enzymes activity in bean (Phaseolus vulgaris L.) under cold stress. J. Am. Sci. 6: 675-683. González, E.M. 2023. Drought Stress Tolerance in Plants. Int. J. Mol. Sci. 24: 1-3. DOI:10.3390/ijms24076562. Hampton, J.G., and D.M. TeKrony. 1995. Handbook of Vigour Test Methods. The international Seed Testing Association, Zurich, Switzerland. Hayat, Q., S. Hayat, M. Irfan, and A. Ahmad. 2010. Effect of exogenous salicylic acid under changing environment: A review. Environ. Exp. Bot. 68: 14-25. DOI:10.1016/j.envexpbot.2009.08.005. Huang, C., A. Qin, Y. Gao, S. Ma, Z. Liu, B. Zhao, and Liu, Z. 2023. Effects of water deficit at different stages on growth and ear quality of waxy maize. Front. Plant Sci. 14: 1-14. DOI:10.3389/fpls.2023.1069551. Irigoyen, J.J., D.W. Emerich, and M. Sanchez-Diaz.1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant. 84: 55-60. DOI:10.1111/j.1399-3054.1992.tb08764.x. ISTA. 2007. International Rules for Seed Testing. Seed Sci. Technol. 13: 299-520. Jisha, K.C., K. Vijayakumari, and J.T. Puthur. 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiol. Plant. 35: 1381-1396. DOI: 10.1007/s11738-012-1186-5. Jumali, S.S., I.M. Said, I. Ismail, and Z. Zainal. 2011. Genes induced by high concentration of salicylic acid in Mitragyna speciosa.Aust. J. Crop Sci. 5: 296- 303. Kabiri, R., F. Nasibi, and H. Farahbakhsh. 2014. Effect of Exogenous Salicylic Acid on Some Physiological Parameters and Alleviation of Drought Stress in Nigella sativa Plant under Hydroponic Culture. Plant Prot. Sci. 50: 43-51. DOI:10.17221/56/2012-PPS. Khosravi, S., A. Baghizadeh, and M.T. Nezami. 2011. The salicylic acid effect on the Salvia officianlis L. sugar, protein and proline contents under salinity (NaCl) stress. J. Stress Physiol. Biochem. 7: 80-87. Macedo, C., A.M. Silva, A.S. Ferreira, M.M. Moreira, C. Delerue-Matos, and F. Rodrigues. 2022. Microwave-and ultrasound-assisted extraction of Cucurbita pepo seeds: A Comparison study of antioxidant activity, phenolic profile, and in-vitro cells effects. Appl. Sci. 12: 1-18. DOI:10.3390/app12031763. Michel, B.E., and M.R. Kaufmann. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916. DOI: 10.1104/pp.51.5.914. Mir-Mahmoudi, T., S. Karbalaye Golizadeh, N. Khaliliqhdam, and S. Yazdanseta. 2014. The effect of salicylic acid on rate germination and seedling establishment on rapeseed (Brassica napus L.).Int. J. Agric. Innovations Res. 2: 1122-1125. DOI:10.15835/nsb12310777. Nakano, Y., and K. Asada. 1981. Hydrogen peroxide scavenged by ascrobate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22: 867–880. DOI: 10.1093/oxfordjournals.pcp.a076232. Rouhi, H.R., M.H. Vafaei, M. Saman, and A. Abbasi Surki. 2021. Effect of Hydrogen Peroxide on Physiological Quality and Germination of Aged Pumpkin Seeds under Drought Stress Condition. Philipp. Agric. Scientist. 104(1): 90-99. Savita, K., and S. Jakhar. 2015. Effect of pre-treatment of chickpea (Cicer arietinum L.) seeds on seed germination and seedling growth under salt stress. Int. J. Curr. Adv. Res. 3: 303-311 Sedghi, M., H.K. Basiri, and R.S. Sharifi. 2013. Effects of salicylic acid on the antioxidant enzymes activity in sunflower. Ann.West Un. Timişoara Ser. Biol. 16: 67-72. Sepehri, A., and H.R. Rouhi. 2016. Enhancement of seed vigor performance in aged groundnut (Arachis hypogaea L.) seeds by sodium nitroprusside under drought stress. Philipp. Agric. Scientist. 99(4): 339-347. Sharafizad, M., A. Naderi, S.A. Siadat, T. Sakinejad, and S. Lak. 2013. Effect of Salicylic Acid Pretreatment on Germination of Wheat under Drought Stress. J. Agric. Sci. 5: 179-199. DOI:10.5539/jas.v5n3p179. Sharafizadeh, M. 2017. Effect of Salicylic Acid and Drought Stress on Germination and Activity of Antioxidant Enzymes of Barely. Iranian J. Seed Sci. Technol. 6: 161-169. DOI: 10.22034/IJSST.2018.116567. (In Persian, with English Abstract) Sheykhbaglou, R., S. Rahimzadeh, O. Ansari, and M. Sedghi. 2013. The Effect of SalicylicAcid and Gibberellin on Seed Reserve Utilization, Germination and Enzyme Activity of Sorghum (Sorghum bicolor L.) Seeds under Drought Stress. J. Stress Physiol. Biochem. 10: 5-13. Shraiy, A.M.E. and A.M. Hegazi. 2009. Effect of acetyl salicylic acid, indole-3- bytric acid and gibberellic acid on plant growth and yield of pea (Pisum Sativum L.). Aust. J. Basic Appl. Sci. 3: 3514-3523. Song, W., A. Zheng, H. Saho, L. Chu, M. Brestic, and Z. Zhang. 2012. The alleviative effect of salicylic acid on the physiological indices of the seedling leaves in six different wheat genotypes under lead stress. Plant Omics J. 5: 486-493. Tabatabaei, S.A. 2013. The Effect of priming on germination and enzyme activity of sesame (Sesamum indicum L.) seeds after accelerated aging. J. Physiol. Biochem. 9: 132-138. Wang, D., H. Xiao, X. Lyu, H. Chen, and F. Wei. 2023. Lipid oxidation in food science and nutritional health: A comprehensive review. Oil Crop Sci. 8: 35-44. DOI: 10.1016/j.ocsci.2023.02.002. Yan, M. 2015. Hydropriming promotes germination of aged napa cabbage seeds. Seed Sci. Technol. 43: 303-307. DOI: 10.15258/sst.2015.43.2.12. Zadehbagheri, M. 2014. Salicylic acid priming in corn (Zea mays L.var. Sc.704) Reinforces NaCl tolerance at germination and the seedling growth stage. Int. J. Biosci. 4: 187-197. DOI: 10.12692/ijb/4.5.187-197. Zhang, Z., and Q. Shang. 2010. Promoting effect of salicylic acid and chitosan on germination of cucumber seeds under NaCl stress. ChinaVegetables. 1: 26-29. | ||
|
آمار تعداد مشاهده مقاله: 218 تعداد دریافت فایل اصل مقاله: 133 |
||