Ultraviolet (UV) rays are part of solar radiation, which induces physiological processes mediated by photoreceptors. This research investigated the effect of short-term exposure to different bands of UV rays (UV-A, UV-B, and UV-C) with the wavelengths of 365, 312, and 254 nm on Melissa officinalis L. seedlings, respectively. The amount of Chl. a, b, total, carotenoids, anthocyanin, UV absorbing compounds, and proteins in the leaves were measured. The control group was not treated with any UV rays. The total chlorophyll content decreased under UV-A (19%), UV-B (23%), and UV-C (49%) treatments. The lowest amount of Chl. a, b, and total chlorophyll belonged to UV-C with about 50, 46, and 49%, respectively. The carotenoid contents significantly decreased under UV-A and UV-C treatments. The ratio of carotenoids to total chlorophyll increased under UV-B (17%), and UV-C (45%) treatments compared with the control. The reduction of carotenoids content under UV treatment was less than that of the chlorophyll. The amount of anthocyanin and UV-absorbing compounds increased under different bands of UV radiation. The UV-A and UV-B increased protein contents, while UV-C treatment decreased protein content. This research indicated that the UV-B ray stimulate plant antioxidant system helping to plant survival under UV stress. |
- Nawkar G.M., Maibam P., Park J.H., Sahi V.P., Lee S.Y., Kang C.H. UV-induced cell death in plants. Inter J Molecular Sci. 2013;14(1):1608-1628.
- Kakani V., Reddy K., Zhao D., Sailaja K. Field crop responses to ultraviolet-B radiation: a review. Agric & Forest Meteorology. 2003;120(1-4):191-218.
- Loconsole D., Santamaria P. UV lighting in horticulture: A sustainable tool for improving production quality and food safety. Horticulturae. 2021;7(1):9.
- Heijde M., Ulm R. UV-B photoreceptor-mediated signalling in plants. Trends in Plant Sci. 2012; 17(4):230-237.
- Yadav A., Singh D., Lingwan M., Yadukrishnan P., Masakapalli S.K., Datta S. Light signaling and UV‐B‐mediated plant growth regulation. J Integrative Plant Biol. 2020; 62(9):1270-1292.
- Singh P., Singh A., Choudhary K.K. Revisiting the role of phenylpropanoids in plant defense against UV-B stress. Plant Stress. 2023:100143.
- Day T., Martin G., Vogelmann T. Penetration of UV‐B radiation in foliage: evidence that the epidermis behaves as a non‐uniform filter. Plant, Cell and Environment. 1993; 16(6):735-741.
- Julkunen-Tiitto R., Nenadis N., Neugart S., Robson M., Agati G., Vepsäläinen J., Zipoli G., Nybakken L., Winkler B., Jansen M.A. Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques. Phytochemistry Reviews. 2015;14:273-297.
- Pruvot G., Cuiné S., Peltier G., Rey P. Characterization of a novel drought-induced 34-kDa protein located in the thylakoids of Solanum tuberosum L. plants. Planta. 1996; 198(3):471-479.
- Sharma S., Chatterjee S., Kataria S., Joshi J., Datta S., Vairale M.G., Veer V. A review on responses of plants to UV‐B radiation related stress. UV‐B Radiation: From Environmental Stressor to Regulator of Plant Growth. 2017:75-97.
- Shakeri A., Sahebkar A., Javadi B. Melissa officinalis L.–A review of its traditional uses, phytochemistry and pharmacology. J. Ethnopharmacology. 2016;188:204-228.
- Hothem S.D., Marley K.A., Larson R.A. Photochemistry in Hoagland's nutrient solution. Journal of Plant Nutrition. 2003;26(4):845-854.
- Lichtenthaler H.K., Buschmann C. Extraction of phtosynthetic tissues: chlorophylls and carotenoids. Current protocols in food Analytical Chemistry. 2001; 1(1):F4. 2.1-F4. 2.6.
- Wagner G.J. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology. 1979; 64(1):88-93.
- Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976;72(1-2):248-254.
- León-Chan R.G., López-Meyer M., Osuna-Enciso T., Sañudo-Barajas J.A., Heredia J.B., León-Félix J. Low temperature and ultraviolet-B radiation affect chlorophyll content and induce the accumulation of UV-B-absorbing and antioxidant compounds in bell pepper (Capsicum annuum) plants. Environmental and Experimental Botany. 2017;139:143-151.
- Badmus U.O., Crestani G., Cunningham N., Havaux M., Urban O., Jansen M.A. UV Radiation induces specific changes in the carotenoid profile of Arabidopsis thaliana. Biomolecules. 2022;12(12):1879.
- Liu M., Cao B., Zhou S., Liu Y. Responses of the flavonoid pathway to UV-B radiation stress and the correlation with the lipid antioxidant characteristics in the desert plant Caryopteris mongolica. Acta Ecologica Sinica. 2012;32(3):150-155.
- Erkan M., Wang S.Y., Wang C.Y. Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in strawberry fruit. Postharvest Biology and Technology. 2008;48(2):163-171.
- Fedina I., Hidema J., Velitchkova M., Georgieva K., Nedeva D. UV-B induced stress responses in three rice cultivars. Biologia Plantarum. 2010;54:571-574.
- Enteshari S., Torkzadeh M., Manouchehr K.K., Ghorbanli M.L. The effect of different bands of ultraviolet radiation on pigments content in Glycine Max L. 2005;77-84.
- Devlin M., Withman F. CBS Publishers & distributors. Plant Physioly. 2002.
- Bijami A., Rezanejad F., Sasan H.A. The effects of post-harvest UV-B radiation on some antioxidant compounds, PAL activity and total protein contents of ripe red tomato (Lycopersicon esculentum). Iranian Journal of Plant Biology. 2011;2(6):29-38.
- Rezayi Far Z., Fallahi S., Gholinezhad E. The effect of drought stress and Ultraviolet on antioxidant defensive system of enzyme and non-enzyme in three varieties of wheat (Triticum aestivum L.). Journal of Plant Process and Function. 2018;7(24):155-170.
- Ranjbar A., Mousavi S.A. The effects of Enhanced Ultraviolet-B Radiation and Heavy Metal Cadmium on Some Physiological Parameters of Lettuce (Lactuca sativa). Plant Research Journal. 2018; 30(4):853-861.
- Hideg É., Jansen M.A., Strid Å. UV-B exposure, ROS, and stress: inseparable companions or loosely linked associates? Trends in plant Science. 2013; 18(2):107-115.
- Ayangbenro A.S., Babalola O.O. A new strategy for heavy metal polluted environments: a review of microbial biosorbents. International Journal of environmental Research and Public Health. 2017;14(1):94.
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