اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,036
تعداد مقالات33,971
تعداد مشاهده مقاله45,666,889
تعداد دریافت فایل اصل مقاله76,594,420
AliNejad Kasbakhi, Noura, Vavrincová, Diana, Cepcova, Diana. (1403). In vitro effect of 1-methyltryptophan isomers on epithelial-mesenchymal transition transcription factors in tubular epithelial cells after ischemia-reperfusion injury. سامانه مدیریت نشریات علمی, 79(2), 307-314. doi: 10.32592/ARI.2024.79.2.307
Noura AliNejad Kasbakhi; Diana Vavrincová; Diana Cepcova. "In vitro effect of 1-methyltryptophan isomers on epithelial-mesenchymal transition transcription factors in tubular epithelial cells after ischemia-reperfusion injury". سامانه مدیریت نشریات علمی, 79, 2, 1403, 307-314. doi: 10.32592/ARI.2024.79.2.307
AliNejad Kasbakhi, Noura, Vavrincová, Diana, Cepcova, Diana. (1403). 'In vitro effect of 1-methyltryptophan isomers on epithelial-mesenchymal transition transcription factors in tubular epithelial cells after ischemia-reperfusion injury', سامانه مدیریت نشریات علمی, 79(2), pp. 307-314. doi: 10.32592/ARI.2024.79.2.307
AliNejad Kasbakhi, Noura, Vavrincová, Diana, Cepcova, Diana. In vitro effect of 1-methyltryptophan isomers on epithelial-mesenchymal transition transcription factors in tubular epithelial cells after ischemia-reperfusion injury. سامانه مدیریت نشریات علمی, 1403; 79(2): 307-314. doi: 10.32592/ARI.2024.79.2.307

In vitro effect of 1-methyltryptophan isomers on epithelial-mesenchymal transition transcription factors in tubular epithelial cells after ischemia-reperfusion injury

Archives of Razi Institute
مقاله 10، دوره 79، شماره 2، خرداد و تیر 2024، صفحه 307-314    اصل مقاله (651.52 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.2.307
نویسندگان
Noura AliNejad Kasbakhi؛ Diana Vavrincová* ؛ Diana Cepcova
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic
چکیده
The compound 1-methyltryptophan (1-MT) has been shown to act protectively in renal ischemia-reperfusion injury. Toll-like receptor 4 (TLR-4) signaling is also a regular process of epithelial to mesenchymal transition (EMT) that can after Ischemia-reperfusion injury (IRI) result in as an increase of renal fibrosis. EMT is associated with specific transcription factors – Snai1, Snai2, Zeb1 and Twist. 1-MT could regulate EMT and act as antifibrotic agent. This study aimed to investigate the effect of 1-MT on EMT transcription factors in tubular epithelial cells that underwent 30 min. Renal tubular epithelial cells (TECs) were isolated from Lewis rats using a standard protocol with Fe2O3 magnetic separation and selective media as previously mentioned. ischemia and 48 hours of reperfusion. Cells were cultivated and divided into 4 groups: C-TECs– control cells, IRI-TECs – IRI-induced TECs, D-IRI-TECs – IRI-induced TECs treated with 1-methyl-D-tryptophan, L-IRI-TECs – IRI-induced TECs treated with 1-methyl-L-tryptophan. IRI was induced in all groups for 30 min by mineral oil (except for C-TECs) followed by 48 hours of reperfusion. RNA and proteins were isolated from harvested cells. Using semi-quantitative PCR (RT-sqPCR) we assessed the relative mRNA expression of EMT transcription factors Snai1, Snai2, Zeb1, and Twist. Hereby we have shown, that the treatment of ischemia-induced TECs with both 1-MT isomers lowers the expression of EMT transcription factors Snai1 and Zeb1 that were increased by ischemia and reperfusion of TECs. This could act favorably in renal IRI decreasing EMT and renal fibrosis, therefore showing the potential of 1-MT as a part of therapy in renal transplantation aimed at renal ischemia-reperfusion injury.
کلیدواژه‌ها
Gene expression؛ ischemia؛ renal fibrosis؛ mRNA؛ kidney
سایر فایل های مرتبط با مقاله
مراجع
  1. Webster AC, Nagler EV, Morton RL, Masson P. Chronic kidney disease. The lancet. 2017;389(10075):1238-52.
  2. Motamedi S, Asghari A, Jahandideh A, Abedi G, Mortazavi P. Effects of Echinacea Purpurae Extract on Testicular Ischemia/Reperfusion (I/R) Injury in Rat. Iranian Journal of Veterinary Medicine, 2019; 13(3): 303-313.
  3. Ghotbitabar Z, Asghari A, Hassanpour S, Jahandide A. Effects of Quebracho Tannin Extract on Testicular Ischemia-/Reperfusion. Iranian Journal of Veterinary Medicine. 2022; 16(4): 423-431.
  4. Kalogeris T, Baines CP, Krenz M, Korthuis RJ. Cell biology of ischemia/reperfusion injury. International review of cell and molecular biology. 2012;298:229-317.
  5. Kellum JA, Romagnani P, Ashuntantang G, Ronco C, Zarbock A, Anders HJ. Acute kidney injury. Nature reviews Disease primers. 2021;7(1):52.
  6. Sánchez-Tilló E, Liu Y, de Barrios O, Siles L, Fanlo L, Cuatrecasas M, Darling DS, Dean DC, Castells A, Postigo A. EMT-activating transcription factors in cancer: beyond EMT and tumor invasiveness. Cellular and molecular life sciences. 2012;69:3429-56.
  7. Stemmler MP, Eccles RL, Brabletz S, Brabletz T. Non-redundant functions of EMT transcription factors. Nature cell biology. 2019;21(1):102-12.
  8. Klahr S. Mechanisms of progression of chronic renal damage. J Nephrol. 1999;12: S53–S62.
  9. Becker JG. Hewitson TD. The role of tubulointerstitial injury in chronic renal failure. Curr Opin Nephrol Hypertens. 2000; 9: 133–138.
  10. Wirthgen E, Leonard AK, Scharf C, Domanska G. The immunomodulator 1-methyltryptophan drives tryptophan catabolism toward the kynurenic acid branch. Frontiers in immunology. 2020;11:313.
  11. Metz R, Duhadaway JB, Kamasani U, Laury-Kleintop L, Muller AJ, Prendergast GC. 2007. Novel tryptophan catabolic enzyme IDO2 is the preferred biochemical target of the antitumor indoleamine 2,3-dioxygenase inhibitory compound D-1-methyl-tryptophan. Cancer Res. 2007;67(15):7082-7.
  12. Čepcová D, Kema IP, Sandovici M, Deelman LE, Šišková K, Klimas J, Vavrinec P, Vavrincová-Yaghi D. The protective effect of 1-methyltryptophan isomers in renal ischemia-reperfusion injury is not exclusively dependent on indolamine 2, 3-dioxygenase inhibition. Biomedicine & Pharmacotherapy. 2021;135:111180.
  13. Nieto MA. The snail superfamily of zinc-finger transcription factors. Nature reviews Molecular cell biology. 2002;3(3):155-66.
  14. Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial–mesenchymal transition. Nature reviews Molecular cell biology. 2014;15(3):178-96.
  15. Ribatti D, Tamma R, Annese T. Epithelial-mesenchymal transition in cancer: a historical overview. Translational oncology. 2020;13(6):100773.
  16. Peinado H, Quintanilla M, Cano A. Transforming growth factor β-1 induces snail transcription factor in epithelial cell lines: mechanisms for epithelial mesenchymal transitions. Journal of Biological Chemistry. 2003;278(23):21113-23.
  17. Liu J, Zhong Y, Liu G, Zhang X, Xiao B, Huang S, Liu H, He L. Role of Stat3 signaling in control of EMT of tubular epithelial cells during renal fibrosis. Cellular Physiology and Biochemistry. 2017;42(6):2552-8.
  18. Grande MT, Sánchez-Laorden B, López-Blau C, De Frutos CA, Boutet A, Arévalo M, Rowe RG, Weiss SJ, López-Novoa JM, Nieto MA. Snail1-induced partial epithelial-to-mesenchymal transition drives renal fibrosis in mice and can be targeted to reverse established disease. Nature medicine. 2015;21(9):989-97.
  19. Lee SW, Won JY, Kim WJ, Lee J, Kim KH, Youn SW, Kim JY, Lee EJ, Kim YJ, Kim KW, Kim HS. Snail as a potential target molecule in cardiac fibrosis: paracrine action of endothelial cells on fibroblasts through snail and CTGF axis. Molecular Therapy. 2013;21(9):1767-77.
  20. Jayachandran A, Königshoff M, Yu H, Rupniewska E, Hecker M, Klepetko W, Seeger W, Eickelberg O. SNAI transcription factors mediate epithelial–mesenchymal transition in lung fibrosis. Thorax. 2009;64(12):1053-61.
  21. Xiong M, Jiang L, Zhou Y, Qiu W, Fang L, Tan R, Wen P, Yang J. The miR-200 family regulates TGF-β1-induced renal tubular epithelial to mesenchymal transition through Smad pathway by targeting ZEB1 and ZEB2 expression. American journal of physiology-renal physiology. 2012;302(3):F369-79.
  22. Yao L, Conforti F, Hill C, Bell J, Drawater L, Li J, Liu D, Xiong H, Alzetani A, Chee SJ, Marshall BG. Paracrine signalling during ZEB1-mediated epithelial–mesenchymal transition augments local myofibroblast differentiation in lung fibrosis. Cell Death & Differentiation. 2019;26(5):943-57.
  23. Cruz-Solbes AS, Youker K. Epithelial to mesenchymal transition (EMT) and endothelial to mesenchymal transition (EndMT): role and implications in kidney fibrosis. Kidney Development and Disease, Springer. 2017;60:345-372.
  24. Sun S, Ning X, Zhang Y, Lu Y, Nie Y, Han S, Liu L, Du R, Xia L, He L, Fan D. Hypoxia-inducible factor-1α induces Twist expression in tubular epithelial cells subjected to hypoxia, leading to epithelial-to-mesenchymal transition. Kidney international. 2009;75(12):1278-87.
  25. Matheus, LH. Simão, GM. Amaral, TA. Brito, RB. et al. 2017. Indoleamine 2, 3- dioxygenase (IDO) increases during renal fibrogenesis and its inhibition potentiates TGF-β 1- induced epithelial to mesenchymal transition. BMC Nephrology. 2017; 18: 287.
  26. Brito RB, Malta CS, Souza DM, Matheus LH, Matos YS, Silva CS, Ferreira JM, Nunes VS, França CM, Dellê H. 1-methyl-D-tryptophan potentiates TGF-β-induced epithelial-mesenchymal transition in T24 human bladder cancer cells. PLoS One. 2015;10(8):e0134858.
آمار
تعداد مشاهده مقاله: 12,352
تعداد دریافت فایل اصل مقاله: 788


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب