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Genetic and chemical diversity among Salvia multicaulis populations employing AFLP markers and the essential oil profile | ||
| Rostaniha | ||
| دوره 24، شماره 2 - شماره پیاپی 69، اسفند 2023، صفحه 139-150 اصل مقاله (548.72 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/bot.j.iran.2023.362816.1363 | ||
| نویسندگان | ||
| Golaleh Mostafavi* 1؛ Soheila Hesami2 | ||
| 1Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran | ||
| 2MSc Postgraduate, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| Salvia multicaulis is a widespread medicinal plant growing in north, northwest, and center of Iran. In this study, the aerial parts of 29 specimens belonging to five geographical populations were collected and dried at full flowering stage. AFLP molecular markers were applied for genetic diversity analysis. Essential oil was extracted by hydro-distillation and its chemical compositions were analyzed by GC/MS. AFLP results led to the recognition of two different genotypes. The results demonstrated high molecular affinity among Azerbaijan and Kurdistan populations. However, Tehran showed much more correlations with Isfahan and Kohgiluyeh-o-Boyerahmad populations. The AMOVA test results, demonstrated high within population genetic variability. STRUCTURE analysis revealed some degree of population genetic fragmentation that was principally due to genetic difference occurred between Azerbaijan and the rest of the studied populations. Overall, 40 constituents were determined among which, a-pinene (11.2–19.9%) and 1,8-cineole (10.2–20.4%) were distinguished as two major compounds. Tehran showed closer affinity to Isfahan and Kohgiluyeh-o-Boyerahmad. The results of molecular studies were in accordance with those obtained by the chemical compositions. Therefore, ecological factors and geographical distance as well as genetics play an important role in the species evolution and distribution in different parts of the country. | ||
| کلیدواژه‌ها | ||
| chemical polymorphism؛ GC/MS analysis؛ Lamiaceae؛ molecular affinity؛ molecular markers | ||
| عنوان مقاله [English] | ||
| تنوع ژنتیکی و شیمیایی در جمعیت‌های Salvia multicaulis با استفاده از مارکرهای AFLP و ویژگی‌های اسانس | ||
| نویسندگان [English] | ||
| گلاله مصطفوی1؛ سهیلا حسامی2 | ||
| 1استادیار گروه زیست‌شناسی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2دانش‌آموخته کارشناسی ارشد، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده [English] | ||
| Salvia multicaulis (نعناییان) گیاهی دارویی با پراکنش وسیع در شمال، شمال‌غرب و مرکز ایران است. در این مطالعه، بخش‌های هوایی 29 نمونه مربوط به پنج جمعیت جغرافیایی جمع‌آوری و در مرحله گل‌دهی کامل خشک شدند. مارکرهای مولکولی AFLP برای آنالیز تنوع ژنتیکی به کار برده شد. اسانس به وسیله تقطیر آبی استخراج و ترکیبات شیمیایی آن توسط آنالیز GC/MS شناسایی شدند. نتایج AFLP منجر به شناسایی دو ژنوتیپ متفاوت گردید. نتایج شباهت مولکولی بالایی بین جمعیت‌های آذربایجان و کردستان را نشان داد. با این حال، جمعیت تهران تشابه بیشتری به جمعیت‌های اصفهان و کهگیلویه و بویراحمد نشان داد. نتایج تست AMOVA تنوع بین جمعیتی بالایی را نشان داد. آنالیز STRUCTURE، درجاتی از قطعه قطعه شدن ژنتیکی جمعیت را آشکار ساخت که به طور کلی، به دلیل تفاوت ژنتیکی رخ داده بین جمعیت آذربایجان و سایر جمعیت‌های مورد مطالعه بود. در مجموع، 40 ترکیب شناسایی شد که در میان آن، آلفا-پینن (9/2-19/%11) و 1 و 8-سینئول (4/2-20/%10) به عنوان دو ترکیب اصلی تشخیص داده شدند. تهران بیشترین شباهت را به اصفهان و کهگیلویه و بویراحمد نشان داد. نتایج مطالعات مولکولی با نتایج به دست آمده از ترکیبات شیمیایی مطابقت داشت. بنابراین، فاکتورهای بوم‌شناختی و فاصله جغرافیایی و نیز ژنتیک نقش مهمی را در تکامل و انتشار گونه در نقاط مختلف کشور ایفا می‌کنند. | ||
| کلیدواژه‌ها [English] | ||
| آنالیز GC/MS, پلی‌مورفیسم شیمیایی, تشابه مولکولی, مارکرهای مولکولی, نعناییان | ||
| مراجع | ||
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